{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T15:20:50Z","timestamp":1792682450482,"version":"4.5.0"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100005992","name":"Ministry of Education and Science","doi-asserted-by":"publisher","award":["\\u041401-62\/18.03.2021"],"award-info":[{"award-number":["\\u041401-62\/18.03.2021"]}],"id":[{"id":"10.13039\/501100005992","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Animal Reproduction Science"],"published-print":{"date-parts":[[2024,4]]},"DOI":"10.1016\/j.anireprosci.2024.107450","type":"journal-article","created":{"date-parts":[[2024,3,2]],"date-time":"2024-03-02T02:12:44Z","timestamp":1709345564000},"page":"107450","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["In vitro characteristics of X- and Y-bearing ram spermatozoa sorted by bovine serum albumin (BSA) column and TLR7\/8 ligand R848"],"prefix":"10.1016","volume":"263","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9435-6854","authenticated-orcid":false,"given":"Stanimir","family":"Yotov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7869-2022","authenticated-orcid":false,"given":"Desislava","family":"Abadjieva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anatoli","family":"Atanasov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8934-9609","authenticated-orcid":false,"given":"Boyana","family":"Ivanova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulina","family":"Taushanova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9481-1688","authenticated-orcid":false,"given":"Ivan","family":"Fasulkov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5239-1715","authenticated-orcid":false,"given":"Elena","family":"Kistanova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.anireprosci.2024.107450_bib1","unstructured":"Abadjieva, D., Kistanova, E., Yotov, S., Atanasov, A., Ivanova, B., Yarkov, D., Sinapov, B., 2022. Sexing of fresh ram semen by activation of the toll-like receptors 7\/8 (TLR7\/8). Reprod. Dom. Anim. 57, S4, 72. Proceedings of the 25th Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR) 28. September - 2. October 2022, Thessaloniki, Greece. \u3008https:\/\/www.webofscience.com\/wos\/woscc\/full-record\/WOS:000860438400093\u3009."},{"key":"10.1016\/j.anireprosci.2024.107450_bib2","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1111\/j.1365-2605.1988.tb00988.x","article-title":"Glutathione, L-glutamic acid and gamma-glutamyltransferase in the bull reproductive tissue","volume":"11","author":"Agrawal","year":"1988","journal-title":"Int. J. Androl."},{"issue":"4","key":"10.1016\/j.anireprosci.2024.107450_bib3","doi-asserted-by":"crossref","first-page":"437","DOI":"10.2298\/BAH2004437A","article-title":"Study of the relationship between the age of the rams and the quality of their ejaculates obtained outside the breeding season","volume":"36","author":"Andreeva","year":"2020","journal-title":"Biotechnol. Anim. Husb."},{"issue":"1","key":"10.1016\/j.anireprosci.2024.107450_bib4","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1111\/j.2047-2927.2013.00159.x","article-title":"Alkaline phosphatase in boar sperm function","volume":"2","author":"Bucci","year":"2014","journal-title":"Andrology"},{"key":"10.1016\/j.anireprosci.2024.107450_bib5","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1016\/0093-691X(92)90182-Q","article-title":"Low stability of aspartate aminotransferase activity in boar semen","volume":"37","author":"Ciereszko","year":"1992","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2024.107450_bib6","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1016\/j.theriogenology.2005.09.009","article-title":"Flow cytometric sexing of mammalian sperm","volume":"6","author":"Garner","year":"2006","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2024.107450_bib7","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.theriogenology.2020.12.028","article-title":"Small ruminant SexedULTRA\u2122 sperm sex-sorting: status report and recent developments","volume":"162","author":"Gonzalez-Mar\u00edn","year":"2021","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2024.107450_bib8","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.theriogenology.2008.09.014","article-title":"Sperm sexing in sheep and cattle: the exception and the rule","volume":"71","author":"de Graaf","year":"2009","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2024.107450_bib9","first-page":"144","article-title":"Separation of y-chromosome bearing ram\u2019s sperms using an albumin gradient technique and identification of embryos by PCR","volume":"12","author":"Hadi","year":"2013","journal-title":"AL-Qadisiyah J. Vet. Med. Sci."},{"key":"10.1016\/j.anireprosci.2024.107450_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.theriogenology.2023.02.012","article-title":"Diluent pH affects sperm motility via GSK3 \u03b1\/\u03b2-hexokinase pathway for the efficient enrichment of X-sperm to increase the female kids rate of dairy goats","volume":"201","author":"He","year":"2023","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2024.107450_bib11","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1071\/RD03060","article-title":"In vitro and in vivo assessment of functional capacity of flow cytometrically sorted ram spermatozoa after freezing and thawing","volume":"15","author":"Hollinshead","year":"2003","journal-title":"Reprod. Fertil. Dev."},{"key":"10.1016\/j.anireprosci.2024.107450_bib12","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0378-4320(00)00088-9","article-title":"Sexing mammalian sperm for production of offspring: the state of- the-art","volume":"61","author":"Johnson","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.anireprosci.2024.107450_bib13","doi-asserted-by":"crossref","first-page":"536","DOI":"10.2164\/jandrol.110.012583","article-title":"Seminal plasma: an essential attribute to spermatozoa","volume":"33","author":"Juyena","year":"2012","journal-title":"J. Androl."},{"key":"10.1016\/j.anireprosci.2024.107450_bib14","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1111\/j.1439-0531.2012.02077.x","article-title":"Seminal plasma and its effect on ruminant spermatozoa during processing","volume":"47","author":"Leahy","year":"2012","journal-title":"Reprod. Dom. Anim."},{"issue":"6","key":"10.1016\/j.anireprosci.2024.107450_bib15","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1530\/REP-18-0627","article-title":"Ram seminal plasma and its functional proteomic assessment","volume":"157","author":"Leahy","year":"2019","journal-title":"Reproduction"},{"key":"10.1016\/j.anireprosci.2024.107450_bib16","doi-asserted-by":"crossref","first-page":"5","DOI":"10.3109\/19396368.2012.725120","article-title":"Boar seminal plasma components and their relation with semen quality","volume":"59","author":"L\u00f3pez Rodr\u00edguez","year":"2013","journal-title":"Syst. Biol. Reprod. Med."},{"key":"10.1016\/j.anireprosci.2024.107450_bib17","doi-asserted-by":"crossref","first-page":"181","DOI":"10.3109\/10520295.2012.757366","article-title":"Quantification and identification of sperm sub-populations using computer-aided sperm analysis and species-specific cut-off values for swimming speed","volume":"3-4","author":"Maree","year":"2013","journal-title":"Biotech. Histochem."},{"key":"10.1016\/j.anireprosci.2024.107450_bib18","doi-asserted-by":"crossref","first-page":"1446","DOI":"10.1016\/j.theriogenology.2007.03.002","article-title":"Seasonal variations in antioxidant enzyme activity in ram seminal plasma","volume":"67","author":"Marti","year":"2007","journal-title":"Theriogenology"},{"key":"10.1016\/j.anireprosci.2024.107450_bib19","doi-asserted-by":"crossref","DOI":"10.1038\/srep17643","article-title":"Lactic acid is a sperm motility inactivation factor in the sperm storage tubules","volume":"5","author":"Matsuzaki","year":"2015","journal-title":"Sci. Rep."},{"issue":"9","key":"10.1016\/j.anireprosci.2024.107450_bib20","article-title":"Semen creatine and creatine kinase activity as an indicator of sperm quality","volume":"66","author":"Nasrallah","year":"2020","journal-title":"Clin. Lab."},{"issue":"1","key":"10.1016\/j.anireprosci.2024.107450_bib21","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.bbagen.2005.07.012","article-title":"Acrosome reaction in bovine spermatozoa: Role of reactive oxygen species and lactate dehydrogenase C4","volume":"1726","author":"O\u2019Flaherty","year":"2005","journal-title":"BBA - Gen. Subj."},{"issue":"4","key":"10.1016\/j.anireprosci.2024.107450_bib22","doi-asserted-by":"crossref","first-page":"315","DOI":"10.5487\/TR.2017.33.4.315","article-title":"Environmental exposure of sperm sex-chromosomes: a gender selection technique","volume":"33","author":"Oyeyipo","year":"2017","journal-title":"Toxicol. Res."},{"issue":"1-3","key":"10.1016\/j.anireprosci.2024.107450_bib23","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.smallrumres.2012.12.014","article-title":"Standardization of CASA sperm motility assessment in the ram","volume":"112","author":"Palac\u00edn","year":"2013","journal-title":"Small Rumin. Res."},{"key":"10.1016\/j.anireprosci.2024.107450_bib24","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1080\/1828051X.2017.1290509","article-title":"Influence of \u03b3-glutamyltransferase and alkaline phosphatase activity on in vitro fertilization of bovine frozen\/thawed semen","volume":"16","author":"Pero","year":"2017","journal-title":"Ital. J. Anim. Sci."},{"issue":"1","key":"10.1016\/j.anireprosci.2024.107450_bib25","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1186\/s40104-021-00613-y","article-title":"TLR7\/8 signalling affects X-sperm motility via the GSK3 \u03b1\/\u03b2-hexokinase pathway for the efficient production of sexed dairy goat embryos","volume":"12","author":"Ren","year":"2021","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"10.1016\/j.anireprosci.2024.107450_bib26","first-page":"113","article-title":"Quality and longevity of local ram\u2019s sexed sperm with albumin column","volume":"19","author":"Solihati","year":"2019","journal-title":"J. Ilmu Tern."},{"key":"10.1016\/j.anireprosci.2024.107450_bib27","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/S0006-291X(75)80142-2","article-title":"Phosphoprotein phosphatase of bovine epididymal spermatozoa","volume":"62","author":"Tang","year":"1975","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.anireprosci.2024.107450_bib28","doi-asserted-by":"crossref","first-page":"27","DOI":"10.5958\/2277-3371.2016.00003.6","article-title":"Enzymatic activities in fresh seminal plasma and extended refrigerated semen in Nari Suvarna rams","volume":"6","author":"Tejaswi","year":"2016","journal-title":"Theriogenol. Ins."},{"issue":"8","key":"10.1016\/j.anireprosci.2024.107450_bib29","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.3000398","article-title":"Activation of toll-like receptor 7\/8 encoded by the X chromosome alters sperm motility and provides a novel simple technology for sexing sperm","volume":"17","author":"Umehara","year":"2019","journal-title":"Plos Biol."},{"key":"10.1016\/j.anireprosci.2024.107450_bib30","doi-asserted-by":"crossref","first-page":"2645","DOI":"10.1038\/s41596-020-0348-y","article-title":"A simple sperm-sexing method that activates TLR7\/8 on X sperm for the efficient production of sexed mouse or cattle embryos","volume":"15","author":"Umehara","year":"2020","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.anireprosci.2024.107450_bib31","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/0378-4320(95)01442-X","article-title":"Enzyme leakage during cryopreservation of ram spermatozoa","volume":"41","author":"Upreti","year":"1996","journal-title":"Anim. Reprod. Sci."},{"issue":"1-2","key":"10.1016\/j.anireprosci.2024.107450_bib32","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.anireprosci.2014.02.005","article-title":"In vitro assessment of sperm quality from rams of high and low field fertility","volume":"46","author":"Vicente-Fiel","year":"2014","journal-title":"Anim. Reprod. Sci."},{"issue":"2","key":"10.1016\/j.anireprosci.2024.107450_bib33","first-page":"37","article-title":"Comparative analysis of motility characteristics and kinematic parameters of fresh, chilled and sexed ram semen - preliminary study","volume":"79","author":"Yotov","year":"2021","journal-title":"Vet. Zootech."}],"container-title":["Animal Reproduction Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378432024000411?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378432024000411?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T22:53:43Z","timestamp":1778540023000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378432024000411"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":33,"alternative-id":["S0378432024000411"],"URL":"https:\/\/doi.org\/10.1016\/j.anireprosci.2024.107450","relation":{},"ISSN":["0378-4320"],"issn-type":[{"value":"0378-4320","type":"print"}],"subject":[],"published":{"date-parts":[[2024,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"In vitro characteristics of X- and Y-bearing ram spermatozoa sorted by bovine serum albumin (BSA) column and TLR7\/8 ligand R848","name":"articletitle","label":"Article Title"},{"value":"Animal Reproduction Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.anireprosci.2024.107450","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"107450"}}