{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T00:54:39Z","timestamp":1777078479782,"version":"3.51.4"},"reference-count":34,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["22-14-00028"],"award-info":[{"award-number":["22-14-00028"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["22-74-10008"],"award-info":[{"award-number":["22-74-10008"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Arthropod Structure & Development"],"published-print":{"date-parts":[[2023,9]]},"DOI":"10.1016\/j.asd.2023.101299","type":"journal-article","created":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T22:52:20Z","timestamp":1693608740000},"page":"101299","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Multiscale head anatomy of Megaphragma (Hymenoptera: Trichogrammatidae)"],"prefix":"10.1016","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-0284-873X","authenticated-orcid":false,"given":"Inna A.","family":"Desyatirkina","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasia A.","family":"Makarova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Song","family":"Pang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. Shan","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harald","family":"Hess","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexey A.","family":"Polilov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.asd.2023.101299_bib2","doi-asserted-by":"crossref","DOI":"10.3732\/apps.1300090","article-title":"3D Plant Cell Architecture of Arabidopsis thaliana (Brassicaceae) using focused ion beam-scanning electron microscopy","volume":"2","author":"Bhawana","year":"2014","journal-title":"Appl. Plant Sci."},{"key":"10.1016\/j.asd.2023.101299_bib1","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.ode.2006.06.003","article-title":"Head anatomy of Xyelidae (Hexapoda: Hymenoptera) and phylogenetic implications","volume":"7","author":"Beutel","year":"2007","journal-title":"Org. Divers. Evol."},{"key":"10.1016\/j.asd.2023.101299_bib3","series-title":"Histology of Insects: the Textbook","author":"Chaika","year":"2017"},{"key":"10.1016\/j.asd.2023.101299_bib4","series-title":"Egg Parasitoids in Agroecosystems with Emphasis on Trichogramma","author":"Consoli","year":"2010"},{"issue":"1","key":"10.1016\/j.asd.2023.101299_bib5","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-022-24390-4","article-title":"The 3D ultrastructure of the chordotonal organs in the antenna of a microwasp remains complex although simplified","volume":"12","author":"Diakova","year":"2022","journal-title":"Sci. Rep."},{"key":"10.1016\/j.asd.2023.101299_bib6","series-title":"Lehrbuch der Entomologie","author":"Eidmann","year":"1970"},{"key":"10.1016\/j.asd.2023.101299_bib7","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1002\/cne.24358","article-title":"From two to three dimensions: the importance of the third dimension for evaluating the limits to neuronal miniaturization in insects","volume":"526","author":"Fischer","year":"2018","journal-title":"J.\u00a0Comp. Neurol."},{"key":"10.1016\/j.asd.2023.101299_bib8","series-title":"The Evolution of the Genome","first-page":"3","article-title":"Genome Size Evolution in Animals","author":"Gregory","year":"2005"},{"key":"10.1016\/j.asd.2023.101299_bib9","series-title":"Encyclopedia of Insects","first-page":"1011","article-title":"Chapter 256 - tracheal system","author":"Harrison","year":"2009"},{"issue":"4","key":"10.1016\/j.asd.2023.101299_bib10","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/j.neuron.2013.12.017","article-title":"A systematic nomenclature for the insect brain","volume":"81","author":"Ito","year":"2014","journal-title":"Neuron"},{"issue":"32","key":"10.1016\/j.asd.2023.101299_bib11","doi-asserted-by":"crossref","first-page":"13198","DOI":"10.1073\/pnas.0611544104","article-title":"Increase in tracheal investment with beetle size supports hypothesis of oxygen limitation on insect gigantism","volume":"104","author":"Kaiser","year":"2007","journal-title":"Proc. Natl.Acad. Sci. USA"},{"key":"10.1016\/j.asd.2023.101299_bib12","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1007\/s00441-019-03037-3","article-title":"Three-dimensional architecture of pericardial nephrocytes in Drosophila melanogaster revealed by FIB\/SEM tomography","volume":"378","author":"Kawasaki","year":"2019","journal-title":"Cell Tiss. Res."},{"key":"10.1016\/j.asd.2023.101299_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.asd.2022.101200","article-title":"Metamorphosis and denucleation of the brain in the miniature wasp Megaphragma viggianii (Hymenoptera: Trichogrammatidae)","volume":"70","author":"Makarova","year":"2022","journal-title":"Arthropod Struct. Dev."},{"key":"10.1016\/j.asd.2023.101299_bib14","doi-asserted-by":"crossref","DOI":"10.1242\/jeb.164954","article-title":"Of what use is connectomics? A personal perspective on the Drosophila connectome","volume":"221","author":"Meinertzhagen","year":"2018","journal-title":"J.\u00a0Exp. Biol."},{"key":"10.1016\/j.asd.2023.101299_bib15","first-page":"11","article-title":"3D reconstruction of cell nuclei in a full Drosophila brain","author":"Mu","year":"2021","journal-title":"bioRxiv"},{"issue":"3","key":"10.1016\/j.asd.2023.101299_bib16","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.zool.2007.07.010","article-title":"Genome size, cell size, and the evolution of enucleated erythrocytes in attenuate salamanders","volume":"111","author":"Mueller","year":"2008","journal-title":"Zoology"},{"key":"10.1016\/j.asd.2023.101299_bib34","first-page":"269","article-title":"Chapter 11- Methods of enhanced FIB-SEM sample preparation and image acquisition","volume":"177","author":"Pang","year":"2023"},{"issue":"3","key":"10.1016\/j.asd.2023.101299_bib17","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1163\/157075609X454935","article-title":"Cephalic salivary gland ultrastructure of worker and queen eusocial bees (Hymenoptera, Apidae)","volume":"59","author":"Poiani","year":"2009","journal-title":"Anim. Biol. Leiden"},{"key":"10.1016\/j.asd.2023.101299_bib18","doi-asserted-by":"crossref","first-page":"561","DOI":"10.3390\/insects13060561","article-title":"Revision of the World Species of Megaphragma Timberlake (Hymenoptera: Trichogrammatidae)","volume":"13","author":"Polaszek","year":"2022","journal-title":"Insects"},{"key":"10.1016\/j.asd.2023.101299_bib19","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.asd.2011.09.001","article-title":"The smallest insects evolve anucleate neurons","volume":"41","author":"Polilov","year":"2012","journal-title":"Arthropod Struct. Dev."},{"key":"10.1016\/j.asd.2023.101299_bib20","series-title":"At the Size Limit - Effects of Miniaturization in Insects","author":"Polilov","year":"2016"},{"key":"10.1016\/j.asd.2023.101299_bib21","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0175566","article-title":"Anatomy of adult Megaphragma (Hymenoptera: Trichogrammatidae), one of the smallest insects, and new insight into insect miniaturization","volume":"12","author":"Polilov","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.asd.2023.101299_bib23","doi-asserted-by":"crossref","first-page":"4717","DOI":"10.1038\/s41598-021-83936-0","article-title":"Protocol for preparation of heterogeneous biological samples for 3D electron microscopy: a case study for insects","volume":"11","author":"Polilov","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.asd.2023.101299_bib22","doi-asserted-by":"crossref","first-page":"43095","DOI":"10.1038\/srep43095","article-title":"The scaling and allometry of organ size associated with miniaturization in insects: a case study for Coleoptera and Hymenoptera","volume":"7","author":"Polilov","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.asd.2023.101299_bib24","first-page":"85","article-title":"The head anatomy of Protanilla lini (Hymenoptera: Formicidae: Leptanillinae), with a hypothesis of their mandibular movement","volume":"31","author":"Richter","year":"2021","journal-title":"Myrmecol. New"},{"key":"10.1016\/j.asd.2023.101299_bib25","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1590\/S1519-566X2003000400008","article-title":"Ultramorphology and histology of the Polistes versicolor (Oliver) (Vespidae) thorax salivary gland compared with other Hymenoptera","volume":"32","author":"Rocha","year":"2003","journal-title":"Neotrop. Entomol."},{"key":"10.1016\/j.asd.2023.101299_bib26","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1051\/apido:2003053","article-title":"Antennal glands in male bees: structures for sexual communication by pheromones?","volume":"34","author":"Romani","year":"2003","journal-title":"Apidologie"},{"issue":"12","key":"10.1016\/j.asd.2023.101299_bib27","doi-asserted-by":"crossref","first-page":"e0226485","DOI":"10.1371\/journal.pone.0226485","article-title":"A partial genome assembly of the miniature parasitoid wasp, Megaphragma amalphitanum","volume":"14","author":"Sharko","year":"2019","journal-title":"PLoS One"},{"key":"10.1016\/j.asd.2023.101299_bib28","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.micron.2006.12.001","article-title":"Nuclear alterations associated to programmed cell ceath in larval salivary glands of Apis mellifera (Hymenoptera: Apidae)","volume":"39","author":"Silva-Zacarin","year":"2008","journal-title":"Micron"},{"key":"10.1016\/j.asd.2023.101299_bib29","series-title":"Resh, V.H., Carde, R.T. (Eds), Encyclopedia of Insects","first-page":"897","article-title":"Salivary glands","author":"Walker","year":"2009"},{"issue":"2","key":"10.1016\/j.asd.2023.101299_bib30","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1111\/j.1365-3113.2010.00556.x","article-title":"On the head morphology of Grylloblattodea (Insecta) and the systematic position of the order, with a new nomenclature for the head muscles of Dicondylia","volume":"36","author":"Wipfler","year":"2011","journal-title":"Syst. Entomol."},{"key":"10.1016\/j.asd.2023.101299_bib31","doi-asserted-by":"crossref","first-page":"e25916","DOI":"10.7554\/eLife.25916","article-title":"Enhanced FIB-SEM systems for large-volume 3D imaging","volume":"6","author":"Xu","year":"2017","journal-title":"Elife"},{"key":"10.1016\/j.asd.2023.101299_bib32","first-page":"852863","article-title":"Enabling FIB-SEM systems for large volume connectomics and cell biology","author":"Xu","year":"2019","journal-title":"bioRxiv"},{"issue":"1","key":"10.1016\/j.asd.2023.101299_bib33","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.str.2020.10.003","article-title":"Serial cryoFIB\/SEM reveals cytoarchitectural disruptions in Leigh syndrome patient cells","volume":"29","author":"Zhu","year":"2021","journal-title":"Structure"}],"container-title":["Arthropod Structure & Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S146780392300066X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S146780392300066X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T03:09:14Z","timestamp":1761448154000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S146780392300066X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9]]},"references-count":34,"alternative-id":["S146780392300066X"],"URL":"https:\/\/doi.org\/10.1016\/j.asd.2023.101299","relation":{},"ISSN":["1467-8039"],"issn-type":[{"value":"1467-8039","type":"print"}],"subject":[],"published":{"date-parts":[[2023,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Multiscale head anatomy of Megaphragma (Hymenoptera: Trichogrammatidae)","name":"articletitle","label":"Article Title"},{"value":"Arthropod Structure & Development","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.asd.2023.101299","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"101299"}}