{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T10:48:10Z","timestamp":1790160490172,"version":"4.1.0"},"reference-count":45,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2013,7,16]],"date-time":"2013-07-16T00:00:00Z","timestamp":1373932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"funder":[{"DOI":"10.13039\/100012371","name":"Faculty of Science and Engineering, University of Manchester","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012371","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochimica et Biophysica Acta (BBA) - Biomembranes"],"published-print":{"date-parts":[[2013,9]]},"DOI":"10.1016\/j.bbamem.2013.05.034","type":"journal-article","created":{"date-parts":[[2013,6,9]],"date-time":"2013-06-09T21:49:47Z","timestamp":1370814587000},"page":"2238-2246","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":66,"title":["Large scale production of the active human ASCT2 (SLC1A5) transporter in Pichia pastoris \u2014 functional and kinetic asymmetry revealed in proteoliposomes"],"prefix":"10.1016","volume":"1828","author":[{"given":"Piero","family":"Pingitore","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena","family":"Pochini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariafrancesca","family":"Scalise","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Galluccio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Hedfalk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cesare","family":"Indiveri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbamem.2013.05.034_bb0005","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1152\/physrev.1998.78.4.969","article-title":"Molecular biology of mammalian plasma membrane amino acid transporters","volume":"78","author":"Palacin","year":"1998","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0010","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.semcancer.2005.04.005","article-title":"Amino acid transporters ASCT2 and LAT1 in cancer: partners in crime?","volume":"15","author":"Fuchs","year":"2005","journal-title":"Semin. Cancer Biol."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0015","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1042\/BJ20101912","article-title":"The role of amino acid transporters in inherited and acquired diseases","volume":"436","author":"Broer","year":"2011","journal-title":"Biochem. J."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0020","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.bbamem.2003.12.001","article-title":"Reconstitution into liposomes and functional characterization of the carnitine transporter from renal cell plasma membrane","volume":"1661","author":"Pochini","year":"2004","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbamem.2013.05.034_bb0025","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/978-1-60761-447-0_5","article-title":"Studying amino acid transport using liposomes","volume":"606","author":"Indiveri","year":"2010","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0030","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1038\/nature03018","article-title":"Structure of a glutamate transporter homologue from Pyrococcus horikoshii","volume":"431","author":"Yernool","year":"2004","journal-title":"Nature"},{"key":"10.1016\/j.bbamem.2013.05.034_bb0035","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.bbabio.2010.10.014","article-title":"The structural basis of secondary active transport mechanisms","volume":"1807","author":"Forrest","year":"2011","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbamem.2013.05.034_bb0040","first-page":"95","article-title":"Apical transporters for neutral amino acids: physiology and pathophysiology","volume":"23","author":"Broer","year":"2008","journal-title":"Physiology (Bethesda)"},{"key":"10.1016\/j.bbamem.2013.05.034_bb0045","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.1016\/j.bcp.2010.06.032","article-title":"Inactivation by Hg2+ and methylmercury of the glutamine\/amino acid transporter (ASCT2) reconstituted in liposomes: prediction of the involvement of a CXXC motif by homology modelling","volume":"80","author":"Oppedisano","year":"2010","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0050","doi-asserted-by":"crossref","first-page":"2551","DOI":"10.1016\/j.bbamem.2011.05.011","article-title":"The B0AT1 amino acid transporter from rat kidney reconstituted in liposomes: kinetics and inactivation by methylmercury","volume":"1808","author":"Oppedisano","year":"2011","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbamem.2013.05.034_bb0055","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.pharmthera.2008.09.005","article-title":"Nutrient transporters in cancer: relevance to Warburg hypothesis and beyond","volume":"121","author":"Ganapathy","year":"2009","journal-title":"Pharmacol. Ther."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0060","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1006\/bbrc.1998.8434","article-title":"Sodium-dependent homo- and hetero-exchange of neutral amino acids mediated by the amino acid transporter ATB0","volume":"245","author":"Torres-Zamorano","year":"1998","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0065","doi-asserted-by":"crossref","first-page":"14883","DOI":"10.1074\/jbc.271.25.14883","article-title":"Cloning and functional characterization of a system ASC-like Na+-dependent neutral amino acid transporter","volume":"271","author":"Utsunomiya-Tate","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0070","doi-asserted-by":"crossref","first-page":"2184","DOI":"10.1046\/j.1471-4159.1999.02184.x","article-title":"The astroglial ASCT2 amino acid transporter as a mediator of glutamine efflux","volume":"73","author":"Broer","year":"1999","journal-title":"J. Neurochem."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0075","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1042\/bj20020982","article-title":"Identification of a plasma membrane glutamine transporter from the rat hepatoma cell line H4-IIE-C3","volume":"368","author":"Pollard","year":"2002","journal-title":"Biochem. J."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0080","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0197-0186(03)00123-2","article-title":"Glutamine uptake and expression of mRNA's of glutamine transporting proteins in mouse cerebellar and cerebral cortical astrocytes and neurons","volume":"44","author":"Dolinska","year":"2004","journal-title":"Neurochem. Int."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0085","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.bbamem.2004.09.007","article-title":"Reconstitution into liposomes of the glutamine\/amino acid transporter from renal cell plasma membrane: functional characterization, kinetics and activation by nucleotides","volume":"1667","author":"Oppedisano","year":"2004","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbamem.2013.05.034_bb0090","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.bbamem.2006.09.002","article-title":"The glutamine\/amino acid transporter (ASCT2) reconstituted in liposomes: transport mechanism, regulation by ATP and characterization of the glutamine\/glutamate antiport","volume":"1768","author":"Oppedisano","year":"2007","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbamem.2013.05.034_bb0095","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.taap.2012.09.011","article-title":"Inactivation of the glutamine\/amino acid transporter ASCT2 by 1,2,3-dithiazoles: proteoliposomes as a tool to gain insights in the molecular mechanism of action and of antitumor activity","volume":"265","author":"Oppedisano","year":"2012","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0100","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1093\/nar\/15.3.1281","article-title":"The codon adaptation Index\u2014a measure of directional synonymous codon usage bias, and its potential applications","volume":"15","author":"Sharp","year":"1987","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0105","doi-asserted-by":"crossref","first-page":"398","DOI":"10.3109\/09687688.2011.602219","article-title":"Improving recombinant eukaryotic membrane protein yields in Pichia pastoris: the importance of codon optimization and clone selection","volume":"28","author":"Oberg","year":"2011","journal-title":"Mol. Membr. Biol."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0110","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.pep.2007.07.007","article-title":"Exceptional overproduction of a functional human membrane protein","volume":"56","author":"Nyblom","year":"2007","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0115","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1385\/0-89603-421-6:107","article-title":"High cell-density fermentation","volume":"103","author":"Stratton","year":"1998","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0120","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1002\/bit.10616","article-title":"Cyclic fed-batch culture for production of human serum albumin in Pichia pastoris","volume":"82","author":"Bushell","year":"2003","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0125","doi-asserted-by":"crossref","first-page":"31915","DOI":"10.1074\/jbc.M111.242677","article-title":"Glycosylation increases the thermostability of human aquaporin 10 protein","volume":"286","author":"Oberg","year":"2011","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0130","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1042\/BJ20110544","article-title":"Reconstitution in liposomes of the functionally active human OCTN1 (SLC22A4) transporter overexpressed in Escherichia coli","volume":"439","author":"Pochini","year":"2011","journal-title":"Biochem. J."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0135","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1007\/s10534-011-9479-5","article-title":"Effects of heavy metal cations on the mitochondrial ornithine\/citrulline transporter reconstituted in liposomes","volume":"24","author":"Tonazzi","year":"2011","journal-title":"Biometals"},{"key":"10.1016\/j.bbamem.2013.05.034_bb0140","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/0076-6879(95)60150-3","article-title":"Mitochondrial metabolite carrier proteins: purification, reconstitution, and transport studies","volume":"260","author":"Palmieri","year":"1995","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0145","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.pep.2006.09.002","article-title":"Over-expression in Escherichia coli, purification and characterization of isoform 2 of human FAD synthetase","volume":"52","author":"Galluccio","year":"2007","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0150","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1006\/jmbi.1993.1626","article-title":"Comparative protein modelling by satisfaction of spatial restraints","volume":"234","author":"Sali","year":"1993","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0155","doi-asserted-by":"crossref","first-page":"8597","DOI":"10.1073\/pnas.132266599","article-title":"Employing Escherichia coli to functionally express, purify, and characterize a human transporter","volume":"99","author":"Quick","year":"2002","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0160","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1007\/s12033-012-9586-8","article-title":"Strategies of bacterial over expression of membrane transporters relevant in human health: the successful case of the three members of OCTN subfamily","volume":"54","author":"Indiveri","year":"2012","journal-title":"Mol. Biotechnol."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0165","doi-asserted-by":"crossref","first-page":"4552","DOI":"10.1021\/bi800060w","article-title":"Cell free expression and functional reconstitution of eukaryotic drug transporters","volume":"47","author":"Keller","year":"2008","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbamem.2013.05.034_bb0170","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.pep.2012.10.003","article-title":"Expression of human heteromeric amino acid transporters in the yeast Pichia pastoris","volume":"87","author":"Costa","year":"2013","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0175","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s12033-011-9406-6","article-title":"Over-expression in E. coli and purification of the human OCTN2 transport protein","volume":"50","author":"Galluccio","year":"2012","journal-title":"Mol. Biotechnol."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0180","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.bbrc.2012.04.105","article-title":"Over-expression in Escherichia coli, purification and reconstitution in liposomes of the third member of the OCTN sub-family: the mouse carnitine transporter OCTN3","volume":"422","author":"Scalise","year":"2012","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0185","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1046\/j.1471-4159.2003.01981.x","article-title":"Glutamine efflux from astrocytes is mediated by multiple pathways","volume":"87","author":"Deitmer","year":"2003","journal-title":"J. Neurochem."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0190","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1046\/j.1471-4159.2001.00322.x","article-title":"Transfer of glutamine between astrocytes and neurons","volume":"77","author":"Broer","year":"2001","journal-title":"J. Neurochem."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0195","doi-asserted-by":"crossref","first-page":"27991","DOI":"10.1074\/jbc.271.45.27991","article-title":"ASCT-1 is a neutral amino acid exchanger with chloride channel activity","volume":"271","author":"Zerangue","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0200","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1016\/S0899-9007(02)00780-3","article-title":"Plasma amino acid levels with a note on membrane transport: characteristics, regulation, and metabolic significance","volume":"18","author":"Cynober","year":"2002","journal-title":"Nutrition"},{"key":"10.1016\/j.bbamem.2013.05.034_bb0205","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1038\/nature08616","article-title":"Transport mechanism of a bacterial homologue of glutamate transporters","volume":"462","author":"Reyes","year":"2009","journal-title":"Nature"},{"key":"10.1016\/j.bbamem.2013.05.034_bb0210","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.abb.2005.08.016","article-title":"Identification of the promoter elements involved in the stimulation of ASCT2 expression by glutamine availability in HepG2 cells and the probable involvement of FXR\/RXR dimers","volume":"443","author":"Bungard","year":"2005","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0215","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11010-008-9735-3","article-title":"Genomic regulation of intestinal amino acid transporters by aldosterone","volume":"313","author":"Amaral","year":"2008","journal-title":"Mol. Cell. Biochem."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0220","doi-asserted-by":"crossref","first-page":"2113","DOI":"10.1007\/s10620-007-0120-y","article-title":"In human entrocytes, GLN transport and ASCT2 surface expression induced by short-term EGF are MAPK, PI3K, and Rho-dependent","volume":"53","author":"Avissar","year":"2008","journal-title":"Dig. Dis. Sci."},{"key":"10.1016\/j.bbamem.2013.05.034_bb0225","article-title":"Control of glutamine metabolism by the tumor suppressor Rb","author":"Reynolds","year":"2013","journal-title":"Oncogene"}],"container-title":["Biochimica et Biophysica Acta (BBA) - Biomembranes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0005273613001879?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0005273613001879?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T13:48:20Z","timestamp":1762091300000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0005273613001879"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9]]},"references-count":45,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["S0005273613001879"],"URL":"https:\/\/doi.org\/10.1016\/j.bbamem.2013.05.034","relation":{},"ISSN":["0005-2736"],"issn-type":[{"value":"0005-2736","type":"print"}],"subject":[],"published":{"date-parts":[[2013,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Large scale production of the active human ASCT2 (SLC1A5) transporter in Pichia pastoris \u2014 functional and kinetic asymmetry revealed in proteoliposomes","name":"articletitle","label":"Article Title"},{"value":"Biochimica et Biophysica Acta (BBA) - Biomembranes","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbamem.2013.05.034","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}