{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T08:38:46Z","timestamp":1772008726259,"version":"3.50.1"},"reference-count":33,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["JP17K19627"],"award-info":[{"award-number":["JP17K19627"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics"],"published-print":{"date-parts":[[2019,1]]},"DOI":"10.1016\/j.bbapap.2018.05.006","type":"journal-article","created":{"date-parts":[[2018,5,10]],"date-time":"2018-05-10T00:51:28Z","timestamp":1525913488000},"page":"76-81","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"title":["A rapid separation and characterization of mucins from mouse submandibular glands by supported molecular matrix electrophoresis"],"prefix":"10.1016","volume":"1867","author":[{"given":"Akihiko","family":"Kameyama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimi","family":"Yamakoshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsushi","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbapap.2018.05.006_bb0005","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.cocis.2005.11.001","article-title":"Mucin structure, aggregation, physiological functions and biomedical applications","volume":"11","author":"Bansil","year":"2006","journal-title":"Curr. Opin. Colloid Interface Sci."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0010","doi-asserted-by":"crossref","first-page":"874","DOI":"10.1038\/nrc2761","article-title":"Mucins in cancer: function, prognosis and therapy","volume":"9","author":"Kufe","year":"2009","journal-title":"Nat. Rev. Cancer"},{"key":"10.1016\/j.bbapap.2018.05.006_bb0015","first-page":"1","article-title":"Analysis of the plasma proteome using iTRAQ and TMT-based isobaric labeling","author":"Moulder","year":"2017","journal-title":"Mass Spectrom. Rev."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0020","article-title":"Plasma prefractionation methods for proteomic analysis and perspectives in clinical applications","volume":"11","author":"Chutipongtanate","year":"2017","journal-title":"Proteomics Clin. Appl."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0025","doi-asserted-by":"crossref","first-page":"1936","DOI":"10.1111\/febs.14014","article-title":"From phosphoproteins to phosphoproteomes: a historical account","volume":"284","author":"Venerando","year":"2017","journal-title":"FEBS J."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0030","doi-asserted-by":"crossref","DOI":"10.1002\/pmic.201600235","article-title":"Impact of phosphoproteomics in the translation of kinase-targeted therapies","volume":"17","author":"Casado","year":"2017","journal-title":"Proteomics"},{"key":"10.1016\/j.bbapap.2018.05.006_bb0035","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1586\/14789450.4.3.329","article-title":"Proteomic analysis of saliva for cancer diagnosis","volume":"4","author":"Streckfus","year":"2007","journal-title":"Expert Rev. Proteomics"},{"key":"10.1016\/j.bbapap.2018.05.006_bb0040","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/nrc1043","article-title":"E.F. Early detection: proteomic applications for the early detection of cancer","volume":"3","author":"Wulfkuhle","year":"2003","journal-title":"Nat. Rev. Cancer"},{"key":"10.1016\/j.bbapap.2018.05.006_bb0045","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1021\/pr8003776","article-title":"Proteomic identification of salivary biomarkers of type-2 diabetes","volume":"8","author":"Rao","year":"2009","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0050","doi-asserted-by":"crossref","first-page":"3588","DOI":"10.1002\/art.22954","article-title":"Salivary proteomic and genomic biomarkers for primary Sj\u00f6gren's syndrome","volume":"56","author":"Hu","year":"2007","journal-title":"Arthritis Rheum."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0055","doi-asserted-by":"crossref","first-page":"6802","DOI":"10.1038\/srep06802","article-title":"The early diagnosis and monitoring of squamous cell carcinoma via saliva metabolomics","volume":"4","author":"Wang","year":"2014","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0060","doi-asserted-by":"crossref","first-page":"10802","DOI":"10.1021\/ac3028307","article-title":"Development of isotope labeling LC-MS for human salivary metabolomics and application to profiling metabolome changes associated with mild cognitive impairment","volume":"84","author":"Zheng","year":"2012","journal-title":"Anal. Chem."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0065","first-page":"490531","article-title":"Glycosylation-based serum biomarkers for cancer diagnostics and prognostics","author":"Kirwan","year":"2015","journal-title":"Biomed. Res. Int."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0070","doi-asserted-by":"crossref","first-page":"3979","DOI":"10.1158\/1078-0432.CCR-05-2412","article-title":"Concomitant analysis of salivary tumor markers\u2014a new diagnostic tool for oral cancer","volume":"12","author":"Nagler","year":"2006","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0075","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1093\/glycob\/cwn022","article-title":"The O-linked glycosylation of secretory\/shed MUC1 from an advanced breast cancer patient's serum","volume":"18","author":"Stor","year":"2008","journal-title":"Glycobiology"},{"key":"10.1016\/j.bbapap.2018.05.006_bb0080","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1007\/s00535-016-1230-0","article-title":"Wisteria floribunda agglutinin-sialylated mucin core polypeptide 1 is a sensitive biomarker for biliary tract carcinoma and intrahepatic cholangiocarcinoma: a multicenter study","volume":"52","author":"Shoda","year":"2017","journal-title":"J. Gastroenterol."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0085","doi-asserted-by":"crossref","first-page":"7025","DOI":"10.18632\/oncotarget.12347","article-title":"Mucins and associated O-glycans based immunoprofile for stratification of colorectal polyps: clinical implication for improved colon surveillance","volume":"8","author":"Krishn","year":"2017","journal-title":"Oncotarget"},{"key":"10.1016\/j.bbapap.2018.05.006_bb0090","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/0003-9861(68)90301-9","article-title":"Purification and characterization of bovine and ovine submaxillary mucins","volume":"124","author":"Tettamanti","year":"1968","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0095","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/0003-9969(74)90129-0","article-title":"Isolation, purification and chemical characterization of mucins from human submandibular glands","volume":"19","author":"Oemrawsingh","year":"1974","journal-title":"Arch. Oral Biol."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0100","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0008-6215(00)85213-1","article-title":"Purification and biochemical characterization of a mouse submandibular sialomucin","volume":"87","author":"Denny","year":"1980","journal-title":"Carbohydr. Res."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0105","doi-asserted-by":"crossref","first-page":"3816","DOI":"10.1021\/ac900157c","article-title":"Supported molecular matrix electrophoresis: a new tool for characterization of glycoproteins","volume":"81","author":"Matsuno","year":"2009","journal-title":"Anal. Chem."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0110","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1002\/elps.201000608","article-title":"Improved method for immunostaining of mucin separated by supported molecular matrix electrophoresis by optimizing the matrix composition and fixation procedure","volume":"32","author":"Matsuno","year":"2011","journal-title":"Electrophoresis"},{"key":"10.1016\/j.bbapap.2018.05.006_bb0115","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.jim.2013.06.002","article-title":"Identification of mucins by using a method involving a combination of on-membrane chemical deglycosylation and immunostaining","volume":"394","author":"Matsuno","year":"2013","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.bbapap.2018.05.006_bb0120","doi-asserted-by":"crossref","first-page":"2432","DOI":"10.1002\/elps.201300154","article-title":"Serum protein fractionation using supported molecular matrix electrophoresis","volume":"34","author":"Dong","year":"2013","journal-title":"Electrophoresis"},{"key":"10.1016\/j.bbapap.2018.05.006_bb0125","doi-asserted-by":"crossref","first-page":"16213","DOI":"10.1021\/ja035660t","article-title":"Elimination of oxidative degradation during the per-O-methylation of carbohydrates","volume":"125","author":"Ciucanu","year":"2003","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0130","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1038\/nmeth1019","article-title":"Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometry","volume":"4","author":"Elias","year":"2007","journal-title":"Nat. Methods"},{"key":"10.1016\/j.bbapap.2018.05.006_bb0135","doi-asserted-by":"crossref","first-page":"7736","DOI":"10.1074\/jbc.M111690200","article-title":"Determination of the site-specific oligosaccharide distribution of the O-glycans attached to the porcine submaxillary mucin tandem repeat. Further evidence for the modulation of O-glycans side chain structures by peptide sequence","volume":"277","author":"Gerken","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0140","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1111\/acel.12337","article-title":"Dysregulation of the Bmi-1\/p16(Ink4a) pathway provokes an aging-associated decline of submandibular gland function","volume":"14","author":"Yamakoshi","year":"2015","journal-title":"Aging Cell"},{"key":"10.1016\/j.bbapap.2018.05.006_bb0145","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1007\/BF03339889","article-title":"Impact of aging on human salivary gland function: a community-based study","volume":"10","author":"Yeh","year":"1998","journal-title":"Aging Clin. Exp. Res."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0150","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1042\/bj1990297","article-title":"A comparative study of the binding of cartilage link protein and the hyaluronate-binding region of the cartilage proteoglycan to hyaluronate-substituted Sepharose gel","volume":"199","author":"Tengblad","year":"1981","journal-title":"Biochem. J."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0155","doi-asserted-by":"crossref","first-page":"4585","DOI":"10.1074\/jbc.R100036200","article-title":"Hyaluronan-binding proteins: tying up the giant","volume":"277","author":"Day","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbapap.2018.05.006_bb0160","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1093\/glycob\/7.4.469","article-title":"Mouse beta-galactoside alpha 2,3-sialyltransferases: comparison of in vitro substrate specificities and tissue specific expression","volume":"7","author":"Kono","year":"1997","journal-title":"Glycobiology"},{"key":"10.1016\/j.bbapap.2018.05.006_bb0165","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1093\/glycob\/6.1.43","article-title":"Mouse submandibular gland salivary apomucin contains repeated N-glycosylation sites","volume":"6","author":"Denny","year":"1996","journal-title":"Glycobiology"}],"container-title":["Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570963918300736?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570963918300736?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,11,1]],"date-time":"2018-11-01T15:34:44Z","timestamp":1541086484000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1570963918300736"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["S1570963918300736"],"URL":"https:\/\/doi.org\/10.1016\/j.bbapap.2018.05.006","relation":{},"ISSN":["1570-9639"],"issn-type":[{"value":"1570-9639","type":"print"}],"subject":[],"published":{"date-parts":[[2019,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A rapid separation and characterization of mucins from mouse submandibular glands by supported molecular matrix electrophoresis","name":"articletitle","label":"Article Title"},{"value":"Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbapap.2018.05.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}