{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T03:01:08Z","timestamp":1780628468490,"version":"3.54.1"},"reference-count":36,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"NIH\/NIAID","award":["AI066160"],"award-info":[{"award-number":["AI066160"]}]},{"DOI":"10.13039\/100000060","name":"NIAID","doi-asserted-by":"crossref","award":["1 T32 AI065396-01"],"award-info":[{"award-number":["1 T32 AI065396-01"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Library of Medicine Training Program","award":["T15 LM007093"],"award-info":[{"award-number":["T15 LM007093"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Antiviral Research"],"published-print":{"date-parts":[[2012,2]]},"DOI":"10.1016\/j.antiviral.2011.12.003","type":"journal-article","created":{"date-parts":[[2011,12,13]],"date-time":"2011-12-13T12:48:49Z","timestamp":1323780529000},"page":"245-252","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":42,"title":["Use of parallel validation high-throughput screens to reduce false positives and identify novel dengue NS2B-NS3 protease inhibitors"],"prefix":"10.1016","volume":"93","author":[{"given":"Suzanne M.","family":"Tomlinson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanley J.","family":"Watowich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.antiviral.2011.12.003_b0005","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1475-2883-5-11","article-title":"The Mectizan donation program \u2013 highlights from 2005","volume":"5","author":"Alleman","year":"2006","journal-title":"Filaria J."},{"key":"10.1016\/j.antiviral.2011.12.003_b0010","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.4269\/ajtmh.2006.75.1113","article-title":"Dengue hemorrhagic fever caused by sequential dengue 1\u20133 virus infections over a long time interval: Havana epidemic, 2001\u20132002","volume":"75","author":"Alvarez","year":"2006","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.antiviral.2011.12.003_b0015","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.1128\/jvi.66.3.1535-1542.1992","article-title":"Cleavage of the dengue virus polyprotein at the NS3\/NS4A and NS4B\/NS5 junctions are mediated by viral protease NS2B-NS3, whereas NS4A\/NS4B may be processed by a cellular protease","volume":"66","author":"Cahour","year":"1992","journal-title":"J. Virol."},{"key":"10.1016\/j.antiviral.2011.12.003_b0020","first-page":"577","article-title":"Locally acquired Dengue \u2013 Key West Florida 2009\u20132010","volume":"59","author":"CDC","year":"2010","journal-title":"MMWR Morb. Mortal Weekly Rep."},{"key":"10.1016\/j.antiviral.2011.12.003_b0025","doi-asserted-by":"crossref","first-page":"6797","DOI":"10.1128\/jvi.67.11.6797-6807.1993","article-title":"Mutagenesis of the yellow fever virus NS2B protein: effects on proteolytic processing, NS2B-NS3 complex formation, and viral replication","volume":"67","author":"Chambers","year":"1993","journal-title":"J. Virol."},{"key":"10.1016\/j.antiviral.2011.12.003_b0030","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1016\/j.bbrc.2005.03.107","article-title":"Competitive inhibition of the dengue virus NS3 serine protease by synthetic peptides representing polyprotein cleavage sites","volume":"330","author":"Chanprapaph","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.antiviral.2011.12.003_b0035","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1038\/nsmb1073","article-title":"Structural basis for the activation of flaviviral NS3 proteases from dengue and West Nile virus","volume":"13","author":"Erbel","year":"2006","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.antiviral.2011.12.003_b0040","doi-asserted-by":"crossref","first-page":"2467","DOI":"10.1128\/jvi.65.5.2467-2475.1991","article-title":"Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins","volume":"65","author":"Falgout","year":"1991","journal-title":"J. Virol."},{"key":"10.1016\/j.antiviral.2011.12.003_b0045","series-title":"Field\u2019s Virology","author":"Fields","year":"1996"},{"key":"10.1016\/j.antiviral.2011.12.003_b0050","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/S0065-3527(03)60011-4","article-title":"Neutralization and antibody-dependent enhancement of dengue viruses","volume":"60","author":"Halstead","year":"2003","journal-title":"Adv. Virus Res."},{"key":"10.1016\/j.antiviral.2011.12.003_b0055","doi-asserted-by":"crossref","first-page":"198","DOI":"10.33321\/cdi.2009.33.19","article-title":"Dengue in north Queensland, 2005\u20132008","volume":"33","author":"Hanna","year":"2009","journal-title":"Commun. Dis. Intell."},{"key":"10.1016\/j.antiviral.2011.12.003_b0060","doi-asserted-by":"crossref","first-page":"e381","DOI":"10.1371\/journal.pntd.0000381","article-title":"Is paromomycin an effective and safe treatment against cutaneous leishmaniasis? A meta-analysis of 14 randomized controlled trials","volume":"3","author":"Kim","year":"2009","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.antiviral.2011.12.003_b0065","doi-asserted-by":"crossref","first-page":"e693","DOI":"10.1016\/j.ijid.2009.12.010","article-title":"Epidemiological trends and the effect of airport fever screening on prevention of domestic dengue fever outbreaks in Taiwan, 1998\u20132007","volume":"14","author":"Kuan","year":"2010","journal-title":"Int. J. Infect. Dis."},{"key":"10.1016\/j.antiviral.2011.12.003_b0070","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1006\/abio.1996.0238","article-title":"Program DYNAFIT for the analysis of enzyme kinetic data: application to HIV proteinase","volume":"237","author":"Kuzmic","year":"1996","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.antiviral.2011.12.003_b0075","doi-asserted-by":"crossref","first-page":"19676","DOI":"10.2807\/ese.15.39.19676-en","article-title":"First two autochthonous dengue virus infections in metropolitan France, September 2010","volume":"15","author":"La Ruche","year":"2010","journal-title":"Euro. Surveill."},{"key":"10.1016\/j.antiviral.2011.12.003_b0080","doi-asserted-by":"crossref","first-page":"45762","DOI":"10.1074\/jbc.M107360200","article-title":"Activity of recombinant dengue 2 virus NS3 protease in the presence of a truncated NS2B co-factor, small peptide substrates, and inhibitors","volume":"276","author":"Leung","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.antiviral.2011.12.003_b0085","doi-asserted-by":"crossref","first-page":"28766","DOI":"10.1074\/jbc.M500588200","article-title":"Functional profiling of recombinant NS3 proteases from all four serotypes of dengue virus using tetrapeptide and octapeptide substrate libraries","volume":"280","author":"Li","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.antiviral.2011.12.003_b0090","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1128\/CMR.12.1.147","article-title":"Antiseptics and disinfectants: activity, action, and resistance","volume":"12","author":"McDonnell","year":"1999","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.antiviral.2011.12.003_b0095","doi-asserted-by":"crossref","first-page":"3821","DOI":"10.1007\/s00018-009-0129-9","article-title":"Gramicidin S and polymyxins: the revival of cationic cyclic peptide antibiotics","volume":"66","author":"Mogi","year":"2009","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.antiviral.2011.12.003_b0100","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1016\/j.jhazmat.2008.10.083","article-title":"In vitro genotoxic and cytotoxic effects of ivermectin and its formulation on Chinese hamster ovary (CHOK1) cells","volume":"165","author":"Molinari","year":"2009","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.antiviral.2011.12.003_b0105","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/j.biocel.2006.10.025","article-title":"Characterization of theWest Nile virus protease substrate specificity and inhibitors","volume":"39","author":"Mueller","year":"2007","journal-title":"Int. J. Biochem. Cell Biol."},{"issue":"Suppl 1","key":"10.1016\/j.antiviral.2011.12.003_b0110","first-page":"S173","article-title":"Mectizan procurement and delivery for onchocerciasis mass drug administration programmes","volume":"120","author":"Ogoussan","year":"2010","journal-title":"Acta Trop."},{"key":"10.1016\/j.antiviral.2011.12.003_b0115","doi-asserted-by":"crossref","first-page":"573","DOI":"10.7164\/antibiotics.41.573","article-title":"Screening for new nematocidal substances of microbial origin by a new method using the pine wood nematode","volume":"41","author":"Otoguro","year":"1988","journal-title":"J. Antibiot. (Tokyo)"},{"key":"10.1016\/j.antiviral.2011.12.003_b0120","first-page":"2","article-title":"Recent developments in the control of ectoparasites and endoparasites of dogs and cats","volume":"58","author":"Pipano","year":"2002","journal-title":"Israel J. Veter. Med."},{"key":"10.1016\/j.antiviral.2011.12.003_b0125","first-page":"327","article-title":"New approaches to structure-based discovery of dengue protease inhibitors","volume":"9","author":"Tomlinson","year":"2009","journal-title":"Infect. Disord.: Drug Targets"},{"key":"10.1016\/j.antiviral.2011.12.003_b0130","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.antiviral.2009.02.190","article-title":"Structure-based discovery of dengue virus protease inhibitors","volume":"82","author":"Tomlinson","year":"2009","journal-title":"Antiviral Res."},{"key":"10.1016\/j.antiviral.2011.12.003_b0135","doi-asserted-by":"crossref","first-page":"11763","DOI":"10.1021\/bi801034f","article-title":"Substrate inhibition kinetic model for West Nile virus NS2B-NS3 protease","volume":"47","author":"Tomlinson","year":"2008","journal-title":"Biochemistry"},{"key":"10.1016\/j.antiviral.2011.12.003_b0140","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.antiviral.2010.12.006","article-title":"Anthracene-based inhibitors of dengue virus NS2B-NS3 protease","volume":"89","author":"Tomlinson","year":"2011","journal-title":"Antiviral Res."},{"key":"10.1016\/j.antiviral.2011.12.003_b0145","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1177\/1087057110390360","article-title":"An Alpha Screen(R)-based assay for high-throughput screening for specific inhibitors of nuclear import","volume":"16","author":"Wagstaff","year":"2011","journal-title":"J. Biomol. Screen"},{"key":"10.1016\/j.antiviral.2011.12.003_b0150","unstructured":"WHO (2010).http:\/\/www.who.int\/mediacentre\/factsheets\/fs117\/en\/."},{"key":"10.1016\/j.antiviral.2011.12.003_b0155","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.tiv.2007.08.019","article-title":"A comparative study examining the cytotoxicity of inducible gene expression system ligands in different cell types","volume":"22","author":"Xie","year":"2008","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.antiviral.2011.12.003_b0160","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1128\/AAC.00855-10","article-title":"Novel dengue virus-specific NS2B\/NS3 protease inhibitor, BP2109, discovered by a high-throughput screening assay","volume":"55","author":"Yang","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.antiviral.2011.12.003_b0165","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1016\/j.ijpara.2004.04.010","article-title":"An ivermectin-sensitive glutamate-gated chloride channel subunit from Dirofilariaimmitis","volume":"34","author":"Yates","year":"2004","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.antiviral.2011.12.003_b0170","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.bmcl.2005.09.049","article-title":"Peptide inhibitors of dengue virus NS3 protease. Part 2: SAR study of tetrapeptide aldehyde inhibitors","volume":"16","author":"Yin","year":"2006","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"10.1016\/j.antiviral.2011.12.003_b0175","doi-asserted-by":"crossref","first-page":"2234","DOI":"10.1158\/1535-7163.MCT-06-0134","article-title":"Potential use of alexidinedihydrochloride as an apoptosis-promoting anticancer agent","volume":"5","author":"Yip","year":"2006","journal-title":"Mol. Cancer Ther."},{"key":"10.1016\/j.antiviral.2011.12.003_b0180","doi-asserted-by":"crossref","first-page":"9963","DOI":"10.1074\/jbc.275.14.9963","article-title":"Purified NS2B\/NS3 serine protease of dengue virus type 2 exhibits cofactor NS2B dependence for cleavage of substrates with dibasic amino acids in vitro","volume":"275","author":"Yusof","year":"2000","journal-title":"J. Biol. Chem."}],"container-title":["Antiviral Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166354211005225?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166354211005225?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T12:25:32Z","timestamp":1759062332000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166354211005225"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2]]},"references-count":36,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,2]]}},"alternative-id":["S0166354211005225"],"URL":"https:\/\/doi.org\/10.1016\/j.antiviral.2011.12.003","relation":{},"ISSN":["0166-3542"],"issn-type":[{"value":"0166-3542","type":"print"}],"subject":[],"published":{"date-parts":[[2012,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Use of parallel validation high-throughput screens to reduce false positives and identify novel dengue NS2B-NS3 protease inhibitors","name":"articletitle","label":"Article Title"},{"value":"Antiviral Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.antiviral.2011.12.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2011 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}