{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T18:11:24Z","timestamp":1777486284543,"version":"3.51.4"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T00:00:00Z","timestamp":1576540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T00:00:00Z","timestamp":1576540800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T00:00:00Z","timestamp":1576540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T00:00:00Z","timestamp":1576540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["arthroscopyjournal.org","clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Arthroscopy"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To determine whether (1) human leukocyte\u2010platelet\u2010rich plasma (L\u2010PRP) or (2) leukocyte\u2010platelet\u2010rich fibrin (L\u2010PRF) delivered on a hyaluronic acid (HA) scaffold at a bovine chondral defect, a simulated cartilage tear interface, in\u00a0vitro would improve tissue formation based on biomechanical, histologic, and biochemical measures.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>L\u2010PRF and L\u2010PRP were prepared from 3 healthy volunteer donors and delivered in conjunction with HA scaffolds to defects created in full\u2010thickness bovine cartilage plugs harvested from bovine femoral condyle and trochlea. Specimens were cultured in\u00a0vitro for up to 42 days. Treatment groups included an HA scaffold alone and scaffolds containing L\u2010PRF or L\u2010PRP. Cartilage repair was assessed using biomechanical testing, histology, DNA quantification, and measurement of sulfated glycosaminoglycan and collagen content at 28 and 42 days.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      L\u2010PRF elicited the greatest degree of defect filling and improvement in other histologic measures. L\u2010PRF\u2013treated specimens also had the greatest cellularity when compared with L\u2010PRP and control at day 28 (560.4 \u03bcg vs 191.4 \u03bcg vs 124.2 \u03bcg,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0= .15); at day 48, there remained a difference, although not significant, between L\u2010PRF versus L\u2010PRP (761.1 \u03bcg vs 589.3 \u03bcg,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0= .219) . L\u2010PRF had greater collagen deposition when compared with L\u2010PRP at day 42 (40.1 \u03bcg vs 16.3 \u03bcg,\n                      <jats:italic>P<\/jats:italic>\n                      &lt; .0001). L\u2010PRF had significantly greater maximum interfacial strength compared with the control at day 42 (10.92 N vs 0.66 N,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0= .015) but had no significant difference compared with L\u2010PRP (10.92 N vs 6.58 N,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0= .536). L\u2010PRP facilitated a greater amount of sulfated glycosaminoglycan production at day 42 when compared with L\u2010PRF (15.9 \u03bcg vs 4.3 \u03bcg,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0= .009).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Delivery of leukocyte\u2010rich platelet concentrates in conjunction with a HA scaffold may allow for improvements in cartilage healing through different pathways. L\u2010PRF was not superior to L\u2010PRP in its biomechanical strength, suggesting that both treatments may be effective in improving biomechanical strength of healing cartilage through different pathways.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Relevance<\/jats:title>\n                    <jats:p>The delivery of platelet\u2010rich concentrates in conjunction HA scaffolds may augment healing cartilaginous injuries.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1016\/j.arthro.2019.12.004","type":"journal-article","created":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T19:43:24Z","timestamp":1576611804000},"page":"1431-1440","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"title":["Growth Factor Delivery to a Cartilage\u2010Cartilage Interface Using Platelet\u2010Rich Concentrates on a Hyaluronic Acid Scaffold"],"prefix":"10.1002","volume":"36","author":[{"given":"Ashley","family":"Titan","sequence":"first","affiliation":[{"name":"Department of Surgery Stanford University School of Medicine Palo Alto California U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Sch\u00e4r","sequence":"additional","affiliation":[{"name":"Orthopaedic Soft Tissue Research Program Sports Medicine and Shoulder Service the Department of Biomechanics Hospital for Special Surgery New York New York U.S.A."},{"name":"Department of Orthopaedic Surgery and Traumatology University of Bern Insel Hospital Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian","family":"Hutchinson","sequence":"additional","affiliation":[{"name":"Orthopaedic Soft Tissue Research Program Sports Medicine and Shoulder Service the Department of Biomechanics Hospital for Special Surgery New York New York U.S.A."},{"name":"Department of Orthopaedic Surgery University at Albany\u2013State University of New York Albany New York U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Demange","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tony","family":"Chen","sequence":"additional","affiliation":[{"name":"Orthopaedic Soft Tissue Research Program Sports Medicine and Shoulder Service the Department of Biomechanics Hospital for Special Surgery New York New York U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott","family":"Rodeo","sequence":"additional","affiliation":[{"name":"Orthopaedic Soft Tissue Research Program Sports Medicine and Shoulder Service the Department of Biomechanics Hospital for Special Surgery New York New York 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