{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T22:46:38Z","timestamp":1789166798525,"version":"build-2803163510"},"reference-count":34,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Key Laboratory of Liaoning Educational Council","award":["L2013025"],"award-info":[{"award-number":["L2013025"]}]},{"name":"Central University","award":["DUT15QY43"],"award-info":[{"award-number":["DUT15QY43"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2016,9]]},"DOI":"10.1016\/j.bbrc.2016.08.109","type":"journal-article","created":{"date-parts":[[2016,8,23]],"date-time":"2016-08-23T05:13:30Z","timestamp":1471929210000},"page":"1268-1273","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":49,"title":["Cysteinome: The first comprehensive database for proteins with targetable cysteine and their covalent inhibitors"],"prefix":"10.1016","volume":"478","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4554-4610","authenticated-orcid":false,"given":"Sijin","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huizhe","family":"Luo (Howard)","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haina","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijie","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiwan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongliang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2016.08.109_bib1","doi-asserted-by":"crossref","first-page":"8965","DOI":"10.1021\/bi973101r","article-title":"Ionization-reactivity relationships for cysteine thiols in polypeptides","volume":"37","author":"Bulaj","year":"1998","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbrc.2016.08.109_bib2","doi-asserted-by":"crossref","DOI":"10.1186\/gb-2002-3-5-research0025","article-title":"Lateral gene transfer and parallel evolution in the history of glutathione biosynthesis genes","volume":"3","author":"Copley","year":"2002","journal-title":"Genome Biol."},{"key":"10.1016\/j.bbrc.2016.08.109_bib3","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1093\/jn\/117.6.1003","article-title":"Pharmacologic role of cysteine in ameliorating or exacerbating mineral toxicities","volume":"117","author":"Baker","year":"1987","journal-title":"J.\u00a0Nutr."},{"key":"10.1016\/j.bbrc.2016.08.109_bib4","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1146\/annurev.cellbio.22.010305.104538","article-title":"Iron-sulfur protein biogenesis in eukaryotes: components and mechanisms","volume":"22","author":"Lill","year":"2006","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"10.1016\/j.bbrc.2016.08.109_bib5","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1038\/nrm954","article-title":"Formation and transfer of disulphide bonds in living cells","volume":"3","author":"Sevier","year":"2002","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.bbrc.2016.08.109_bib6","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1002\/asia.200800427","article-title":"Chemical modification of proteins at cysteine: opportunities in chemistry and biology","volume":"4","author":"Chalker","year":"2009","journal-title":"Chem. Asian J."},{"key":"10.1016\/j.bbrc.2016.08.109_bib7","doi-asserted-by":"crossref","first-page":"1949","DOI":"10.1111\/j.1742-4658.2008.06347.x","article-title":"Chemical approaches to mapping the function of post-translational modifications","volume":"275","author":"Gamblin","year":"2008","journal-title":"Febs J."},{"key":"10.1016\/j.bbrc.2016.08.109_bib8","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1021\/jm101396q","article-title":"Cysteine mapping in conformationally distinct kinase nucleotide binding sites: application to the design of selective covalent inhibitors","volume":"54","author":"Leproult","year":"2011","journal-title":"J.\u00a0Med. Chem."},{"key":"10.1016\/j.bbrc.2016.08.109_bib9","doi-asserted-by":"crossref","first-page":"13075","DOI":"10.1073\/pnas.1004594107","article-title":"The Bruton tyrosine kinase inhibitor PCI-32765 blocks B-cell activation and is efficacious in models of autoimmune disease and B-cell malignancy","volume":"107","author":"Honigberg","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.bbrc.2016.08.109_bib10","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1002\/cmdc.200600221","article-title":"Discovery of selective irreversible inhibitors for Bruton's tyrosine kinase","volume":"2","author":"Pan","year":"2007","journal-title":"ChemMedChem"},{"key":"10.1016\/j.bbrc.2016.08.109_bib11","doi-asserted-by":"crossref","first-page":"20836","DOI":"10.1074\/jbc.M702615200","article-title":"Inhibition of the activation of multiple serine proteases with a cathepsin C inhibitor requires sustained exposure to prevent pro-enzyme processing","volume":"282","author":"Methot","year":"2007","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bbrc.2016.08.109_bib12","doi-asserted-by":"crossref","first-page":"2975","DOI":"10.1016\/j.bmc.2013.03.062","article-title":"Development of cell-active non-peptidyl inhibitors of cysteine cathepsins","volume":"21","author":"Dana","year":"2013","journal-title":"Bioorg. Med. Chem."},{"key":"10.1016\/j.bbrc.2016.08.109_bib13","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1007\/s10495-009-0388-9","article-title":"Conformational similarity in the activation of caspase-3 and -7 revealed by the unliganded and inhibited structures of caspase-7","volume":"14","author":"Agniswamy","year":"2009","journal-title":"Apoptosis"},{"key":"10.1016\/j.bbrc.2016.08.109_bib14","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1055\/s-0037-1614133","article-title":"Identification and biological activity of the active metabolite of clopidogrel","volume":"84","author":"Savi","year":"2000","journal-title":"Thromb. Haemost."},{"key":"10.1016\/j.bbrc.2016.08.109_bib15","first-page":"81","article-title":"Gastric acid secretion: activation and inhibition","volume":"67","author":"Sachs","year":"1994","journal-title":"Yale J. Biol. Med."},{"key":"10.1016\/j.bbrc.2016.08.109_bib16","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1038\/nrd1010","article-title":"A\u00a0proton-pump inhibitor expedition: the case histories of omeprazole and esomeprazole","volume":"2","author":"Olbe","year":"2003","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.bbrc.2016.08.109_bib17","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.bmcl.2013.10.003","article-title":"Drug discovery considerations in the development of covalent inhibitors","volume":"24","author":"Mah","year":"2014","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"10.1016\/j.bbrc.2016.08.109_bib18","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1038\/nrd3410","article-title":"The resurgence of covalent drugs","volume":"10","author":"Singh","year":"2011","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.bbrc.2016.08.109_bib19","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1038\/nature13393","article-title":"Targeting transcription regulation in cancer with a covalent CDK7 inhibitor","volume":"511","author":"Kwiatkowski","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.bbrc.2016.08.109_bib20","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.chembiol.2012.12.006","article-title":"Developing irreversible inhibitors of the protein kinase cysteinome","volume":"20","author":"Liu","year":"2013","journal-title":"Chem. Biol."},{"key":"10.1016\/j.bbrc.2016.08.109_bib21","doi-asserted-by":"crossref","first-page":"15046","DOI":"10.1073\/pnas.1111239108","article-title":"Chemical genetic strategy for targeting protein kinases based on covalent complementarity","volume":"108","author":"Garske","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.bbrc.2016.08.109_bib22","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1021\/jm901069a","article-title":"Identification and optimization of inhibitors of Trypanosomal cysteine proteases: cruzain, rhodesain, and TbCatB","volume":"53","author":"Mott","year":"2010","journal-title":"J.\u00a0Med. Chem."},{"key":"10.1016\/j.bbrc.2016.08.109_bib23","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1093\/nar\/28.1.235","article-title":"The protein Data Bank","volume":"28","author":"Berman","year":"2000","journal-title":"Nucl. Acids Res."},{"key":"10.1016\/j.bbrc.2016.08.109_bib24","series-title":"Annual Reports in Computational Chemistry","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/S1574-1400(08)00012-1","article-title":"Chapter 12-PubChem: integrated platform of small molecules and biological activities","author":"Bolton","year":"2008"},{"key":"10.1016\/j.bbrc.2016.08.109_bib25","doi-asserted-by":"crossref","first-page":"D355","DOI":"10.1093\/nar\/gkp896","article-title":"KEGG for representation and analysis of molecular networks involving diseases and drugs","volume":"38","author":"Kanehisa","year":"2010","journal-title":"Nucl. Acids Res."},{"key":"10.1016\/j.bbrc.2016.08.109_bib26","doi-asserted-by":"crossref","first-page":"D190","DOI":"10.1093\/nar\/gkn141","volume":"36","author":"The universal protein resource (UniProt)","year":"2008","journal-title":"Nucl. Acids Res."},{"key":"10.1016\/j.bbrc.2016.08.109_bib27","doi-asserted-by":"crossref","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","article-title":"Version 2.0","volume":"23","author":"Larkin","year":"2007","journal-title":"Bioinformatics"},{"key":"10.1016\/j.bbrc.2016.08.109_bib28","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. E"},{"key":"10.1016\/j.bbrc.2016.08.109_bib29","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1007\/s10930-014-9564-z","article-title":"Protein flexibility and cysteine reactivity: influence of mobility on the H-bond network and effects on pKa prediction","volume":"33","author":"Marino","year":"2014","journal-title":"Protein J."},{"key":"10.1016\/j.bbrc.2016.08.109_bib30","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1002\/prot.20660","article-title":"Very fast empirical prediction and rationalization of protein pKa values","volume":"61","author":"Li","year":"2005","journal-title":"Proteins"},{"key":"10.1016\/j.bbrc.2016.08.109_bib31","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1021\/ct100578z","article-title":"PROPKA3: consistent treatment of internal and surface residues in empirical pKa predictions","volume":"7","author":"Olsson","year":"2011","journal-title":"J.\u00a0Chem. Theory Comput."},{"key":"10.1016\/j.bbrc.2016.08.109_bib32","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1089\/cmb.2013.0071","article-title":"Protein depth calculation and the use for improving accuracy of protein fold recognition","volume":"20","author":"Xu","year":"2013","journal-title":"J.\u00a0Comput. Biol."},{"key":"10.1016\/j.bbrc.2016.08.109_bib33","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0022-2836(82)90515-0","article-title":"A\u00a0simple method for displaying the hydropathic character of a protein","volume":"157","author":"Kyte","year":"1982","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.bbrc.2016.08.109_bib34","doi-asserted-by":"crossref","first-page":"6404","DOI":"10.1021\/ja710254m","article-title":"Identification of a new class of nonpeptidic inhibitors of cruzain","volume":"130","author":"Brak","year":"2008","journal-title":"J.\u00a0Am. Chem. Soc."}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X16313651?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X16313651?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,7,14]],"date-time":"2018-07-14T00:58:00Z","timestamp":1531529880000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X16313651"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9]]},"references-count":34,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,9]]}},"alternative-id":["S0006291X16313651"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2016.08.109","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2016,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cysteinome: The first comprehensive database for proteins with targetable cysteine and their covalent inhibitors","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2016.08.109","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}