{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T19:36:51Z","timestamp":1780688211672,"version":"3.54.1"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Animal Reproduction Science"],"published-print":{"date-parts":[[2023,6]]},"DOI":"10.1016\/j.anireprosci.2023.107253","type":"journal-article","created":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T11:28:55Z","timestamp":1683890935000},"page":"107253","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Seasonal spermatogenesis in the red-eared slider (Trachemys scripta elegans): The roles of GnRH, actin cytoskeleton, and MAPK"],"prefix":"10.1016","volume":"253","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7850-2671","authenticated-orcid":false,"given":"Na","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1680-3812","authenticated-orcid":false,"given":"Wenzhuo","family":"Rao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyu","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Shahid","family":"Iqbal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haitao","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiling","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.anireprosci.2023.107253_bib1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.mce.2007.11.011","article-title":"Mitogen activated protein kinases (MAPKs) as regulators of spermatogenesis and spermatozoa functions","volume":"282","author":"Almog","year":"2008","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.1016\/j.anireprosci.2023.107253_bib2","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1530\/jrf.0.0900353","article-title":"Plasma sex hormone concentrations during the reproductive cycle in the male lizard, Podarcis s. sicula","volume":"90","author":"And\u00f2","year":"1990","journal-title":"J. Reprod. Fertil."},{"key":"10.1016\/j.anireprosci.2023.107253_bib3","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.ygcen.2011.03.007","article-title":"Role of estrogen in the regulation of spermatogenesis in the Indian wall lizard Hemidactylus flaviviridis","volume":"172","author":"Bharti","year":"2011","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.anireprosci.2023.107253_bib4","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1016\/j.theriogenology.2006.01.041","article-title":"Seasonally activated spermatogenesis is correlated with increased testicular production of testosterone and epidermal growth factor in mink (Mustela vison)","volume":"66","author":"Blottner","year":"2006","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2023.107253_bib5","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1002\/mrd.10151","article-title":"Effects of the aromatase inhibitor fadrozole on plasma sex steroid secretion, spermatogenesis and epididymis morphology in the lizard, Podarcis sicula","volume":"63","author":"Cardone","year":"2002","journal-title":"Mol. Reprod. Dev."},{"key":"10.1016\/j.anireprosci.2023.107253_bib6","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.ygcen.2019.03.013","article-title":"Testosterone trends within and across seasons in male humpback whales (Megaptera novaeangliae) from Hawaii and Alaska","volume":"279","author":"Cates","year":"2019","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.anireprosci.2023.107253_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/j.anireprosci.2020.106307","article-title":"Characteristics of seasonal spermatogenesis in the soft-shelled turtle","volume":"214","author":"Chen","year":"2020","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.anireprosci.2023.107253_bib8","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1002\/mrd.1151","article-title":"17beta-estradiol induces spermatogonial proliferation through mitogen-activated protein kinase (extracellular signal-regulated kinase 1\/2) activity in the lizard (Podarcis s. sicula)","volume":"61","author":"Chieffi","year":"2002","journal-title":"Mol. Reprod. Dev."},{"key":"10.1016\/j.anireprosci.2023.107253_bib9","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1095\/biolreprod.109.080135","article-title":"Role of apoptosis and cell proliferation in the testicular dynamics of seasonal breeding mammals: a study in the Iberian mole, Talpa occidentalis","volume":"83","author":"Dadhich","year":"2010","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.anireprosci.2023.107253_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.141744","article-title":"Modulation of the intestinal barrier adaptive functions in Red-eared slider (Trachemys scripta elegans) invading brackish waters","volume":"751","author":"Ding","year":"2021","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.anireprosci.2023.107253_bib11","doi-asserted-by":"crossref","first-page":"40225","DOI":"10.1074\/jbc.M105143200","article-title":"Signaling through extracellular signal-regulated kinase is required for spermatogonial proliferative response to stem cell factor","volume":"276","author":"Dolci","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.anireprosci.2023.107253_bib12","first-page":"1","article-title":"Reproductive cycles in lizards and snakes. Univ. Kans. publ","volume":"52","author":"Fitch","year":"1970","journal-title":"Mus. Nat. Hist."},{"key":"10.1016\/j.anireprosci.2023.107253_bib13","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0058276","article-title":"Transcriptome analysis of spermatogenically regressed, recrudescent and active phase testis of seasonally breeding wall lizards Hemidactylus flaviviridis","volume":"8","author":"Gautam","year":"2013","journal-title":"PLoS ONE"},{"key":"10.1016\/j.anireprosci.2023.107253_bib14","unstructured":"GISD, 2010, (Global Invasive Species Database) Species profile: Trachemys scripta elegans. http:\/\/www.iucngisd.org\/gisd\/speciesname\/Trachemys+scripta+elegans."},{"key":"10.1016\/j.anireprosci.2023.107253_bib15","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1530\/reprod\/119.2.271","article-title":"Chemical and thermal effects on the viability and motility of spermatozoa from the turtle epididymis","volume":"119","author":"Gist","year":"2000","journal-title":"J. Reprod. Fertil."},{"key":"10.1016\/j.anireprosci.2023.107253_bib16","doi-asserted-by":"crossref","first-page":"250","DOI":"10.4161\/spmg.1.3.18092","article-title":"Reptilian spermatogenesis: A histological and ultrastructural perspective","volume":"1","author":"Gribbins","year":"2011","journal-title":"Spermatogenesis"},{"key":"10.1016\/j.anireprosci.2023.107253_bib17","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1002\/jmor.10069","article-title":"Cytological evaluation of spermatogenesis and organization of the germinal epithelium in the male slider turtle, Trachemys scripta","volume":"255","author":"Gribbins","year":"2003","journal-title":"J. Morphol."},{"key":"10.1016\/j.anireprosci.2023.107253_bib18","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1002\/jmor.10872","article-title":"Ultrastructure of spermiogenesis in the American alligator, Alligator mississippiensis (Reptilia, Crocodylia, Alligatoridae)","volume":"271","author":"Gribbins","year":"2010","journal-title":"J. Morphol."},{"key":"10.1016\/j.anireprosci.2023.107253_bib19","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1016\/j.molcel.2016.10.016","article-title":"SRF co-factors control the balance between cell proliferation and contractility","volume":"64","author":"Gualdrini","year":"2016","journal-title":"Mol. Cell"},{"key":"10.1016\/j.anireprosci.2023.107253_bib20","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1002\/jmor.20050","article-title":"Seasonal spermatogenic cycle and morphology of germ cells in the viviparous lizard Mabuya brachypoda (Squamata, Scincidae)","volume":"273","author":"Hern\u00e1ndez-Franyutti","year":"2012","journal-title":"J. Morphol."},{"key":"10.1016\/j.anireprosci.2023.107253_bib21","doi-asserted-by":"crossref","first-page":"962","DOI":"10.3389\/fphys.2019.00962","article-title":"Adenosine monophosphate-activated protein kinase signaling regulates lipid metabolism in response to salinity stress in the Red-eared slider turtle Trachemys scripta elegans","volume":"10","author":"Hong","year":"2019","journal-title":"Front. Physiol."},{"key":"10.1016\/j.anireprosci.2023.107253_bib22","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/S0016-6480(02)00623-8","article-title":"Variation of plasma sex steroid concentrations in wild and captive populations of Hermann\u2019s tortoise (Testudo hermanni hermanni) in Southern France","volume":"130","author":"Huot-Daubremont","year":"2003","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.anireprosci.2023.107253_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.anireprosci.2020.106288","article-title":"Seasonal changes in the spermatogenesis of the large Japanese field mice (Apodemus speciosus) controlled by proliferation and apoptosis of germ cells","volume":"214","author":"Ito","year":"2020","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.anireprosci.2023.107253_bib24","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1159\/000439039","article-title":"Circannual testis changes in seasonally breeding mammals","volume":"9","author":"Jim\u00e9nez","year":"2015","journal-title":"Sex. Dev."},{"key":"10.1016\/j.anireprosci.2023.107253_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.ygcen.2020.113446","article-title":"The hypothalamic-pituitary-gonadal axis and thyroid hormone regulation interact to influence seasonal breeding in green anole lizards (Anolis carolinensis)","volume":"292","author":"Kang","year":"2020","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.anireprosci.2023.107253_bib26","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/0016-6480(81)90248-3","article-title":"Histochemical and biochemical investigation on the annual cycle of testis, epididymis, and plasma testosterone of the tortoise, Testudo hermanni hermanni Gmelin","volume":"44","author":"Kuchling","year":"1981","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.anireprosci.2023.107253_bib27","doi-asserted-by":"crossref","DOI":"10.4161\/21565562.2014.992654","article-title":"Testicular histology and germ cell cytology during spermatogenesis in the Mississippi map turtle, Graptemys pseudogeographica kohnii, from Northeast Arkansas","volume":"4","author":"Lancaster","year":"2014","journal-title":"Spermatogenesis"},{"key":"10.1016\/j.anireprosci.2023.107253_bib28","unstructured":"Lowe, S., Browne, M., Boudjelas, S., Poorter, M.D., 2000, 100 of the world\u2019s worst invasive alien species. A selection from the Global Invasive Species Database (GISD). published by the Invasive Species Specialist Group (ISSG) a specialist group of the Species Survival Commission (SSC) of the World Conservation Union (IUCN)."},{"key":"10.1016\/j.anireprosci.2023.107253_bib29","doi-asserted-by":"crossref","first-page":"958","DOI":"10.3390\/genes10120958","article-title":"Transcriptome sequencing reveals the traits of spermatogenesis and testicular development in large yellow croaker (Larimichthys crocea)","volume":"10","author":"Luo","year":"2019","journal-title":"Genes (Basel)"},{"key":"10.1016\/j.anireprosci.2023.107253_bib30","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.mce.2017.04.004","article-title":"Differential roles of PKC isoforms (PKCs) in GnRH stimulation of MAPK phosphorylation in gonadotrope derived cells","volume":"463","author":"Mugami","year":"2018","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.1016\/j.anireprosci.2023.107253_bib31","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1038\/s41419-019-1782-z","article-title":"Multiple signaling pathways in Sertoli cells: recent findings in spermatogenesis","volume":"10","author":"Ni","year":"2019","journal-title":"Cell Death Dis."},{"key":"10.1016\/j.anireprosci.2023.107253_bib32","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1006\/gcen.1999.7419","article-title":"Seasonal changes in sex and adrenal steroid hormones of gopher tortoises (Gopherus polyphemus)","volume":"117","author":"Ott","year":"2000","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.anireprosci.2023.107253_bib33","doi-asserted-by":"crossref","DOI":"10.1080\/21565562.2014.996025","article-title":"Endocrine control of spermatogenesis: Role of FSH and LH\/ testosterone","volume":"4","author":"Ramaswamy","year":"2014","journal-title":"Spermatogenesis"},{"key":"10.1016\/j.anireprosci.2023.107253_bib34","doi-asserted-by":"crossref","first-page":"1402","DOI":"10.1002\/jmor.21394","article-title":"The sexual segment of the kidney of a tropical rattlesnake, Crotalus durissus (Reptilia, Squamata, Viperidae), and its relationship to seasonal testicular and androgen cycles","volume":"282","author":"Resende","year":"2021","journal-title":"J. Morphol."},{"key":"10.1016\/j.anireprosci.2023.107253_bib35","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1016\/j.crvi.2017.10.001","article-title":"Testicular steroidogenic enzymes in the lizard Podarcis sicula during the spermatogenic cycle","volume":"340","author":"Rosati","year":"2017","journal-title":"C. R. Biol."},{"key":"10.1016\/j.anireprosci.2023.107253_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/j.ygcen.2020.113579","article-title":"Spermatogenesis and regulatory factors in the wall lizard Podarcis sicula","volume":"298","author":"Rosati","year":"2020","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.anireprosci.2023.107253_bib37","first-page":"1763","article-title":"The aromatase-estrogen system in the testes of non-mammalian vertebrates","volume":"11","author":"Rosati","year":"2021","journal-title":"Anim. (Basel)"},{"key":"10.1016\/j.anireprosci.2023.107253_bib38","doi-asserted-by":"crossref","first-page":"1770","DOI":"10.1126\/science.287.5459.1770","article-title":"Global biodiversity scenarios for the year 2100","volume":"287","author":"Sala","year":"2000","journal-title":"Science"},{"key":"10.1016\/j.anireprosci.2023.107253_bib39","unstructured":"Shine, R., 1985, The reproductive biology of Australian reptiles: A search for general patterns, in: Grigg, G., Shine, R. and Ehmann, H. (Eds.), Biology of the Australian Frogs and Reptiles. Surrey Beatty & Sons Pty Ltd., Chipping Norton New South Wales, pp. 297\u2013303."},{"key":"10.1016\/j.anireprosci.2023.107253_bib40","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1002\/jmor.1051980110","article-title":"Spermiogenesis in the Red-ear turtle (Pseudemys scripta) and the domestic fowl (Gallus domesticus): a study of cytoplasmic events including cell volume changes and cytoplasmic elimination","volume":"198","author":"Sprando","year":"1988","journal-title":"J. Morphol."},{"key":"10.1016\/j.anireprosci.2023.107253_bib41","doi-asserted-by":"crossref","first-page":"3993","DOI":"10.1007\/s11033-010-0517-0","article-title":"The role of actin and myosin during spermatogenesis","volume":"38","author":"Sun","year":"2011","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.anireprosci.2023.107253_bib42","doi-asserted-by":"crossref","first-page":"2510","DOI":"10.1111\/evo.13323","article-title":"Immigrant reproductive dysfunction facilitates ecological speciation","volume":"71","author":"Svensson","year":"2017","journal-title":"Evolution"},{"key":"10.1016\/j.anireprosci.2023.107253_bib43","doi-asserted-by":"crossref","DOI":"10.1016\/j.ygcen.2021.113794","article-title":"Associations between plasma testosterone levels and season, nutritional status, age, and body size in free-ranging male Asian black bears (Ursus thibetanus) in central Honshu, Japan","volume":"309","author":"Tomiyasu","year":"2021","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.anireprosci.2023.107253_bib44","unstructured":"Tracking Red-Eared Sliders, 2019, iNaturalist Project. https:\/\/www.inaturalist.org\/projects\/tracking-invasive-red-eared-sliders."},{"key":"10.1016\/j.anireprosci.2023.107253_bib45","doi-asserted-by":"crossref","first-page":"190","DOI":"10.3724\/SP.J.1245.2013.000190","article-title":"Interspecific differences in diet between introduced Red-eared sliders and native turtles in China","volume":"4","author":"Wang","year":"2013","journal-title":"Asian Herpetol. Res."},{"key":"10.1016\/j.anireprosci.2023.107253_bib46","doi-asserted-by":"crossref","DOI":"10.1111\/cpr.12933","article-title":"FGF9 promotes mouse spermatogonial stem cell proliferation mediated by p38 MAPK signalling","volume":"54","author":"Yang","year":"2021","journal-title":"Cell Prolif."},{"key":"10.1016\/j.anireprosci.2023.107253_bib47","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1002\/ar.20587","article-title":"Spermiogenesis in soft-shelled turtle, Pelodiscus sinensis","volume":"290","author":"Zhang","year":"2007","journal-title":"Anat. Rec. (Hoboken)"},{"key":"10.1016\/j.anireprosci.2023.107253_bib48","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.1016\/j.theriogenology.2008.01.028","article-title":"Seasonal effects on apoptosis and proliferation of germ cells in the testes of the Chinese soft-shelled turtle, Pelodiscus sinensis","volume":"69","author":"Zhang","year":"2008","journal-title":"Theriogenology"}],"container-title":["Animal Reproduction Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378432023000672?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378432023000672?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T06:49:11Z","timestamp":1759906151000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378432023000672"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6]]},"references-count":48,"alternative-id":["S0378432023000672"],"URL":"https:\/\/doi.org\/10.1016\/j.anireprosci.2023.107253","relation":{},"ISSN":["0378-4320"],"issn-type":[{"value":"0378-4320","type":"print"}],"subject":[],"published":{"date-parts":[[2023,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Seasonal spermatogenesis in the red-eared slider (Trachemys scripta elegans): The roles of GnRH, actin cytoskeleton, and MAPK","name":"articletitle","label":"Article Title"},{"value":"Animal Reproduction Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.anireprosci.2023.107253","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"107253"}}