{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:24:00Z","timestamp":1790882640135,"version":"4.1.0"},"reference-count":28,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,4,27]],"date-time":"2024-04-27T00:00:00Z","timestamp":1714176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007046","name":"Wuhan University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007046","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFA0903200"],"award-info":[{"award-number":["2018YFA0903200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31920103001"],"award-info":[{"award-number":["31920103001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Acta Pharmaceutica Sinica B"],"published-print":{"date-parts":[[2024,9]]},"DOI":"10.1016\/j.apsb.2024.04.030","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T03:39:38Z","timestamp":1714707578000},"page":"4149-4163","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"title":["Glycodiversification of gentamicins through in\u00a0vivo glycosyltransferase swapping enabled the creation of novel hybrid aminoglycoside antibiotics with potent activity and low ototoxicity"],"prefix":"10.1016","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1276-4396","authenticated-orcid":false,"given":"Xinyun","family":"Jian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4394-6430","authenticated-orcid":false,"given":"Shijuan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengbing","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanzheng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter F.","family":"Leadlay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixin","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuling","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5720-9620","authenticated-orcid":false,"given":"Yuhui","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.apsb.2024.04.030_bib1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1021\/cb3005116","article-title":"Aminoglycoside antibiotics in the 21st century","volume":"8","author":"Becker","year":"2013","journal-title":"ACS Chem Biol"},{"key":"10.1016\/j.apsb.2024.04.030_bib2","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1038\/ja.2010.110","article-title":"Antibacterial to antifungal conversion of neamine aminoglycosides through alkyl modification. Strategy for reviving old drugs into agrofungicides","volume":"63","author":"Chang","year":"2010","journal-title":"J Antibiot"},{"key":"10.1016\/j.apsb.2024.04.030_bib3","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1038\/s41564-018-0138-2","article-title":"Topical application of aminoglycoside antibiotics enhances host resistance to viral infections in a microbiota-independent manner","volume":"3","author":"Gopinath","year":"2018","journal-title":"Nat Microbiol"},{"key":"10.1016\/j.apsb.2024.04.030_bib4","doi-asserted-by":"crossref","first-page":"3350","DOI":"10.1093\/nar\/gkq1277","article-title":"Rescue of non-sense mutated p53 tumor suppressor gene by aminoglycosides","volume":"39","author":"Floquet","year":"2011","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.apsb.2024.04.030_bib5","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1039\/C4MD00163J","article-title":"New trends in the use of aminoglycosides","volume":"5","author":"Fosso","year":"2014","journal-title":"MedChemComm"},{"key":"10.1016\/j.apsb.2024.04.030_bib6","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1039\/C7CS00407A","article-title":"Comprehensive review of chemical strategies for the preparation of new aminoglycosides and their biological activities","volume":"47","author":"Chandrika","year":"2018","journal-title":"Chem Soc Rev"},{"key":"10.1016\/j.apsb.2024.04.030_bib7","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.chembiol.2007.02.005","article-title":"Biosynthesis of butirosin: transfer and deprotection of the unique amino acid side chain","volume":"14","author":"Llewellyn","year":"2007","journal-title":"Chem Biol"},{"key":"10.1016\/j.apsb.2024.04.030_bib8","doi-asserted-by":"crossref","first-page":"3786","DOI":"10.1039\/b802248h","article-title":"Chemoenzymatic acylation of aminoglycoside antibiotics","volume":"28","author":"Llewellyn","year":"2008","journal-title":"Chem Commun (Camb)"},{"key":"10.1016\/j.apsb.2024.04.030_bib9","doi-asserted-by":"crossref","first-page":"2779","DOI":"10.1021\/acschembio.7b00466","article-title":"Structural basis of the selectivity of GenN, an aminoglycoside N-methyltransferase involved in gentamicin biosynthesis","volume":"12","author":"Bury","year":"2017","journal-title":"ACS Chem Biol"},{"key":"10.1016\/j.apsb.2024.04.030_bib10","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2021.725916","article-title":"Microbial enzymatic synthesis of amikacin analogs with antibacterial activity against multidrug-resistant pathogens","volume":"12","author":"Ban","year":"2021","journal-title":"Front Microbiol"},{"key":"10.1016\/j.apsb.2024.04.030_bib11","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1038\/nchembio.671","article-title":"Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation","volume":"7","author":"Park","year":"2011","journal-title":"Nat Chem Biol"},{"key":"10.1016\/j.apsb.2024.04.030_bib12","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1039\/b604709m","article-title":"Biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics","volume":"23","author":"Llewellyn","year":"2006","journal-title":"Nat Prod Rep"},{"key":"10.1016\/j.apsb.2024.04.030_bib13","doi-asserted-by":"crossref","first-page":"8399","DOI":"10.1073\/pnas.0803164105","article-title":"Genetic dissection of the biosynthetic route to gentamicin A2 by heterologous expression of its minimal gene set","volume":"105","author":"Park","year":"2008","journal-title":"Proc Natl Acad Sci U\u00a0S\u00a0A"},{"key":"10.1016\/j.apsb.2024.04.030_bib14","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.chembiol.2014.12.012","article-title":"Delineating the biosynthesis of gentamicin X2, the common precursor of the gentamicin C antibiotic complex","volume":"22","author":"Huang","year":"2015","journal-title":"Chem Biol"},{"key":"10.1016\/j.apsb.2024.04.030_bib15","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.1073\/pnas.1711603115","article-title":"Methyltransferases of gentamicin biosynthesis","volume":"115","author":"Li","year":"2018","journal-title":"Proc Natl Acad Sci U\u00a0S\u00a0A"},{"key":"10.1016\/j.apsb.2024.04.030_bib16","doi-asserted-by":"crossref","first-page":"8093","DOI":"10.1021\/ja312641f","article-title":"GenK-catalyzed C-6\u2032 methylation in the biosynthesis of gentamicin: isolation and characterization of a cobalamin-dependent radical SAM enzyme","volume":"135","author":"Kim","year":"2013","journal-title":"J Am Chem Soc"},{"key":"10.1016\/j.apsb.2024.04.030_bib17","doi-asserted-by":"crossref","first-page":"12274","DOI":"10.1021\/acscatal.1c03508","article-title":"Mechanistic insights into dideoxygenation in gentamicin biosynthesis","volume":"11","author":"Li","year":"2021","journal-title":"ACS Catal"},{"key":"10.1016\/j.apsb.2024.04.030_bib18","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1016\/j.chembiol.2014.03.005","article-title":"Specificity and promiscuity at the branch point in gentamicin biosynthesis","volume":"21","author":"Guo","year":"2014","journal-title":"Chem Biol"},{"key":"10.1016\/j.apsb.2024.04.030_bib19","doi-asserted-by":"crossref","first-page":"1933","DOI":"10.1002\/cbic.201500258","article-title":"Biosynthesis of epimers C2 and C2a in the gentamicin C complex","volume":"16","author":"Gu","year":"2015","journal-title":"ChemBioChem"},{"key":"10.1016\/j.apsb.2024.04.030_bib20","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1038\/s41589-018-0203-4","article-title":"Complete reconstitution of the diverse pathways of gentamicin B biosynthesis","volume":"15","author":"Ban","year":"2019","journal-title":"Nat Chem Biol"},{"key":"10.1016\/j.apsb.2024.04.030_bib21","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1007\/s00253-008-1793-7","article-title":"Analysis of functions in plasmid pHZ1358 influencing its genetic and structural stability in Streptomyces lividans 1326","volume":"82","author":"Sun","year":"2009","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.apsb.2024.04.030_bib22","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1008953","article-title":"THOC1 deficiency leads to late-onset nonsyndromic hearing loss through p53-mediated hair cell apoptosis","volume":"16","author":"Zhang","year":"2020","journal-title":"PLoS Genet"},{"key":"10.1016\/j.apsb.2024.04.030_bib23","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0055359","article-title":"Dimethyl sulfoxide (DMSO) exacerbates cisplatin-induced sensory hair cell death in zebrafish (Danio rerio)","volume":"8","author":"Uribe","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.apsb.2024.04.030_bib24","doi-asserted-by":"crossref","DOI":"10.3389\/fncel.2015.00131","article-title":"Sensory hair cell death and regeneration in fishes","volume":"9","author":"Monroe","year":"2015","journal-title":"Front Cell Neurosci"},{"key":"10.1016\/j.apsb.2024.04.030_bib25","doi-asserted-by":"crossref","first-page":"F923","DOI":"10.1152\/ajprenal.00386.2004","article-title":"Acute renal failure in zebrafish: a novel system to study a complex disease","volume":"288","author":"Hentschel","year":"2005","journal-title":"Am J Physiol Renal Physiol"},{"key":"10.1016\/j.apsb.2024.04.030_bib26","series-title":"The zebrafish book: a guide for the laboratory use of zebrafish Danio (Brachydanio) rerio","author":"Westerfield","year":"2000"},{"key":"10.1016\/j.apsb.2024.04.030_bib27","doi-asserted-by":"crossref","first-page":"3231","DOI":"10.1523\/JNEUROSCI.2216-16.2017","article-title":"Functional analysis of the transmembrane and cytoplasmic domains of pcdh15a in zebrafish hair cells","volume":"37","author":"Maeda","year":"2017","journal-title":"J Neurosci"},{"key":"10.1016\/j.apsb.2024.04.030_bib28","doi-asserted-by":"crossref","first-page":"32423","DOI":"10.1073\/pnas.2013065117","article-title":"Dissociating antibacterial from ototoxic effects of gentamicin C-subtypes","volume":"117","author":"O'Sullivan","year":"2020","journal-title":"Proc Natl Acad Sci U\u00a0S\u00a0A"}],"container-title":["Acta Pharmaceutica Sinica B"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211383524001710?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211383524001710?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T20:55:46Z","timestamp":1725656146000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2211383524001710"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9]]},"references-count":28,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["S2211383524001710"],"URL":"https:\/\/doi.org\/10.1016\/j.apsb.2024.04.030","relation":{},"ISSN":["2211-3835"],"issn-type":[{"value":"2211-3835","type":"print"}],"subject":[],"published":{"date-parts":[[2024,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Glycodiversification of gentamicins through in\u00a0vivo glycosyltransferase swapping enabled the creation of novel hybrid aminoglycoside antibiotics with potent activity and low ototoxicity","name":"articletitle","label":"Article Title"},{"value":"Acta Pharmaceutica Sinica B","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.apsb.2024.04.030","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier B.V. on behalf of Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences.","name":"copyright","label":"Copyright"}]}}