{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:59:21Z","timestamp":1790729961898,"version":"4.1.0"},"reference-count":29,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2014,3,1]],"date-time":"2014-03-01T00:00:00Z","timestamp":1393632000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2014,3,1]],"date-time":"2014-03-01T00:00:00Z","timestamp":1393632000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2015,9,9]],"date-time":"2015-09-09T00:00:00Z","timestamp":1441756800000},"content-version":"vor","delay-in-days":557,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31170032"],"award-info":[{"award-number":["31170032"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National High Technology Research and Development Program of China","award":["2011AA040406"],"award-info":[{"award-number":["2011AA040406"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochimica et Biophysica Acta (BBA) - Biomembranes"],"published-print":{"date-parts":[[2014,3]]},"DOI":"10.1016\/j.bbamem.2013.12.002","type":"journal-article","created":{"date-parts":[[2013,12,12]],"date-time":"2013-12-12T01:31:31Z","timestamp":1386811891000},"page":"932-939","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"title":["Mutations adjacent to the end of transmembrane helices 6 and 7 independently affect drug efflux capacity of yeast ABC transporter Pdr5p"],"prefix":"10.1016","volume":"1838","author":[{"given":"Zhigang","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingkai","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxian","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongquan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuming","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjun","family":"Guan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbamem.2013.12.002_bb0005","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1126\/science.1159746","article-title":"Outwitting multidrug resistance to antifungals","volume":"321","author":"Monk","year":"2008","journal-title":"Science"},{"key":"10.1016\/j.bbamem.2013.12.002_bb0010","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1111\/j.1742-4658.2009.07485.x","article-title":"Multidrug efflux pumps: substrate selection in ATP-binding cassette multidrug efflux pumps\u2014first come, first served?","volume":"277","author":"Ernst","year":"2010","journal-title":"FEBS J."},{"key":"10.1016\/j.bbamem.2013.12.002_bb0015","first-page":"207","article-title":"The pleitropic drug ABC transporters from Saccharomyces cerevisiae","volume":"3","author":"Rogers","year":"2001","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"10.1016\/j.bbamem.2013.12.002_bb0020","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.jsb.2010.10.012","article-title":"Toward understanding the mechanism of action of the yeast multidrug resistance transporter Pdr5p: a molecular modeling study","volume":"173","author":"Rutledge","year":"2011","journal-title":"J. Struct. Biol."},{"key":"10.1016\/j.bbamem.2013.12.002_bb0025","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/j.bbapap.2008.12.004","article-title":"Molecular basis of multidrug transport by ABC transporters","volume":"1794","author":"Seeger","year":"2009","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbamem.2013.12.002_bb0030","doi-asserted-by":"crossref","first-page":"7011","DOI":"10.1128\/jb.179.22.7011-7015.1997","article-title":"Glutamate residues located within putative transmembrane helices are essential for TetA(P)-mediated tetracycline efflux","volume":"179","author":"Kennan","year":"1997","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.bbamem.2013.12.002_bb0035","doi-asserted-by":"crossref","first-page":"C1100","DOI":"10.1152\/ajpcell.00160.2010","article-title":"Transmembrane helices 1 and 6 of the human breast cancer resistance protein (BCRP\/ABCG2): identification of polar residues important for drug transport","volume":"299","author":"Ni","year":"2010","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"10.1016\/j.bbamem.2013.12.002_bb0040","doi-asserted-by":"crossref","first-page":"12325","DOI":"10.1074\/jbc.M311435200","article-title":"Identification of proline residues in the core cytoplasmic and transmembrane regions of multidrug resistance protein 1 (MRP1\/ABCC1) important for transport function, substrate specificity, and nucleotide interactions","volume":"279","author":"Koike","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbamem.2013.12.002_bb0045","doi-asserted-by":"crossref","first-page":"14132","DOI":"10.1021\/bi026443s","article-title":"A positively charged amino acid proximal to the C-terminus of TM17 of MRP1 is indispensable for GSH-dependent binding of substrates and for transport of LTC4","volume":"41","author":"Ren","year":"2002","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbamem.2013.12.002_bb0050","doi-asserted-by":"crossref","first-page":"35010","DOI":"10.1074\/jbc.M806446200","article-title":"Mutations define cross-talk between the N-terminal nucleotide-binding domain and transmembrane helix-2 of the yeast multidrug transporter Pdr5: possible conservation of a signaling interface for coupling ATP hydrolysis to drug transport","volume":"283","author":"Sauna","year":"2008","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbamem.2013.12.002_bb0055","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1046\/j.1365-2958.2000.01798.x","article-title":"The transmembrane domain 10 of the yeast Pdr5p ABC antifungal efflux pump determines both substrate specificity and inhibitor susceptibility","volume":"35","author":"Egner","year":"2000","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.bbamem.2013.12.002_bb0060","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1111\/j.1365-2443.2005.00847.x","article-title":"Mutational analysis of the yeast multidrug resistance ABC transporter Pdr5p with altered drug specificity","volume":"10","author":"Tutulan-Cunita","year":"2005","journal-title":"Genes Cells"},{"key":"10.1016\/j.bbamem.2013.12.002_bb0065","doi-asserted-by":"crossref","first-page":"5069","DOI":"10.1073\/pnas.0800191105","article-title":"A mutation of the H-loop selectively affects rhodamine transport by the yeast multidrug ABC transporter Pdr5","volume":"105","author":"Ernst","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.bbamem.2013.12.002_bb0070","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1111\/j.1365-2958.2006.05562.x","article-title":"Subcellular trafficking of the yeast plasma membrane ABC transporter, Pdr5, is impaired by a mutation in the N-terminal nucleotide-binding fold","volume":"63","author":"De Thozee","year":"2007","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.bbamem.2013.12.002_bb0075","doi-asserted-by":"crossref","first-page":"4440","DOI":"10.1021\/bi100394j","article-title":"The signaling interface of the yeast multidrug transporter Pdr5 adopts a cis conformation, and there are functional overlap and equivalence of the deviant and canonical Q-loop residues","volume":"49","author":"Ananthaswamy","year":"2010","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbamem.2013.12.002_bb0080","doi-asserted-by":"crossref","first-page":"e29520","DOI":"10.1371\/journal.pone.0029520","article-title":"A mutation in intracellular loop 4 affects the drug-efflux activity of the yeast multidrug resistance ABC transporter Pdr5p","volume":"7","author":"Guo","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.bbamem.2013.12.002_bb0085","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1016\/j.febslet.2007.01.073","article-title":"Structure of the multidrug ABC transporter Sav 1866 from Staphylococcus aureus in complex with AMP-PNP","volume":"581","author":"Dawson","year":"2007","journal-title":"FEBS Lett."},{"key":"10.1016\/j.bbamem.2013.12.002_bb0090","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1038\/nature05155","article-title":"Structure of a bacterial multidrug ABC transporter","volume":"443","author":"Dawson","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.bbamem.2013.12.002_bb0095","doi-asserted-by":"crossref","first-page":"1718","DOI":"10.1126\/science.1168750","article-title":"Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding","volume":"323","author":"Aller","year":"2009","journal-title":"Science"},{"key":"10.1016\/j.bbamem.2013.12.002_bb0100","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1091\/mbc.9.2.523","article-title":"Genetic separation of FK506 susceptibility and drug transport in the yeast Pdr5 ATP-binding cassette multidrug resistance transporter","volume":"9","author":"Egner","year":"1998","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.bbamem.2013.12.002_bb0105","doi-asserted-by":"crossref","first-page":"3337","DOI":"10.1021\/bi972680x","article-title":"Mutational analysis of the P-glycoprotein first intracellular loop and flanking transmembrane domains","volume":"37","author":"Kwan","year":"1998","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbamem.2013.12.002_bb0110","first-page":"107","article-title":"Yeast transformation by the LiAc\/SS Carrier DNA\/PEG method","volume":"313","author":"Gietz","year":"2006","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.bbamem.2013.12.002_bb0115","doi-asserted-by":"crossref","first-page":"13109","DOI":"10.1021\/bi701414f","article-title":"Complete inhibition of the Pdr5p multidrug efflux pump ATPase activity by its transport substrate clotrimazole suggests that GTP as well as ATP may be used as an energy source","volume":"46","author":"Golin","year":"2007","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbamem.2013.12.002_bb0120","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1107\/S0907444904007437","article-title":"The role of CAPS buffer in expanding the crystallization space of the nucleotide-binding domain of the ABC transporter haemolysin B from Escherichia coli","volume":"60","author":"Zaitseva","year":"2004","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.bbamem.2013.12.002_bb0125","doi-asserted-by":"crossref","first-page":"6474","DOI":"10.1021\/jp003919d","article-title":"Evaluation and reparametrization of the OPLS-AA force field for proteins via comparison with accurate quantum chemical calculations on peptides","volume":"105","author":"Kaminski","year":"2001","journal-title":"J. Phys. Chem. B"},{"key":"10.1016\/j.bbamem.2013.12.002_bb0130","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1021\/jm0306430","article-title":"Glide: a new approach for rapid, accurate docking and scoring. 1. Method and assessment of docking accuracy","volume":"47","author":"Friesner","year":"2004","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bbamem.2013.12.002_bb0135","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1021\/jm030644s","article-title":"Glide: a new approach for rapid, accurate docking and scoring. 2. Enrichment factors in database screening","volume":"47","author":"Halgren","year":"2004","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bbamem.2013.12.002_bb0140","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/0079-6107(72)90005-3","article-title":"Protein volume in solution","volume":"24","author":"Zamyatnin","year":"1972","journal-title":"Prog. Biophys. Mol. Biol."},{"key":"10.1016\/j.bbamem.2013.12.002_bb0145","doi-asserted-by":"crossref","first-page":"1302","DOI":"10.1002\/pro.5560060618","article-title":"Hydrophobicity regained","volume":"6","author":"Karplus","year":"1997","journal-title":"Protein Sci."}],"updated-by":[{"DOI":"10.1016\/j.bbamem.2014.02.020","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000}}],"container-title":["Biochimica et Biophysica Acta (BBA) - Biomembranes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0005273613004318?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0005273613004318?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T08:42:43Z","timestamp":1757752963000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0005273613004318"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,3]]}},"alternative-id":["S0005273613004318"],"URL":"https:\/\/doi.org\/10.1016\/j.bbamem.2013.12.002","relation":{"erratum":[{"id-type":"doi","id":"10.1016\/j.bbamem.2014.02.020","asserted-by":"object"}]},"ISSN":["0005-2736"],"issn-type":[{"value":"0005-2736","type":"print"}],"subject":[],"published":{"date-parts":[[2014,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Mutations adjacent to the end of transmembrane helices 6 and 7 independently affect drug efflux capacity of yeast ABC transporter Pdr5p","name":"articletitle","label":"Article Title"},{"value":"Biochimica et Biophysica Acta (BBA) - Biomembranes","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbamem.2013.12.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}