{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T07:24:43Z","timestamp":1778657083946,"version":"3.51.4"},"reference-count":38,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2003,11,1]],"date-time":"2003-11-01T00:00:00Z","timestamp":1067644800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2003,11]]},"DOI":"10.1016\/j.bbrc.2003.10.047","type":"journal-article","created":{"date-parts":[[2003,10,27]],"date-time":"2003-10-27T16:43:29Z","timestamp":1067273009000},"page":"713-720","source":"Crossref","is-referenced-by-count":51,"title":["Crystal structures of BnSP-7 and BnSP-6, two Lys49-phospholipases A2: quaternary structure and inhibition mechanism insights"],"prefix":"10.1016","volume":"311","author":[{"given":"Angelo J.","family":"Magro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreimar M.","family":"Soares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 R.","family":"Giglio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos R.M.","family":"Fontes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2003.10.047_BIB1","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1016\/0041-0101(92)90032-Z","article-title":"Toxic activities of venoms from nine Bothrops species and their correlation with lethality and necrosis","volume":"30","author":"Ferreira","year":"1992","journal-title":"Toxicon"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB2","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1590\/S0104-42301998000400010","article-title":"Obits by venomous snakes in the State of S\u00e3o Paulo: evaluation of 43 cases from 1988 to 1993","volume":"44","author":"Ribeiro","year":"1998","journal-title":"Rev. Ass. Med. Brasil."},{"key":"10.1016\/j.bbrc.2003.10.047_BIB3","series-title":"Handbook of Toxinology","first-page":"67","article-title":"Phospholipases","author":"Rosenberg","year":"1990"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB4","series-title":"Venom Phospholipase A2 Enzymes: Structure, Function and Mechanism","first-page":"1","article-title":"Phospholipase A2: a complex multifunctional protein puzzle","author":"Kini","year":"1997"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB5","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/S0006-2952(02)01210-8","article-title":"Structural and functional characterization of an acidic platelet aggregation inhibitor and hypotensive phospholipase A2 from Bothrops jararacussu snake venom","volume":"64","author":"Andri\u00e3o-Escarso","year":"2002","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.bbrc.2003.10.047_BIB6","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1038\/nsb0695-458","article-title":"Structure-based design of the first potent and selective inhibitor of human non-pancreatic secretory phospholipase A2","volume":"2","author":"Schevitz","year":"1995","journal-title":"Nat. Struct. Biol."},{"key":"10.1016\/j.bbrc.2003.10.047_BIB7","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1016\/0006-3002(63)90792-3","article-title":"The substrate specificity of phospholipase A2","volume":"70","author":"van Deenen","year":"1963","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB8","series-title":"Handbook of Lipid Research","author":"Waite","year":"1987"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S1388-1981(00)00105-0","article-title":"The expanding superfamily of phospholipase A(2) enzymes: classification and characterization","volume":"1488","author":"Six","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB10","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1093\/jb\/118.5.1037","article-title":"Lysine-49-phospholipases A2 from Trimeresurus flavoviridis venom are membrane-acting enzymes","volume":"118","author":"Shimohigashi","year":"1995","journal-title":"J. Biochem."},{"key":"10.1016\/j.bbrc.2003.10.047_BIB11","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/S0041-0101(98)00188-3","article-title":"Lysine 49 phospholipase A2 proteins","volume":"37","author":"Ownby","year":"1999","journal-title":"Toxicon"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB12","doi-asserted-by":"crossref","first-page":"12424","DOI":"10.1021\/bi00164a018","article-title":"Calcium ion independent membrane leakage induced by phospholipase-like myotoxins","volume":"31","author":"Rufini","year":"1992","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB13","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1016\/0041-0101(95)00085-Z","article-title":"Phospholipase A2 myotoxins from Bothrops snake venoms","volume":"33","author":"Guti\u00e9rrez","year":"1995","journal-title":"Toxicon"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB14","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/0003-9861(92)90104-5","article-title":"Isolation and characterization of basic myotoxic phospholipase A2 from Bothrops godmani (Godman\u2019s pit viper) snake venom","volume":"298","author":"D\u0131\u0301az","year":"1992","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.bbrc.2003.10.047_BIB15","doi-asserted-by":"crossref","first-page":"17649","DOI":"10.1016\/S0021-9258(18)38213-9","article-title":"The crystal structure of a lysine 49 phospholipase A2 from the venom of the cottonmouth snake at 2.0\u00c5","volume":"266","author":"Holland","year":"1990","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2003.10.047_BIB16","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1107\/S0907444994011455","article-title":"Structure of a calcium-independent phospholipase-like myotoxic protein from Bothrops asper","volume":"51","author":"Arni","year":"1995","journal-title":"Acta Crystallogr. D"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB17","doi-asserted-by":"crossref","first-page":"329","DOI":"10.2174\/092986650405221017153439","article-title":"Crystal structure of myotoxin-II: a myotoxic phospholipase A2 homologue from Bothrops moojeni venom","volume":"4","author":"de Azevedo","year":"1997","journal-title":"Protein Pept. Lett."},{"key":"10.1016\/j.bbrc.2003.10.047_BIB18","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1002\/(SICI)1097-0134(19980301)30:4<442::AID-PROT11>3.0.CO;2-I","article-title":"Crystallographic and spectroscopic characterization of a molecular hinge: conformational changes in bothropstoxin I, a dimeric Lys49-phospholipase A2 homologue","volume":"30","author":"da Silva-Giotto","year":"1998","journal-title":"Protein Struct. Func. Gen."},{"key":"10.1016\/j.bbrc.2003.10.047_BIB19","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1006\/abbi.1999.1210","article-title":"Crystal structure of myotoxin II, a monomeric Lys49-phospholipase homologue isolated from the venom of Cerrophidion (Bothrops) godmani","volume":"366","author":"Arni","year":"1999","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.bbrc.2003.10.047_BIB20","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/S0041-0101(98)00189-5","article-title":"Structure of a Lys49-phospholipase A2 homologue isolated from the venom of Bothrops nummifer (jumping viper)","volume":"37","author":"deAzevedo","year":"1999","journal-title":"Toxicon"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB21","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1021\/bi0010470","article-title":"Structural basis for low catalytic activity in Lys49-phospholipase A2\u2013A hypothesis: the crystal structure of piratoxin II complexed to fatty acid","volume":"40","author":"Lee","year":"2001","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB22","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S1095-6433(98)10136-8","article-title":"Geographic variations in the composition of myotoxins from Bothrops neuwiedi snake venom: biochemical characterization and biological activity","volume":"121","author":"Rodrigues","year":"1998","journal-title":"Comp. Biochem. Physiol. A"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB23","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1006\/abbi.2000.1790","article-title":"Structural and functional characterization of BnSP-7, a Lys49 myotoxic phospholipas A2 homologue from Bothrops neuwiedi pauloensis venom","volume":"378","author":"Soares","year":"2000","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.bbrc.2003.10.047_BIB24","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1006\/abbi.1999.1492","article-title":"Structural and functional characterization of myotoxin I, a Lys49 phospholipase A2 from Bothrops moojeni (caissaca) snake venom","volume":"373","author":"Soares","year":"2000","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.bbrc.2003.10.047_BIB25","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/S0167-4838(99)00120-X","article-title":"Crystallization and preliminary X-ray diffraction analysis of a myotoxic phospholipase A2 homologue from Bothrops neuwiedi pauloensis venom","volume":"1432","author":"Fontes","year":"1999","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB26","doi-asserted-by":"crossref","first-page":"421","DOI":"10.2174\/092986650706221208100629","article-title":"Crystallization and preliminary X-ray diffraction studies of BnSP-6, a myotoxic phospholipase A2 from Bothrops neuwiedi venom","volume":"7","author":"Fontes","year":"2000","journal-title":"Protein Pept. Lett."},{"key":"10.1016\/j.bbrc.2003.10.047_BIB27","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0076-6879(97)76066-X","article-title":"Processing of X-ray diffraction data collected in oscillation mode","volume":"276","author":"Otwinowski","year":"1997","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.bbrc.2003.10.047_BIB28","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1107\/S0108767393007597","article-title":"AMoRe: an automated package for molecular replacement","volume":"50","author":"Navaza","year":"1994","journal-title":"Acta. Crystallogr. A"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB29","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1107\/S0907444998003254","article-title":"Crystallography and NMR system (CNS): a new software system for macromolecular structure determination","volume":"54","author":"Br\u00fc nger","year":"1998","journal-title":"Acta Crystallogr. D"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB30","series-title":"Crystallographic and Modeling Methods in Molecular Design","first-page":"189","article-title":"O: a macromolecule modeling environment","author":"Jones","year":"1990"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB31","series-title":"X-PLOR Version 3.1: A System for Crystallography and NMR","author":"Br\u00fc nger","year":"1992"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB32","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1107\/S0021889892009944","article-title":"Procheck: a program to check the stereochemical quality of protein structures","volume":"26","author":"Laskowski","year":"1993","journal-title":"J. Appl. Crystallogr."},{"key":"10.1016\/j.bbrc.2003.10.047_BIB33","doi-asserted-by":"crossref","unstructured":"Collaborative Computing Project No. 4 (CCP4), The CCP4 suite: programs for protein crystallography, Acta Crystallogr. D 50 (1994) 760\u2013763","DOI":"10.1107\/S0907444994003112"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB34","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/S0076-6879(97)77027-7","article-title":"Ribbons","volume":"277","author":"Carson","year":"1997","journal-title":"Methods. Enzymol."},{"key":"10.1016\/j.bbrc.2003.10.047_BIB35","doi-asserted-by":"crossref","first-page":"11627","DOI":"10.1016\/S0021-9258(17)39077-4","article-title":"A comparison of the crystal structures of phospholipases A2 from bovine pancreas and Crotalus atrox venom","volume":"260","author":"Renetseder","year":"1985","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2003.10.047_BIB36","unstructured":"M.R.M. Fontes, L. Watanabe, A.M. Soares, R.K. Arni, Structural insights to fatty acid binding to Lys49 phospholipase A2s: crystal structure of myotoxin II from Bothrops moojeni complexed with stearic acid at 1.6\u00c5 resolution, FEBS Lett. (2003) (submitted)"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB37","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1016\/S0041-0101(98)00155-X","article-title":"At the interface: crystal structures of phospholipases A2","volume":"36","author":"Ward","year":"1998","journal-title":"Toxicon"},{"key":"10.1016\/j.bbrc.2003.10.047_BIB38","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1016\/S0041-0101(98)00017-8","article-title":"Crystal structure of piratoxin-I: a calcium-independent, myotoxic phospholipase A2 homologue from Bothrops pirajai venom","volume":"36","author":"de Azevedo","year":"1998","journal-title":"Toxicon"}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X03021302?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X03021302?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T08:03:41Z","timestamp":1682582621000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X03021302"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,11]]},"references-count":38,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2003,11]]}},"alternative-id":["S0006291X03021302"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2003.10.047","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2003,11]]}}}