{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:53:05Z","timestamp":1762102385987,"version":"3.37.3"},"reference-count":22,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB 685"],"award-info":[{"award-number":["SFB 685"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2019,7]]},"DOI":"10.1016\/j.bbrc.2019.05.094","type":"journal-article","created":{"date-parts":[[2019,5,23]],"date-time":"2019-05-23T10:34:05Z","timestamp":1558607645000},"page":"145-148","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Pepstatin pull-down at high pH is a powerful tool for detection and analysis of napsin A"],"prefix":"10.1016","volume":"515","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2412-5361","authenticated-orcid":false,"given":"Andreas","family":"Maurer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hubert","family":"Kalbacher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2019.05.094_bib1","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1016\/j.bbrc.2008.10.162","article-title":"Cathepsin E regulates the presentation of tetanus toxin C-fragment in PMA activated primary human B cells","volume":"377","author":"Burster","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2019.05.094_bib2","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/S0014-5793(99)01458-1","article-title":"Human napsin A: expression, immunochemical detection, and tissue localization","volume":"462","author":"Schauer-Vukasinovic","year":"1999","journal-title":"FEBS Lett."},{"key":"10.1016\/j.bbrc.2019.05.094_bib3","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1183\/09031936.00081207","article-title":"Cathepsin H and napsin A are active in the alveoli and increased in alveolar proteinosis","volume":"31","author":"Woischnik","year":"2008","journal-title":"Eur. Respir. J."},{"key":"10.1016\/j.bbrc.2019.05.094_bib4","doi-asserted-by":"crossref","first-page":"49006","DOI":"10.1074\/jbc.M306844200","article-title":"Involvement of napsin A in the C- and N-terminal processing of surfactant protein B in type-II pneumocytes of the human lung","volume":"278","author":"Brasch","year":"2003","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bbrc.2019.05.094_bib5","doi-asserted-by":"crossref","first-page":"16178","DOI":"10.1074\/jbc.M312029200","article-title":"Processing of pulmonary surfactant protein B by napsin and cathepsin H","volume":"279","author":"Ueno","year":"2004","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bbrc.2019.05.094_bib6","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.bbrc.2008.10.034","article-title":"Emerging functional roles of cathepsin E","volume":"377","author":"Zaidi","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2019.05.094_bib7","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/j.bbrc.2007.12.163","article-title":"Cathepsin E: a mini review","volume":"367","author":"Zaidi","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2019.05.094_bib8","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.bbrc.2008.08.099","article-title":"Cathepsin D: a cellular roadmap","volume":"376","author":"Zaidi","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2019.05.094_bib9","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.bbrc.2007.05.187","article-title":"Recombinant cathepsin E has no proteolytic activity at neutral pH","volume":"360","author":"Zaidi","year":"2007","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2019.05.094_bib10","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1038\/2306","article-title":"Conformational switching in an aspartic proteinase","volume":"5","author":"Lee","year":"1998","journal-title":"Nat. Struct. Biol."},{"key":"10.1016\/j.bbrc.2019.05.094_bib11","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/0006-291X(90)91356-W","article-title":"Affinity purification of HIV-1 and HIV-2 proteases from recombinant E. coli strains using pepstatin-agarose","volume":"171","author":"Rittenhouse","year":"1990","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2019.05.094_bib12","first-page":"1107","article-title":"A\u00a0rapid and large-scale isolation of renin from mouse submaxillary gland by pepstatin-aminohexyl-agarose affinity chromatography","volume":"89","author":"Suzuki","year":"1981","journal-title":"J.\u00a0Biochem."},{"key":"10.1016\/j.bbrc.2019.05.094_bib13","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0014-5793(99)01493-3","article-title":"Napsin A, a member of the aspartic protease family, is abundantly expressed in normal lung and kidney tissue and is expressed in lung adenocarcinomas","volume":"462","author":"Chuman","year":"1999","journal-title":"FEBS Lett."},{"key":"10.1016\/j.bbrc.2019.05.094_bib14","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1111\/j.1349-7006.2000.tb00879.x","article-title":"Human tissue distribution of TA02, which is homologous with a new type of aspartic proteinase, napsin A","volume":"91","author":"Hirano","year":"2000","journal-title":"Jpn. J. Cancer Res."},{"key":"10.1016\/j.bbrc.2019.05.094_bib15","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0169-5002(03)00194-6","article-title":"Usefulness of TA02 (napsin A) to distinguish primary lung adenocarcinoma from metastatic lung adenocarcinoma","volume":"41","author":"Hirano","year":"2003","journal-title":"Lung Cancer"},{"key":"10.1016\/j.bbrc.2019.05.094_bib16","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1097\/PAP.0b013e31823e472e","article-title":"Napsin A expression in lung and kidney neoplasia: a review and update","volume":"19","author":"Ordonez","year":"2012","journal-title":"Adv. Anat. Pathol."},{"key":"10.1016\/j.bbrc.2019.05.094_bib17","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.prp.2005.05.010","article-title":"Napsin A is useful to distinguish primary lung adenocarcinoma from adenocarcinomas of other organs","volume":"201","author":"Suzuki","year":"2005","journal-title":"Pathol. Res. Pract."},{"key":"10.1016\/j.bbrc.2019.05.094_bib18","series-title":"Shedding Light on the Role of Aspartic Proteases in MHC Class II Antigen Processing","first-page":"124","author":"Maurer","year":"2014"},{"key":"10.1016\/j.bbrc.2019.05.094_bib19","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1679\/aohc.65.359","article-title":"Immunohistochemical localization of napsin and its potential role in protein catabolism in renal proximal tubules","volume":"65","author":"Mori","year":"2002","journal-title":"Arch. Histol. Cytol."},{"key":"10.1016\/j.bbrc.2019.05.094_bib20","doi-asserted-by":"crossref","first-page":"2573","DOI":"10.1046\/j.1432-1327.2000.01268.x","article-title":"Purification and characterization of active recombinant human napsin A","volume":"267","author":"Schauer-Vukasinovic","year":"2000","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.bbrc.2019.05.094_bib21","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1021\/pr300758c","article-title":"A\u00a0periodate-cleavable linker for functional proteomics under slightly acidic conditions: application for the analysis of intracellular aspartic proteases","volume":"12","author":"Maurer","year":"2013","journal-title":"J.\u00a0Proteome Res."},{"key":"10.1016\/j.bbrc.2019.05.094_bib22","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1002\/elps.1150080203","article-title":"Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels","volume":"8","author":"Blum","year":"1987","journal-title":"Electrophoresis"}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X19309702?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X19309702?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,8,6]],"date-time":"2019-08-06T17:43:31Z","timestamp":1565113411000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X19309702"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["S0006291X19309702"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2019.05.094","relation":{},"ISSN":["0006-291X"],"issn-type":[{"type":"print","value":"0006-291X"}],"subject":[],"published":{"date-parts":[[2019,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Pepstatin pull-down at high pH is a powerful tool for detection and analysis of napsin A","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2019.05.094","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}