{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T16:14:22Z","timestamp":1788970462883,"version":"build-2803163510"},"reference-count":25,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:00:00Z","timestamp":1782950400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25K10294"],"award-info":[{"award-number":["25K10294"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Annals of Diagnostic Pathology"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.anndiagpath.2026.152681","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T07:04:46Z","timestamp":1782975886000},"page":"152681","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Determinants of nucleic acid quality in formalin-fixed paraffin-embedded bladder urothelial carcinoma specimens: effects of specimen type, fixation conditions, and storage time"],"prefix":"10.1016","volume":"85","author":[{"given":"Shu","family":"Yuguchi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7079-4454","authenticated-orcid":false,"given":"Yoichiro","family":"Okubo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rika","family":"Kasajima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noboru","family":"Nakaigawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukihiko","family":"Hiroshima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chie","family":"Hasegawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kota","family":"Washimi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinya","family":"Sato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoyuki","family":"Yokose","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuma","family":"Juichi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuto","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimitsugu","family":"Usui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohei","family":"Miyagi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.anndiagpath.2026.152681_bb0005","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1111\/pin.13170","article-title":"The Japanese Society of Pathology Practical Guidelines on the handling of pathological tissue samples for cancer genomic medicine","volume":"71","author":"Hatanaka","year":"2021","journal-title":"Pathol Int"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0010","doi-asserted-by":"crossref","DOI":"10.1155\/2017\/1926304","article-title":"The utilization of formalin-fixed paraffin-embedded specimens in high-throughput genomic studies","volume":"2017","author":"Zhang","year":"2017","journal-title":"Int J Genomics"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0015","doi-asserted-by":"crossref","first-page":"7143","DOI":"10.1093\/nar\/gkad519","article-title":"A critical spotlight on the paradigms of FFPE-DNA sequencing","volume":"51","author":"Steiert","year":"2023","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0020","doi-asserted-by":"crossref","DOI":"10.3390\/genes12101472","article-title":"Formalin-fixed and paraffin-embedded samples for next generation sequencing: problems and solutions","volume":"12","author":"Cazzato","year":"2021","journal-title":"Genes"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0025","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0203608","article-title":"Impact of storage conditions on the quality of nucleic acids in paraffin embedded tissues","volume":"13","author":"Groelz","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0030","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1007\/s00428-010-0917-5","article-title":"Multicentre validation study of nucleic acids extraction from FFPE tissues","volume":"457","author":"Bonin","year":"2010","journal-title":"Virchows Arch"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0035","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1007\/s12022-024-09838-9","article-title":"Effective preparation of FFPE tissue samples for preserving appropriate nucleic acid quality for genomic analysis in thyroid carcinoma","volume":"35","author":"Okubo","year":"2024","journal-title":"Endocr Pathol"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0040","doi-asserted-by":"crossref","DOI":"10.1155\/2020\/9349132","article-title":"DV200 index for assessing RNA integrity in next-generation sequencing","volume":"2020","author":"Matsubara","year":"2020","journal-title":"Biomed Res Int"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0045","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2199-7-3","article-title":"The RIN: an RNA integrity number for assigning integrity values to RNA measurements","volume":"7","author":"Schroeder","year":"2006","journal-title":"BMC Mol Biol"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0050","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1093\/toxsci\/kfz113","article-title":"Enhanced quality metrics for assessing RNA derived from archival formalin-fixed paraffin-embedded tissue samples","volume":"170","author":"Wehmas","year":"2019","journal-title":"Toxicol Sci"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0055","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.eururo.2019.09.006","article-title":"A consensus molecular classification of muscle-invasive bladder cancer","volume":"77","author":"Kamoun","year":"2020","journal-title":"Eur Urol"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0060","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/s13073-019-0669-z","article-title":"Long non-coding RNAs identify a subset of luminal muscle-invasive bladder cancer patients with favorable prognosis","volume":"11","author":"de Jong","year":"2019","journal-title":"Genome Med"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0065","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/0090-4295(75)90639-1","article-title":"Cautery artifact in bladder biopsy: histopathologic evaluation","volume":"6","author":"Tannenbaum","year":"1975","journal-title":"Urology"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0070","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1089\/end.2004.18.578","article-title":"Use of bipolar energy for transurethral resection of bladder tumors: pathologic considerations","volume":"18","author":"Wang","year":"2004","journal-title":"J Endourol"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0075","doi-asserted-by":"crossref","first-page":"379","DOI":"10.4111\/kju.2011.52.6.379","article-title":"Comparison of deep biopsy tissue damage from transurethral resection of bladder tumors between bipolar and monopolar devices","volume":"52","author":"Yang","year":"2011","journal-title":"Korean J Urol"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0080","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1016\/j.juro.2013.12.004","article-title":"Monopolar versus bipolar transurethral resection of bladder tumors: a single center, parallel arm, randomized, controlled trial","volume":"191","author":"Venkatramani","year":"2014","journal-title":"J Urol"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0085","doi-asserted-by":"crossref","first-page":"E203","DOI":"10.5489\/cuaj.4172","article-title":"Cautery artifact understages urothelial cancer at initial transurethral resection of large bladder tumours","volume":"11","author":"Truong","year":"2017","journal-title":"Can Urol Assoc J"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0090","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/j.jamcollsurg.2005.11.017","article-title":"Electrosurgery: history, principles, and current and future uses","volume":"202","author":"Massarweh","year":"2006","journal-title":"J Am Coll Surg"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0095","doi-asserted-by":"crossref","first-page":"5364","DOI":"10.1021\/ja990592p","article-title":"Kinetics of RNA degradation by specific base catalysis of transesterification involving the 2\u2032-hydroxyl group","volume":"121","author":"Li","year":"1999","journal-title":"J Am Chem Soc"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0100","doi-asserted-by":"crossref","first-page":"803","DOI":"10.3762\/bjoc.14.68","article-title":"Phosphodiester models for cleavage of nucleic acids","volume":"14","author":"Mikkola","year":"2018","journal-title":"Beilstein J Org Chem"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0105","doi-asserted-by":"crossref","first-page":"5432","DOI":"10.1021\/bi00367a014","article-title":"Why does ribonuclease irreversibly inactivate at high temperatures?","volume":"25","author":"Zale","year":"1986","journal-title":"Biochemistry"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0110","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1056\/NEJMoa022148","article-title":"Neoadjuvant chemotherapy plus cystectomy compared with cystectomy alone for locally advanced bladder cancer","volume":"349","author":"Grossman","year":"2003","journal-title":"N Engl J Med"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0115","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1093\/hmg\/dds486","article-title":"Oncogenic FGFR3 gene fusions in bladder cancer","volume":"22","author":"Williams","year":"2013","journal-title":"Hum Mol Genet"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0120","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1016\/j.euf.2020.12.003","article-title":"Holmium laser en-bloc resection versus conventional transurethral resection of bladder tumors for treatment of non-muscle-invasive bladder cancer: a randomized clinical trial","volume":"7","author":"Hashem","year":"2021","journal-title":"Eur Urol Focus"},{"key":"10.1016\/j.anndiagpath.2026.152681_bb0125","doi-asserted-by":"crossref","DOI":"10.1016\/j.anndiagpath.2025.152496","article-title":"Effect of storage temperature on nucleic acid quality in formalin-fixed paraffin-embedded tissue samples","volume":"78","author":"Okubo","year":"2025","journal-title":"Ann Diagn Pathol"}],"container-title":["Annals of Diagnostic Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1092913426000778?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1092913426000778?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T15:32:49Z","timestamp":1788967969000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1092913426000778"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":25,"alternative-id":["S1092913426000778"],"URL":"https:\/\/doi.org\/10.1016\/j.anndiagpath.2026.152681","relation":{},"ISSN":["1092-9134"],"issn-type":[{"value":"1092-9134","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Determinants of nucleic acid quality in formalin-fixed paraffin-embedded bladder urothelial carcinoma specimens: effects of specimen type, fixation conditions, and storage time","name":"articletitle","label":"Article Title"},{"value":"Annals of Diagnostic Pathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.anndiagpath.2026.152681","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"152681"}}