{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:09:37Z","timestamp":1791925777614,"version":"4.3.4"},"reference-count":21,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2004,6,1]],"date-time":"2004-06-01T00:00:00Z","timestamp":1086048000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2004,6]]},"DOI":"10.1016\/j.bbrc.2004.05.017","type":"journal-article","created":{"date-parts":[[2004,5,29]],"date-time":"2004-05-29T05:09:56Z","timestamp":1085807396000},"page":"486-492","source":"Crossref","is-referenced-by-count":22,"title":["Modifying the substrate specificity of penicillin G acylase to cephalosporin acylase by mutating active-site residues"],"prefix":"10.1016","volume":"319","author":[{"given":"Bora","family":"Oh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyunggon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jungeun","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jongchul","family":"Yoon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dohyun","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youngsoo","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2004.05.017_BIB1","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/S0959-440X(96)80059-5","article-title":"Structural classification of proteins: new superfamilies","volume":"6","author":"Murzin","year":"1996","journal-title":"Curr. Opin. Struct. Biol."},{"key":"10.1016\/j.bbrc.2004.05.017_BIB2","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1016\/S0022-2836(05)80134-2","article-title":"SCOP: a structural classification of proteins database for the investigation of sequences and structures","volume":"247","author":"Murzin","year":"1995","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.bbrc.2004.05.017_BIB3","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1016\/S0969-2126(00)00505-0","article-title":"The 2.0\u00c5 crystal structure of cephalosporin acylase","volume":"8","author":"Kim","year":"2000","journal-title":"Structure"},{"key":"10.1016\/j.bbrc.2004.05.017_BIB4","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1016\/S1074-5521(01)00092-8","article-title":"Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity","volume":"8","author":"Kim","year":"2001","journal-title":"Chem. Biol."},{"key":"10.1016\/j.bbrc.2004.05.017_BIB5","series-title":"Chemistry and Biology","first-page":"27","article-title":"Preparative methods for 7-aminocephalosporanic acid and 6-aminopenicillanic acid","author":"Huber","year":"1972"},{"key":"10.1016\/j.bbrc.2004.05.017_BIB6","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.bbrc.2003.08.110","article-title":"Deacylation activity of cephalosporin acylase to cephalosporin C is improved by changing the side-chain conformations of active-site residues","volume":"310","author":"Oh","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2004.05.017_BIB7","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1038\/373264a0","article-title":"Penicillin acylase has a single-amino-acid catalytic centre","volume":"373","author":"Duggleby","year":"1995","journal-title":"Nature"},{"key":"10.1016\/j.bbrc.2004.05.017_BIB8","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1006\/jmbi.1998.2180","article-title":"Ligand-induced conformational change in penicillin acylase","volume":"284","author":"Done","year":"1998","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.bbrc.2004.05.017_BIB9","first-page":"200","article-title":"Isolation of novel Pseudomonas diminuta KAC-1 strain producing glutaryl 7-aminocephalosporanic acid acylase","volume":"37","author":"Kim","year":"1999","journal-title":"J. Microbiol."},{"key":"10.1016\/j.bbrc.2004.05.017_BIB10","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1046\/j.1432-1327.1999.00417.x","article-title":"In vivo post-translational processing and subunit reconstitution of cephalosporin acylase from Pseudomonas sp. 130","volume":"262","author":"Li","year":"1999","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.bbrc.2004.05.017_BIB11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/AEM.11.1.1-6.1963","article-title":"Distribution and substrate specificity of benzylpenicillin acylase","volume":"11","author":"Huang","year":"1963","journal-title":"Appl. Microbiol."},{"key":"10.1016\/j.bbrc.2004.05.017_BIB12","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1006\/bbrc.2000.2729","article-title":"Identification of a new active site for autocatalytic processing of penicillin acylase precursor in Escherichia coli ATCC11105","volume":"272","author":"Lee","year":"2000","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2004.05.017_BIB13","series-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"10.1016\/j.bbrc.2004.05.017_BIB14","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1042\/bj1150733","article-title":"Hydrolysis of penicillins and related compounds by the cell-bound penicillin assay of Escherichia coli","volume":"115","author":"Cole","year":"1969","journal-title":"Biochem. J."},{"key":"10.1016\/j.bbrc.2004.05.017_BIB15","first-page":"375","article-title":"Cloning and characterization of GL-7-ACA acylase gene from Pseudomonas sp. GK16","volume":"6","author":"Lee","year":"1996","journal-title":"J. Microbiol. Biotechnol."},{"key":"10.1016\/j.bbrc.2004.05.017_BIB16","doi-asserted-by":"crossref","first-page":"4576","DOI":"10.1128\/JB.180.17.4576-4582.1998","article-title":"Two-step autocatalytic processing of the glutaryl 7-aminocephalosporanic acid acylase from Pseudomonas sp. strainGK16","volume":"180","author":"Lee","year":"1998","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.bbrc.2004.05.017_BIB17","doi-asserted-by":"crossref","first-page":"48376","DOI":"10.1074\/jbc.M109603200","article-title":"Active site residues of cephalosporin acylase are critical not only for enzymatic catalysis but also for post-translational modification","volume":"276","author":"Kim","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2004.05.017_BIB18","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1074\/jbc.M309281200","article-title":"A bound water molecule is crucial in initiating autocatalytic precursor activation in an N-terminal hydrolase","volume":"279","author":"Yoon","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2004.05.017_BIB19","doi-asserted-by":"crossref","first-page":"2823","DOI":"10.1074\/jbc.M108888200","article-title":"Precursor structure of cephalosporin acylase: insights into autoproteolytic activation in a new N-terminal hydrolase family","volume":"277","author":"Kim","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2004.05.017_BIB20","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1107\/S0108767390010224","article-title":"Improved methods for binding protein models in electron density maps and the location of errors in these models","volume":"47","author":"Jones","year":"1991","journal-title":"Acta Crystallogr. A"},{"key":"10.1016\/j.bbrc.2004.05.017_BIB21","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1111\/j.1749-6632.1992.tb35664.x","article-title":"Biocatalytic 7-aminocephalosporanic acid production","volume":"672","author":"Tischer","year":"1992","journal-title":"Ann. N. Y. Acad. Sci."}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X04009623?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X04009623?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,4,2]],"date-time":"2020-04-02T08:48:57Z","timestamp":1585817337000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X04009623"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,6]]},"references-count":21,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,6]]}},"alternative-id":["S0006291X04009623"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2004.05.017","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2004,6]]}}}