{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T00:02:56Z","timestamp":1790985776739,"version":"4.1.0"},"reference-count":43,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2004,1]]},"DOI":"10.1016\/j.bbrc.2003.12.038","type":"journal-article","created":{"date-parts":[[2004,1,12]],"date-time":"2004-01-12T08:55:03Z","timestamp":1073897703000},"page":"1044-1052","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":52,"title":["Direct evidence for cyanide-insensitive quinol oxidase (alternative oxidase) in apicomplexan parasite Cryptosporidium parvum: phylogenetic and therapeutic implications"],"prefix":"10.1016","volume":"313","author":[{"given":"Takashi","family":"Suzuki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuo","family":"Hashimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshisada","family":"Yabu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasutoshi","family":"Kido","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimitoshi","family":"Sakamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coh-ichi","family":"Nihei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariko","family":"Hato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-ichi","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Amano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuo","family":"Nagai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoyoshi","family":"Hosokawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuko","family":"Minagawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuo","family":"Ohta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyoshi","family":"Kita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2003.12.038_BIB1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/S0005-2728(01)00237-7","article-title":"Role of complex II in anaerobic respiration of the parasite mitochondria from Ascaris suum and Plasmodium falciparum","volume":"1553","author":"Kita","year":"2002","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbrc.2003.12.038_BIB2","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/S0925-4439(02)00086-8","article-title":"Trypanosome alternative oxidase as a target of chemotherapy","volume":"1587","author":"Nihei","year":"2002","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbrc.2003.12.038_BIB3","doi-asserted-by":"crossref","first-page":"S57","DOI":"10.1093\/clinids\/21.Supplement_1.S57","article-title":"Cryptosporidiosis: sources of infection and guidelines for prevention","volume":"21","author":"Juranek","year":"1995","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB4","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1056\/NEJM198305263082102","article-title":"Human cryptosporidiosis in immunocompetent and immunodeficient persons. Studies of an outbreak and experimental transmission","volume":"308","author":"Current","year":"1983","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB5","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1128\/iai.64.1.349-354.1996","article-title":"Complete development of Cryptosporidium parvum in bovine fallopian tube epithelial cells","volume":"64","author":"Yang","year":"1996","journal-title":"Infect. Immun."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB6","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1128\/iai.54.1.255-259.1986","article-title":"Experimental intrauterine infection of adult BALB\/c mice with Cryptosporidium sp","volume":"54","author":"Liebler","year":"1986","journal-title":"Infect. Immun."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB7","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/S0169-4758(99)01474-X","article-title":"Biochmical peculiarities and drug targets in Cryptosporidium parvum: lessons from other coccidian parasites","volume":"15","author":"Coombs","year":"1999","journal-title":"Parasitol. Today"},{"key":"10.1016\/j.bbrc.2003.12.038_BIB8","first-page":"S52","article-title":"Preliminary evidence for a Mitochondrion in Cryptosporidium parvum: phylogenetic and therapeutic implications","volume":"46","author":"Riordan","year":"1999","journal-title":"J. Eukaryot. Microbiol."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB9","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1007\/s002940000183","article-title":"Characterization of two genes encoding the mitochondrial alternative oxidase in Chlamydomonas reinhardtii","volume":"39","author":"Dinant","year":"2001","journal-title":"Curr. Genet."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB10","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1146\/annurev.pp.26.060175.001001","article-title":"Thermogenic respiration in aroids","volume":"26","author":"Meeuse","year":"1975","journal-title":"Annu. Rev. Plant Physiol."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB11","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1104\/pp.105.3.781","article-title":"Molecular biology of the alternative oxidase","volume":"105","author":"MacIntosh","year":"1994","journal-title":"Plant Physiol."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB12","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1093\/oxfordjournals.jbchem.a121978","article-title":"The induction of cyanide-resistant respiration in Hansenula anomala","volume":"101","author":"Minagawa","year":"1987","journal-title":"J. Biochem."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB13","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1006\/dbio.1995.0014","article-title":"Involvement of cyanide-resistant respiration in cell-type proportioning during Dictyostelium development","volume":"172","author":"Matsuyama","year":"1995","journal-title":"Dev. Biol."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB14","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S0014-5793(01)03261-6","article-title":"Exploring the molecular nature of alternative oxidase regulation and catalysis","volume":"510","author":"Affourtit","year":"2002","journal-title":"FEBS Lett."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB15","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/0014-5793(90)80302-Y","article-title":"Essential role of ferrous iron in cyanide-resistant respiration in Hansenula anomala","volume":"267","author":"Minagawa","year":"1990","journal-title":"FEBS Lett."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB16","doi-asserted-by":"crossref","first-page":"8187","DOI":"10.1074\/jbc.M111477200","article-title":"Site-directed mutagenesis reveals the essentiality of the conserved residues in the putative diiron active site of the trypanosome alternative oxidase","volume":"277","author":"Ajayi","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB17","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/0014-5793(95)00196-G","article-title":"The active site of the cyanide-resistant oxidase from plant mitochondria contains a binuclear iron center","volume":"362","author":"Siedow","year":"1995","journal-title":"FEBS Lett."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB18","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0014-5793(99)00376-2","article-title":"A revised model of the active site of alternative oxidase","volume":"449","author":"Andersson","year":"1999","journal-title":"FEBS Lett."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB19","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0014-5793(00)01943-8","article-title":"New sequence data enable modelling of the fungal alternative oxidase and explain an absence of regulation by pyruvate","volume":"481","author":"Joseph-Horne","year":"2000","journal-title":"FEBS Lett."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB20","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/S0166-6851(96)02797-1","article-title":"An antibiotic, ascofuranone, specifically inhibits respiration and in vitro growth of long slender bloodstream forms of Trypanosoma brucei brucei","volume":"84","author":"Minagawa","year":"1997","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB21","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/S1383-5769(98)00011-7","article-title":"Oral and intraperitoneal treatment of Trypanosoma brucei brucei with a combination of ascofuranone and glycerol in mice","volume":"47","author":"Yabu","year":"1998","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB22","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S1383-5769(03)00007-2","article-title":"Overproduction of highly active trypanosome alternative oxidase in Escherichia coli heme-deficient mutant","volume":"52","author":"Fukai","year":"2003","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB23","unstructured":"J. Adachi, M. Hasegawa, MOLPHY Version 2.3: Programs for Molecular Phylogenetics Based on Maximum Likelihood, Computer Science Monographs, The Institute of Statistical Mathematics, 28, 1996"},{"key":"10.1016\/j.bbrc.2003.12.038_BIB24","first-page":"555","article-title":"PAML: a program package for phylogenetic analysis by maximum likelihood","volume":"13","author":"Yang","year":"1997","journal-title":"Comput. Appl. Biosci."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB25","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications","volume":"76","author":"Towbin","year":"1979","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bbrc.2003.12.038_BIB26","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB27","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1146\/annurev.arplant.54.031902.134915","article-title":"Membrane-bound diiron carboxylate proteins","volume":"54","author":"Berthold","year":"2003","journal-title":"Annu. Rev. Plant Biol."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB28","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0014-5793(03)00120-0","article-title":"Purification of active trypanosome alternative oxidase","volume":"538","author":"Nihei","year":"2003","journal-title":"FEBS Lett."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB29","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1006\/expr.1997.4194","article-title":"Plasmodium falciparum: cyanide-resistant oxygen consumption","volume":"87","author":"Murphy","year":"1997","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB30","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1038\/nature01097","article-title":"Genome sequence of the human malaria parasite Plasmodium falciparum","volume":"419","author":"Gardner","year":"2002","journal-title":"Nature"},{"issue":"Pt. 4","key":"10.1016\/j.bbrc.2003.12.038_BIB31","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1099\/00207713-50-4-1673","article-title":"What is the phylogenetic position of Cryptosporidium?","volume":"50","author":"Zhu","year":"2000","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB32","doi-asserted-by":"crossref","first-page":"7678","DOI":"10.1073\/pnas.1230951100","article-title":"Lateral gene transfer and the evolution of plastid-targeted proteins in the secondary plastid-containing alga Bigelowiella natans","volume":"100","author":"Archibald","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bbrc.2003.12.038_BIB33","first-page":"141","article-title":"Functional expression of the ascofuranone-sensitive Trypanosoma brucei brucei alternative oxidase in the cytoplasmic membrane of Escherichia coli","volume":"124","author":"Fukai","year":"1999","journal-title":"Comp. Biochem. Physiol. C"},{"key":"10.1016\/j.bbrc.2003.12.038_BIB34","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1093\/bioinformatics\/18.2.298","article-title":"Extensive feature detection of N-terminal protein sorting signals","volume":"18","author":"Bannai","year":"2002","journal-title":"Bioinformatics"},{"key":"10.1016\/j.bbrc.2003.12.038_BIB35","doi-asserted-by":"crossref","first-page":"3249","DOI":"10.1099\/00221287-144-12-3249","article-title":"Ultrastructural analysis of the sporozoite of Cryptosporidium parvum","volume":"144","author":"Tetley","year":"1998","journal-title":"Microbiology"},{"key":"10.1016\/j.bbrc.2003.12.038_BIB36","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1111\/j.1574-6968.1996.tb08431.x","article-title":"The use of a new viability assay to determine the susceptibility of Cryptosporidium and Eimeria sporozoites to respiratory inhibitors and extremes of pH","volume":"142","author":"Brown","year":"1996","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB37","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/0166-6851(95)02527-8","article-title":"Comparison of the phosphofructokinase and pyruvate kinase activities of Cryptosporidium parvum, Eimeria tenella and Toxoplasma gondii","volume":"76","author":"Denton","year":"1996","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB38","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1111\/j.1574-6968.1997.tb10393.x","article-title":"Glycolytic enzyme activities in Cryptosporidium parvum oocysts","volume":"151","author":"Entrala","year":"1997","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB39","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1146\/annurev.pp.26.060175.001001","article-title":"Thermogenic respiration in aroids","volume":"26","author":"Meeuse","year":"1975","journal-title":"Annu. Rev. Plant Physiol."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB40","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1111\/j.1550-7408.1995.tb05892.x","article-title":"Identification and partial purification of a stage-specific 33kDa mitochondrial protein as the alternative oxidase of the Trypanosoma brucei brucei bloodstream trypomastigotes","volume":"42","author":"Chaudhuri","year":"1995","journal-title":"J. Eukaryot. Microbiol."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB41","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0166-6851(96)02754-5","article-title":"Cloning, sequencing, and functional activity of the Trypanosoma brucei brucei alternative oxidase","volume":"83","author":"Chaudhuri","year":"1996","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.bbrc.2003.12.038_BIB42","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.7164\/antibiotics.35.1547","article-title":"Antitumor protective property of an isoprenoid antibiotic, ascofuranone","volume":"35","author":"Magae","year":"1982","journal-title":"J. Antibiot. (Tokyo)"},{"key":"10.1016\/j.bbrc.2003.12.038_BIB43","doi-asserted-by":"crossref","first-page":"892","DOI":"10.7164\/antibiotics.36.892","article-title":"Effects of an antitumor agent, ascofuranone, on the macromolecular syntheses of intact cells","volume":"36","author":"Magae","year":"1983","journal-title":"J. Antibiot. (Tokyo)"}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X03026214?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X03026214?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T14:09:07Z","timestamp":1762092547000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X03026214"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1]]},"references-count":43,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["S0006291X03026214"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2003.12.038","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2004,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Direct evidence for cyanide-insensitive quinol oxidase (alternative oxidase) in apicomplexan parasite Cryptosporidium parvum: phylogenetic and therapeutic implications","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2003.12.038","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"converted-article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2003 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}