{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T16:06:31Z","timestamp":1787933191088,"version":"build-2784847793"},"reference-count":25,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T00:00:00Z","timestamp":1749686400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018810","name":"University of Southern Denmark VILLUM Center for Bioanalytical Sciences","doi-asserted-by":"publisher","award":["VKR023179"],"award-info":[{"award-number":["VKR023179"]}],"id":[{"id":"10.13039\/501100018810","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018694","name":"HORIZON EUROPE Marie Sklodowska-Curie Actions","doi-asserted-by":"publisher","award":["110361"],"award-info":[{"award-number":["110361"]}],"id":[{"id":"10.13039\/100018694","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018693","name":"Horizon Europe","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018693","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100019180","name":"HORIZON EUROPE European Research Council","doi-asserted-by":"publisher","award":["101088163"],"award-info":[{"award-number":["101088163"]}],"id":[{"id":"10.13039\/100019180","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.bbapap.2025.141084","type":"journal-article","created":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T15:57:18Z","timestamp":1749657438000},"page":"141084","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["Native structure of the monoclonal therapeutic CD20 antibody ocrelizumab"],"prefix":"10.1016","volume":"1873","author":[{"given":"Victor G.","family":"Chrone","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan C.","family":"Jespersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daut C.","family":"Asani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole H.","family":"Trier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soumik","family":"Ray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Berthias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Willemo\u00ebs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Holm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jette L.","family":"Frederiksen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunnar","family":"Houen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"H\u00f8jrup","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbapap.2025.141084_bb0005","series-title":"Janeway's Immunobiology","author":"Murphy","year":"2022"},{"issue":"5517","key":"10.1016\/j.bbapap.2025.141084_bb0010","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/256495a0","article-title":"Continuous cultures of fused cells secreting antibody of predefined specificity","volume":"256","author":"K\u00f6hler","year":"1975","journal-title":"Nature"},{"key":"10.1016\/j.bbapap.2025.141084_bb0015","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/978-3-319-32805-8_2","article-title":"Antibodies and selection of monoclonal antibodies","volume":"917","author":"Hanack","year":"2016","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.bbapap.2025.141084_bb0020","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-0716-1450-1_1","volume":"2313","author":"Houen","year":"2022","journal-title":"Methods Mol. Biol."},{"issue":"1","key":"10.1016\/j.bbapap.2025.141084_bb0025","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1517\/14712598.3.1.141","article-title":"Overview of the use of the anti-TNF agent infliximab in chronic inflammatory diseases","volume":"3","author":"Braun","year":"2003","journal-title":"Expert. Opin. Biol. Ther."},{"issue":"3","key":"10.1016\/j.bbapap.2025.141084_bb0030","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1007\/s40265-022-01672-9","article-title":"Ocrelizumab: A review in multiple sclerosis","volume":"82","author":"Lamb","year":"2022","journal-title":"Drugs"},{"issue":"6","key":"10.1016\/j.bbapap.2025.141084_bb0035","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0217624","article-title":"Immunoglobulin G structure and rheumatoid factor epitopes","volume":"14","author":"Maibom-Thomsen","year":"2019","journal-title":"PLoS ONE"},{"issue":"3","key":"10.1016\/j.bbapap.2025.141084_bb0040","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1159\/000357982","article-title":"Development, manufacturing and characterization of a highly purified, liquid immunoglobulin G preparation from human plasma","volume":"41","author":"Laursen","year":"2014","journal-title":"Transfus. Med. Hemother."},{"issue":"6","key":"10.1016\/j.bbapap.2025.141084_bb0045","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0218374","article-title":"Enhanced trypsin on a budget: stabilization, purification and high-temperature application of inexpensive commercial trypsin for proteomics applications","volume":"14","author":"Heissel","year":"2019","journal-title":"PLoS ONE"},{"key":"10.1016\/j.bbapap.2025.141084_bb0050","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jim.2017.01.006","article-title":"Efficient evaluation of humoral immune responses by the use of serum pools","volume":"443","author":"Sternb\u00e6k","year":"2017","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.bbapap.2025.141084_bb0055","doi-asserted-by":"crossref","first-page":"2856","DOI":"10.1038\/nprot.2006.468","article-title":"In-gel digestion for mass spectrometric characterization of proteins and proteomes","volume":"1","author":"Shevchenko","year":"2006","journal-title":"Nat. Protoc."},{"issue":"12","key":"10.1016\/j.bbapap.2025.141084_bb0060","doi-asserted-by":"crossref","first-page":"2864","DOI":"10.1038\/s41596-018-0068-8","article-title":"A cross-linking\/mass spectrometry workflow based on MS-cleavable cross-linkers and the MeroX software for studying protein structures and protein\u2013protein interactions","volume":"13","author":"Iacobucci","year":"2018","journal-title":"Nat. Protoc."},{"issue":"4","key":"10.1016\/j.bbapap.2025.141084_bb0065","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1002\/mas.20082","article-title":"Chemical cross-linking and mass spectrometry to map three-dimensional protein structures and protein\u2013protein interactions","volume":"25","author":"Sinz","year":"2006","journal-title":"Mass Spectrom. Rev."},{"issue":"1","key":"10.1016\/j.bbapap.2025.141084_bb0070","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s13361-014-1001-1","article-title":"Automated assignment of MS\/MS cleavable cross-links in protein 3D-structure analysis","volume":"26","author":"G\u00f6tze","year":"2015","journal-title":"J. Am. Soc. Mass Spectrom."},{"issue":"17","key":"10.1016\/j.bbapap.2025.141084_bb0075","doi-asserted-by":"crossref","DOI":"10.1016\/j.jmb.2024.168656","article-title":"xiVIEW: visualisation of crosslinking mass spectrometry data","volume":"436","author":"Combe","year":"2024","journal-title":"J. Mol. Biol."},{"issue":"W1","key":"10.1016\/j.bbapap.2025.141084_bb0080","doi-asserted-by":"crossref","first-page":"W296","DOI":"10.1093\/nar\/gky427","article-title":"SWISS-MODEL: homology modelling of protein structures and complexes","volume":"46","author":"Waterhouse","year":"2018","journal-title":"Nucleic Acids Res."},{"issue":"8","key":"10.1016\/j.bbapap.2025.141084_bb0085","doi-asserted-by":"crossref","first-page":"2647","DOI":"10.1093\/bioinformatics\/btaa058","article-title":"QMEANDisCo-distance constraints applied on model quality estimation","volume":"36","author":"Studer","year":"2020","journal-title":"Bioinformatic"},{"issue":"3","key":"10.1016\/j.bbapap.2025.141084_bb0090","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1006\/jmbi.1993.1626","article-title":"Comparative protein modelling by satisfaction of spatial restraints","volume":"234","author":"\u0160ali","year":"1993","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.bbapap.2025.141084_bb0095","author":"Salilab"},{"issue":"1","key":"10.1016\/j.bbapap.2025.141084_bb0100","doi-asserted-by":"crossref","first-page":"7866","DOI":"10.1038\/s41467-024-51771-2","article-title":"Modelling protein complexes with crosslinking mass spectrometry and deep learning","volume":"15","author":"Stahl","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bbapap.2025.141084_bb0105","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.bbapap.2025.141084_bb0110","article-title":"Single-sequence protein structure prediction using a language model and deep learning","author":"Chowdhury","year":"2022","journal-title":"bioRxiv"},{"issue":"9","key":"10.1016\/j.bbapap.2025.141084_bb0115","doi-asserted-by":"crossref","first-page":"2393","DOI":"10.1007\/s00216-017-0185-1","article-title":"Improved single-step enrichment methods of cross-linked products for protein structure analysis and protein interaction mapping","volume":"409","author":"Schmidt","year":"2017","journal-title":"Anal. Bioanal. Chem."},{"issue":"6","key":"10.1016\/j.bbapap.2025.141084_bb0120","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1002\/pro.2458","article-title":"Distance restraints from crosslinking mass spectrometry: mining a molecular dynamics simulation database to evaluate lysine-lysine distances","volume":"23","author":"Merkley","year":"2014","journal-title":"Protein Sci."},{"key":"10.1016\/j.bbapap.2025.141084_bb0125","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1016\/j.csbj.2020.05.007","article-title":"The contorsbody, an antibody format for agonism: design, structure, and function","volume":"18","author":"Georges","year":"2020","journal-title":"Comput. Struct. Biotechnol. J."}],"container-title":["Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570963925000226?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570963925000226?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T07:28:57Z","timestamp":1753860537000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1570963925000226"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":25,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["S1570963925000226"],"URL":"https:\/\/doi.org\/10.1016\/j.bbapap.2025.141084","relation":{},"ISSN":["1570-9639"],"issn-type":[{"value":"1570-9639","type":"print"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Native structure of the monoclonal therapeutic CD20 antibody ocrelizumab","name":"articletitle","label":"Article Title"},{"value":"Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbapap.2025.141084","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"141084"}}