{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T10:36:58Z","timestamp":1778582218694,"version":"3.51.4"},"reference-count":32,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,8,16]],"date-time":"2016-08-16T00:00:00Z","timestamp":1471305600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Innovative Economy Program","award":["WND-POIG.01.01.02-00-007\/08"],"award-info":[{"award-number":["WND-POIG.01.01.02-00-007\/08"]}]},{"DOI":"10.13039\/501100011658","name":"FRISBI","doi-asserted-by":"crossref","award":["ANR-10-INSB-05-02"],"award-info":[{"award-number":["ANR-10-INSB-05-02"]}],"id":[{"id":"10.13039\/501100011658","id-type":"DOI","asserted-by":"crossref"}]},{"name":"GRAL","award":["ANR-10-LABX-49-01"],"award-info":[{"award-number":["ANR-10-LABX-49-01"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Antiviral Research"],"published-print":{"date-parts":[[2016,9]]},"DOI":"10.1016\/j.antiviral.2016.08.001","type":"journal-article","created":{"date-parts":[[2016,8,4]],"date-time":"2016-08-04T23:54:59Z","timestamp":1470354899000},"page":"242-249","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":23,"special_numbering":"C","title":["An avian influenza H5N1 virus vaccine candidate based on the extracellular domain produced in yeast system as subviral particles protects chickens from lethal challenge"],"prefix":"10.1016","volume":"133","author":[{"given":"Maria","family":"Pietrzak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Macio\u0142a","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konrad","family":"Zdanowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3200-7736","authenticated-orcid":false,"given":"Anna Maria","family":"Protas-Klukowska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Olszewska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krzysztof","family":"\u015amietanka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zenon","family":"Minta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bogus\u0142aw","family":"Szewczyk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edyta","family":"Kopera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.antiviral.2016.08.001_bib1","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1083\/jcb.103.4.1179","article-title":"Assembly of Influenza hemagglutinin trimers and its role in intracellular transport","volume":"103","author":"Copeland","year":"1986","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.antiviral.2016.08.001_bib2","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1038\/319199a0","article-title":"Solvation energy in protein folding and binding","volume":"319","author":"Eisenberg","year":"1986","journal-title":"Nature"},{"key":"10.1016\/j.antiviral.2016.08.001_bib3","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1099\/vir.0.83524-0","article-title":"Antibodies induced by the HA2 glycopolypeptide of influenza virus haemagglutinin improve recovery from influenza A virus infection","volume":"89","author":"Gocnik","year":"2008","journal-title":"J.\u00a0Gen. Virol."},{"key":"10.1016\/j.antiviral.2016.08.001_bib4","unstructured":"http:\/\/www.cbs.dtu.dk\/services\/NetNGlyc\/, http:\/\/www.usda\/avian_influenza.html."},{"key":"10.1016\/j.antiviral.2016.08.001_bib5","doi-asserted-by":"crossref","first-page":"597","DOI":"10.18388\/abp.2014_1882","article-title":"Expression, purification and characterization of glycosylated influenza H5N1 hemagglutinin produced in Pichia pastoris","volume":"61","author":"Kopera","year":"2014","journal-title":"Acta Biochim. Pol."},{"key":"10.1016\/j.antiviral.2016.08.001_bib6","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.vaccine.2008.07.025","article-title":"Pathology of human influenza revisited","volume":"26","author":"Kuiken","year":"2008","journal-title":"Vaccine"},{"key":"10.1016\/j.antiviral.2016.08.001_bib7","doi-asserted-by":"crossref","first-page":"12","DOI":"10.7774\/cevr.2014.3.1.12","article-title":"New vaccines against influenza virus","volume":"3","author":"Lee","year":"2014","journal-title":"Clin. Exp. Vaccine Res."},{"key":"10.1016\/j.antiviral.2016.08.001_bib8","doi-asserted-by":"crossref","first-page":"7953","DOI":"10.1128\/JVI.00449-10","article-title":"Insertion of a multibasic cleavage motif into the hemagglutinin of a low-pathogenic avian influenza H6N1 virus induces a highly pathogenic phenotype","volume":"84","author":"Munster","year":"2010","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.antiviral.2016.08.001_bib9","author":"OIE"},{"key":"10.1016\/j.antiviral.2016.08.001_bib10","doi-asserted-by":"crossref","first-page":"2573","DOI":"10.1073\/pnas.1200039109","article-title":"Hemagglutinin stalk antibodies elicited by the 2009 pandemic influenza virus as a mechanism for the extinction of seasonal H1N1 viruses","volume":"109","author":"Pica","year":"2012","journal-title":"Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.antiviral.2016.08.001_bib11","series-title":"Polish Law","year":"2005"},{"key":"10.1016\/j.antiviral.2016.08.001_bib12","doi-asserted-by":"crossref","first-page":"4975","DOI":"10.1016\/j.vaccine.2015.07.026","article-title":"Recombinant H7 hemagglutinin forms subviral particles that protect mice and ferrets from challenge with H7N9 influenza virus","volume":"33","author":"Pushko","year":"2015","journal-title":"Vaccine"},{"key":"10.1016\/j.antiviral.2016.08.001_bib13","doi-asserted-by":"crossref","first-page":"137","DOI":"10.20506\/rst.28.1.1876","article-title":"Avian influenza viruses in mammals","volume":"28","author":"Reperant","year":"2009","journal-title":"Rev. Sci. Tech."},{"key":"10.1016\/j.antiviral.2016.08.001_bib14","doi-asserted-by":"crossref","first-page":"3048","DOI":"10.1128\/JVI.67.6.3048-3060.1993","article-title":"Role of conserved glycosylation sites in maturation and transport of influenza A virus hemagglutinin","volume":"67","author":"Roberts","year":"1993","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.antiviral.2016.08.001_bib15","first-page":"374","article-title":"Monoclonal antibodies to glycopolypeptides HA1 and HA2 of influenza virus haemagglutinin","volume":"31","author":"Russ","year":"1987","journal-title":"Acta Virol."},{"key":"10.1016\/j.antiviral.2016.08.001_bib16","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1046\/j.1432-1327.1999.00150.x","article-title":"Protection of mice against a lethal influenza virus challenge after immunization with yeast-derived secreted influenza virus hemagglutinin","volume":"260","author":"Saelens","year":"1999","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.antiviral.2016.08.001_bib17","doi-asserted-by":"crossref","first-page":"425","DOI":"10.2307\/1592241","article-title":"Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential","volume":"40","author":"Senne","year":"1996","journal-title":"Avian Dis."},{"key":"10.1016\/j.antiviral.2016.08.001_bib18","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1016\/j.vaccine.2008.11.108","article-title":"Plant-derived hemagglutinin protects against challenge infection with the A\/Indonesia\/05\/05 strain of avian influenza","volume":"27","author":"Shoji","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.antiviral.2016.08.001_bib19","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.virol.2010.05.034","article-title":"Protective immunity against H5N1 influenza virus by a single dose vaccination with virus-like particles","volume":"405","author":"Song","year":"2010","journal-title":"Virology"},{"key":"10.1016\/j.antiviral.2016.08.001_bib20","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1177\/104063870802000512","article-title":"Analytical validation of a real-time reverse transcription polymerase chain reaction test for Pan-American lineage H7 subtype Avian influenza viruses","volume":"20","author":"Spackman","year":"2008","journal-title":"J.\u00a0Vet. Diagn. Invest."},{"key":"10.1016\/j.antiviral.2016.08.001_bib21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/viro.1999.9716","article-title":"Role of hemagglutinin cleavage for the pathogenicity of influenza virus","volume":"258","author":"Steinhauer","year":"1999","journal-title":"Virology"},{"key":"10.1016\/j.antiviral.2016.08.001_bib22","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1006\/viro.2002.1742","article-title":"Evaluation of a genetically modified reassortant H5N1 influenza A virus vaccine candidate generated by plasmid-based reverse genetics","volume":"305","author":"Subbarao","year":"2003","journal-title":"Virology"},{"key":"10.1016\/j.antiviral.2016.08.001_bib23","doi-asserted-by":"crossref","first-page":"8756","DOI":"10.1128\/JVI.00593-13","article-title":"N-linked glycosylation of the hemagglutinin protein influences virulence and antigenicity of the 1918 pandemic and seasonal H1N1 influenza A viruses","volume":"87","author":"Sun","year":"2013","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.antiviral.2016.08.001_bib24","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1111\/j.1600-065X.2008.00668.x","article-title":"Strategies and challenges for eliciting immunity against avian influenza virus in birds","volume":"225","author":"Swayne","year":"2008","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.antiviral.2016.08.001_bib25","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.cimid.2008.01.006","article-title":"Avian influenza vaccines and therapies for poultry","volume":"32","author":"Swayne","year":"2009","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"10.1016\/j.antiviral.2016.08.001_bib26","doi-asserted-by":"crossref","first-page":"132","DOI":"10.2307\/1592516","article-title":"Failure of a recombinant fowl poxvirus vaccine containing an avian influenza hemagglutinin gene to provide consistent protection against influenza in chickens preimmunized with a fowl pox vaccine","volume":"44","author":"Swayne","year":"2000","journal-title":"Avian Dis."},{"key":"10.1016\/j.antiviral.2016.08.001_bib27","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1637\/10183-041012-Review.1","article-title":"Impact of vaccines and vaccination on global control of avian influenza Avian influenza vaccines and therapies for poultry","volume":"56","author":"Swayne","year":"2012","journal-title":"Avian Dis."},{"key":"10.1016\/j.antiviral.2016.08.001_bib28","doi-asserted-by":"crossref","first-page":"12283","DOI":"10.1128\/JVI.01596-12","article-title":"Oligomeric recombinant H5 HA1 vaccine produced in bacteria protects ferrets from homologous and heterologous wild-type H5N1 influenza challenge and controls viral loads better than subunit H5N1 vaccine by eliciting high-affinity antibodies","volume":"86","author":"Verma","year":"2012","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.antiviral.2016.08.001_bib29","doi-asserted-by":"crossref","first-page":"6200","DOI":"10.1128\/JVI.00187-08","article-title":"Comparative efficacy of neutralizing antibodies elicited by recombinant hemagglutinin proteins from avian H5N1 influenza","volume":"82","author":"Wei","year":"2008","journal-title":"J.\u00a0Virol."},{"issue":"9","key":"10.1016\/j.antiviral.2016.08.001_bib30","doi-asserted-by":"crossref","first-page":"e12466","DOI":"10.1371\/journal.pone.0012466","article-title":"Enhanced immunogenicity of stabilized trimeric soluble influenza hemagglutinin","volume":"5","author":"Weldon","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.antiviral.2016.08.001_bib31","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1038\/289373a0","article-title":"Structural identification of the antibody-binding sites of Hong Kong influenza hemagglutinin and their involvement in antigenic variation","volume":"29","author":"Wiley","year":"1981","journal-title":"Nature"},{"key":"10.1016\/j.antiviral.2016.08.001_bib32","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1146\/annurev.iy.08.040190.003513","article-title":"Structural basic of immune recognition of influenza virus hemagglutinin","volume":"8","author":"Wilson","year":"1990","journal-title":"Ann. Rev. Immunol."}],"container-title":["Antiviral Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166354216302157?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166354216302157?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,9,24]],"date-time":"2020-09-24T19:59:28Z","timestamp":1600977568000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166354216302157"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9]]},"references-count":32,"alternative-id":["S0166354216302157"],"URL":"https:\/\/doi.org\/10.1016\/j.antiviral.2016.08.001","relation":{},"ISSN":["0166-3542"],"issn-type":[{"value":"0166-3542","type":"print"}],"subject":[],"published":{"date-parts":[[2016,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An avian influenza H5N1 virus vaccine candidate based on the extracellular domain produced in yeast system as subviral particles protects chickens from lethal challenge","name":"articletitle","label":"Article Title"},{"value":"Antiviral Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.antiviral.2016.08.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}