{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T03:56:42Z","timestamp":1793159802755,"version":"4.5.0"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Annals of Diagnostic Pathology"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.anndiagpath.2025.152500","type":"journal-article","created":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T15:49:12Z","timestamp":1747151352000},"page":"152500","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Rethinking tissue preservation: A review of non-toxic and environmentally sustainable fixatives"],"prefix":"10.1016","volume":"78","author":[{"given":"Sowmiya Shri","family":"Thangamani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swaathi","family":"Ravindran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magesh","family":"Karuppur Thiagarajan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sathya Kumar","family":"Mayilvakanam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aravindhan","family":"Ravi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sivachandran","family":"Annamalai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.anndiagpath.2025.152500_bb0005","series-title":"Basic and advanced laboratory techniques in histopathology and cytology","first-page":"3","article-title":"Fixation of histology samples: principles, methods and types of fixatives","author":"Dey","year":"2022"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0010","doi-asserted-by":"crossref","first-page":"113","DOI":"10.33545\/pathol.2021.v4.i4b.433","article-title":"Fixation and different types of fixatives: their role and functions: a review","volume":"4","author":"Bhat","year":"2021","journal-title":"International Journal of Clinical and Diagnostic Pathology"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0015","first-page":"183","article-title":"Tissue fixatives: a review","volume":"4","author":"Rai","year":"2016","journal-title":"International Journal of Pharmaceutics and Drug Analysis"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0020","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1093\/toxsci\/kfq061","article-title":"Distribution of DNA adducts caused by inhaled formaldehyde is consistent with induction of nasal carcinoma but not leukemia","volume":"116","author":"Lu","year":"2010","journal-title":"Toxicol Sci"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0025","unstructured":"Report to Congress on Indoor Air Quality Indoor Air Poll-ution Research Needs Statement Issued under Section 403(e), Title IV of the Superfund Amendments and Reauthorization Act. of-1986 (SARA)\u00a0:\\). 1989."},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0030","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/1476-069X-11-59","article-title":"Evaluation of two commercial and three home-made fixatives for the substitution of formalin: a formaldehyde\u2013free laboratory is possible","volume":"11","author":"Zanini","year":"2012","journal-title":"Environ Health"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0035","first-page":"22290","article-title":"Occupational exposure to formaldehyde\u2013OSHA: response to court remand; final rule","year":"1992","journal-title":"Fed Regist"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0040","series-title":"IARC monographs on the evaluation of carcinogenic risks to humans","article-title":"Wood dust and formaldehyde","volume":"62","year":"1995"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0045","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1055\/s-0040-1722546","article-title":"Histomorphological assessment of formalin versus nonformalin fixatives in diagnostic surgical pathology","volume":"12","author":"Shetty","year":"2020","journal-title":"J Lab Physicians"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0050","doi-asserted-by":"crossref","first-page":"2237","DOI":"10.3390\/polym12102237","article-title":"Bio-based alternatives to phenol and formaldehyde for the production of resins","volume":"12","author":"Sarika","year":"2020","journal-title":"Polymers (Basel)"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0055","first-page":"31","article-title":"Revelation in the field of tissue preservation - a preliminary study on natural formalin substitutes","volume":"5","author":"Patil","year":"2013","journal-title":"J Int Oral Health"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0060","doi-asserted-by":"crossref","first-page":"67","DOI":"10.4103\/0976-9668.149089","article-title":"Natural sweeteners as fixatives in histopathology: a longitudinal study","volume":"6","author":"Patil","year":"2015","journal-title":"J Nat Sci Biol Med"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0065","doi-asserted-by":"crossref","first-page":"458","DOI":"10.4103\/jomfp.JOMFP_236_15","article-title":"Comparitive efficacies of a natural fixative with a conventional fixative","volume":"21","author":"Sinha","year":"2017","journal-title":"Journal of Oral and Maxillofacial Pathology"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0070","doi-asserted-by":"crossref","first-page":"14","DOI":"10.4103\/njms.NJMS_57_17","article-title":"Probing natural substitute for formalin: comparing honey, sugar, and jaggery syrup as fixatives","volume":"9","author":"Bhattacharyya","year":"2018","journal-title":"Natl J Maxillofac Surg"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0075","doi-asserted-by":"crossref","DOI":"10.7759\/cureus.28149","article-title":"Honey as a cytological fixative: a comparative study with 95% alcohol","author":"Priyadarshi","year":"2022","journal-title":"Cureus"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0080","doi-asserted-by":"crossref","first-page":"150","DOI":"10.4103\/joc.joc_182_23","article-title":"Comparative evaluation of honey versus alcohol as a fixative in cytological samples: an institutional study","volume":"41","author":"Priyanka","year":"2024","journal-title":"J Cytol"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0085","doi-asserted-by":"crossref","DOI":"10.4103\/drj.drj_88_23","article-title":"Standardization of honey as a tissue fixative for histopathology: a morphometric study","volume":"21","author":"Sabarinath","year":"2024","journal-title":"Dent Res J (Isfahan)"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0090","doi-asserted-by":"crossref","first-page":"143","DOI":"10.4103\/jomfp.JOMFP_53_16","article-title":"Natural substitutes for formalin: a boon to histopathology!!","volume":"22","author":"Chittemsetti","year":"2018","journal-title":"Journal of Oral and Maxillofacial Pathology"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0095","doi-asserted-by":"crossref","first-page":"33","DOI":"10.4103\/jomfp.jomfp_489_20","article-title":"Qualitative assessment of Aloe vera as natural tissue fixative","volume":"27","author":"Narwal","year":"2023","journal-title":"Journal of Oral and Maxillofacial Pathology"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0100","doi-asserted-by":"crossref","first-page":"238","DOI":"10.4103\/ijpm.ijpm_510_23","article-title":"Sirka: an alternative to the formalin fixative","volume":"67","author":"Gotur","year":"2024","journal-title":"Indian J Pathol Microbiol"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0105","doi-asserted-by":"crossref","first-page":"286","DOI":"10.4103\/ijdr.IJDR_246_16","article-title":"Honey and olive oil as bio-friendly substitutes for formalin and xylene in routine histopathology","volume":"28","author":"Muddana","year":"2017","journal-title":"Indian J Dent Res"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0110","series-title":"Toxicological profile for formaldehyde","author":"Agency for Toxic Substances and Disease Registry (ATSDR)","year":"1999"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0115","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1089\/thy.2009.0095","article-title":"Assessment of morphology, antigenicity, and nucleic acid integrity for diagnostic thyroid pathology using formalin substitute fixatives","volume":"19","author":"Lassalle","year":"2009","journal-title":"Thyroid"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0120","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1179\/his.2009.32.2.54","article-title":"Immunocytochemical evaluation of ductal carcinoma in breast after preservation in honey","volume":"32","author":"Gunter","year":"2009","journal-title":"J Histotechnol"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0125","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2174\/2210290601102010001","article-title":"Antitumor activity of *Dendrophthoe falcata* against Ehrlich ascites carcinoma in Swiss albino mice","volume":"2","author":"Dashora","year":"2011","journal-title":"Pharm Crop"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0130","doi-asserted-by":"crossref","first-page":"113","DOI":"10.4103\/0970-9371.160563","article-title":"Comparison of honey with ethanol as an oral cytological fixative: a pilot study","volume":"32","author":"Singh","year":"2015","journal-title":"J Cytol"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0135","doi-asserted-by":"crossref","first-page":"6","DOI":"10.3390\/jfb8010006","article-title":"Aloe Vera for tissue engineering applications","volume":"8","author":"Rahman","year":"2017","journal-title":"J Funct Biomater"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0140","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/0926-6569(63)90108-1","article-title":"The identification of inhibine, the antibacterial factor in honey, as hydrogen peroxide and its origin in a honey glucose-oxidase system","volume":"73","author":"White","year":"1963","journal-title":"Biochim Biophys Acta"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0145","first-page":"121","article-title":"Honey and health: a review of recent clinical research","volume":"9","author":"Samarghandian","year":"2017","journal-title":"Pharm Res"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0150","doi-asserted-by":"crossref","first-page":"342","DOI":"10.4103\/0973-029X.174641","article-title":"Honey as an alternative fixative for oral tissue: an evaluation of processed and unprocessed honey","volume":"19","author":"Lalwani","year":"2015","journal-title":"J Oral Maxillofac Pathol"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0155","doi-asserted-by":"crossref","first-page":"113792","DOI":"10.1016\/j.jenvman.2021.113792","article-title":"Technological advancements in jaggery-making processes and emission reduction potential via clean combustion for sustainable jaggery production: an overview","volume":"301","author":"Tyagi","year":"2022","journal-title":"J Environ Manag"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0160","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodchem.2019.125094","article-title":"Effects of acid treatment in jaggery making","volume":"299","author":"Verma","year":"2019","journal-title":"Food Chem"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0165","first-page":"95","article-title":"Jaggery - a traditional Indian sweetener","volume":"6","author":"PDas","year":"2007","journal-title":"Indian J Tradit Knowl"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0170","series-title":"Responsible care","first-page":"383","article-title":"CHAPTER SIX - The food and dairy industry","author":"Cheremisinoff","year":"2008"},{"key":"10.1016\/j.anndiagpath.2025.152500_bb0175","article-title":"Vinegar: a potential source of healthy and functional food with special reference to sugarcane vinegar","volume":"10","author":"Chen","year":"2023","journal-title":"Front Nutr"}],"container-title":["Annals of Diagnostic Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1092913425000656?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1092913425000656?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,6,7]],"date-time":"2025-06-07T02:14:47Z","timestamp":1749262487000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1092913425000656"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":35,"alternative-id":["S1092913425000656"],"URL":"https:\/\/doi.org\/10.1016\/j.anndiagpath.2025.152500","relation":{},"ISSN":["1092-9134"],"issn-type":[{"value":"1092-9134","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Rethinking tissue preservation: A review of non-toxic and environmentally sustainable fixatives","name":"articletitle","label":"Article Title"},{"value":"Annals of Diagnostic Pathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.anndiagpath.2025.152500","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"152500"}}