{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:20:38Z","timestamp":1790806838226,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T00:00:00Z","timestamp":1573084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T00:00:00Z","timestamp":1573084800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T00:00:00Z","timestamp":1573084800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T00:00:00Z","timestamp":1573084800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100007307","name":"Arthrex","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007307","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["arthroscopyjournal.org","clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Arthroscopy"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To identify an effective, nonenzymatic method for maximizing the yield of subacromial bursa\u2010derived nucleated cells for augmenting rotator cuff repair.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Subacromial bursa (minimum 0.2\u00a0g) was collected prospectively over the supraspinatus from patients (n\u00a0= 7) with at least one full\u2010thickness tendon tear undergoing arthroscopic primary rotator cuff repair. Samples were processed and analyzed prospectively using 4 different methods: (1) mechanical digestion with scissors (chopping), (2) collagenase digestion, (3) mechanical digestion with a tissue homogenizer, and (4) whole tissue with minimal manipulation. Tissue from each method were plated and cultured in a low oxygen tension, humidified incubator for 7\u00a0days. Following incubation, cellularity was assessed with nucleated cell count using a Coulter Counter. Flow cytometry was performed on the non\u2010enzymatic method that demonstrated the greatest cell count to confirm the presence of mesenchymal stem cells (MSCs). The Kruskal\u2013Wallis H test and post hoc Dunn's test were used for statistical analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Following incubation, mean nucleated cell counts (cells\/mL) were (1) 102,681 \u00b1 73,249 for chopping, (2) 76,190 \u00b1 66,275 for collagenase, (3) 31,686 \u00b1 29,234 for homogenization, and (4) 11,162 \u00b1 4016 for whole tissue. There was no significant difference between chopping and collagenase (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0= .45) or between homogenization and collagenase (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0= .52). Both chopping (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0= .003) and collagenase (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0= .03) produced significantly more cells when compared with whole tissue. Flow cytometry confirmed the presence of MSC markers on samples processed by chopping.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Mechanical isolation of subacromial bursa\u2010derived cells using a chopping technique demonstrated similar nucleated cell count compared with collagenase, along with the confirmed presence of MSCs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Relevance<\/jats:title>\n                    <jats:p>This study demonstrated a nonenzymatic, mechanical method for isolating subacromial bursa\u2010derived cells to potentially augment rotator cuff repair. Further clinical studies are required to assess its possible advent in the tendon\u2013bone healing process.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1016\/j.arthro.2019.07.024","type":"journal-article","created":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T09:07:00Z","timestamp":1573117620000},"page":"80-85","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":50,"title":["Comparison of Preparation Techniques for Isolating Subacromial Bursa\u2010Derived Cells as a Potential Augment for Rotator Cuff Repair"],"prefix":"10.1002","volume":"36","author":[{"given":"Daichi","family":"Morikawa","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery University of Connecticut Farmington Connecticut U.S.A."},{"name":"Department of Orthopaedic Surgery Juntendo University Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukas N.","family":"Muench","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery University of Connecticut Farmington Connecticut U.S.A."},{"name":"Department of Orthopaedic Sports Medicine Technical University of Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua B.","family":"Baldino","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery University of Connecticut Farmington Connecticut U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cameron","family":"Kia","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery University of Connecticut Farmington Connecticut U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremiah","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery University of Connecticut Farmington Connecticut U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Otto","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery University of Connecticut Farmington Connecticut U.S.A."},{"name":"Department of Orthopaedic Sports Medicine Technical University of Munich Germany"},{"name":"Department of Trauma Hand and Reconstructive Surgery University of M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leo","family":"Pauzenberger","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery University of Connecticut Farmington Connecticut U.S.A."},{"name":"St. Vincent Shoulder &amp; Sports Clinic Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix","family":"Dyrna","sequence":"additional","affiliation":[{"name":"Department of Trauma Hand and Reconstructive Surgery University of M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Beth R.","family":"McCarthy","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery University of Connecticut Farmington Connecticut U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Augustus D.","family":"Mazzocca","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery University of Connecticut Farmington Connecticut 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