{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T13:10:54Z","timestamp":1769692254063,"version":"3.49.0"},"reference-count":33,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2018,9,1]],"date-time":"2018-09-01T00:00:00Z","timestamp":1535760000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,9,1]],"date-time":"2018-09-01T00:00:00Z","timestamp":1535760000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2018,9]]},"DOI":"10.1016\/j.bbrc.2018.07.131","type":"journal-article","created":{"date-parts":[[2018,7,29]],"date-time":"2018-07-29T01:43:28Z","timestamp":1532828608000},"page":"1885-1890","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"title":["In\u00a0vitro analysis of drugs that improve hyperglycemia-induced blood-brain barrier dysfunction"],"prefix":"10.1016","volume":"503","author":[{"given":"Ryoma","family":"Kayano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7135-2220","authenticated-orcid":false,"given":"Yoichi","family":"Morofuji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7945-9719","authenticated-orcid":false,"given":"Shinsuke","family":"Nakagawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuji","family":"Fukuda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Ozawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masami","family":"Niwa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayuki","family":"Matsuo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2018.07.131_bib1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.nbd.2009.07.030","article-title":"Structure and function of the blood-brain barrier","volume":"37","author":"Abbott","year":"2010","journal-title":"Neurobiol. Dis."},{"key":"10.1016\/j.bbrc.2018.07.131_bib2","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.neuron.2008.01.003","article-title":"The blood-brain barrier in health and chronic neurodegenerative disorders","volume":"57","author":"Zlokovic","year":"2008","journal-title":"Neuron"},{"key":"10.1016\/j.bbrc.2018.07.131_bib3","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1007\/s00125-006-0485-z","article-title":"Increased blood-brain barrier permeability and altered tight junctions in experimental diabetes in the rat: contribution of hyperglycaemia and matrix metalloproteinases","volume":"50","author":"Hawkins","year":"2007","journal-title":"Diabetologia"},{"key":"10.1016\/j.bbrc.2018.07.131_bib4","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1253\/circj.CJ-11-0121","article-title":"Neurovascular coupling in cognitive impairment associated with diabetes mellitus","volume":"75","author":"Mogi","year":"2011","journal-title":"Circ. J."},{"key":"10.1016\/j.bbrc.2018.07.131_bib5","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1038\/414813a","article-title":"Biochemistry and molecular cell biology of diabetic complications","volume":"414","author":"Brownlee","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.bbrc.2018.07.131_bib6","first-page":"125","article-title":"Diabetes mellitus and blood-brain barrier dysfunction: an overview","volume":"2","author":"Prasad","year":"2014","journal-title":"J\u00a0Pharmacovigil"},{"key":"10.1016\/j.bbrc.2018.07.131_bib7","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/S1474-4422(10)70198-8","article-title":"group, Cilostazol for prevention of secondary stroke (CSPS 2): an aspirin-controlled, double-blind, randomised non-inferiority trial","volume":"9","author":"Shinohara","year":"2010","journal-title":"Lancet Neurol."},{"key":"10.1016\/j.bbrc.2018.07.131_bib8","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.jns.2011.09.012","article-title":"The factors associated with a functional outcome after ischemic stroke in diabetic patients: the Fukuoka Stroke Registry","volume":"313","author":"Kuwashiro","year":"2012","journal-title":"J.\u00a0Neurol. Sci."},{"key":"10.1016\/j.bbrc.2018.07.131_bib9","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.brainres.2013.04.046","article-title":"Therapeutic impact of eicosapentaenoic acid on ischemic brain damage following transient focal cerebral ischemia in rats","volume":"1519","author":"Ueda","year":"2013","journal-title":"Brain Res."},{"key":"10.1016\/j.bbrc.2018.07.131_bib10","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1161\/STROKEAHA.107.501361","article-title":"Statin therapy for stroke prevention","volume":"39","author":"Nassief","year":"2008","journal-title":"Stroke"},{"key":"10.1016\/j.bbrc.2018.07.131_bib11","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1007\/s10571-007-9195-4","article-title":"Pericytes from brain microvessels strengthen the barrier integrity in primary cultures of rat brain endothelial cells","volume":"27","author":"Nakagawa","year":"2007","journal-title":"Cell. Mol. Neurobiol."},{"key":"10.1016\/j.bbrc.2018.07.131_bib12","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.neuint.2008.12.002","article-title":"A\u00a0new blood-brain barrier model using primary rat brain endothelial cells, pericytes and astrocytes","volume":"54","author":"Nakagawa","year":"2009","journal-title":"Neurochem. Int."},{"key":"10.1016\/j.bbrc.2018.07.131_bib13","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1016\/S1359-6446(03)02873-3","article-title":"In\u00a0vitro trans-monolayer permeability calculations: often forgotten assumptions","volume":"8","author":"Youdim","year":"2003","journal-title":"Drug Discov. Today"},{"key":"10.1016\/j.bbrc.2018.07.131_bib14","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1111\/j.1549-8719.2010.00072.x","article-title":"Beta-adrenergic stimulation contributes to maintenance of endothelial barrier functions under baseline conditions","volume":"18","author":"Spindler","year":"2011","journal-title":"Microcirculation (N. Y.)"},{"key":"10.1016\/j.bbrc.2018.07.131_bib15","doi-asserted-by":"crossref","first-page":"1928","DOI":"10.1038\/jcbfm.2013.151","article-title":"PKC-beta exacerbates in\u00a0vitro brain barrier damage in hyperglycemic settings via regulation of RhoA\/Rho-kinase\/MLC2 pathway","volume":"33","author":"Srivastava","year":"2013","journal-title":"J.\u00a0Cerebr. Blood Flow Metabol."},{"key":"10.1016\/j.bbrc.2018.07.131_bib16","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1136\/jnnp.74.1.70","article-title":"Increased blood-brain barrier permeability in type II diabetes demonstrated by gadolinium magnetic resonance imaging","volume":"74","author":"Starr","year":"2003","journal-title":"J.\u00a0Neurol. Neurosurg. Psychiatry"},{"key":"10.1016\/j.bbrc.2018.07.131_bib17","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1016\/j.redox.2014.05.005","article-title":"Hyperglycaemia promotes human brain microvascular endothelial cell apoptosis via induction of protein kinase C-ssI and prooxidant enzyme NADPH oxidase","volume":"2","author":"Shao","year":"2014","journal-title":"Redox Biol"},{"key":"10.1016\/j.bbrc.2018.07.131_bib18","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1007\/s001250050900","article-title":"Introduction of apoptosis by high proinsulin and glucose in cultured human umbilical vein endothelial cells is mediated by reactive oxygen species","volume":"41","author":"Du","year":"1998","journal-title":"Diabetologia"},{"key":"10.1016\/j.bbrc.2018.07.131_bib19","doi-asserted-by":"crossref","first-page":"1472","DOI":"10.2337\/diabetes.50.6.1472","article-title":"Phosphorylation of p38 mitogen-activated protein kinase downstream of bax-caspase-3 pathway leads to cell death induced by high D-glucose in human endothelial cells","volume":"50","author":"Nakagami","year":"2001","journal-title":"Diabetes"},{"key":"10.1016\/j.bbrc.2018.07.131_bib20","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1172\/JCI114988","article-title":"Diacylglycerol accumulation and microvascular abnormalities induced by elevated glucose levels","volume":"87","author":"Wolf","year":"1991","journal-title":"J.\u00a0Clin. Invest."},{"key":"10.1016\/j.bbrc.2018.07.131_bib21","doi-asserted-by":"crossref","first-page":"1721","DOI":"10.1016\/j.lfs.2007.02.001","article-title":"Induction of reactive oxygen species from isolated rat glomeruli by protein kinase C activation and TNF-alpha stimulation, and effects of a phosphodiesterase inhibitor","volume":"80","author":"Koike","year":"2007","journal-title":"Life Sci."},{"key":"10.1016\/j.bbrc.2018.07.131_bib22","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/s00018-011-0731-5","article-title":"HIF-1 is involved in high glucose-induced paracellular permeability of brain endothelial cells","volume":"69","author":"Yan","year":"2012","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.bbrc.2018.07.131_bib23","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/2045-8118-11-8","article-title":"Impact of altered glycaemia on blood-brain barrier endothelium: an in\u00a0vitro study using the hCMEC\/D3 cell line","volume":"11","author":"Sajja","year":"2014","journal-title":"Fluids Barriers CNS"},{"key":"10.1016\/j.bbrc.2018.07.131_bib24","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1111\/j.1463-1326.2008.00987.x","article-title":"Antioxidants attenuate hyperglycaemia-mediated brain endothelial cell dysfunction and blood-brain barrier hyperpermeability","volume":"11","author":"Allen","year":"2009","journal-title":"Diabetes Obes. Metabol."},{"key":"10.1016\/j.bbrc.2018.07.131_bib25","doi-asserted-by":"crossref","first-page":"166","DOI":"10.3389\/fnmol.2018.00166","article-title":"Comparison of a rat primary cell-based blood-brain barrier model with epithelial and brain endothelial cell lines: gene expression and drug transport","volume":"11","author":"Veszelka","year":"2018","journal-title":"Front. Mol. Neurosci."},{"key":"10.1016\/j.bbrc.2018.07.131_bib26","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/s10571-004-1377-8","article-title":"Permeability studies on in\u00a0vitro blood-brain barrier models: physiology, pathology, and pharmacology","volume":"25","author":"Deli","year":"2005","journal-title":"Cell. Mol. Neurobiol."},{"key":"10.1016\/j.bbrc.2018.07.131_bib27","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1007\/s10571-010-9497-9","article-title":"Pitavastatin strengthens the barrier integrity in primary cultures of rat brain endothelial cells","volume":"30","author":"Morofuji","year":"2010","journal-title":"Cell. Mol. Neurobiol."},{"key":"10.1016\/j.bbrc.2018.07.131_bib28","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.ejphar.2007.04.065","article-title":"Low-dose treatment with atorvastatin leads to anti-oxidative and anti-inflammatory effects in diabetes mellitus","volume":"569","author":"Riad","year":"2007","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.bbrc.2018.07.131_bib29","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.phrs.2017.06.017","article-title":"Beneficial effects of Angiotensin II receptor blockers in brain disorders","volume":"125","author":"Saavedra","year":"2017","journal-title":"Pharmacol. Res."},{"key":"10.1016\/j.bbrc.2018.07.131_bib30","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1007\/s10571-014-0152-8","article-title":"Candesartan improves ischemia-induced impairment of the blood-brain barrier in\u00a0vitro","volume":"35","author":"So","year":"2015","journal-title":"Cell. Mol. Neurobiol."},{"key":"10.1016\/j.bbrc.2018.07.131_bib31","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/s10571-012-9896-1","article-title":"Cilostazol strengthens barrier integrity in brain endothelial cells","volume":"33","author":"Horai","year":"2013","journal-title":"Cell. Mol. Neurobiol."},{"key":"10.1016\/j.bbrc.2018.07.131_bib32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mcn.2014.01.006","article-title":"Cilostazol attenuates ischemia-reperfusion-induced blood-brain barrier dysfunction enhanced by advanced glycation endproducts via transforming growth factor-beta1 signaling","volume":"60","author":"Takeshita","year":"2014","journal-title":"Mol. Cell. Neurosci."},{"key":"10.1016\/j.bbrc.2018.07.131_bib33","doi-asserted-by":"crossref","first-page":"F1451","DOI":"10.1152\/ajprenal.00637.2009","article-title":"Eicosapentaenoic acid restores diabetic tubular injury through regulating oxidative stress and mitochondrial apoptosis","volume":"299","author":"Taneda","year":"2010","journal-title":"Am. J. Physiol. Ren. Physiol."}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X18316358?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X18316358?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T11:36:31Z","timestamp":1757763391000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X18316358"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,9]]}},"alternative-id":["S0006291X18316358"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2018.07.131","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2018,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"In\u00a0vitro analysis of drugs that improve hyperglycemia-induced blood-brain barrier dysfunction","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2018.07.131","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}