{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:00:39Z","timestamp":1791352839890,"version":"4.3.1"},"reference-count":45,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,7,16]],"date-time":"2013-07-16T00:00:00Z","timestamp":1373932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochimica et Biophysica Acta (BBA) - Molecular Cell Research"],"published-print":{"date-parts":[[2013,12]]},"DOI":"10.1016\/j.bbamcr.2013.06.011","type":"journal-article","created":{"date-parts":[[2013,6,22]],"date-time":"2013-06-22T04:45:57Z","timestamp":1371876357000},"page":"2664-2672","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":49,"title":["cAMP inhibits migration, ruffling and paxillin accumulation in focal adhesions of pancreatic ductal adenocarcinoma cells: Effects of PKA and EPAC"],"prefix":"10.1016","volume":"1833","author":[{"given":"Alex","family":"Burdyga","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Conant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee","family":"Haynes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kees","family":"Jalink","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Sutton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Neoptolemos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eithne","family":"Costello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexei","family":"Tepikin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbamcr.2013.06.011_bb0005","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1124\/mol.112.080689","article-title":"A novel EPAC-specific inhibitor suppresses pancreatic cancer cell migration and invasion","volume":"83","author":"Almahariq","year":"2013","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0010","doi-asserted-by":"crossref","first-page":"2151","DOI":"10.1210\/en.2009-1139","article-title":"Functional roles of protein kinase A (PKA) and exchange protein directly activated by 3\u2032,5\u2032-cyclic adenosine 5\u2032-monophosphate (cAMP) 2 (EPAC2) in cAMP-mediated actions in adrenocortical cells","volume":"151","author":"Aumo","year":"2010","journal-title":"Endocrinology"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0015","doi-asserted-by":"crossref","first-page":"5607","DOI":"10.1158\/0008-5472.CAN-10-0056","article-title":"Exchange protein directly activated by cyclic AMP increases melanoma cell migration by a Ca2+-dependent mechanism","volume":"70","author":"Baljinnyam","year":"2010","journal-title":"Cancer Res."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0020","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1038\/nature09460","article-title":"The patterns and dynamics of genomic instability in metastatic pancreatic cancer","volume":"467","author":"Campbell","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0025","doi-asserted-by":"crossref","first-page":"R100","DOI":"10.1186\/gb-2006-7-10-r100","article-title":"Cell profiler: image analysis software for identifying and quantifying cell phenotypes","volume":"7","author":"Carpenter","year":"2006","journal-title":"Genome Biol."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0030","doi-asserted-by":"crossref","first-page":"13799","DOI":"10.1074\/jbc.M800555200","article-title":"cAMP inhibits cell migration by interfering with Rac-induced lamellipodium formation","volume":"283","author":"Chen","year":"2008","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0035","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1111\/j.1745-7270.2008.00438.x","article-title":"Epac and PKA: a tale of two intracellular cAMP receptors","volume":"40","author":"Cheng","year":"2008","journal-title":"Acta Biochim. Biophys. Sin. (Shanghai)"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0040","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1038\/nrgastro.2012.119","article-title":"New biomarkers and targets in pancreatic cancer and their application to treatment","volume":"9","author":"Costello","year":"2012","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0045","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1038\/nrgastro.2010.214","article-title":"Pancreatic cancer in 2010: new insights for early intervention and detection","volume":"8","author":"Costello","year":"2011","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0050","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1097\/MPA.0b013e3181c15963","article-title":"Phenotype and genotype of pancreatic cancer cell lines","volume":"39","author":"Deer","year":"2010","journal-title":"Pancreas"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0055","doi-asserted-by":"crossref","first-page":"35199","DOI":"10.1074\/jbc.M804448200","article-title":"Protein kinase A regulates 3-phosphatidylinositide dynamics during platelet-derived growth factor-induced membrane ruffling and chemotaxis","volume":"283","author":"Deming","year":"2008","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0060","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1039\/C0MB00079E","article-title":"Visualization of PKA activity in plasma membrane microdomains","volume":"7","author":"Depry","year":"2011","journal-title":"Mol. Biosyst."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0065","doi-asserted-by":"crossref","DOI":"10.1002\/0471142727.mb1815s91","article-title":"Visualization of kinase activity with FRET-based activity biosensors","volume":"Chapter 18","author":"Depry","year":"2010","journal-title":"Curr. Protoc. Mol. Biol."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0070","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1038\/ncb874","article-title":"A novel Epac-specific cAMP analogue demonstrates independent regulation of Rap1 and ERK","volume":"4","author":"Enserink","year":"2002","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0075","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1067\/msy.2003.220","article-title":"Inhibition of pancreatic cancer cell growth and induction of apoptosis with novel therapies directed against protein kinase A","volume":"134","author":"Farrow","year":"2003","journal-title":"Surgery"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0080","doi-asserted-by":"crossref","first-page":"2038","DOI":"10.1038\/sj.bjc.6605439","article-title":"Epac inhibits migration and proliferation of human prostate carcinoma cells","volume":"101","author":"Grandoch","year":"2009","journal-title":"Br. J. Cancer"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0085","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1038\/nature09198","article-title":"Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics","volume":"466","author":"Grashoff","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0090","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.cell.2011.02.013","article-title":"Hallmarks of cancer: the next generation","volume":"144","author":"Hanahan","year":"2011","journal-title":"Cell"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0095","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.bbamcr.2004.03.005","article-title":"Regulation of actin-based cell migration by cAMP\/PKA","volume":"1692","author":"Howe","year":"2004","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0100","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/j.ceb.2011.05.006","article-title":"Cross-talk between calcium and protein kinase A in the regulation of cell migration","volume":"23","author":"Howe","year":"2011","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0105","doi-asserted-by":"crossref","first-page":"14320","DOI":"10.1073\/pnas.0507072102","article-title":"Spatial regulation of the cAMP-dependent protein kinase during chemotactic cell migration","volume":"102","author":"Howe","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0110","doi-asserted-by":"crossref","first-page":"a005074","DOI":"10.1101\/cshperspect.a005074","article-title":"Integrins in cell migration","volume":"3","author":"Huttenlocher","year":"2011","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0115","first-page":"54","article-title":"Establishment and characterization of a human pancreatic cancer cell line (SUIT-2) producing carcinoembryonic antigen and carbohydrate antigen 19-9","volume":"78","author":"Iwamura","year":"1987","journal-title":"Jpn. J. Cancer Res."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0120","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.1083\/jcb.153.7.1427","article-title":"Differential dynamics of alpha 5 integrin, paxillin, and alpha-actinin during formation and disassembly of adhesions in migrating cells","volume":"153","author":"Laukaitis","year":"2001","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0125","doi-asserted-by":"crossref","first-page":"4930","DOI":"10.1091\/mbc.E08-06-0564","article-title":"Integrin-mediated protein kinase A activation at the leading edge of migrating cells","volume":"19","author":"Lim","year":"2008","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0130","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1016\/j.jmb.2005.09.089","article-title":"Improvement of a FRET-based indicator for cAMP by linker design and stabilization of donor\u2013acceptor interaction","volume":"354","author":"Lissandron","year":"2005","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0135","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1097\/MPA.0b013e318160748f","article-title":"The cAMP\/Epac1\/Rap1 pathway in pancreatic carcinoma","volume":"37","author":"Lorenz","year":"2008","journal-title":"Pancreas"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0140","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1016\/j.cellsig.2008.01.018","article-title":"cAMP-induced Epac\u2013Rap activation inhibits epithelial cell migration by modulating focal adhesion and leading edge dynamics","volume":"20","author":"Lyle","year":"2008","journal-title":"Cell. Signal."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0145","doi-asserted-by":"crossref","first-page":"e26552","DOI":"10.1371\/journal.pone.0026552","article-title":"Protein kinase A activity and anchoring are required for ovarian cancer cell migration and invasion","volume":"6","author":"McKenzie","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0150","doi-asserted-by":"crossref","first-page":"47895","DOI":"10.1074\/jbc.M107235200","article-title":"Protein kinase A regulates Rac and is required for the growth factor-stimulated migration of carcinoma cells","volume":"276","author":"O'Connor","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0155","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1083\/jcb.148.2.253","article-title":"RhoA function in lamellae formation and migration is regulated by the alpha6beta4 integrin and cAMP metabolism","volume":"148","author":"O'Connor","year":"2000","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0160","doi-asserted-by":"crossref","first-page":"5956","DOI":"10.1074\/jbc.M805606200","article-title":"Spatial distribution of protein kinase A activity during cell migration is mediated by A-kinase anchoring protein AKAP Lbc","volume":"284","author":"Paulucci-Holthauzen","year":"2009","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0165","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1038\/sj.embor.7400290","article-title":"Detecting cAMP-induced Epac activation by fluorescence resonance energy transfer: Epac as a novel cAMP indicator","volume":"5","author":"Ponsioen","year":"2004","journal-title":"EMBO Rep."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0170","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.cell.2011.11.025","article-title":"EMT and dissemination precede pancreatic tumor formation","volume":"148","author":"Rhim","year":"2012","journal-title":"Cell"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0175","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1016\/j.cell.2011.06.010","article-title":"Life at the leading edge","volume":"145","author":"Ridley","year":"2011","journal-title":"Cell"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0180","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0185","doi-asserted-by":"crossref","first-page":"11","DOI":"10.3322\/caac.21166","article-title":"Cancer statistics, 2013","volume":"63","author":"Siegel","year":"2013","journal-title":"CA Cancer J. Clin."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0190","doi-asserted-by":"crossref","first-page":"14789","DOI":"10.1021\/ja3034137","article-title":"A FRET-facilitated photoswitching using an orange fluorescent protein with the fast photoconversion kinetics","volume":"134","author":"Subach","year":"2012","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0195","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1038\/ncb2231","article-title":"Protein kinase A governs a RhoA\u2013RhoGDI protrusion\u2013retraction pacemaker in migrating cells","volume":"13","author":"Tkachenko","year":"2011","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0200","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.cell.2011.12.021","article-title":"Understanding metastasis in pancreatic cancer: a call for new clinical approaches","volume":"148","author":"Tuveson","year":"2012","journal-title":"Cell"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0205","doi-asserted-by":"crossref","first-page":"e1916","DOI":"10.1371\/journal.pone.0001916","article-title":"A comparison of donor\u2013acceptor pairs for genetically encoded FRET sensors: application to the Epac cAMP sensor as an example","volume":"3","author":"van der Krogt","year":"2008","journal-title":"PLoS One"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0210","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1038\/nature09515","article-title":"Distant metastasis occurs late during the genetic evolution of pancreatic cancer","volume":"467","author":"Yachida","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0215","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/71345","article-title":"A genetically encoded, fluorescent indicator for cyclic AMP in living cells","volume":"2","author":"Zaccolo","year":"2000","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0220","doi-asserted-by":"crossref","first-page":"1711","DOI":"10.1126\/science.1069982","article-title":"Discrete microdomains with high concentration of cAMP in stimulated rat neonatal cardiac myocytes","volume":"295","author":"Zaccolo","year":"2002","journal-title":"Science"},{"key":"10.1016\/j.bbamcr.2013.06.011_bb0225","doi-asserted-by":"crossref","first-page":"14997","DOI":"10.1073\/pnas.211566798","article-title":"Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering","volume":"98","author":"Zhang","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U. S. A."}],"container-title":["Biochimica et Biophysica Acta (BBA) - Molecular Cell Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167488913002346?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167488913002346?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T02:59:43Z","timestamp":1539572383000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167488913002346"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12]]},"references-count":45,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["S0167488913002346"],"URL":"https:\/\/doi.org\/10.1016\/j.bbamcr.2013.06.011","relation":{},"ISSN":["0167-4889"],"issn-type":[{"value":"0167-4889","type":"print"}],"subject":[],"published":{"date-parts":[[2013,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"cAMP inhibits migration, ruffling and paxillin accumulation in focal adhesions of pancreatic ductal adenocarcinoma cells: Effects of PKA and EPAC","name":"articletitle","label":"Article Title"},{"value":"Biochimica et Biophysica Acta (BBA) - Molecular Cell Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbamcr.2013.06.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}