{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:48:47Z","timestamp":1792784927367,"version":"4.5.0"},"reference-count":35,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T00:00:00Z","timestamp":1412121600000},"content-version":"vor","delay-in-days":365,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"name":"Ontario Graduate Scholarship program"},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Heart and Stroke Foundation of Ontario","id":[{"id":"https:\/\/ror.org\/00qbpyp73","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochimica et Biophysica Acta (BBA) - Molecular Cell Research"],"published-print":{"date-parts":[[2013,10]]},"DOI":"10.1016\/j.bbamcr.2013.05.020","type":"journal-article","created":{"date-parts":[[2013,6,5]],"date-time":"2013-06-05T12:52:34Z","timestamp":1370436754000},"page":"2293-2301","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":126,"title":["Detection and quantification of endoplasmic reticulum stress in living cells using the fluorescent compound, Thioflavin T"],"prefix":"10.1016","volume":"1833","author":[{"given":"Daniel R.","family":"Beriault","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoff H.","family":"Werstuck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbamcr.2013.05.020_bb0005","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1091\/mbc.5.3.253","article-title":"How N-linked oligosaccharides affect glycoprotein folding in the endoplasmic reticulum","volume":"5","author":"Helenius","year":"1994","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0010","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1152\/physrev.1999.79.3.683","article-title":"Signal transduction from the endoplasmic reticulum to the cell nucleus","volume":"79","author":"Pahl","year":"1999","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0015","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1101\/gad.13.10.1211","article-title":"Stress signaling from the lumen of the endoplasmic reticulum: coordination of gene transcriptional and translational controls","volume":"13","author":"Kaufman","year":"1999","journal-title":"Genes Dev."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0020","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1152\/physrev.00015.2006","article-title":"Endoplasmic reticulum stress signaling in disease","volume":"86","author":"Marciniak","year":"2006","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0025","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1146\/annurev.pathmechdis.3.121806.151434","article-title":"Endoplasmic reticulum stress in disease pathogenesis","volume":"3","author":"Lin","year":"2008","journal-title":"Annu. Rev. Pathol. Mech. Dis."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0030","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/B978-0-12-385114-7.00004-0","article-title":"Measuring ER stress and the unfolded protein response using mammalian tissue culture system","volume":"490","author":"Oslowski","year":"2011","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0035","doi-asserted-by":"crossref","first-page":"e93","DOI":"10.1093\/nar\/gkl515","article-title":"Real-time detection and continuous monitoring of ER stress in vitro and in vivo by ES-TRAP: evidence for systemic, transient ER stress during endotoxemia","volume":"34","author":"Hiramatsu","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0040","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1038\/nm970","article-title":"A transgenic mouse model for monitoring endoplasmic reticulum stress","volume":"10","author":"Iwawaki","year":"2004","journal-title":"Nat. Med."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0045","doi-asserted-by":"crossref","first-page":"7448","DOI":"10.1128\/MCB.23.21.7448-7459.2003","article-title":"XBP-1 regulates a subset of endoplasmic reticulum resident chaperone genes in the unfolded protein response","volume":"23","author":"Lee","year":"2003","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0050","doi-asserted-by":"crossref","first-page":"27013","DOI":"10.1016\/S0021-9258(19)61473-0","article-title":"Activation of ATF6 and an ATF6 DNA binding site by the endoplasmic reticulum stress response","volume":"275","author":"Wang","year":"2000","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0055","first-page":"487","article-title":"Fluorescent stains, with special reference to amyloid and connective tissues","volume":"68","author":"Vassar","year":"1959","journal-title":"Arch. Pathol."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0060","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/0003-2697(89)90046-8","article-title":"Fluorometric determination of amyloid fibrils in vitro using the fluorescent dye, thioflavin T1","volume":"177","author":"Naiki","year":"1989","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0065","doi-asserted-by":"crossref","first-page":"4200","DOI":"10.1529\/biophysj.104.049700","article-title":"Detection and characterization of aggregates, prefibrillar amyloidogenic oligomers, and protofibrils using fluorescence spectroscopy","volume":"88","author":"Lindgren","year":"2005","journal-title":"Biophys. J."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0070","first-page":"299","article-title":"Histological staining of amyloid-beta in mouse brains","volume":"299","author":"Sigurdsson","year":"2005","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0075","doi-asserted-by":"crossref","first-page":"977","DOI":"10.2337\/diabetes.51.4.977","article-title":"Expression profiling of palmitate- and oleate-regulated genes provides novel insights into the effects of chronic lipid exposure on pancreatic beta-cell function","volume":"51","author":"Busch","year":"2002","journal-title":"Diabetes"},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0080","doi-asserted-by":"crossref","first-page":"12003","DOI":"10.1016\/S0021-9258(19)50300-3","article-title":"Transactivation of the grp78 promoter by Ca2+ depletion. A comparative analysis with A23187 and the endoplasmic reticulum Ca(2+)-ATPase inhibitor thapsigargin","volume":"268","author":"Li","year":"1993","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0085","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1038\/nm.2067","article-title":"Reducing endoplasmic reticulum stress through a macrophage lipid chaperone alleviates atherosclerosis","volume":"15","author":"Erbay","year":"2009","journal-title":"Nat. Med."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0090","doi-asserted-by":"crossref","first-page":"32200","DOI":"10.1074\/jbc.M005186200","article-title":"Folding and maturation of tyrosinase-related protein-1 are regulated by the post-translational formation of disulfide bonds and by N-glycan processing","volume":"275","author":"Negroiu","year":"2000","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0095","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1161\/01.ATV.0000154142.61859.94","article-title":"Glucosamine-induced endoplasmic reticulum stress promotes ApoB100 degradation: evidence for Grp78-mediated targeting to proteasomal degradation","volume":"25","author":"Qiu","year":"2005","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0100","doi-asserted-by":"crossref","first-page":"2726","DOI":"10.1194\/jlr.M600299-JLR200","article-title":"Disruption of endoplasmic reticulum structure and integrity in lipotoxic cell death","volume":"47","author":"Borradaile","year":"2006","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0105","doi-asserted-by":"crossref","first-page":"15920","DOI":"10.1073\/pnas.252341799","article-title":"Control of PERK eIF2alpha kinase activity by the endoplasmic reticulum stress-induced molecular chaperone P58IPK","volume":"99","author":"Yan","year":"2002","journal-title":"PNAS"},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0110","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/s11010-011-1205-7","article-title":"\u0392-adrenergic receptor stimulation induces endoplasmic reticulum stress in adult cardiac myocytes: role in apoptosis","volume":"364","author":"Dalal","year":"2012","journal-title":"Mol. Cell. Biochem."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0115","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1016\/S0047-6374(02)00098-2","article-title":"Amyloid fibril proteins","volume":"123","author":"Xing","year":"2002","journal-title":"Mech. Ageing Dev."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0120","doi-asserted-by":"crossref","first-page":"93","DOI":"10.2337\/diabetes.55.01.06.db05-0633","article-title":"Glucosamine-induced endoplasmic reticulum dysfunction is associated with accelerated atherosclerosis in a hyperglycemic mouse model","volume":"55","author":"Werstuck","year":"2006","journal-title":"Diabetes"},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0125","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.atherosclerosis.2011.07.108","article-title":"Glucosamine-supplementation promotes endoplasmic reticulum stress, hepatic steatosis and accelerated atherogenesis in apoE\u2212\/\u2212 mice","volume":"219","author":"Beriault","year":"2011","journal-title":"Atherosclerosis"},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0130","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1126\/science.1128294","article-title":"Chemical chaperones reduce ER stress and restore glucose homeostasis in a mouse model of type 2 diabetes","volume":"313","author":"Ozcan","year":"2006","journal-title":"Science"},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0135","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1016\/S0197-4580(96)00170-4","article-title":"Beta-amyloid deposition and other measures of neuropathology predict cognitive status in Alzheimer's disease","volume":"17","author":"Cummings","year":"1996","journal-title":"Neurobiol. Aging"},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0140","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1007\/978-1-61779-551-0_28","article-title":"Histological staining of amyloid and pre-amyloid peptides and proteins in mouse tissue","volume":"849","author":"Rajamohamedsait","year":"2012","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0145","doi-asserted-by":"crossref","first-page":"7856","DOI":"10.1021\/ja7109822","article-title":"Crystal structure of thioflavin T bound to the peripheral site of Torpedo californica acetylcholinesterase reveals how thioflavin T acts as a sensitive fluorescent reporter of ligand binding to the acylation site","volume":"130","author":"Harel","year":"2008","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0150","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.saa.2009.05.010","article-title":"Interactions of thioflavin T with serum albumins: spectroscopic analyses","volume":"74","author":"Sen","year":"2009","journal-title":"Spectrochim. Acta A Mol. Biomol. Spectrosc."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0155","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1021\/jz300412v","article-title":"Thioflavin T promotes A(1\u201340) amyloid fibrils formation","volume":"3","author":"D'Amico","year":"2012","journal-title":"J. Phys. Chem. Lett."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0160","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1038\/nature09873","article-title":"Amyloid-binding compounds maintain protein homeostasis during ageing and extend lifespan","volume":"472","author":"Alavez","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0165","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1021\/ja309588h","article-title":"Thioflavin T as an efficient inducer and selective fluorescent sensor for the human telomeric G-quadruplex DNA","volume":"135","author":"Mohanty","year":"2013","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0170","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/B978-0-12-385114-7.00008-8","article-title":"Detecting and quantitating physiological endoplasmic reticulum stress","volume":"490","author":"Qi","year":"2011","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.bbamcr.2013.05.020_bb0175","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1016\/j.ygyno.2012.11.024","article-title":"The endoplasmic reticulum stress marker, glucose-regulated protein-78 (GRP78) in visceral adipocytes predicts endometrial cancer progression and patient survival","volume":"128","author":"Matsuo","year":"2013","journal-title":"Gynecol. Oncol."}],"container-title":["Biochimica et Biophysica Acta (BBA) - Molecular Cell Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167488913002103?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167488913002103?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T22:11:49Z","timestamp":1645654309000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167488913002103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10]]},"references-count":35,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2013,10]]}},"alternative-id":["S0167488913002103"],"URL":"https:\/\/doi.org\/10.1016\/j.bbamcr.2013.05.020","relation":{},"ISSN":["0167-4889"],"issn-type":[{"value":"0167-4889","type":"print"}],"subject":[],"published":{"date-parts":[[2013,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Detection and quantification of endoplasmic reticulum stress in living cells using the fluorescent compound, Thioflavin T","name":"articletitle","label":"Article Title"},{"value":"Biochimica et Biophysica Acta (BBA) - Molecular Cell Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbamcr.2013.05.020","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}