{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T11:10:35Z","timestamp":1793013035632,"version":"4.5.0"},"reference-count":118,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T00:00:00Z","timestamp":1750032000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100015226","name":"Shengjing Hospital","doi-asserted-by":"publisher","award":["202003"],"award-info":[{"award-number":["202003"]}],"id":[{"id":"10.13039\/501100015226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82371647"],"award-info":[{"award-number":["82371647"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013290","name":"National Key Research and Development Program of China Stem Cell and Translational Research","doi-asserted-by":"publisher","award":["2024YFC2706900"],"award-info":[{"award-number":["2024YFC2706900"]}],"id":[{"id":"10.13039\/501100013290","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018559","name":"Shenyang Young and Middle-aged Science and Technology Innovation Talent Support Program","doi-asserted-by":"publisher","award":["RC210436"],"award-info":[{"award-number":["RC210436"]}],"id":[{"id":"10.13039\/501100018559","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Ageing Research Reviews"],"published-print":{"date-parts":[[2025,8]]},"DOI":"10.1016\/j.arr.2025.102807","type":"journal-article","created":{"date-parts":[[2025,6,15]],"date-time":"2025-06-15T10:11:16Z","timestamp":1749982276000},"page":"102807","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Ovarian aging at single-cell resolution: Current paradigms and perspectives"],"prefix":"10.1016","volume":"110","author":[{"given":"Junzhi","family":"Liang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shurui","family":"Gai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinni","family":"Na","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongyu","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duo","family":"Zi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijing","family":"Na","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyan","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangfang","family":"Bi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4895-1185","authenticated-orcid":false,"given":"Da","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.arr.2025.102807_bib1","first-page":"25","article-title":"scATAC-seq preprocessing and imputation evaluation system for visualization, clustering and digital footprinting","author":"Akhtyamov","year":"2023","journal-title":"Brief. Bioinform"},{"key":"10.1016\/j.arr.2025.102807_bib2","doi-asserted-by":"crossref","DOI":"10.1111\/acel.13259","article-title":"Ovarian stiffness increases with age in the mammalian ovary and depends on collagen and hyaluronan matrices","volume":"19","author":"Amargant","year":"2020","journal-title":"Aging Cell"},{"key":"10.1016\/j.arr.2025.102807_bib3","doi-asserted-by":"crossref","DOI":"10.1007\/s11357-024-01322-w","article-title":"Systemic low-dose anti-fibrotic treatment attenuates ovarian aging in the mouse","author":"Amargant","year":"2024","journal-title":"Geroscience"},{"key":"10.1016\/j.arr.2025.102807_bib4","doi-asserted-by":"crossref","first-page":"1711","DOI":"10.1038\/s43587-024-00769-y","article-title":"Hallmarks of female reproductive aging in physiologic aging mice","volume":"4","author":"Balough","year":"2024","journal-title":"Nat. Aging"},{"key":"10.1016\/j.arr.2025.102807_bib5","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1038\/s41580-023-00615-w","article-title":"The technological landscape and applications of single-cell multi-omics","volume":"24","author":"Baysoy","year":"2023","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.arr.2025.102807_bib6","first-page":"12","article-title":"Single-cell analysis of the aged ovarian immune system reveals a shift towards adaptive immunity and attenuated cell function","author":"Ben Yaakov","year":"2023","journal-title":"Elife"},{"key":"10.1016\/j.arr.2025.102807_bib7","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1093\/humrep\/deae254","article-title":"Immune system regulation of physiological and pathological aspects of the ovarian follicle pool throughout the female reproductive lifespan","volume":"40","author":"Cacciottola","year":"2025","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.arr.2025.102807_bib8","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1089\/dna.2022.0449","article-title":"Circular RNA circFoxo3 promotes granulosa cell apoptosis under oxidative stress through regulation of FOXO3 protein","volume":"41","author":"Chen","year":"2022","journal-title":"DNA Cell Biol."},{"key":"10.1016\/j.arr.2025.102807_bib9","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abq3745","article-title":"DeepVelo: single-cell transcriptomic deep velocity field learning with neural ordinary differential equations","volume":"8","author":"Chen","year":"2022","journal-title":"Sci. Adv."},{"key":"10.1016\/j.arr.2025.102807_bib10","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1038\/s41592-024-02201-0","article-title":"scGPT: toward building a foundation model for single-cell multi-omics using generative AI","volume":"21","author":"Cui","year":"2024","journal-title":"Nat. Methods"},{"key":"10.1016\/j.arr.2025.102807_bib11","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1186\/s13048-024-01492-3","article-title":"Stem cell-based therapeutic potential in female ovarian aging and infertility","volume":"17","author":"Cui","year":"2024","journal-title":"J. Ovarian Res"},{"key":"10.1016\/j.arr.2025.102807_bib12","doi-asserted-by":"crossref","DOI":"10.3390\/ijms241612560","article-title":"miR-143-3p promotes ovarian granulosa cell senescence and inhibits estradiol synthesis by targeting UBE2E3 and LHCGR","volume":"24","author":"Deng","year":"2023","journal-title":"Int J. Mol. Sci."},{"key":"10.1016\/j.arr.2025.102807_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.arr.2021.101378","article-title":"Mitochondria: their relevance during oocyte ageing","volume":"70","author":"van der Reest","year":"2021","journal-title":"Ageing Res Rev."},{"key":"10.1016\/j.arr.2025.102807_bib14","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1111\/acel.12617","article-title":"Short-term rapamycin treatment increases ovarian lifespan in young and middle-aged female mice","volume":"16","author":"Dou","year":"2017","journal-title":"Aging Cell"},{"key":"10.1016\/j.arr.2025.102807_bib15","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1210\/er.2018-00075","article-title":"Ovulation: parallels with inflammatory processes","volume":"40","author":"Duffy","year":"2019","journal-title":"Endocr. Rev."},{"key":"10.1016\/j.arr.2025.102807_bib16","doi-asserted-by":"crossref","first-page":"3164","DOI":"10.1038\/s41467-019-11036-9","article-title":"Single-cell reconstruction of follicular remodeling in the human adult ovary","volume":"10","author":"Fan","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.arr.2025.102807_bib17","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1007\/s10815-024-03117-6","article-title":"Evaluation of telomere length and telomerase activity on predicting in vitro fertilization treatment outcomes","volume":"41","author":"Fragkiadaki","year":"2024","journal-title":"J. Assist Reprod. Genet"},{"key":"10.1016\/j.arr.2025.102807_bib18","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1093\/biolre\/ioaa100","article-title":"IRX3 and IRX5 collaborate during ovary development and follicle formation to establish responsive granulosa cells in the adult mouse","volume":"103","author":"Fu","year":"2020","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.arr.2025.102807_bib19","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1007\/s11357-019-00087-x","article-title":"Effect of caloric restriction and rapamycin on ovarian aging in mice","volume":"41","author":"Garcia","year":"2019","journal-title":"Geroscience"},{"key":"10.1016\/j.arr.2025.102807_bib20","article-title":"Short-term moderate caloric restriction in the rhesus macaque attenuates markers of ovarian aging in select populations","volume":"17","author":"Gargus","year":"2025","journal-title":"Aging (Albany NY)"},{"key":"10.1016\/j.arr.2025.102807_bib21","doi-asserted-by":"crossref","DOI":"10.3390\/biom13010047","article-title":"Application of single-Cell RNA sequencing in ovarian development","volume":"13","author":"Gong","year":"2022","journal-title":"Biomolecules"},{"key":"10.1016\/j.arr.2025.102807_bib22","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1186\/s13048-024-01448-7","article-title":"Ovarian fibrosis: molecular mechanisms and potential therapeutic targets","volume":"17","author":"Gu","year":"2024","journal-title":"J. Ovarian Res"},{"key":"10.1016\/j.arr.2025.102807_bib23","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/s41580-024-00768-2","article-title":"Profiling cell identity and tissue architecture with single-cell and spatial transcriptomics","volume":"26","author":"Gulati","year":"2025","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.arr.2025.102807_bib24","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1093\/humupd\/dmae020","article-title":"Ovarian microenvironment: challenges and opportunities in protecting against chemotherapy-associated ovarian damage","volume":"30","author":"Guo","year":"2024","journal-title":"Hum. Reprod. Update"},{"key":"10.1016\/j.arr.2025.102807_bib25","doi-asserted-by":"crossref","first-page":"11221","DOI":"10.2147\/JIR.S490996","article-title":"Single-Cell RNA Sequencing Reveals That C5AR1 in Follicle Monocyte Cells Could Predict the Development of POI","volume":"17","author":"Han","year":"2024","journal-title":"J. Inflamm. Res"},{"key":"10.1016\/j.arr.2025.102807_bib26","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1093\/biolre\/ioae157","article-title":"Single-cell RNA sequencing reveals common interactions between follicle immune cells and granulosa cells in premature ovarian insufficiency patients","volume":"112","author":"Han","year":"2025","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.arr.2025.102807_bib27","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1038\/s41592-024-02305-7","article-title":"Large-scale foundation model on single-cell transcriptomics","volume":"21","author":"Hao","year":"2024","journal-title":"Nat. Methods"},{"key":"10.1016\/j.arr.2025.102807_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2025.141455","article-title":"TGF-\u03b2 signaling in the ovary: emerging roles in development and disease","volume":"306","author":"He","year":"2025","journal-title":"Int J. Biol. Macromol."},{"key":"10.1016\/j.arr.2025.102807_bib29","doi-asserted-by":"crossref","DOI":"10.1111\/acel.13304","article-title":"Mtor inhibition by INK128 extends functions of the ovary reconstituted from germline stem cells in aging and premature aging mice","volume":"20","author":"Heng","year":"2021","journal-title":"Aging Cell"},{"key":"10.1016\/j.arr.2025.102807_bib30","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/s41514-024-00157-1","article-title":"The role of cellular senescence in ovarian aging","volume":"10","author":"Hense","year":"2024","journal-title":"NPJ Aging"},{"key":"10.1016\/j.arr.2025.102807_bib31","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.3389\/fimmu.2019.01187","article-title":"The Multi-faceted Ecto-enzyme CD38: Roles in Immunomodulation, Cancer, Aging, and Metabolic Diseases","volume":"10","author":"Hogan","year":"2019","journal-title":"Front Immunol."},{"key":"10.1016\/j.arr.2025.102807_bib32","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.freeradbiomed.2020.12.434","article-title":"Adverse effect of superoxide-induced mitochondrial damage in granulosa cells on follicular development in mouse ovaries","volume":"163","author":"Hoque","year":"2021","journal-title":"Free Radic. Biol. Med"},{"key":"10.1016\/j.arr.2025.102807_bib33","doi-asserted-by":"crossref","first-page":"1692","DOI":"10.1038\/s42003-024-07305-z","article-title":"Age-associated aberrations of the cumulus-oocyte interaction and in the zona pellucida structure reduce fertility in female mice","volume":"7","author":"Ishikawa-Yamauchi","year":"2024","journal-title":"Commun. Biol."},{"key":"10.1016\/j.arr.2025.102807_bib34","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1038\/s43587-023-00552-5","article-title":"A single-cell atlas of the aging mouse ovary","volume":"4","author":"Isola","year":"2024","journal-title":"Nat. Aging"},{"key":"10.1016\/j.arr.2025.102807_bib35","doi-asserted-by":"crossref","DOI":"10.1007\/s11357-024-01465-w","article-title":"Canagliflozin treatment prevents follicular exhaustion and attenuates hallmarks of ovarian aging in genetically heterogenous mice","author":"Isola","year":"2024","journal-title":"Geroscience"},{"key":"10.1016\/j.arr.2025.102807_bib36","doi-asserted-by":"crossref","first-page":"2818","DOI":"10.1007\/s11427-022-2335-6","article-title":"Analyzing the cellular and molecular atlas of ovarian mesenchymal cells provides a strategy against female reproductive aging","volume":"66","author":"Jia","year":"2023","journal-title":"Sci. China Life Sci."},{"key":"10.1016\/j.arr.2025.102807_bib37","doi-asserted-by":"crossref","DOI":"10.1093\/lifemedi\/lnae041","article-title":"Single-cell RNA sequencing reveals the intercellular crosstalk and the regulatory landscape of stromal cells during the whole life of the mouse ovary","volume":"3","author":"Jiang","year":"2024","journal-title":"Life Med"},{"key":"10.1016\/j.arr.2025.102807_bib38","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s11427-023-2360-3","article-title":"Cellular atlases of ovarian microenvironment alterations by diet and genetically-induced obesity","volume":"67","author":"Jiang","year":"2024","journal-title":"Sci. China Life Sci."},{"key":"10.1016\/j.arr.2025.102807_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2023.113455","article-title":"Simultaneous deep transcriptome and proteome profiling in a single mouse oocyte","volume":"42","author":"Jiang","year":"2023","journal-title":"Cell Rep."},{"key":"10.1016\/j.arr.2025.102807_bib40","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1038\/s43587-024-00762-5","article-title":"Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses","volume":"5","author":"Jin","year":"2025","journal-title":"Nat. Aging"},{"key":"10.1016\/j.arr.2025.102807_bib41","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adm7506","article-title":"Cellular atlas of the human ovary using morphologically guided spatial transcriptomics and single-cell sequencing","volume":"10","author":"Jones","year":"2024","journal-title":"Sci. Adv."},{"key":"10.1016\/j.arr.2025.102807_bib42","doi-asserted-by":"crossref","DOI":"10.1002\/ctm2.70043","article-title":"Single-nucleus and spatial transcriptomics of paediatric ovary: Molecular insights into the dysregulated signalling pathways underlying premature ovarian insufficiency in classic galactosemia","volume":"14","author":"Kavarthapu","year":"2024","journal-title":"Clin. Transl. Med"},{"key":"10.1016\/j.arr.2025.102807_bib43","article-title":"Demographic and evolutionary trends in ovarian function and aging","author":"Laisk","year":"2019","journal-title":"Hum. Reprod. Update 25 3450"},{"key":"10.1016\/j.arr.2025.102807_bib44","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abq1475","article-title":"Metformin prevents age-associated ovarian fibrosis by modulating the immune landscape in female mice","volume":"8","author":"Landry","year":"2022","journal-title":"Sci. Adv."},{"key":"10.1016\/j.arr.2025.102807_bib45","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1186\/s13287-023-03616-w","article-title":"Exploring distinct properties of endometrial stem cells through advanced single-cell analysis platforms","volume":"14","author":"Lee","year":"2023","journal-title":"Stem Cell Res Ther."},{"key":"10.1016\/j.arr.2025.102807_bib46","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2022.111838","article-title":"A molecular atlas of the human postmenopausal fallopian tube and ovary from single-cell RNA and ATAC sequencing","volume":"41","author":"Lengyel","year":"2022","journal-title":"Cell Rep."},{"key":"10.1016\/j.arr.2025.102807_bib47","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.freeradbiomed.2022.12.084","article-title":"Intermittent fasting reverses the declining quality of aged oocytes","volume":"195","author":"Li","year":"2023","journal-title":"Free Radic. Biol. Med"},{"key":"10.1016\/j.arr.2025.102807_bib48","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2021.730196","article-title":"The Signaling Pathways Involved in Ovarian Follicle Development","volume":"12","author":"Li","year":"2021","journal-title":"Front Physiol."},{"key":"10.1016\/j.arr.2025.102807_bib49","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2213026119","article-title":"Perivascular cells support folliculogenesis in the developing ovary","volume":"119","author":"Li","year":"2022","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.arr.2025.102807_bib50","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1186\/s13045-024-01596-9","article-title":"Spatial multi-omics: deciphering technological landscape of integration of multi-omics and its applications","volume":"17","author":"Liu","year":"2024","journal-title":"J. Hematol. Oncol."},{"key":"10.1016\/j.arr.2025.102807_bib51","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1093\/procel\/pwad063","article-title":"Aging hallmarks of the primate ovary revealed by spatiotemporal transcriptomics","volume":"15","author":"Lu","year":"2024","journal-title":"Protein Cell"},{"key":"10.1016\/j.arr.2025.102807_bib52","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1002\/mrd.23648","article-title":"Single-cell RNA-sequencing of retrieved human oocytes and eggs in clinical practice and for human ovarian cell atlasing","volume":"89","author":"Machlin","year":"2022","journal-title":"Mol. Reprod. Dev."},{"key":"10.1016\/j.arr.2025.102807_bib53","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1158\/1078-0432.CCR-19-0603","article-title":"Metformin Abrogates Age-Associated Ovarian Fibrosis","volume":"26","author":"McCloskey","year":"2020","journal-title":"Clin. Cancer Res"},{"key":"10.1016\/j.arr.2025.102807_bib54","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-96263-1","article-title":"Dynamic landscape of chromatin accessibility and transcriptomic changes during differentiation of human embryonic stem cells into dopaminergic neurons","volume":"11","author":"Mel\u00e9ndez-Ram\u00edrez","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.arr.2025.102807_bib55","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2020.107987","article-title":"Nicotinamide Mononucleotide Supplementation Reverses the Declining Quality of Maternally Aged Oocytes","volume":"32","author":"Miao","year":"2020","journal-title":"Cell Rep."},{"key":"10.1016\/j.arr.2025.102807_bib56","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1038\/s41467-024-55509-y","article-title":"Physiological premature aging of ovarian blood vessels leads to decline in fertility in middle-aged mice","volume":"16","author":"Mu","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.arr.2025.102807_bib57","doi-asserted-by":"crossref","unstructured":"Multiomics atlas reveals molecular and genetic drivers of human ovarian aging. Nat Aging 5, 186-187. 2025.","DOI":"10.1038\/s43587-025-00821-5"},{"key":"10.1016\/j.arr.2025.102807_bib58","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abc7409","article-title":"Inhibition of the NLRP3 inflammasome prevents ovarian aging","volume":"7","author":"Navarro-Pando","year":"2021","journal-title":"Sci. Adv."},{"key":"10.1016\/j.arr.2025.102807_bib59","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.1093\/humrep\/deaa071","article-title":"A distinctive epigenetic ageing profile in human granulosa cells","volume":"35","author":"Olsen","year":"2020","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.arr.2025.102807_bib60","doi-asserted-by":"crossref","DOI":"10.3390\/genes12081186","article-title":"Hyaluronan and collagen are prominent extracellular matrix components in bovine and porcine ovaries","volume":"12","author":"Parkes","year":"2021","journal-title":"Genes (Basel)"},{"key":"10.1016\/j.arr.2025.102807_bib61","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1186\/s13287-024-03698-0","article-title":"Efficacy and safety of mesenchymal stem cell therapy for ovarian ageing in a mouse model","volume":"15","author":"Pei","year":"2024","journal-title":"Stem Cell Res Ther."},{"key":"10.1016\/j.arr.2025.102807_bib62","doi-asserted-by":"crossref","first-page":"2484","DOI":"10.1038\/s41467-023-38121-4","article-title":"Reconstruction of the cell pseudo-space from single-cell RNA sequencing data with scSpace","volume":"14","author":"Qian","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.arr.2025.102807_bib63","doi-asserted-by":"crossref","DOI":"10.1016\/j.ajog.2025.02.006","article-title":"Nicotinamide mononucleotide supplementation improves oocyte developmental competence in different ovarian damage conditions","author":"Ram\u00edrez-Mart\u00edn","year":"2025","journal-title":"Am. J. Obstet. Gynecol."},{"key":"10.1016\/j.arr.2025.102807_bib64","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/humupd\/dmaa043","article-title":"Metabolic co-dependence of the oocyte and cumulus cells: essential role in determining oocyte developmental competence","volume":"27","author":"Richani","year":"2021","journal-title":"Hum. Reprod. Update"},{"key":"10.1016\/j.arr.2025.102807_bib65","doi-asserted-by":"crossref","DOI":"10.1016\/j.ajog.2024.05.046","article-title":"The adult ovary at single cell resolution: an expert review","volume":"232","author":"Rooda","year":"2025","journal-title":"Am. J. Obstet. Gynecol."},{"key":"10.1016\/j.arr.2025.102807_bib66","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2022.104819","article-title":"Spatially resolved transcriptomic profiling of ovarian aging in mice","volume":"25","author":"Russ","year":"2022","journal-title":"iScience"},{"key":"10.1016\/j.arr.2025.102807_bib67","doi-asserted-by":"crossref","DOI":"10.1210\/clinem\/dgaa764","article-title":"The menopause transition: signs, symptoms, and management options","volume":"106","author":"Santoro","year":"2021","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"10.1016\/j.arr.2025.102807_bib68","doi-asserted-by":"crossref","DOI":"10.1016\/j.advnut.2024.100273","article-title":"Antioxidants and fertility in women with ovarian aging: a systematic review and meta-analysis","volume":"15","author":"Shang","year":"2024","journal-title":"Adv. Nutr."},{"key":"10.1016\/j.arr.2025.102807_bib69","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1186\/s13048-023-01300-4","article-title":"The stromal microenvironment and ovarian aging: mechanisms and therapeutic opportunities","volume":"16","author":"Shen","year":"2023","journal-title":"J. Ovarian Res"},{"key":"10.1016\/j.arr.2025.102807_bib70","doi-asserted-by":"crossref","DOI":"10.1111\/cpr.13516","article-title":"A spatiotemporal gene expression and cell atlases of the developing rat ovary","volume":"56","author":"Shi","year":"2023","journal-title":"Cell Prolif."},{"key":"10.1016\/j.arr.2025.102807_bib71","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.gpb.2020.06.010","article-title":"Profiling chromatin accessibility at single-cell resolution","volume":"19","author":"Sinha","year":"2021","journal-title":"Genom. Proteom. Bioinforma."},{"key":"10.1016\/j.arr.2025.102807_bib72","doi-asserted-by":"crossref","first-page":"1384","DOI":"10.1038\/s43587-024-00670-8","article-title":"An NAD+-dependent metabolic checkpoint regulates hematopoietic stem cell activation and aging","volume":"4","author":"Song","year":"2024","journal-title":"Nat. Aging"},{"key":"10.1016\/j.arr.2025.102807_bib73","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1038\/nmeth.1315","article-title":"mRNA-Seq whole-transcriptome analysis of a single cell","volume":"6","author":"Tang","year":"2009","journal-title":"Nat. Methods"},{"key":"10.1016\/j.arr.2025.102807_bib74","doi-asserted-by":"crossref","DOI":"10.3389\/fendo.2023.1282658","article-title":"New insight into the role of macrophages in ovarian function and ovarian aging","volume":"14","author":"Tang","year":"2023","journal-title":"Front Endocrinol. (Lausanne)"},{"key":"10.1016\/j.arr.2025.102807_bib75","doi-asserted-by":"crossref","first-page":"2623","DOI":"10.1152\/physrev.00032.2022","article-title":"Making a good egg: human oocyte health, aging, and in vitro development","volume":"103","author":"Telfer","year":"2023","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.arr.2025.102807_bib76","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1038\/s41586-023-06139-9","article-title":"Transfer learning enables predictions in network biology","volume":"618","author":"Theodoris","year":"2023","journal-title":"Nature"},{"key":"10.1016\/j.arr.2025.102807_bib77","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abn4564","article-title":"Female reproductive life span is extended by targeted removal of fibrotic collagen from the mouse ovary","volume":"8","author":"Umehara","year":"2022","journal-title":"Sci. Adv."},{"key":"10.1016\/j.arr.2025.102807_bib78","article-title":"Comparative evaluation of ACetic - MEthanol high salt dissociation approach for single-cell transcriptomics of frozen human tissues","volume":"12","author":"Utkina","year":"2024","journal-title":"Front Cell Dev. Biol."},{"key":"10.1016\/j.arr.2025.102807_bib79","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1038\/s41576-023-00580-2","article-title":"Methods and applications for single-cell and spatial multi-omics","volume":"24","author":"Vandereyken","year":"2023","journal-title":"Nat. Rev. Genet"},{"key":"10.1016\/j.arr.2025.102807_bib80","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1038\/s41467-020-14936-3","article-title":"Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells","volume":"11","author":"Wagner","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.arr.2025.102807_bib81","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1038\/s43587-024-00697-x","article-title":"Rejuvenation of aged oocyte through exposure to young follicular microenvironment","volume":"4","author":"Wang","year":"2024","journal-title":"Nat. Aging"},{"key":"10.1016\/j.arr.2025.102807_bib82","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.3001025","article-title":"Single-cell transcriptome landscape of ovarian cells during primordial follicle assembly in mice","volume":"18","author":"Wang","year":"2020","journal-title":"PLoS Biol."},{"key":"10.1016\/j.arr.2025.102807_bib83","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/s13287-023-03631-x","article-title":"Cellular and molecular mechanisms of highly active mesenchymal stem cells in the treatment of senescence of rhesus monkey ovary","volume":"15","author":"Wang","year":"2024","journal-title":"Stem Cell Res Ther."},{"key":"10.1016\/j.arr.2025.102807_bib84","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.jare.2021.09.001","article-title":"A comparative study of Mesenchymal Stem Cells transplantation approach to antagonize age-associated ovarian hypofunction with consideration of safety and efficiency","volume":"38","author":"Wang","year":"2022","journal-title":"J. Adv. Res"},{"key":"10.1016\/j.arr.2025.102807_bib85","doi-asserted-by":"crossref","DOI":"10.1016\/j.cell.2020.01.009","article-title":"Single-cell transcriptomic atlas of primate ovarian aging","volume":"180","author":"Wang","year":"2020","journal-title":"Cell"},{"key":"10.1016\/j.arr.2025.102807_bib86","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1186\/s13287-024-03997-6","article-title":"Transplantation of human umbilical cord-derived mesenchymal stem cells improves age-related ovarian functional decline via regulating the local renin-angiotensin system on inflammation and oxidative stress","volume":"15","author":"Wei","year":"2024","journal-title":"Stem Cell Res Ther."},{"key":"10.1016\/j.arr.2025.102807_bib87","doi-asserted-by":"crossref","DOI":"10.1016\/j.arr.2024.102245","article-title":"Multiomics insights into the female reproductive aging","volume":"95","author":"Wei","year":"2024","journal-title":"Ageing Res Rev."},{"key":"10.1016\/j.arr.2025.102807_bib88","doi-asserted-by":"crossref","DOI":"10.1016\/j.omtn.2022.11.020","article-title":"Single-cell profiling of mouse and primate ovaries identifies high levels of EGFR for stromal cells in ovarian aging","volume":"31","author":"Wei","year":"2023","journal-title":"Mol. Ther. Nucleic Acids"},{"issue":"2020","key":"10.1016\/j.arr.2025.102807_bib89","article-title":"Single-cell transcriptome analysis of the mouse and primate ovaries reveals oocyte-specific expression patterns of risk genes in ovarian aging","volume":"4","author":"Wei","year":"2023","journal-title":"MedComm"},{"key":"10.1016\/j.arr.2025.102807_bib90","first-page":"187","article-title":"The cycling and aging mouse female reproductive tract at single-cell resolution","author":"Winkler","year":"2024","journal-title":"Cell"},{"key":"10.1016\/j.arr.2025.102807_bib91","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1016\/j.tem.2025.01.005","article-title":"Hallmarks of ovarian aging","volume":"36","author":"Wu","year":"2025","journal-title":"Trends Endocrinol. Metab."},{"key":"10.1016\/j.arr.2025.102807_bib92","doi-asserted-by":"crossref","DOI":"10.3390\/biom12020231","article-title":"Single-cell sequencing reveals an intrinsic heterogeneity of the preovulatory follicular microenvironment","volume":"12","author":"Wu","year":"2022","journal-title":"Biomolecules"},{"key":"10.1016\/j.arr.2025.102807_bib93","doi-asserted-by":"crossref","DOI":"10.1016\/j.arr.2022.101683","article-title":"Adipose tissue and ovarian aging: Potential mechanism and protective strategies","volume":"80","author":"Wu","year":"2022","journal-title":"Ageing Res Rev."},{"key":"10.1016\/j.arr.2025.102807_bib94","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1038\/s43587-024-00607-1","article-title":"Spatiotemporal transcriptomic changes of human ovarian aging and the regulatory role of FOXP1","volume":"4","author":"Wu","year":"2024","journal-title":"Nat. Aging"},{"key":"10.1016\/j.arr.2025.102807_bib95","doi-asserted-by":"crossref","DOI":"10.1016\/j.redox.2025.103584","article-title":"A high-resolution N-glycoproteome landscape of aging mouse ovary","volume":"81","author":"Wu","year":"2025","journal-title":"Redox Biol."},{"key":"10.1016\/j.arr.2025.102807_bib96","doi-asserted-by":"crossref","DOI":"10.1016\/j.devcel.2024.09.009","article-title":"RNA surveillance by the RNA helicase MTR4 determines volume of mouse oocytes","volume":"60","author":"Wu","year":"2025","journal-title":"Dev. Cell"},{"key":"10.1016\/j.arr.2025.102807_bib97","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.3727\/096368914X685267","article-title":"Mesenchymal Stem Cells Enhance Angiogenesis and Follicle Survival in Human Cryopreserved Ovarian Cortex Transplantation","volume":"24","author":"Xia","year":"2015","journal-title":"Cell Transpl."},{"key":"10.1016\/j.arr.2025.102807_bib98","doi-asserted-by":"crossref","DOI":"10.1002\/ctm2.1071","article-title":"Macrophage-derived extracellular vesicles regulate follicular activation and improve ovarian function in old mice by modulating local environment","volume":"12","author":"Xiao","year":"2022","journal-title":"Clin. Transl. Med"},{"key":"10.1016\/j.arr.2025.102807_bib99","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1186\/s40104-023-00948-8","article-title":"Single-cell transcriptomic atlas of goat ovarian aging","volume":"14","author":"Xu","year":"2023","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"10.1016\/j.arr.2025.102807_bib100","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkaf138","article-title":"Deep learning-based cell-specific gene regulatory networks inferred from single-cell multiome data","volume":"53","author":"Xu","year":"2025","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.arr.2025.102807_bib101","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1186\/s12943-025-02240-x","article-title":"From morphology to single-cell molecules: high-resolution 3D histology in biomedicine","volume":"24","author":"Xu","year":"2025","journal-title":"Mol. Cancer"},{"key":"10.1016\/j.arr.2025.102807_bib102","article-title":"Integrated analyses of the single-cell ATAC-seq and RNA-seq reveal the epigenetic landscape of human ovarian aging","volume":"2021","author":"Xu","year":"2021","journal-title":"bioRxiv"},{"key":"10.1016\/j.arr.2025.102807_bib103","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.molmed.2024.05.007","article-title":"Metabolic disorder: the dark side of ovarian aging","volume":"30","author":"Xue","year":"2024","journal-title":"Trends Mol. Med"},{"key":"10.1016\/j.arr.2025.102807_bib104","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1038\/s41421-024-00726-4","article-title":"Stem cell transplantation extends the reproductive life span of naturally aging cynomolgus monkeys","volume":"10","author":"Yan","year":"2024","journal-title":"Cell Discov."},{"key":"10.1016\/j.arr.2025.102807_bib105","doi-asserted-by":"crossref","DOI":"10.1016\/j.stem.2021.04.012","article-title":"Decoding dynamic epigenetic landscapes in human oocytes using single-cell multi-omics sequencing","volume":"28","author":"Yan","year":"2021","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.arr.2025.102807_bib106","doi-asserted-by":"crossref","DOI":"10.1016\/j.freeradbiomed.2020.05.003","article-title":"Increasing ovarian NAD+ levels improve mitochondrial functions and reverse ovarian aging","volume":"156","author":"Yang","year":"2020","journal-title":"Free Radic. Biol. Med"},{"key":"10.1016\/j.arr.2025.102807_bib107","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1038\/s43587-023-00532-9","article-title":"NADase CD38 is a key determinant of ovarian aging","volume":"4","author":"Yang","year":"2024","journal-title":"Nat. Aging"},{"key":"10.1016\/j.arr.2025.102807_bib108","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1038\/s41592-024-02558-2","article-title":"Enhanced sensitivity and scalability with a Chip-Tip workflow enables deep single-cell proteomics","volume":"22","author":"Ye","year":"2025","journal-title":"Nat. Methods"},{"key":"10.1016\/j.arr.2025.102807_bib109","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1186\/s13048-019-0528-y","article-title":"Can ovaries be preserved after an ovarian arteriovenous disconnection? One case report and a review of surgical treatment using Da Vinci robots for aggressive ovarian fibromatosis","volume":"12","author":"Ying","year":"2019","journal-title":"J. Ovarian Res"},{"key":"10.1016\/j.arr.2025.102807_bib110","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1093\/humrep\/deae132","article-title":"Unveiling the role of chronic inflammation in ovarian aging: insights into mechanisms and clinical implications","volume":"39","author":"Zeng","year":"2024","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.arr.2025.102807_bib111","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1093\/humupd\/dmaf002","article-title":"Harnessing omics data for drug discovery and development in ovarian aging","volume":"31","author":"Zhang","year":"2025","journal-title":"Hum. Reprod. Update"},{"key":"10.1016\/j.arr.2025.102807_bib112","doi-asserted-by":"crossref","DOI":"10.1016\/j.redox.2021.102215","article-title":"Melatonin improves the quality of maternally aged oocytes by maintaining intercellular communication and antioxidant metabolite supply","volume":"49","author":"Zhang","year":"2022","journal-title":"Redox Biol."},{"key":"10.1016\/j.arr.2025.102807_bib113","doi-asserted-by":"crossref","DOI":"10.1007\/s11357-025-01556-2","article-title":"A multi-omic single-cell landscape of the aging mouse ovary","author":"Zhang","year":"2025","journal-title":"Geroscience"},{"key":"10.1016\/j.arr.2025.102807_bib114","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1093\/biolre\/ioac217","article-title":"Quantitative proteomic analysis uncovers protein-expression profiles during gonadotropin-dependent folliculogenesis in mice\u2020","volume":"108","author":"Zhang","year":"2023","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.arr.2025.102807_bib115","article-title":"Single-cell analysis of ovarian myeloid cells identifies aging associated changes in macrophages and signaling dynamics","author":"Zhang","year":"2024","journal-title":"bioRxiv"},{"key":"10.1016\/j.arr.2025.102807_bib116","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1093\/biolre\/ioad080","article-title":"Follicular development and ovary aging: single-cell studies","volume":"109","author":"Zhao","year":"2023","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.arr.2025.102807_bib117","article-title":"Single-cell atlas of human ovaries reveals the role of the pyroptotic macrophage in ovarian aging","volume":"11","author":"Zhou","year":"2024","journal-title":"Adv. Sci. (Weinh. )"},{"key":"10.1016\/j.arr.2025.102807_bib118","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1093\/gerona\/glad009","article-title":"Resveratrol alleviates inflammation and ER stress through SIRT1\/NRF2 to delay ovarian aging in a short-lived fish","volume":"78","author":"Zhu","year":"2023","journal-title":"J. Gerontol. A Biol. Sci. Med Sci."}],"container-title":["Ageing Research Reviews"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1568163725001539?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1568163725001539?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T10:37:23Z","timestamp":1788950243000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1568163725001539"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":118,"alternative-id":["S1568163725001539"],"URL":"https:\/\/doi.org\/10.1016\/j.arr.2025.102807","relation":{},"ISSN":["1568-1637"],"issn-type":[{"value":"1568-1637","type":"print"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Ovarian aging at single-cell resolution: Current paradigms and perspectives","name":"articletitle","label":"Article Title"},{"value":"Ageing Research Reviews","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.arr.2025.102807","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"102807"}}