{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T01:33:56Z","timestamp":1768095236697,"version":"3.49.0"},"reference-count":93,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100009706","name":"EU","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009706","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Education","award":["CZ.1.05\/4.1.00\/16.0340"],"award-info":[{"award-number":["CZ.1.05\/4.1.00\/16.0340"]}]},{"DOI":"10.13039\/501100004726","name":"Ministry of Health","doi-asserted-by":"publisher","award":["00023736"],"award-info":[{"award-number":["00023736"]}],"id":[{"id":"10.13039\/501100004726","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochimica et Biophysica Acta (BBA) - General Subjects"],"published-print":{"date-parts":[[2019,1]]},"DOI":"10.1016\/j.bbagen.2018.10.016","type":"journal-article","created":{"date-parts":[[2018,10,28]],"date-time":"2018-10-28T17:15:22Z","timestamp":1540746922000},"page":"266-277","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"title":["Lifetime-based photoconversion of EGFP as a tool for FLIM"],"prefix":"10.1016","volume":"1863","author":[{"given":"Petr","family":"Herman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ale\u0161","family":"Holoubek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbora","family":"Brodska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbagen.2018.10.016_bb0005","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/0378-1119(92)90691-H","article-title":"Primary Structure of the Aequorea-Victoria Green-Fluorescent Protein","volume":"111","author":"Prasher","year":"1992","journal-title":"Gene"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0010","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1126\/science.8303295","article-title":"Green Fluorescent Protein as a Marker for Gene-Expression","volume":"263","author":"Chalfie","year":"1994","journal-title":"Science (New York, N.Y.)"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0015","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1152\/physrev.00038.2009","article-title":"Fluorescent Proteins and their applications in Imaging living Cells and Tissues","volume":"90","author":"Chudakov","year":"2010","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0020","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1039\/C3CS60171D","article-title":"Fluorescent proteins for live-cell imaging with super-resolution","volume":"43","author":"Nienhaus","year":"2014","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0025","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1038\/nrm3786","article-title":"Advances in whole-embryo imaging: a quantitative transition is underway","volume":"15","author":"Pantazis","year":"2014","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0030","doi-asserted-by":"crossref","first-page":"3552","DOI":"10.2174\/138161209789207006","article-title":"Recent advances of fluorescent technologies for drug discovery and development","volume":"15","author":"Chakraborty","year":"2009","journal-title":"Curr. Pharm. Des."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0035","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1038\/13657","article-title":"Fluorescent proteins from nonbioluminescent Anthozoa species","volume":"17","author":"Matz","year":"1999","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0040","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1247\/csf.27.343","article-title":"Green fluorescent protein-like proteins in reef Anthozoa animals","volume":"27","author":"Miyawaki","year":"2002","journal-title":"Cell Struct. Funct."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0045","doi-asserted-by":"crossref","first-page":"14091","DOI":"10.1073\/pnas.97.26.14091","article-title":"Cracks in the beta-can: fluorescent proteins from Anemonia sulcata (Anthozoa, Actinaria)","volume":"97","author":"Wiedenmann","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0050","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1021\/acs.chemrev.6b00238","article-title":"Photoinduced Chemistry in Fluorescent Proteins: Curse or Blessing?","volume":"117","author":"Acharya","year":"2017","journal-title":"Chem. Rev."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0055","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1016\/j.cbpa.2013.05.031","article-title":"Photoswitchable fluorescent proteins: ten years of colorful chemistry and exciting applications","volume":"17","author":"Zhou","year":"2013","journal-title":"Curr. Opin. Chem. Biol."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0060","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1002\/iub.1023","article-title":"Reversible photoswitching in fluorescent proteins: a mechanistic view","volume":"64","author":"Bourgeois","year":"2012","journal-title":"IUBMB Life"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0065","doi-asserted-by":"crossref","first-page":"4592","DOI":"10.1093\/nar\/24.22.4592","article-title":"Optimized codon usage and chromophore mutations provide enhanced sensitivity with the green fluorescent protein","volume":"24","author":"Yang","year":"1996","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0070","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1146\/annurev.biochem.67.1.509","article-title":"The green fluorescent protein","volume":"67","author":"Tsien","year":"1998","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0075","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1562\/0031-8655(2000)0710771TEGFPA2.0.CO2","article-title":"The enhanced green fluorescent protein as a tool for the analysis of protein dynamics and localization: local fluorescence study at the single-molecule level","volume":"71","author":"Cinelli","year":"2000","journal-title":"Photochem. Photobiol."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0080","doi-asserted-by":"crossref","first-page":"2782","DOI":"10.1016\/S0006-3495(97)78307-3","article-title":"Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy","volume":"73","author":"Patterson","year":"1997","journal-title":"Biophys. J."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0085","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0047132","article-title":"Crystal Structure of Enhanced Green Fluorescent Protein to 1.35 angstrom Resolution Reveals Alternative Conformations for Glu222","volume":"7","author":"Arpino","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0090","doi-asserted-by":"crossref","first-page":"4999","DOI":"10.1021\/jp003813i","article-title":"Excited-state dynamics in the enhanced green fluorescent protein mutant probed by picosecond time-resolved single photon counting spectroscopy","volume":"105","author":"Cotlet","year":"2001","journal-title":"J. Phys. Chem. B"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0095","doi-asserted-by":"crossref","first-page":"13573","DOI":"10.1073\/pnas.95.23.13573","article-title":"Dynamics of fluorescence fluctuations in green fluorescent protein observed by fluorescence correlation spectroscopy","volume":"95","author":"Haupts","year":"1998","journal-title":"P Natl Acad Sci USA"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0100","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1529\/biophysj.107.107128","article-title":"Singlet oxygen photosensitization by EGFP and its chromophore HBDI","volume":"94","author":"Jimenez-Banzo","year":"2008","journal-title":"Biophys. J."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0105","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.jsb.2011.02.004","article-title":"Stabilizing role of glutamic acid 222 in the structure of Enhanced Green Fluorescent Protein","volume":"174","author":"Royant","year":"2011","journal-title":"J. Struct. Biol."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0110","doi-asserted-by":"crossref","first-page":"14913","DOI":"10.1021\/bi048725t","article-title":"Comparative studies on the structure and stability of fluorescent proteins EGFP, zFP506, mRFP1, \"dimer2\", and DsRed1","volume":"43","author":"Stepanenko","year":"2004","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0115","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1023\/A:1015323606154","article-title":"The influence of solvent viscosity on the fluorescence decay and time-resolved anisotropy of green fluorescent protein","volume":"12","author":"Suhling","year":"2002","journal-title":"J. Fluoresc."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0120","doi-asserted-by":"crossref","first-page":"3589","DOI":"10.1016\/S0006-3495(02)75359-9","article-title":"Imaging the environment of green fluorescent protein","volume":"83","author":"Suhling","year":"2002","journal-title":"Biophys. J."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0125","doi-asserted-by":"crossref","DOI":"10.1117\/1.2937212","article-title":"Effect of refractive index on the fluorescence lifetime of green fluorescent protein","volume":"13","author":"Tregidgo","year":"2008","journal-title":"J. Biomed. Opt."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0130","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.tibs.2016.09.010","article-title":"The growing and Glowing Toolbox of Fluorescent and Photoactive Proteins","volume":"42","author":"Rodriguez","year":"2017","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0135","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1126\/science.1068539","article-title":"Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells","volume":"296","author":"Zacharias","year":"2002","journal-title":"Science (New York, N.Y.)"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0140","doi-asserted-by":"crossref","DOI":"10.1186\/1741-7007-6-13","article-title":"Hue-Shifted Monomeric Variants of Clavularia Cyan Fluorescent Protein: Identification of the Molecular Determinants of Color and Applications in Fluorescence Imaging","volume":"6","author":"Ai","year":"2008","journal-title":"BMC Biol."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0145","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S0091-679X(08)61946-9","article-title":"Understanding structure-function relationships in the Aequorea victoria green fluorescent protein","volume":"Vol 58","author":"Cubitt","year":"1999","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0150","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1038\/nbt1172","article-title":"Engineering and characterization of a superfolder green fluorescent protein","volume":"24","author":"Pedelacq","year":"2006","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0155","doi-asserted-by":"crossref","first-page":"4247","DOI":"10.1242\/jcs.005801","article-title":"Advances in fluorescent protein technology","volume":"120","author":"Shaner","year":"2007","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0160","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1038\/373663b0","article-title":"Improved Green Fluorescence","volume":"373","author":"Heim","year":"1995","journal-title":"Nature"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0165","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0378-1119(95)00685-0","article-title":"FACS-optimized mutants of the green fluorescent protein (GFP)","volume":"173","author":"Cormack","year":"1996","journal-title":"Gene"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0170","doi-asserted-by":"crossref","first-page":"13617","DOI":"10.1073\/pnas.93.24.13617","article-title":"Chemical nature of the light emitter of the Aequorea green fluorescent protein","volume":"93","author":"Niwa","year":"1996","journal-title":"P Natl Acad Sci USA"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0175","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1038\/35073068","article-title":"Studying protein dynamics in living cells","volume":"2","author":"Lippincott-Schwartz","year":"2001","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0180","doi-asserted-by":"crossref","first-page":"1900","DOI":"10.1016\/S0006-3495(97)78835-0","article-title":"Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion","volume":"72","author":"Swaminathan","year":"1997","journal-title":"Biophys. J."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0185","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0962-8924(98)01433-0","article-title":"Photobleaching GFP reveals protein dynamics inside live cells","volume":"9","author":"White","year":"1999","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0190","first-page":"S7","article-title":"Photobleaching and photoactivation: following protein dynamics in living cells","author":"Lippincott-Schwartz","year":"2003","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0195","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1017\/S0033583515000013","article-title":"Fluorescence recovery after photobleaching in material and life sciences: putting theory into practice","volume":"48","author":"Loren","year":"2015","journal-title":"Q. Rev. Biophys."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0200","doi-asserted-by":"crossref","first-page":"7085","DOI":"10.1021\/bi047881x","article-title":"Reversible photobleaching of enhanced green fluorescent proteins","volume":"44","author":"Sinnecker","year":"2005","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0205","doi-asserted-by":"crossref","unstructured":"W.E. Moerner, E.J.G. Peterman, S. Brasselet, S. Kummer, R.M. Dickson, Optical methods for exploring dynamics of single copies of green fluorescent protein, Cytometry, 36 (1999) 232-238, Doi (10.1002\/(Sici)1097-0320(19990701)36:3<232::Aid-Cyto13>3.0.Co;2-L).","DOI":"10.1002\/(SICI)1097-0320(19990701)36:3<232::AID-CYTO13>3.0.CO;2-L"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0210","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1016\/S0006-3495(98)77870-1","article-title":"Green fluorescent protein as a noninvasive intracellular pH indicator","volume":"74","author":"Kneen","year":"1998","journal-title":"Biophys. J."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0215","doi-asserted-by":"crossref","first-page":"6803","DOI":"10.1073\/pnas.95.12.6803","article-title":"Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins","volume":"95","author":"Llopis","year":"1998","journal-title":"P Natl Acad Sci USA"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0220","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1038\/nchembio.174","article-title":"Green fluorescent proteins are light-induced electron donors","volume":"5","author":"Bogdanov","year":"2009","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0225","article-title":"Green to Red Photoconversion of GFP for Protein Tracking In Vivo","volume":"5","author":"Sattarzadeh","year":"2015","journal-title":"Sci Rep-Uk"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0230","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1038\/nmeth.1664","article-title":"A photoswitchable orange-to-far-red fluorescent protein PSmOrange","volume":"8","author":"Subach","year":"2011","journal-title":"Nat Methods"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0235","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.cbpa.2014.04.010","article-title":"Chromophore chemistry of fluorescent proteins controlled by light","volume":"20","author":"Shcherbakova","year":"2014","journal-title":"Curr. Opin. Chem. Biol."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0240","doi-asserted-by":"crossref","DOI":"10.1117\/1.2937829","article-title":"Prolonged irradiation of enhanced cyan fluorescent protein or Cerulean can invalidate Forster resonance energy transfer measurements","volume":"13","author":"Hoffmann","year":"2008","journal-title":"J. Biomed. Opt."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0245","series-title":"Biomedical Photonics Handbook, Second Edition :Fundamentals, Devices, and Techniques","first-page":"353","article-title":"Lifetime-Based Imaging","author":"Herman","year":"2014"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0250","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0301-0104(01)00486-4","article-title":"Multiphoton molecular spectroscopy and excited-state dynamics of enhanced green fluorescent protein (EGFP): acid-base specificity","volume":"274","author":"Heikal","year":"2001","journal-title":"Chem. Phys."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0255","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1007\/s10895-009-0589-1","article-title":"Maximum entropy analysis of analytically simulated complex fluorescence decays","volume":"21","author":"Vecer","year":"2011","journal-title":"J. Fluoresc."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0260","series-title":"Principles of Fluorescence Spectroscopy","author":"Lakowicz","year":"2006"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0265","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1016\/S0968-0004(00)89099-4","article-title":"Understanding, improving and using green fluorescent proteins","volume":"20","author":"Cubitt","year":"1995","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0270","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1126\/science.273.5280.1392","article-title":"Crystal structure of the Aequorea victoria green fluorescent protein","volume":"273","author":"Ormo","year":"1996","journal-title":"Science (New York, N.Y.)"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0275","series-title":"Reversible Denaturation of Aequorea Green-Fluorescent Protein - Physical Separation and Characterization of the Renatured Protein, Biochemistry","first-page":"4535","volume":"21","author":"Ward","year":"1982"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0280","doi-asserted-by":"crossref","first-page":"6672","DOI":"10.1073\/pnas.0700059104","article-title":"Structural basis for reversible photobleaching of a green fluorescent protein homologue","volume":"104","author":"Henderson","year":"2007","journal-title":"P Natl Acad Sci USA"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0285","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1038\/nrm1741","article-title":"Photoactivatable fluorescent proteins","volume":"6","author":"Lukyanov","year":"2005","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0290","doi-asserted-by":"crossref","first-page":"2989","DOI":"10.1529\/biophysj.108.130146","article-title":"Generation of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy","volume":"95","author":"Stiel","year":"2008","journal-title":"Biophys. J."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0295","doi-asserted-by":"crossref","first-page":"18343","DOI":"10.1073\/pnas.0805949105","article-title":"Structural characterization of IrisFP, an optical highlighter undergoing multiple photo-induced transformations","volume":"105","author":"Adam","year":"2008","journal-title":"P Natl Acad Sci USA"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0300","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1002\/cphc.200800839","article-title":"Structure, Dynamics and Optical Properties of Fluorescent Proteins: Perspectives for Marker Development","volume":"10","author":"Nienhaus","year":"2009","journal-title":"ChemPhysChem"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0305","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.bbrc.2016.06.047","article-title":"The crystal structure of red fluorescent protein TagRFP-T reveals the mechanism of its superior photostability","volume":"477","author":"Liu","year":"2016","journal-title":"Biochem Bioph Res Co"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0310","first-page":"63","article-title":"Viscosity Dependence of Fluorescent Quantum yields of some Dye Systems","volume":"75","author":"Forster","year":"1971","journal-title":"Z Phys Chem Neue Fol"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0315","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/s10895-005-2523-5","article-title":"Effects of Refractive Index and Viscosity on Fluorescence and Anisotropy Decays of Enhanced Cyan and Yellow Fluorescent Proteins","volume":"15","author":"Borst","year":"2005","journal-title":"J. Fluoresc."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0320","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1063\/1.1733166","article-title":"Relationship between Absorption Intensity and Fluorescence Lifetime of Molecules","volume":"37","author":"Strickler","year":"1962","journal-title":"J. Chem. Phys."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0325","doi-asserted-by":"crossref","first-page":"8362","DOI":"10.1073\/pnas.93.16.8362","article-title":"Ultra-fast excited state dynamics in green fluorescent protein: Multiple states and proton transfer","volume":"93","author":"Chattoraj","year":"1996","journal-title":"P Natl Acad Sci USA"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0330","doi-asserted-by":"crossref","first-page":"20536","DOI":"10.1039\/c3cp52820k","article-title":"On the optical absorption of the anionic GFP chromophore in vacuum, solution, and protein","volume":"15","author":"Petrone","year":"2013","journal-title":"Phys. Chem. Chem. Phys."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0335","doi-asserted-by":"crossref","first-page":"5296","DOI":"10.1021\/bi9902182","article-title":"Structural and spectral response of green fluorescent protein variants to changes in pH","volume":"38","author":"Elsliger","year":"1999","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0340","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/S0009-2614(98)01035-5","article-title":"Structure and rotation barriers for ground and excited states of the isolated chromophore of the green fluorescent protein","volume":"296","author":"Voityuk","year":"1998","journal-title":"Chem. Phys. Lett."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0345","series-title":"Modern Molecular Photochemistry","first-page":"978","author":"Turro","year":"1991"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0350","doi-asserted-by":"crossref","first-page":"2052","DOI":"10.1038\/sj.leu.2404839","article-title":"Aberrant cytoplasmic expression of C-terminal-truncated NPM leukaemic mutant is dictated by tryptophans loss and a new NES motif","volume":"21","author":"Falini","year":"2007","journal-title":"Leukemia"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0355","doi-asserted-by":"crossref","unstructured":"B. Falini, M.P. Martelli, NPM1-mutated AML: targeting by disassembling, Blood, 118 (2011) 2936\u20132938, https:\/\/doi.org\/10.1182\/blood-2011-07-366146; https:\/\/doi.org\/10.1182\/blood-2011-07-366146.","DOI":"10.1182\/blood-2011-07-366146"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0360","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1042\/BC20070117","article-title":"In nucleoli, the steady state of nucleolar proteins is leptomycin B-sensitive","volume":"100","author":"Muro","year":"2008","journal-title":"Biol. Cell."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0365","doi-asserted-by":"crossref","first-page":"1731","DOI":"10.1038\/leu.2009.124","article-title":"Altered nucleophosmin transport in acute myeloid leukaemia with mutated NPM1: molecular basis and clinical implications","volume":"23","author":"Falini","year":"2009","journal-title":"Leukemia"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0370","doi-asserted-by":"crossref","first-page":"12005","DOI":"10.1021\/bi7009037","article-title":"Ultrafast excited-state dynamics in the green fluorescent protein variant S65T\/H148D. 1. Mutagenesis and structural studies","volume":"46","author":"Shu","year":"2007","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0375","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1038\/nchem.2853","article-title":"Chromophore twisting in the excited state of a photoswitchable fluorescent protein captured by time-resolved serial femtosecond crystallography","volume":"10","author":"Coquelle","year":"2018","journal-title":"Nat. Chem."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0380","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/S0009-2614(97)88003-7","article-title":"Protonation effects on the chromophore of green fluorescent protein. Quantum chemical study of the absorption spectrum","volume":"272","author":"Voityuk","year":"1997","journal-title":"Chem. Phys. Lett."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0385","doi-asserted-by":"crossref","first-page":"2467","DOI":"10.1021\/jp5075219","article-title":"Role of Zwitterions in Kindling Fluorescent Protein Photochemistry","volume":"119","author":"Mironov","year":"2015","journal-title":"J. Phys. Chem. B"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0390","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1039\/b902341k","article-title":"Fluorescence lifetime images of green fluorescent protein in HeLa cells during TNF-alpha induced apoptosis","volume":"8","author":"Ito","year":"2009","journal-title":"Photochem. Photobiol. Sci."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0395","first-page":"155","article-title":"Sinha, Apoptosis: Molecular Mechanisms and Pathogenicity","volume":"8","author":"Rastogi","year":"2009","journal-title":"EXCLI J."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0400","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1007\/s12035-010-8125-5","article-title":"Where, O Death, is Thy Sting? A Brief Review of Apoptosis Biology","volume":"42","author":"Wyllie","year":"2010","journal-title":"Mol. Neurobiol."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0405","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.cplett.2007.06.034","article-title":"Fluorescence lifetime image of a single halobacterium","volume":"442","author":"Wang","year":"2007","journal-title":"Chem. Phys. Lett."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0410","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.1039\/c0pp00125b","article-title":"Singlet oxygen photosensitisation by GFP mutants: oxygen accessibility to the chromophore","volume":"9","author":"Jimenez-Banzo","year":"2010","journal-title":"Photochem. Photobiol. Sci."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0415","series-title":"Free Radicals in Biology and Medicine","author":"Halliwell","year":"2015"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0420","doi-asserted-by":"crossref","first-page":"5444","DOI":"10.1021\/acs.jpcb.5b02271","article-title":"A Light-Induced Reaction with Oxygen leads to Chromophore Decomposition and Irreversible Photobleaching in GFP-Type Proteins","volume":"119","author":"Grigorenko","year":"2015","journal-title":"J. Phys. Chem. B"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0425","doi-asserted-by":"crossref","first-page":"2839","DOI":"10.1016\/j.febslet.2009.07.041","article-title":"Structural basis for the phototoxicity of the fluorescent protein KillerRed","volume":"583","author":"Carpentier","year":"2009","journal-title":"FEBS Lett."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0430","series-title":"CRC Handbook of Chemistry and Physics, in","author":"Weast","year":"1986"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0435","series-title":"Bright GFP with Subnanosecond Fluorescence Lifetime, Sci Rep-Uk, 8","author":"Mamontova","year":"2018"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0440","doi-asserted-by":"crossref","first-page":"3775","DOI":"10.1021\/bi0622874","article-title":"Improved green and blue fluorescent proteins for expression in bacteria and mammalian cells","volume":"46","author":"Kremers","year":"2007","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0445","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.medpho.2014.12.001","article-title":"Fluorescence lifetime imaging (FLIM): basic concepts and some recent developments","volume":"27","author":"Suhling","year":"2015","journal-title":"Medical Photonics"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0450","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1002\/jcb.25420","article-title":"Low-Dose Actinomycin-D Induces Redistribution of Wild-Type and Mutated Nucleophosmin Followed by Cell Death in Leukemic Cells","volume":"117","author":"Brodska","year":"2016","journal-title":"J. Cell. Biochem."},{"key":"10.1016\/j.bbagen.2018.10.016_bb0455","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1111\/j.1600-0854.2010.01063.x","article-title":"Nucleolar Disruption Ensures Nuclear Accumulation of p21 upon DNA damage","volume":"11","author":"Abella","year":"2010","journal-title":"Traffic"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0460","series-title":"Standardization and Quality Assurance in Fluorescence Measurements I: Techniques","first-page":"233","article-title":"Evaluation of Time-Resolved Fluorescence Data: Typical Methods and Problems","author":"Patting","year":"2008"},{"key":"10.1016\/j.bbagen.2018.10.016_bb0465","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"}],"container-title":["Biochimica et Biophysica Acta (BBA) - General Subjects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304416518303374?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304416518303374?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T03:55:31Z","timestamp":1759636531000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304416518303374"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1]]},"references-count":93,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["S0304416518303374"],"URL":"https:\/\/doi.org\/10.1016\/j.bbagen.2018.10.016","relation":{},"ISSN":["0304-4165"],"issn-type":[{"value":"0304-4165","type":"print"}],"subject":[],"published":{"date-parts":[[2019,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Lifetime-based photoconversion of EGFP as a tool for FLIM","name":"articletitle","label":"Article Title"},{"value":"Biochimica et Biophysica Acta (BBA) - General Subjects","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbagen.2018.10.016","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}