{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,4,28]],"date-time":"2023-04-28T05:10:14Z","timestamp":1682658614450},"reference-count":42,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2004,4,1]],"date-time":"2004-04-01T00:00:00Z","timestamp":1080777600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2004,4]]},"DOI":"10.1016\/j.bbrc.2004.03.044","type":"journal-article","created":{"date-parts":[[2004,3,20]],"date-time":"2004-03-20T16:11:47Z","timestamp":1079799107000},"page":"294-300","source":"Crossref","is-referenced-by-count":1,"title":["Western blot analysis of the exotoxin components from Bacillus anthracis separated by isoelectric focusing gel electrophoresis"],"prefix":"10.1016","volume":"317","author":[{"given":"Stephen F","family":"Little","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2004.03.044_BIB1","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1128\/IAI.49.2.291-297.1985","article-title":"Demonstration of a capsule plasmid in Bacillus anthracis","volume":"49","author":"Green","year":"1985","journal-title":"Infect. Immun."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB2","series-title":"Bacterial Toxins and Virulence Factors in Disease","first-page":"543","article-title":"Anthrax toxins","author":"Leppla","year":"1995"},{"key":"10.1016\/j.bbrc.2004.03.044_BIB3","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1128\/IAI.39.1.371-376.1983","article-title":"Evidence for plasmid-mediated toxin production in Bacillus anthracis","volume":"39","author":"Mikesell","year":"1983","journal-title":"Infect. Immun."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB4","doi-asserted-by":"crossref","first-page":"7123","DOI":"10.1016\/S0021-9258(17)38364-3","article-title":"Macrophages are sensitive to anthrax lethal toxin through an acid-dependent process","volume":"261","author":"Friedlander","year":"1986","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB5","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1126\/science.280.5364.734","article-title":"Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor","volume":"280","author":"Duesbery","year":"1998","journal-title":"Science"},{"key":"10.1016\/j.bbrc.2004.03.044_BIB6","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1006\/bbrc.1998.9040","article-title":"Anthrax lethal factor cleaves the N-terminus of MAPKKs and induces tyrosine\/threonine phosphorylation of MAPKs in cultured macrophages","volume":"248","author":"Vitale","year":"1998","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB7","doi-asserted-by":"crossref","first-page":"3162","DOI":"10.1073\/pnas.79.10.3162","article-title":"Anthrax toxin edema factor: a bacterial adenylate cyclase that increases cyclic AMP concentrations of eukaryotic cells","volume":"79","author":"Leppla","year":"1982","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bbrc.2004.03.044_BIB8","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1038\/n35101999","article-title":"Identification of the cellular receptor for anthrax toxin","volume":"414","author":"Bradley","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.bbrc.2004.03.044_BIB9","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1099\/00221287-138-3-543","article-title":"Serum protease cleavage of Bacillus anthracis protective antigen","volume":"138","author":"Ezzell","year":"1992","journal-title":"J. Gen. Microbiol."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB10","doi-asserted-by":"crossref","first-page":"10277","DOI":"10.1073\/pnas.89.21.10277","article-title":"Anthrax toxin protective antigen is activated by a cell surface protease with the sequence specificity and catalytic properties of furin","volume":"89","author":"Klimpel","year":"1992","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bbrc.2004.03.044_BIB11","unstructured":"S.H. Leppla, A.M. Friedlander, E.M. Cora, Proteolytic activation of anthrax toxin bound to cellular receptors, in: F.J. Fehrenbach, J.E. Alour, P. Falmagne, W. Goebel, J. Jeljaszewicz, D. Jurgen, R. Rappuoli (Eds.), Bacterial Protein Toxins, Zbl. Bakt. Suppl. 17, Gustav Fischer, New York, 1988, pp. 111\u2013112"},{"key":"10.1016\/j.bbrc.2004.03.044_BIB12","doi-asserted-by":"crossref","first-page":"20607","DOI":"10.1016\/S0021-9258(17)32036-7","article-title":"Anthrax protective antigen forms oligomers during intoxication of mammalian cells","volume":"269","author":"Milne","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB13","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1046\/j.1462-5822.2000.00052.x","article-title":"Proteolytic activation of receptor-bound anthrax protective antigen on macrophages promotes its internalization","volume":"2","author":"Beauregard","year":"2000","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB14","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1128\/IAI.56.5.1066-1069.1988","article-title":"Inhibitors of receptor-mediated endocytosis block the entry of Bacillus anthracis adenylate cyclase toxin but not that of Bordetella pertussis adenylate cyclase toxin","volume":"56","author":"Gordon","year":"1988","journal-title":"Infect. Immun."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB15","doi-asserted-by":"crossref","first-page":"3941","DOI":"10.1021\/bi972657b","article-title":"Identification of residues lining the anthrax protective antigen channel","volume":"37","author":"Benson","year":"1998","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbrc.2004.03.044_BIB16","doi-asserted-by":"crossref","first-page":"10432","DOI":"10.1021\/bi990792d","article-title":"Anthrax protective antigen: prepore-to-pore conversion","volume":"38","author":"Miller","year":"1999","journal-title":"Biochem."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB17","first-page":"307","article-title":"The use of anthrax vaccines prepared from avirulent (unencapsulated) variants of Bacillus anthracis","volume":"13","author":"Sterne","year":"1939","journal-title":"Onderstepoort J. Vet. Sci. Anim. Indus."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB18","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/0264-410X(94)90223-2","article-title":"Human live anthrax vaccine in the former USSR","volume":"12","author":"Shlyakhov","year":"1994","journal-title":"Vaccine"},{"key":"10.1016\/j.bbrc.2004.03.044_BIB19","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/0168-1656(95)00092-5","article-title":"Development of novel vaccines against anthrax in man","volume":"44","author":"Stepanov","year":"1996","journal-title":"J. Biotech."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB20","doi-asserted-by":"crossref","first-page":"129","DOI":"10.4049\/jimmunol.75.2.129","article-title":"Studies on immunity in anthrax VI. Immunizing activity of protective antigen against various strains of Bacillus anthracis","volume":"75","author":"Auerbach","year":"1955","journal-title":"J. Immunol."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB21","doi-asserted-by":"crossref","first-page":"3241","DOI":"10.1016\/S0264-410X(01)00021-4","article-title":"Efficacy of a human anthrax vaccine in guinea pigs, rabbits, and rhesus macaques against challenge by Bacillus anthracis isolates of diverse geographical origin","volume":"19","author":"Fellows","year":"2001","journal-title":"Vaccine"},{"key":"10.1016\/j.bbrc.2004.03.044_BIB22","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1128\/IAI.52.2.509-512.1986","article-title":"Comparative efficacy of Bacillus anthracis live spore vaccine and protective antigen vaccine against anthrax in the guinea pig","volume":"52","author":"Little","year":"1986","journal-title":"Infect. Immun."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB23","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1128\/IAI.52.2.356-363.1986","article-title":"Development of antibodies to protective antigen and lethal factor components of anthrax toxin in humans and guinea pigs and their relevance to protective immunity","volume":"52","author":"Turnbull","year":"1986","journal-title":"Infect. Immun."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB24","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1093\/infdis\/115.1.59","article-title":"Studies on anthrax infections in immunized guinea pigs","volume":"115","author":"Ward","year":"1965","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB25","doi-asserted-by":"crossref","first-page":"2928","DOI":"10.1128\/JB.182.10.2928-2936.2000","article-title":"Multiple-locus variable-number tandem repeat analysis reveals genetic relationships within Bacillus anthracis","volume":"182","author":"Keim","year":"2000","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB26","doi-asserted-by":"crossref","first-page":"2358","DOI":"10.1128\/JB.181.8.2358-2362.1999","article-title":"Genetic diversity in the protective antigen gene of Bacillus anthracis","volume":"181","author":"Price","year":"1999","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB27","first-page":"103","article-title":"Production and purification of anthrax toxin","volume":"vol. 165","author":"Leppla","year":"1988"},{"key":"10.1016\/j.bbrc.2004.03.044_BIB28","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/0003-2697(84)90486-X","article-title":"Analytical isoelectric focusing using a high-voltage vertical slab polyacrylamide gel system","volume":"142","author":"Giulian","year":"1984","journal-title":"Anal Biochem"},{"key":"10.1016\/j.bbrc.2004.03.044_BIB29","doi-asserted-by":"crossref","first-page":"1807","DOI":"10.1128\/IAI.56.7.1807-1813.1988","article-title":"Production and characterization of monoclonal antibodies to the protective antigen component of Bacillus anthracis toxin","volume":"56","author":"Little","year":"1988","journal-title":"Infect. Immun."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB30","doi-asserted-by":"crossref","first-page":"1606","DOI":"10.1128\/IAI.58.6.1606-1613.1990","article-title":"Production and characterization of monoclonal antibodies against the lethal factor component of Bacillus anthracis lethal toxin","volume":"58","author":"Little","year":"1990","journal-title":"Infect. Immun."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB31","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1006\/bbrc.1994.1281","article-title":"Structure\u2013function analysis of Bacillus anthracis edema factor by using monoclonal antibodies","volume":"199","author":"Little","year":"1994","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB32","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/0022-1759(89)90397-9","article-title":"Re-examination and further development of a precise and rapid dye method for measuring cell growth\/cell kill","volume":"119","author":"Hansen","year":"1989","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.bbrc.2004.03.044_BIB33","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1128\/IAI.39.1.483-486.1983","article-title":"Elaboration of Bacillus anthracis antigens in a new, defined culture medium","volume":"39","author":"Ristroph","year":"1983","journal-title":"Infect. Immun."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB34","series-title":"Sourcebook of Bacterial Protein Toxins","first-page":"277","article-title":"The anthrax toxin complex","author":"Leppla","year":"1991"},{"key":"10.1016\/j.bbrc.2004.03.044_BIB35","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1042\/bj0630437","article-title":"Polyglutamic acid from Bacillus anthracis grown in vivo: structure and aggressin activity","volume":"63","author":"Zwartouw","year":"1956","journal-title":"Biochem. J."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB36","doi-asserted-by":"crossref","first-page":"4768","DOI":"10.1016\/S0264-410X(01)00234-1","article-title":"In vitro correlate of immunity in a rabbit model of inhalational anthrax","volume":"19","author":"Pitt","year":"2001","journal-title":"Vaccine"},{"key":"10.1016\/j.bbrc.2004.03.044_BIB37","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1128\/IAI.52.2.454-458.1986","article-title":"Immunization studies with attenuated strains of Bacillus anthracis","volume":"52","author":"Ivins","year":"1986","journal-title":"Infect. Immun."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB38","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1128\/IAI.60.2.662-668.1992","article-title":"Immunization against anthrax with Bacillus anthracis protective antigen combined with adjuvants","volume":"60","author":"Ivins","year":"1992","journal-title":"Infect. Immun."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB39","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1007\/BF00189414","article-title":"Antibodies to anthrax toxin in humans and guinea pigs and their relevance to protective immunity","volume":"177","author":"Turnbull","year":"1988","journal-title":"Med. Microbiol. Immunol."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB40","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1128\/JB.83.3.515-522.1962","article-title":"Studies on immunity in anthrax IX. Effect of variations in cultural conditions of elaboration of protective antigen by strains of Bacillus anthracis","volume":"83","author":"Wright","year":"1961","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB41","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1128\/JB.72.5.666-672.1956","article-title":"Survival time as a rapid method of determining virulence with Bacillus anthracis","volume":"72","author":"Roth","year":"1956","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.bbrc.2004.03.044_BIB42","first-page":"6","article-title":"An epidemic of inhalation anthrax: the first in the twentieth century","volume":"72","author":"Brachman","year":"1960","journal-title":"Am. J. Hyg."}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X04005376?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X04005376?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,4,28]],"date-time":"2023-04-28T04:40:55Z","timestamp":1682656855000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X04005376"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,4]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,4]]}},"alternative-id":["S0006291X04005376"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2004.03.044","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2004,4]]}}}