{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T06:21:19Z","timestamp":1773555679116,"version":"3.50.1"},"reference-count":26,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100004396","name":"Thailand Research Fund","doi-asserted-by":"publisher","award":["RSA5580047"],"award-info":[{"award-number":["RSA5580047"]}],"id":[{"id":"10.13039\/501100004396","id-type":"DOI","asserted-by":"publisher"}]},{"name":"TRF-Royal Golden Jubilee Ph.D. scholarship"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2019,6]]},"DOI":"10.1016\/j.bbrc.2019.04.096","type":"journal-article","created":{"date-parts":[[2019,4,27]],"date-time":"2019-04-27T07:29:54Z","timestamp":1556350194000},"page":"365-371","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"title":["Importance of the Cys124\u2212Cys128 intermolecular disulfide bonding for oligomeric assembly and hemolytic activity of the Helicobacter pylori TlyA hemolysin"],"prefix":"10.1016","volume":"514","author":[{"given":"Nitchakan","family":"Samainukul","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aung Khine","family":"Linn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Bagher","family":"Javadi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Somsri","family":"Sakdee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chanan","family":"Angsuthanasombat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerd","family":"Katzenmeier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2019.04.096_bib1","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1128\/CMR.00011-10","article-title":"Helicobacter pylori and gastric cancer: factors that modulate disease risk","volume":"23","author":"Wroblewski","year":"2010","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.bbrc.2019.04.096_bib2","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.toxicon.2016.04.037","article-title":"Vacuolating cytotoxin A (VacA) - a multi-talented pore-forming toxin from Helicobacter pylori","volume":"118","author":"Junaid","year":"2016","journal-title":"Toxicon"},{"key":"10.1016\/j.bbrc.2019.04.096_bib3","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/41483","article-title":"The complete genome sequence of the gastric pathogen Helicobacter pylori","volume":"388","author":"Tomb","year":"1997","journal-title":"Nature"},{"key":"10.1016\/j.bbrc.2019.04.096_bib4","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.molcel.2006.05.044","article-title":"Capreomycin binds across the ribosomal subunit interface using tlyA-encoded 2'-O-methylations in 16S and 23S rRNAs","volume":"23","author":"Johansen","year":"2006","journal-title":"Mol. Cell"},{"key":"10.1016\/j.bbrc.2019.04.096_bib5","doi-asserted-by":"crossref","first-page":"1697","DOI":"10.1128\/IAI.69.3.1697-1703.2001","article-title":"Helicobacter pylori pore-forming cytolysin orthologue TlyA possesses in\u00a0vitro hemolytic activity and has a role in colonization of the gastric mucosa","volume":"69","author":"Martino","year":"2001","journal-title":"Infect. Immun."},{"key":"10.1016\/j.bbrc.2019.04.096_bib6","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.bbrc.2014.01.011","article-title":"Functional characterization of Helicobacter pylori TlyA: pore-forming hemolytic activity and cytotoxic property of the protein","volume":"444","author":"Lata","year":"2014","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2019.04.096_bib7","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1097\/00042737-200012120-00002","article-title":"Iron influences the expression of Helicobacter pylori outer membrane vesicle-associated virulence factors","volume":"12","author":"Keenan","year":"2000","journal-title":"Eur. J. Gastroenterol. Hepatol."},{"key":"10.1016\/j.bbrc.2019.04.096_bib8","doi-asserted-by":"crossref","first-page":"3553","DOI":"10.1021\/bi500152n","article-title":"Helicobacter pylori TlyA agglutinates liposomes and induces fusion and permeabilization of the liposome membranes","volume":"53","author":"Lata","year":"2014","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbrc.2019.04.096_bib9","doi-asserted-by":"crossref","first-page":"W315","DOI":"10.1093\/nar\/gky497","article-title":"BeStSel: a web server for accurate protein secondary structure prediction and fold recognition from the circular dichroism spectra","volume":"46","author":"Micsonai","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.bbrc.2019.04.096_bib10","doi-asserted-by":"crossref","first-page":"20793","DOI":"10.1074\/jbc.M114.627554","article-title":"Potential prepore trimer formation by the Bacillus thuringiensis mosquito-specific toxin: molecular insights into a critical prerequisite of membrane-bound monomers","volume":"290","author":"Sriwimol","year":"2015","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bbrc.2019.04.096_bib11","doi-asserted-by":"crossref","first-page":"109","DOI":"10.3390\/toxins9030109","article-title":"Functional contributions of positive charges in the pore-lining helix 3 of the Bordetella pertussis CyaA-hemolysin to hemolytic activity and ion-channel opening","volume":"9","author":"Kurehong","year":"2017","journal-title":"Toxins"},{"key":"10.1016\/j.bbrc.2019.04.096_bib12","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0022-2836(81)90087-5","article-title":"Identification of common molecular subsequences","volume":"147","author":"Smith","year":"1981","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.bbrc.2019.04.096_bib13","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1002\/prot.340230306","article-title":"Evaluation of comparative protein modeling by MODELLER","volume":"23","author":"Sali","year":"1995","journal-title":"Proteins"},{"key":"10.1016\/j.bbrc.2019.04.096_bib14","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1016\/S0969-2126(97)00260-8","article-title":"CATH: a hierarchic classification of protein domain structures","volume":"5","author":"Orengo","year":"1997","journal-title":"Structure"},{"key":"10.1016\/j.bbrc.2019.04.096_bib15","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1002\/pro.3330","article-title":"MolProbity: more and better reference data for improved all-atom structure validation","volume":"27","author":"Williams","year":"2018","journal-title":"Protein Sci."},{"key":"10.1016\/j.bbrc.2019.04.096_bib16","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1107\/S0021889891004399","article-title":"MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures","volume":"24","author":"Kraulis","year":"1991","journal-title":"J.\u00a0Appl. Crystallogr."},{"key":"10.1016\/j.bbrc.2019.04.096_bib17","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/S0968-0004(03)00090-2","article-title":"Many paths to methyltransfer: a chronicle of convergence","volume":"28","author":"Schubert","year":"2003","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.bbrc.2019.04.096_bib18","doi-asserted-by":"crossref","first-page":"3000","DOI":"10.1021\/ol901015a","article-title":"Use of disulfide \"staples\" to stabilize beta-sheet quaternary structure","volume":"11","author":"Khakshoor","year":"2009","journal-title":"Org. Lett."},{"key":"10.1016\/j.bbrc.2019.04.096_bib19","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/S0968-0004(03)00057-4","article-title":"Disulfide bonds as switches for protein function","volume":"28","author":"Hogg","year":"2003","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.bbrc.2019.04.096_bib20","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/1471-2091-11-35","article-title":"Molecular characterization of tlyA gene product, Rv1694 of Mycobacterium tuberculosis: a non-conventional hemolysin and a ribosomal RNA methyl transferase","volume":"11","author":"Rahman","year":"2010","journal-title":"BMC Biochem."},{"key":"10.1016\/j.bbrc.2019.04.096_bib21","doi-asserted-by":"crossref","first-page":"27598","DOI":"10.1074\/jbc.M804231200","article-title":"Structure and evolution of a novel dimeric enzyme from a clinically important bacterial pathogen","volume":"283","author":"Burgess","year":"2008","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bbrc.2019.04.096_bib22","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0079778","article-title":"Novel disulfide bond-mediated dimerization of the CARD domain was revealed by the crystal structure of CARMA1 CARD","volume":"8","author":"Jang","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.bbrc.2019.04.096_bib23","doi-asserted-by":"crossref","first-page":"14250","DOI":"10.1073\/pnas.231465798","article-title":"Dimer formation drives the activation of the cell death protease caspase 9","volume":"98","author":"Renatus","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.bbrc.2019.04.096_bib24","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1038\/nbt1029","article-title":"Recombinant protein folding and misfolding in Escherichia coli","volume":"22","author":"Baneyx","year":"2004","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.bbrc.2019.04.096_bib25","doi-asserted-by":"crossref","first-page":"60","DOI":"10.3389\/fcimb.2015.00060","article-title":"Mycobacterial tlyA gene product is localized to the cell-wall without signal sequence","volume":"5","author":"Kumar","year":"2015","journal-title":"Front. Cell Infect. Microbiol."},{"key":"10.1016\/j.bbrc.2019.04.096_bib26","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0065-3233(08)60520-3","article-title":"The anatomy and taxonomy of protein structure","volume":"34","author":"Richardson","year":"1981","journal-title":"Adv. Protein Chem."}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X19307387?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X19307387?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T11:36:17Z","timestamp":1757763377000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X19307387"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,6]]}},"alternative-id":["S0006291X19307387"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2019.04.096","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2019,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Importance of the Cys124\u2212Cys128 intermolecular disulfide bonding for oligomeric assembly and hemolytic activity of the Helicobacter pylori TlyA hemolysin","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2019.04.096","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}