{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:43:05Z","timestamp":1791297785630,"version":"4.1.0"},"reference-count":49,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"vor","delay-in-days":365,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"name":"Virtual Institute VH-VI-403 (Helmholtz-Association)"},{"name":"Cluster of Excellence RESOLV","award":["EXC 1069, DFG"],"award-info":[{"award-number":["EXC 1069, DFG"]}]},{"name":"Biointerfaces program in technology and medicine (Helmholtz-Association)"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochimica et Biophysica Acta (BBA) - Biomembranes"],"published-print":{"date-parts":[[2016,5]]},"DOI":"10.1016\/j.bbamem.2015.12.022","type":"journal-article","created":{"date-parts":[[2015,12,28]],"date-time":"2015-12-28T17:45:55Z","timestamp":1451324755000},"page":"918-925","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":35,"title":["Small angle X-ray scattering as a high-throughput method to classify antimicrobial modes of action"],"prefix":"10.1016","volume":"1858","author":[{"given":"A.R.","family":"von Gundlach","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.M.","family":"Garamus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Gorniak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.A.","family":"Davies","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Reischl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Mikut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Hilpert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Rosenhahn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbamem.2015.12.022_bb0005","first-page":"28-28","article-title":"The end of an era?","volume":"6","author":"Hancock","year":"2006","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0010","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1038\/nrd2201","article-title":"Drugs for bad bugs: confronting the challenges of antibacterial discovery","volume":"6","author":"Payne","year":"2007","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0015","doi-asserted-by":"publisher","first-page":"5994","DOI":"10.1128\/AAC.01673-13","article-title":"Tools for characterizing bacterial protein synthesis inhibitors","volume":"57","author":"Orelle","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0020","doi-asserted-by":"publisher","first-page":"1837","DOI":"10.1039\/c3mb70027e","article-title":"Mechanism of action-based classification of antibiotics using high-content bacterial image analysis","volume":"9","author":"Peach","year":"2013","journal-title":"Mol. BioSyst."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0025","doi-asserted-by":"publisher","first-page":"720","DOI":"10.1038\/nchembio.643","article-title":"High-frequency transposition for determining antibacterial mode of action","volume":"7","author":"Wang","year":"2011","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0030","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1038\/nrmicro2333","article-title":"How antibiotics kill bacteria: from targets to networks","volume":"8","author":"Kohanski","year":"2010","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0035","series-title":"Microscopy: Science, Technology, Applications and Education","first-page":"307","article-title":"Morphological changes induced in bacteria as evaluated by electron microscopy","author":"D\u00edaz-Visurraga","year":"2010"},{"key":"10.1016\/j.bbamem.2015.12.022_bb0040","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1016\/j.jsb.2006.07.007","article-title":"Transmission electron microscopy of the bacterial nucleoid","volume":"156","author":"Eltsov","year":"2006","journal-title":"J. Struct. Biol."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0045","doi-asserted-by":"publisher","first-page":"8662","DOI":"10.1021\/cr4001429","article-title":"Role of RNA polymerase and transcription in the organization of the bacterial nucleoid","volume":"113","author":"Jin","year":"2013","journal-title":"Chem. Rev."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0050","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1128\/JB.115.3.1167-1178.1973","article-title":"Nucleoid condensation and cell division in Escherichia coli MX74T2 ts52 after inhibition of protein synthesis","volume":"115","author":"Zusman","year":"1973","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0055","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1038\/nrmicro1098","article-title":"Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?","volume":"3","author":"Brogden","year":"2005","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0060","doi-asserted-by":"publisher","first-page":"1198","DOI":"10.1038\/nmeth.2213","article-title":"Staining and embedding the whole mouse brain for electron microscopy","volume":"9","author":"Mikula","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.bbamem.2015.12.022_bb0065","doi-asserted-by":"publisher","first-page":"748","DOI":"10.1016\/j.sbi.2013.06.007","article-title":"Impact and progress in small and wide angle X-ray scattering (SAXS and WAXS)","volume":"23","author":"Graewert","year":"2013","journal-title":"Curr. Opin. Struct. Biol."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0070","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1146\/annurev-physchem-040412-110132","article-title":"Small-angle X-ray scattering on biological macromolecules and nanocomposites in solution","volume":"64","author":"Blanchet","year":"2013","journal-title":"Annu. Rev. Phys. Chem."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0075","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1038\/nature13715","article-title":"Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies","volume":"515","author":"Wright","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.bbamem.2015.12.022_bb0080","doi-asserted-by":"publisher","first-page":"594","DOI":"10.1016\/j.cell.2013.10.008","article-title":"An unusual topological structure of the HIV-1 Rev response element","volume":"155","author":"Fang","year":"2013","journal-title":"Cell"},{"key":"10.1016\/j.bbamem.2015.12.022_bb0085","doi-asserted-by":"publisher","first-page":"11243","DOI":"10.1039\/c4cp00710g","article-title":"A new interpretation of SAXS peaks in sulfonated poly(ether ether ketone) (sPEEK) membranes for fuel cells","volume":"16","author":"Mendil-Jakani","year":"2014","journal-title":"Phys. Chem. Chem. Phys."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0090","doi-asserted-by":"publisher","first-page":"4061","DOI":"10.1021\/bm401186z","article-title":"Hydrolytically degradable polymer micelles for drug delivery: a SAXS\/SANS kinetic study","volume":"14","author":"Filippov","year":"2013","journal-title":"Biomacromolecules"},{"key":"10.1016\/j.bbamem.2015.12.022_bb0095","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1016\/j.carbon.2013.06.065","article-title":"Characterization of carbon nanotube webs and yarns with small angle X-ray scattering: revealing the yarn twist and inter-nanotube interactions and alignment","volume":"63","author":"Dum\u00e9e","year":"2013","journal-title":"Carbon"},{"key":"10.1016\/j.bbamem.2015.12.022_bb0100","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058043","article-title":"Microfibril orientation dominates the microelastic properties of human bone tissue at the lamellar length scale","volume":"8","author":"Granke","year":"2013","journal-title":"PLoS ONE"},{"key":"10.1016\/j.bbamem.2015.12.022_bb0105","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090884","article-title":"Nano-scale morphology of melanosomes revealed by small-angle X-ray scattering","volume":"9","author":"Gorniak","year":"2014","journal-title":"PLoS ONE"},{"key":"10.1016\/j.bbamem.2015.12.022_bb0110","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1016\/j.radphyschem.2013.02.025","article-title":"Mapping transitions between healthy and pathological lesions in human breast tissues by diffraction enhanced imaging computed tomography (DEI-CT) and small angle X-ray scattering (SAXS)","volume":"95","author":"LC","year":"2014","journal-title":"Radiat. Phys. Chem."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0115","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1038\/nmeth.1353","article-title":"Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS)","volume":"6","author":"Hura","year":"2009","journal-title":"Nat. Methods"},{"key":"10.1016\/j.bbamem.2015.12.022_bb0120","doi-asserted-by":"publisher","first-page":"123701","DOI":"10.1063\/1.3664870","article-title":"A 24keV liquid-metal-jet X-ray source for biomedical applications","volume":"82","author":"Larsson","year":"2011","journal-title":"Rev. Sci. Instrum."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0125","doi-asserted-by":"publisher","first-page":"s223","DOI":"10.1107\/S0021889807002853","article-title":"ATSAS 2.1 \u2014 towards automated and web-supported small-angle scattering data analysis","volume":"40","author":"Petoukhov","year":"2007","journal-title":"J. Appl. Crystallogr."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0130","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1016\/j.nima.2012.06.008","article-title":"Automated acquisition and analysis of small angle X-ray scattering data","volume":"689","author":"Franke","year":"2012","journal-title":"Nucl. Instrum. Methods Phys. Res., Sect. A"},{"key":"10.1016\/j.bbamem.2015.12.022_bb0135","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1107\/S0021889899012388","article-title":"Preparation of silver behenate coatings to provide low- to mid-angle diffraction calibration","volume":"33","author":"Blanton","year":"2000","journal-title":"J Appl Crystallogr. International Union of Crystallography"},{"key":"10.1016\/j.bbamem.2015.12.022_bb0140","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1038\/nprot.2007.521","article-title":"Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances","volume":"3","author":"Wiegand","year":"2008","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0145","series-title":"Proc, DGBMT-Workshop Biosignalverarbeitung, Potsdam.","first-page":"109","article-title":"The open source Matlab toolbox Gait-CAD and its application to bioelectric signal processing","author":"Mikut","year":"2008"},{"key":"10.1016\/j.bbamem.2015.12.022_bb0150","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1002\/wics.101","article-title":"Principal component analysis","volume":"2","author":"Abdi","year":"2010","journal-title":"Wiley Interdiscip. Rev. Comput. Stat."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0155","doi-asserted-by":"publisher","first-page":"827","DOI":"10.1126\/science.1117230","article-title":"Structures of the bacterial ribosome at 3.5A resolution","volume":"310","author":"Schuwirth","year":"2005","journal-title":"Science"},{"key":"10.1016\/j.bbamem.2015.12.022_bb0160","doi-asserted-by":"publisher","first-page":"1982","DOI":"10.1093\/nar\/gkh512","article-title":"Fundamental structural units of the Escherichia coli nucleoid revealed by atomic force microscopy","volume":"32","author":"Kim","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0165","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1107\/S0021889895005292","article-title":"Approximations leading to a unified exponential\/power-law approach to small-angle scattering","volume":"28","author":"Beaucage","year":"1995","journal-title":"J. Appl. Crystallogr."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0170","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1107\/S0021889887087107","article-title":"Scattering from fractals","volume":"20","author":"Martin","year":"1987","journal-title":"J. Appl. Crystallogr."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0175","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1107\/S0021889807001409","article-title":"Small-angle scattering studies of macromolecular solutions","volume":"40","author":"Svergun","year":"2007","journal-title":"J. Appl. Crystallogr."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0180","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1107\/S0021889895011605","article-title":"Small-angle scattering from polymeric mass fractals of arbitrary mass-fractal dimension","volume":"29","author":"Beaucage","year":"1996","journal-title":"J. Appl. Crystallogr."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0185","doi-asserted-by":"publisher","first-page":"4159","DOI":"10.1088\/0031-9155\/50\/17\/017","article-title":"A preliminary study of breast cancer diagnosis using laboratory based small angle X-ray scattering","volume":"50","author":"Round","year":"2005","journal-title":"Phys. Med. Biol."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0190","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1016\/j.jsb.2006.03.022","article-title":"Shape and compaction of Escherichia coli nucleoids","volume":"156","author":"Zimmerman","year":"2006","journal-title":"J. Struct. Biol."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0195","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1128\/JB.162.3.960-971.1985","article-title":"Shape and fine structure of nucleoids observed on sections of ultrarapidly frozen and cryosubstituted bacteria","volume":"162","author":"Hobot","year":"1985","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0200","doi-asserted-by":"publisher","first-page":"638","DOI":"10.1021\/la9810713","article-title":"Particle size distribution inferred from small-angle X-ray scattering and transmission electron microscopy","volume":"15","author":"Rieker","year":"1999","journal-title":"Langmuir"},{"key":"10.1016\/j.bbamem.2015.12.022_bb0205","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1111\/j.1365-2443.2007.01125.x","article-title":"Transcription-coupled nucleoid architecture in bacteria","volume":"12","author":"Ohniwa","year":"2007","journal-title":"Genes Cells"},{"key":"10.1016\/j.bbamem.2015.12.022_bb0210","doi-asserted-by":"publisher","first-page":"4180","DOI":"10.1128\/JB.01707-08","article-title":"Active transcription of rRNA operons condenses the nucleoid in Escherichia coli: examining the effect of transcription on nucleoid structure in the absence of transertion","volume":"191","author":"Cabrera","year":"2009","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0215","series-title":"Modern Aspects of Small-Angle Scattering","first-page":"1","article-title":"Some fundamental concepts and techniques useful in small-angle scattering studies of disordered solids","author":"Schmidt","year":"1995"},{"key":"10.1016\/j.bbamem.2015.12.022_bb0220","doi-asserted-by":"publisher","first-page":"10111","DOI":"10.1021\/jp505809v","article-title":"Weak self-interactions of globular proteins studied by small-angle X-ray scattering and structure-based modeling","volume":"118","author":"Kaieda","year":"2014","journal-title":"J. Phys. Chem. B."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0225","doi-asserted-by":"publisher","first-page":"11342","DOI":"10.1074\/jbc.M114.557348","article-title":"Organization of ribosomes and nucleoids in Escherichia coli cells during growth and in quiescence","volume":"289","author":"Chai","year":"2014","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0230","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/j.celrep.2013.01.027","article-title":"The impact of aminoglycosides on the dynamics of translation elongation","volume":"3","author":"Tsai","year":"2013","journal-title":"Cell Rep."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0235","doi-asserted-by":"publisher","first-page":"393","DOI":"10.3109\/10409230903307311","article-title":"The A\u2013Z of bacterial translation inhibitors","volume":"44","author":"Wilson","year":"2009","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"key":"10.1016\/j.bbamem.2015.12.022_bb0240","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1016\/j.ijantimicag.2007.10.005","article-title":"Role of the cell envelope in the antibacterial activities of polymyxin B and polymyxin B nonapeptide against Escherichia coli","volume":"31","author":"Sahalan","year":"2008","journal-title":"Int. J. Antimicrob. Agents"},{"key":"10.1016\/j.bbamem.2015.12.022_bb0245","doi-asserted-by":"publisher","first-page":"1008","DOI":"10.1038\/nbt1113","article-title":"High-throughput generation of small antibacterial peptides with improved activity","volume":"23","author":"Hilpert","year":"2005","journal-title":"Nat. Biotechnol."}],"container-title":["Biochimica et Biophysica Acta (BBA) - Biomembranes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0005273615004289?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0005273615004289?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,9,13]],"date-time":"2020-09-13T08:09:27Z","timestamp":1599984567000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0005273615004289"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5]]},"references-count":49,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["S0005273615004289"],"URL":"https:\/\/doi.org\/10.1016\/j.bbamem.2015.12.022","relation":{},"ISSN":["0005-2736"],"issn-type":[{"value":"0005-2736","type":"print"}],"subject":[],"published":{"date-parts":[[2016,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Small angle X-ray scattering as a high-throughput method to classify antimicrobial modes of action","name":"articletitle","label":"Article Title"},{"value":"Biochimica et Biophysica Acta (BBA) - Biomembranes","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbamem.2015.12.022","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}