{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T09:09:12Z","timestamp":1778663352209,"version":"3.51.4"},"reference-count":39,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics"],"published-print":{"date-parts":[[2010,11]]},"DOI":"10.1016\/j.bbapap.2010.07.020","type":"journal-article","created":{"date-parts":[[2010,8,7]],"date-time":"2010-08-07T08:37:38Z","timestamp":1281170258000},"page":"2102-2110","source":"Crossref","is-referenced-by-count":7,"title":["Conformational and biochemical characterization of a rat epididymis-specific lipocalin 12 expressed in Escherichia coli"],"prefix":"10.1016","volume":"1804","author":[{"given":"Yu","family":"Peng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiafu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yihe","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenyun","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yonglian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donghai","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbapap.2010.07.020_bb0005","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0167-4838(00)00151-5","article-title":"Evolution of the lipocalin family as inferred from a protein sequence phylogeny","volume":"1482","author":"Gutierrez","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0010","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/S0167-4838(00)00164-3","article-title":"Immunocalins: a lipocalin subfamily that modulates immune and inflammatory responses","volume":"1482","author":"Logdberg","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0015","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0167-4838(00)00150-3","article-title":"Plasma retinol binding protein: structure and function of the prototypic lipocalin","volume":"1482","author":"Newcomer","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0020","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1016\/S0021-9258(19)85050-0","article-title":"Primary structure of rat brain prostaglandin D synthetase deduced from cDNA sequence","volume":"264","author":"Urade","year":"1989","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbapap.2010.07.020_bb0025","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/0014-5793(94)01078-1","article-title":"The lipocalin protein family: a role in cell regulation","volume":"354","author":"Flower","year":"1994","journal-title":"FEBS Lett."},{"issue":"Pt 1","key":"10.1016\/j.bbapap.2010.07.020_bb0030","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/bj3180001","article-title":"The lipocalin protein family: structure and function","volume":"318","author":"Flower","year":"1996","journal-title":"Biochem. J."},{"key":"10.1016\/j.bbapap.2010.07.020_bb0035","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.bbapap.2005.12.006","article-title":"Comparative ligand-binding analysis of ten human lipocalins","volume":"1764","author":"Breustedt","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0040","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1210\/en.2002-220932","article-title":"Identification, immunolocalization, regulation, and postnatal development of the lipocalin EP17 (epididymal protein of 17 kilodaltons) in the mouse and rat epididymis","volume":"144","author":"Fouchecourt","year":"2003","journal-title":"Endocrinology"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0045","doi-asserted-by":"crossref","first-page":"2971","DOI":"10.1210\/en.139.6.2971","article-title":"Molecular cloning and hormonal regulation of a murine epididymal retinoic acid-binding protein messenger ribonucleic acid","volume":"139","author":"Lareyre","year":"1998","journal-title":"Endocrinology"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0050","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1186\/1477-7827-1-112","article-title":"LCN6, a novel human epididymal lipocalin","volume":"1","author":"Hamil","year":"2003","journal-title":"Reprod. Biol. Endocrinol."},{"key":"10.1016\/j.bbapap.2010.07.020_bb0055","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.gene.2004.06.027","article-title":"Molecular evolution of epididymal lipocalin genes localized on mouse chromosome 2","volume":"339","author":"Suzuki","year":"2004","journal-title":"Gene"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0060","doi-asserted-by":"crossref","first-page":"2027","DOI":"10.1110\/ps.8.10.2027","article-title":"Protein and ligand adaptation in a retinoic acid binding protein","volume":"8","author":"Pattanayek","year":"1999","journal-title":"Protein Sci."},{"key":"10.1016\/j.bbapap.2010.07.020_bb0065","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.mce.2005.12.043","article-title":"Epididymis-specific promoter-driven gene targeting: a transcription factor which regulates epididymis-specific gene expression","volume":"250","author":"Suzuki","year":"2006","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.1016\/j.bbapap.2010.07.020_bb0070","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.pep.2009.07.001","article-title":"Soluble expression and characterization of a mouse epididymis-specific protein lipocalin6","volume":"69","author":"Guo","year":"2010","journal-title":"Protein Expr Purif"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0075","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1016\/S1097-2765(02)00708-6","article-title":"The neutrophil lipocalin NGAL is a bacteriostatic agent that interferes with siderophore-mediated iron acquisition","volume":"10","author":"Goetz","year":"2002","journal-title":"Mol. Cell"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0080","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1038\/nature03104","article-title":"Lipocalin 2 mediates an innate immune response to bacterial infection by sequestrating iron","volume":"432","author":"Flo","year":"2004","journal-title":"Nature"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0085","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.canlet.2009.05.027","article-title":"Neutrophil gelatinase-associated lipocalin (NGAL) in human neoplasias: a new protein enters the scene","volume":"288","author":"Bolignano","year":"2010","journal-title":"Cancer Lett"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0090","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/S0140-6736(05)74811-X","article-title":"Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery","volume":"365","author":"Mishra","year":"2005","journal-title":"Lancet"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0095","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/S0167-4838(00)00161-8","article-title":"Biochemical, structural, genetic, physiological, and pathophysiological features of lipocalin-type prostaglandin D synthase","volume":"1482","author":"Urade","year":"2000","journal-title":"Bba-Protein Struct M."},{"key":"10.1016\/j.bbapap.2010.07.020_bb0100","doi-asserted-by":"crossref","first-page":"4876","DOI":"10.1093\/nar\/25.24.4876","article-title":"The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools","volume":"25","author":"Thompson","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.bbapap.2010.07.020_bb0105","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/0263-7855(96)00009-4","article-title":"MOLMOL: a program for display and analysis of macromolecular structures","volume":"14","author":"Koradi","year":"1996","journal-title":"J. Mol. Graph"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0110","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1007\/BF00228148","article-title":"AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR","volume":"8","author":"Laskowski","year":"1996","journal-title":"J. Biomol. NMR"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0115","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/BF00197809","article-title":"NMRPipe: a multidimensional spectral processing system based on UNIX pipes","volume":"6","author":"Delaglio","year":"1995","journal-title":"J. Biomol. NMR"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0120","first-page":"254-254","article-title":"UCSF Sparky\u2014an NMR display, annotation and assignment tool","volume":"53","author":"Kneller","year":"1993","journal-title":"J. Cell. Biochem."},{"key":"10.1016\/j.bbapap.2010.07.020_bb0125","doi-asserted-by":"crossref","first-page":"1754","DOI":"10.1194\/jlr.M700103-JLR200","article-title":"Hydrophobic ligand binding properties of the human lipocalin apolipoprotein M","volume":"48","author":"Ahnstrom","year":"2007","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.bbapap.2010.07.020_bb0130","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/j.jplph.2005.12.007","article-title":"Lipocalins\u2014a family portrait","volume":"163","author":"Grzyb","year":"2006","journal-title":"J. Plant Physiol."},{"key":"10.1016\/j.bbapap.2010.07.020_bb0135","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1002\/1099-1352(200101\/02)14:1<79::AID-JMR521>3.0.CO;2-4","article-title":"Bacterially produced apolipoprotein D binds progesterone and arachidonic acid, but not bilirubin or E-3M2H","volume":"14","author":"Vogt","year":"2001","journal-title":"J. Mol. Recognit."},{"key":"10.1016\/j.bbapap.2010.07.020_bb0140","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.bbapap.2005.04.003","article-title":"Role of the disulphide bridge in folding, stability and function of porcine odorant binding protein: spectroscopic equilibrium studies on C63A\/C155A double mutant","volume":"1750","author":"Parisi","year":"2005","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0145","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1002\/prot.20905","article-title":"Structural and thermodynamic consequences of removal of a conserved disulfide bond from equine beta-lactoglobulin","volume":"63","author":"Yamada","year":"2006","journal-title":"Proteins"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0150","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1016\/j.biochi.2008.06.003","article-title":"Effects of removing a conserved disulfide bond on the biological characteristics of rat lipocalin-type prostaglandin D synthase","volume":"90","author":"Liu","year":"2008","journal-title":"Biochimie"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0155","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1002\/prot.21966","article-title":"Mutant bovine odorant-binding protein: temperature affects the protein stability and dynamics as revealed by infrared spectroscopy and molecular dynamics simulations","volume":"72","author":"Marabotti","year":"2008","journal-title":"Proteins"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0160","doi-asserted-by":"crossref","first-page":"2215","DOI":"10.1021\/bi9720888","article-title":"A conserved disulfide motif in human tear lipocalins influences ligand binding","volume":"37","author":"Glasgow","year":"1998","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0165","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/0092-8674(95)90200-7","article-title":"The Rxr heterodimers and orphan receptors","volume":"83","author":"Mangelsdorf","year":"1995","journal-title":"Cell"},{"key":"10.1016\/j.bbapap.2010.07.020_bb0170","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1095\/biolreprod48.2.235","article-title":"Endogenous retinoids in rat epididymal tissue and rat and human spermatozoa","volume":"48","author":"Pappas","year":"1993","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.bbapap.2010.07.020_bb0175","doi-asserted-by":"crossref","first-page":"185","DOI":"10.3109\/10408369209114600","article-title":"Physiological and clinical aspects of vitamin-A and its metabolites","volume":"29","author":"Goss","year":"1992","journal-title":"Crit. Rev. Cl Lab. Sci."},{"key":"10.1016\/j.bbapap.2010.07.020_bb0180","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1095\/biolreprod56.4.985","article-title":"Male infertility caused by epididymal dysfunction in transgenic mice expressing a dominant negative mutation of retinoic acid receptor alpha","volume":"56","author":"Costa","year":"1997","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.bbapap.2010.07.020_bb0185","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/0003-9861(91)90482-X","article-title":"Solubility of retinoids in water","volume":"287","author":"Szuts","year":"1991","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.bbapap.2010.07.020_bb0190","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1042\/bj3340155","article-title":"Expression, characterization and engineered specificity of rat epididymal retinoic acid-binding protein","volume":"334","author":"Sundaram","year":"1998","journal-title":"Biochem. J."},{"key":"10.1016\/j.bbapap.2010.07.020_bb0195","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1006\/abio.1996.0028","article-title":"A simple assay for intracellular lipid-binding proteins using displacement of 1-anilinonaphthalene 8-sulfonic acid","volume":"233","author":"Kane","year":"1996","journal-title":"Anal. Biochem."}],"container-title":["Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570963910002141?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570963910002141?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,11,4]],"date-time":"2021-11-04T00:22:07Z","timestamp":1635985327000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1570963910002141"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11]]},"references-count":39,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2010,11]]}},"alternative-id":["S1570963910002141"],"URL":"https:\/\/doi.org\/10.1016\/j.bbapap.2010.07.020","relation":{},"ISSN":["1570-9639"],"issn-type":[{"value":"1570-9639","type":"print"}],"subject":[],"published":{"date-parts":[[2010,11]]}}}