{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T20:08:20Z","timestamp":1779307700188,"version":"3.51.4"},"reference-count":19,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2014,2,1]],"date-time":"2014-02-01T00:00:00Z","timestamp":1391212800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2014,2,1]],"date-time":"2014-02-01T00:00:00Z","timestamp":1391212800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100007434","name":"Suzuken Memorial Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007434","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000901","name":"Juvenile Diabetes Research Foundation International","doi-asserted-by":"publisher","award":["10-2010-561"],"award-info":[{"award-number":["10-2010-561"]}],"id":[{"id":"10.13039\/100000901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000901","name":"Juvenile Diabetes Research Foundation International","doi-asserted-by":"publisher","award":["25461348"],"award-info":[{"award-number":["25461348"]}],"id":[{"id":"10.13039\/100000901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2014,2]]},"DOI":"10.1016\/j.bbrc.2014.01.083","type":"journal-article","created":{"date-parts":[[2014,1,25]],"date-time":"2014-01-25T06:58:26Z","timestamp":1390633106000},"page":"514-519","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"title":["Sequential introduction and dosage balance of defined transcription factors affect reprogramming efficiency from pancreatic duct cells into insulin-producing cells"],"prefix":"10.1016","volume":"444","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7499-9919","authenticated-orcid":false,"given":"Kazuyuki","family":"Miyashita","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Miyatsuka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taka-Aki","family":"Matsuoka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shugo","family":"Sasaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satomi","family":"Takebe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuyuki","family":"Yasuda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirotaka","family":"Watada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideaki","family":"Kaneto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iichiro","family":"Shimomura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2014.01.083_b0005","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1038\/nbt1259","article-title":"Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells","volume":"24","author":"D\u2019Amour","year":"2006","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.bbrc.2014.01.083_b0010","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1038\/nature07314","article-title":"In vivo reprogramming of adult pancreatic exocrine cells to beta-cells","volume":"455","author":"Zhou","year":"2008","journal-title":"Nature"},{"key":"10.1016\/j.bbrc.2014.01.083_b0015","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1038\/nature08894","article-title":"Conversion of adult pancreatic alpha-cells to beta-cells after extreme beta-cell loss","volume":"464","author":"Thorel","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.bbrc.2014.01.083_b0020","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1038\/nbt.2747","article-title":"Transient cytokine treatment induces acinar cell reprogramming and regenerates functional beta cell mass in diabetic mice","volume":"32","author":"Baeyens","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.bbrc.2014.01.083_b0025","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1517\/14728222.12.11.1431","article-title":"Transcription factors as therapeutic targets for diabetes","volume":"12","author":"Miyatsuka","year":"2008","journal-title":"Expert Opin. Ther. Targets"},{"key":"10.1016\/j.bbrc.2014.01.083_b0030","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.2337\/diabetes.54.4.1009","article-title":"PDX-1\/VP16 fusion protein, together with NeuroD or Ngn3, markedly induces insulin gene transcription and ameliorates glucose tolerance","volume":"54","author":"Kaneto","year":"2005","journal-title":"Diabetes"},{"key":"10.1016\/j.bbrc.2014.01.083_b0035","doi-asserted-by":"crossref","first-page":"15336","DOI":"10.1073\/pnas.1201701109","article-title":"In vivo reprogramming of Sox9+ cells in the liver to insulin-secreting ducts","volume":"109","author":"Banga","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bbrc.2014.01.083_b0040","doi-asserted-by":"crossref","first-page":"1863","DOI":"10.2337\/db09-0390","article-title":"Chronology of islet differentiation revealed by temporal cell labeling","volume":"58","author":"Miyatsuka","year":"2009","journal-title":"Diabetes"},{"key":"10.1016\/j.bbrc.2014.01.083_b0045","doi-asserted-by":"crossref","first-page":"E177","DOI":"10.1152\/ajpendo.00321.2002","article-title":"Transgenic mice with green fluorescent protein-labeled pancreatic beta-cells","volume":"284","author":"Hara","year":"2003","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"10.1016\/j.bbrc.2014.01.083_b0050","doi-asserted-by":"crossref","first-page":"2509","DOI":"10.1073\/pnas.95.5.2509","article-title":"A simplified system for generating recombinant adenoviruses","volume":"95","author":"He","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bbrc.2014.01.083_b0055","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1038\/nprot.2007.132","article-title":"Gene splicing and mutagenesis by PCR-driven overlap extension","volume":"2","author":"Heckman","year":"2007","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.bbrc.2014.01.083_b0060","first-page":"671","article-title":"Murine pancreatic ductal adenocarcinoma produced by in vitro transduction of polyoma middle T oncogene into the islets of Langerhans","volume":"145","author":"Yoshida","year":"1994","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.bbrc.2014.01.083_b0065","doi-asserted-by":"crossref","first-page":"13245","DOI":"10.1073\/pnas.0405301101","article-title":"Proendocrine genes coordinate the pancreatic islet differentiation program in vitro","volume":"101","author":"Gasa","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.bbrc.2014.01.083_b0070","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1038\/nbt957","article-title":"Correction of multi-gene deficiency in vivo using a single \u2018self-cleaving\u2019 2A peptide-based retroviral vector","volume":"22","author":"Szymczak","year":"2004","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.bbrc.2014.01.083_b0075","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1042\/BJ20111678","article-title":"Reprogramming of pancreatic exocrine cells towards a beta (beta) cell character using Pdx1, Ngn3 and MafA","volume":"442","author":"Akinci","year":"2012","journal-title":"Biochem. J."},{"key":"10.1016\/j.bbrc.2014.01.083_b0080","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1101\/gad.1412806","article-title":"Persistent expression of PDX-1 in the pancreas causes acinar-to-ductal metaplasia through Stat3 activation","volume":"20","author":"Miyatsuka","year":"2006","journal-title":"Genes Dev."},{"key":"10.1016\/j.bbrc.2014.01.083_b0085","doi-asserted-by":"crossref","first-page":"3533","DOI":"10.1242\/dev.127.16.3533","article-title":"Expression of neurogenin3 reveals an islet cell precursor population in the pancreas","volume":"127","author":"Schwitzgebel","year":"2000","journal-title":"Development"},{"key":"10.1016\/j.bbrc.2014.01.083_b0090","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.ydbio.2009.06.029","article-title":"Expression of MafA in pancreatic progenitors is detrimental for pancreatic development","volume":"333","author":"Nishimura","year":"2009","journal-title":"Dev. Biol."},{"key":"10.1016\/j.bbrc.2014.01.083_b0095","doi-asserted-by":"crossref","first-page":"2930","DOI":"10.1073\/pnas.0306233101","article-title":"The MafA transcription factor appears to be responsible for tissue-specific expression of insulin","volume":"101","author":"Matsuoka","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X14001077?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X14001077?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T19:38:53Z","timestamp":1779305933000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X14001077"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,2]]},"references-count":19,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,2]]}},"alternative-id":["S0006291X14001077"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2014.01.083","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2014,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Sequential introduction and dosage balance of defined transcription factors affect reprogramming efficiency from pancreatic duct cells into insulin-producing cells","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2014.01.083","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}