{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T11:10:34Z","timestamp":1780917034447,"version":"3.54.1"},"reference-count":41,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T00:00:00Z","timestamp":1762387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20230213"],"award-info":[{"award-number":["BK20230213"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017950","name":"Shanghai Municipal Health Commission","doi-asserted-by":"publisher","award":["20214Y0118"],"award-info":[{"award-number":["20214Y0118"]}],"id":[{"id":"10.13039\/100017950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82322009"],"award-info":[{"award-number":["82322009"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101441"],"award-info":[{"award-number":["82101441"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007824","name":"Soochow University","doi-asserted-by":"publisher","award":["KJS2420"],"award-info":[{"award-number":["KJS2420"]}],"id":[{"id":"10.13039\/501100007824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010909","name":"Excellent Young Scientists Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010909","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochimica et Biophysica Acta (BBA) - General Subjects"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.bbagen.2025.130878","type":"journal-article","created":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T23:04:39Z","timestamp":1762038279000},"page":"130878","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Elevated cyclic hydrostatic pressure enhances the transfection activity of lipoplexes by activating clathrin-mediated endocytosis"],"prefix":"10.1016","volume":"1870","author":[{"given":"Weichen","family":"Zhan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongrui","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yizhuo","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwen","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanxiao","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxin","family":"Ge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangyun","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbagen.2025.130878_bb0005","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1038\/337387a0","article-title":"Cationic liposome-mediated transfection","volume":"337","author":"Felgner","year":"1989","journal-title":"Nature"},{"key":"10.1016\/j.bbagen.2025.130878_bb0010","doi-asserted-by":"crossref","first-page":"18021","DOI":"10.1074\/jbc.M111257200","article-title":"Lipoplex-mediated transfection of mammalian cells occurs through the cholesterol-dependent clathrin-mediated pathway of endocytosis","volume":"277","author":"Zuhorn","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbagen.2025.130878_bb0015","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.jconrel.2006.06.024","article-title":"Cationic lipids, lipoplexes and intracellular delivery of genes","volume":"116","author":"Wasungu","year":"2006","journal-title":"J. Controlled Release"},{"key":"10.1016\/j.bbagen.2025.130878_bb0020","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1016\/j.ejmech.2019.01.007","article-title":"Composition design and medical application of liposomes","volume":"164","author":"Li","year":"2019","journal-title":"Eur. J. Med. Chem."},{"key":"10.1016\/j.bbagen.2025.130878_bb0025","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1007\/s00249-012-0830-8","article-title":"Cationic liposome\/DNA complexes: from structure to interactions with cellular membranes","volume":"41","author":"Caracciolo","year":"2012","journal-title":"Eur. Biophys. J."},{"key":"10.1016\/j.bbagen.2025.130878_bb0030","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0006058","article-title":"Physico-chemical characteristics of lipoplexes influence cell uptake mechanisms and transfection efficacy","volume":"4","author":"Resina","year":"2009","journal-title":"PLoS One"},{"key":"10.1016\/j.bbagen.2025.130878_bb0035","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.plipres.2008.03.002","article-title":"Cationic liposomal lipids: From gene carriers to cell signaling","volume":"47","author":"Lonez","year":"2008","journal-title":"Prog. Lipid Res."},{"key":"10.1016\/j.bbagen.2025.130878_bb0040","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2021.701031","article-title":"An overview of methods and tools for transfection of eukaryotic cells in vitro","volume":"9","author":"Fus-Kujawa","year":"2021","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.bbagen.2025.130878_bb0045","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1006\/mthe.2000.0053","article-title":"Optimization of nonviral gene transfer of vascular smooth muscle cells in vitro and in vivo","volume":"1","author":"Armeanu","year":"2000","journal-title":"Mol. Ther."},{"key":"10.1016\/j.bbagen.2025.130878_bb0050","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1159\/000051040","article-title":"Liposome-mediated high-efficiency transfection of human endothelial cells","volume":"38","author":"Kaiser","year":"2001","journal-title":"J. Vasc. Res."},{"key":"10.1016\/j.bbagen.2025.130878_bb0055","first-page":"22","article-title":"Increasing transfection efficiency of lipoplexes by modulating complexation solution for transient gene expression","author":"Kim","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.bbagen.2025.130878_bb0060","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1038\/mt.2013.54","article-title":"Endocytosis of gene delivery vectors: from clathrin-dependent to lipid raft-mediated endocytosis","volume":"21","author":"El-Sayed","year":"2013","journal-title":"Mol. Ther."},{"key":"10.1016\/j.bbagen.2025.130878_bb0065","doi-asserted-by":"crossref","DOI":"10.1115\/1.4043947","article-title":"Regulation of cell behavior by hydrostatic pressure","volume":"71","author":"Liu","year":"2019","journal-title":"Appl. Mech. Rev."},{"key":"10.1016\/j.bbagen.2025.130878_bb0070","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.semcdb.2022.04.021","article-title":"Hydrostatic pressure as a driver of cell and tissue morphogenesis","volume":"131","author":"Chugh","year":"2022","journal-title":"Semin. Cell Dev. Biol."},{"key":"10.1016\/j.bbagen.2025.130878_bb0075","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1093\/bja\/ael110","article-title":"Monitoring the injured brain: Icp and cbf","volume":"97","author":"Steiner","year":"2006","journal-title":"Br. J. Anaesth."},{"key":"10.1016\/j.bbagen.2025.130878_bb0080","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.preteyeres.2016.07.002","article-title":"24-h monitoring devices and nyctohemeral rhythms of intraocular pressure","volume":"55","author":"Aptel","year":"2016","journal-title":"Prog. Retin. Eye Res."},{"key":"10.1016\/j.bbagen.2025.130878_bb0085","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/jhh.2008.81","article-title":"Postural changes in blood pressure and the prevalence of orthostatic hypotension among home-dwelling elderly aged 75 years or older","volume":"23","author":"Hiitola","year":"2009","journal-title":"J. Hum. Hypertens."},{"key":"10.1016\/j.bbagen.2025.130878_bb0090","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/j.biotechadv.2018.01.015","article-title":"High hydrostatic pressure in cancer immunotherapy and biomedicine","volume":"36","author":"Adkins","year":"2018","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.bbagen.2025.130878_bb0095","first-page":"1851","article-title":"Induction of tumor cell death by high hydrostatic pressure as a novel supporting technique in orthopedic surgery","volume":"10","author":"Diehl","year":"2003","journal-title":"Oncol. Rep."},{"key":"10.1016\/j.bbagen.2025.130878_bb0100","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1016\/j.ymthe.2005.03.038","article-title":"Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes","volume":"12","author":"Rejman","year":"2005","journal-title":"Mol. Ther."},{"key":"10.1016\/j.bbagen.2025.130878_bb0105","doi-asserted-by":"crossref","DOI":"10.15252\/embj.2021108719","article-title":"The hippo pathway drives the cellular response to hydrostatic pressure","volume":"41","author":"Park","year":"2022","journal-title":"EMBO J."},{"key":"10.1016\/j.bbagen.2025.130878_bb0110","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1161\/CIRCRESAHA.115.306361","article-title":"Vascular smooth muscle cells in atherosclerosis","volume":"118","author":"Bennett","year":"2016","journal-title":"Circ. Res."},{"key":"10.1016\/j.bbagen.2025.130878_bb0115","doi-asserted-by":"crossref","DOI":"10.1002\/cptx.21","article-title":"Quantification of lactate dehydrogenase for cell viability testing using cell lines and primary cultured astrocytes","volume":"72","author":"Kaja","year":"2017","journal-title":"Curr. Protoc. Toxicol."},{"key":"10.1016\/j.bbagen.2025.130878_bb0120","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.addr.2020.06.022","article-title":"Recent advancements in liposome technology","volume":"156","author":"Filipczak","year":"2020","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.bbagen.2025.130878_bb0125","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.jconrel.2007.08.022","article-title":"Lipoplex morphologies and their influences on transfection efficiency in gene delivery","volume":"123","author":"Ma","year":"2007","journal-title":"J. Controlled Release"},{"key":"10.1016\/j.bbagen.2025.130878_bb0130","first-page":"14","article-title":"Methods of liposomes preparation: formation and control factors of versatile nanocarriers for biomedical and nanomedicine application","author":"Lombardo","year":"2022","journal-title":"Pharmaceutics"},{"key":"10.1016\/j.bbagen.2025.130878_bb0135","doi-asserted-by":"crossref","first-page":"25876","DOI":"10.1038\/srep25876","article-title":"Liposome production by microfluidics: potential and limiting factors","volume":"6","author":"Carugo","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bbagen.2025.130878_bb0140","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.ultsonch.2004.06.006","article-title":"Ultrasound enhances liposome-mediated gene transfection","volume":"12","author":"Feril","year":"2005","journal-title":"Ultrason. Sonochem."},{"key":"10.1016\/j.bbagen.2025.130878_bb0145","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.bbamem.2004.02.007","article-title":"Transfection efficiency and cytotoxicity of cationic liposomes in salmonid cell lines of hepatocyte and macrophage origin","volume":"1663","author":"Romoren","year":"2004","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbagen.2025.130878_bb0150","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1016\/j.bpj.2024.06.007","article-title":"Fluidic shear stress alters clathrin dynamics and vesicle formation in endothelial cells","volume":"124","author":"Nawara","year":"2025","journal-title":"Biophys J."},{"key":"10.1016\/j.bbagen.2025.130878_bb0155","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1038\/nrm.2017.132","article-title":"Mechanisms of clathrin-mediated endocytosis","volume":"19","author":"Kaksonen","year":"2018","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.bbagen.2025.130878_bb0160","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10529-013-1325-0","article-title":"Transmembrane routes of cationic liposome-mediated gene delivery using human throat epidermis cancer cells","volume":"36","author":"Cui","year":"2014","journal-title":"Biotechnol. Lett."},{"key":"10.1016\/j.bbagen.2025.130878_bb0165","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1038\/nrm3151","article-title":"Molecular mechanism and physiological functions of clathrin-mediated endocytosis","volume":"12","author":"McMahon","year":"2011","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.bbagen.2025.130878_bb0170","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1038\/mt.2009.281","article-title":"The use of inhibitors to study endocytic pathways of gene carriers: optimization and pitfalls","volume":"18","author":"Vercauteren","year":"2010","journal-title":"Mol. Ther."},{"key":"10.1016\/j.bbagen.2025.130878_bb0175","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1083\/jcb.123.5.1107","article-title":"Mis-assembly of clathrin lattices on endosomes reveals a regulatory switch for coated pit formation","volume":"123","author":"Wang","year":"1993","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.bbagen.2025.130878_bb0180","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0045799","article-title":"Pitstop 2 is a potent inhibitor of clathrin-independent endocytosis","volume":"7","author":"Dutta","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.bbagen.2025.130878_bb0185","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/978-1-59745-178-9_2","article-title":"Pharmacological inhibition of endocytic pathways: is it specific enough to be useful?","volume":"440","author":"Ivanov","year":"2008","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.bbagen.2025.130878_bb0190","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.juro.2012.03.112","article-title":"Simulated bladder pressure stimulates human bladder smooth muscle cell proliferation via the pi3k\/sgk1 signaling pathway","volume":"188","author":"Chen","year":"2012","journal-title":"J. Urol."},{"key":"10.1016\/j.bbagen.2025.130878_bb0195","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2022.829316","article-title":"Ferroptosis due to cystathionine gamma lyase\/hydrogen sulfide downregulation under high hydrostatic pressure exacerbates vsmc dysfunction","volume":"10","author":"Jin","year":"2022","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.bbagen.2025.130878_bb0200","doi-asserted-by":"crossref","first-page":"4631","DOI":"10.1038\/s41598-024-54463-5","article-title":"Cellular transfection using rapid decrease in hydrostatic pressure","volume":"14","author":"Huang","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bbagen.2025.130878_bb0205","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1038\/s41556-024-01459-y","article-title":"Pressure sensing of lysosomes enables control of tfeb responses in macrophages","volume":"26","author":"Cai","year":"2024","journal-title":"Nat. Cell Biol."}],"container-title":["Biochimica et Biophysica Acta (BBA) - General Subjects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304416525001230?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304416525001230?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T10:27:42Z","timestamp":1780914462000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304416525001230"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":41,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["S0304416525001230"],"URL":"https:\/\/doi.org\/10.1016\/j.bbagen.2025.130878","relation":{},"ISSN":["0304-4165"],"issn-type":[{"value":"0304-4165","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Elevated cyclic hydrostatic pressure enhances the transfection activity of lipoplexes by activating clathrin-mediated endocytosis","name":"articletitle","label":"Article Title"},{"value":"Biochimica et Biophysica Acta (BBA) - General Subjects","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbagen.2025.130878","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"130878"}}