{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T08:33:10Z","timestamp":1779352390036,"version":"3.51.4"},"reference-count":30,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,12,26]],"date-time":"2020-12-26T00:00:00Z","timestamp":1608940800000},"content-version":"am","delay-in-days":360,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100005725","name":"CHDI Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2020,1]]},"DOI":"10.1016\/j.bbrc.2019.09.097","type":"journal-article","created":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T21:52:30Z","timestamp":1572558750000},"page":"549-554","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"title":["Ultrasensitive quantitative measurement of huntingtin phosphorylation at residue S13"],"prefix":"10.1016","volume":"521","author":[{"given":"Cristina","family":"Cariulo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margherita","family":"Verani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Martufi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raffaele","family":"Ingenito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Finotto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sean M.","family":"Deguire","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel J.","family":"Lavery","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leticia","family":"Toledo-Sherman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramee","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth M.","family":"Doherty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas F.","family":"Vogt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celia","family":"Dominguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hilal A.","family":"Lashuel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lara","family":"Petricca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Caricasole","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2019.09.097_bib1","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S1474-4422(10)70245-3","article-title":"Huntington\u2019s disease: from molecular pathogenesis to clinical treatment","volume":"10","author":"Ross","year":"2011","journal-title":"Lancet Neurol."},{"key":"10.1016\/j.bbrc.2019.09.097_bib2","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/S0092-8674(00)81369-0","article-title":"Exon 1 of the HD gene with an expanded CAG repeat is sufficient to cause a progressive neurological phenotype in transgenic mice","volume":"87","author":"Mangiarini","year":"1996","journal-title":"Cell"},{"key":"10.1016\/j.bbrc.2019.09.097_bib3","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1083\/jcb.200306038","article-title":"Huntingtin forms toxic NH2-terminal fragment complexes that are promoted by the age-dependent decrease in proteasome activity","volume":"163","author":"Zhou","year":"2003","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.bbrc.2019.09.097_bib4","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1083\/jcb.200909067","article-title":"IKK phosphorylates Huntingtin and targets it for degradation by the proteasome and lysosome","volume":"187","author":"Thompson","year":"2009","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.bbrc.2019.09.097_bib5","doi-asserted-by":"crossref","first-page":"29427","DOI":"10.1074\/jbc.M109.013193","article-title":"Phosphorylation of threonine 3: implications for Huntingtin aggregation and neurotoxicity","volume":"284","author":"Aiken","year":"2009","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bbrc.2019.09.097_bib6","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1093\/hmg\/dds554","article-title":"The huntingtin N17 domain is a multifunctional CRM1 and Ran-dependent nuclear and cilial export signal","volume":"22","author":"Maiuri","year":"2013","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.bbrc.2019.09.097_bib7","doi-asserted-by":"crossref","first-page":"6063","DOI":"10.1074\/jbc.M112.413575","article-title":"An N-terminal nuclear export signal regulates trafficking and aggregation of Huntingtin (Htt) protein exon 1","volume":"288","author":"Zheng","year":"2013","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bbrc.2019.09.097_bib8","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1016\/j.bbrc.2015.06.116","article-title":"Detection of huntingtin exon 1 phosphorylation by Phos-Tag SDS-PAGE: predominant phosphorylation on threonine 3 and regulation by IKKbeta","volume":"463","author":"Bustamante","year":"2015","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2019.09.097_bib9","doi-asserted-by":"crossref","first-page":"E10809","DOI":"10.1073\/pnas.1705372114","article-title":"Phosphorylation of huntingtin at residue T3 is decreased in Huntington\u2019s disease and modulates mutant huntingtin protein conformation","volume":"114","author":"Cariulo","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.bbrc.2019.09.097_bib10","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1016\/j.neuron.2009.11.020","article-title":"Serines 13 and 16 are critical determinants of full-length human mutant huntingtin induced disease pathogenesis in HD mice","volume":"64","author":"Gu","year":"2009","journal-title":"Neuron"},{"key":"10.1016\/j.bbrc.2019.09.097_bib11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jmb.2012.09.011","article-title":"Serine phosphorylation suppresses huntingtin amyloid accumulation by altering protein aggregation properties","volume":"424","author":"Mishra","year":"2012","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.bbrc.2019.09.097_bib12","doi-asserted-by":"crossref","first-page":"1928","DOI":"10.1002\/anie.201307510","article-title":"One-pot semisynthesis of exon 1 of the Huntingtin protein: new tools for elucidating the role of posttranslational modifications in the pathogenesis of Huntington\u2019s disease","volume":"53","author":"Ansaloni","year":"2014","journal-title":"Angew Chem. Int. Ed. Engl."},{"issue":"19","key":"10.1016\/j.bbrc.2019.09.097_bib13","doi-asserted-by":"crossref","first-page":"5202","DOI":"10.1002\/anie.201611750","article-title":"Mutant Exon1 Huntingtin Aggregation is Regulated by T3 Phosphorylation-Induced Structural Changes and Crosstalk between T3 Phosphorylation and Acetylation at K6","volume":"56","author":"Chiki","year":"2017","journal-title":"Angew Chem. Int. Ed. Engl."},{"key":"10.1016\/j.bbrc.2019.09.097_bib14","doi-asserted-by":"crossref","first-page":"18540","DOI":"10.1074\/jbc.RA118.004621","article-title":"N-terminal Huntingtin (Htt) phosphorylation is a molecular switch regulating Htt aggregation, helical conformation, internalization, and nuclear targeting","volume":"293","author":"DeGuire","year":"2018","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bbrc.2019.09.097_bib15","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1038\/nchembio.582","article-title":"Kinase inhibitors modulate huntingtin cell localization and toxicity","volume":"7","author":"Atwal","year":"2011","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.bbrc.2019.09.097_bib16","doi-asserted-by":"crossref","first-page":"3528","DOI":"10.1073\/pnas.1114502109","article-title":"Ganglioside GM1 induces phosphorylation of mutant huntingtin and restores normal motor behavior in Huntington disease mice","volume":"109","author":"Di Pardo","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.bbrc.2019.09.097_bib17","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.15252\/emmm.201707763","article-title":"Disease-modifying effects of ganglioside GM1 in Huntington\u2019s disease models","volume":"9","author":"Alpaugh","year":"2017","journal-title":"EMBO Mol. Med."},{"key":"10.1016\/j.bbrc.2019.09.097_bib18","doi-asserted-by":"crossref","first-page":"E7081","DOI":"10.1073\/pnas.1801772115","article-title":"N6-Furfuryladenine is protective in Huntington\u2019s disease models by signaling huntingtin phosphorylation","volume":"115","author":"Bowie","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.bbrc.2019.09.097_bib19","doi-asserted-by":"crossref","first-page":"2692","DOI":"10.1021\/acs.jproteome.6b00991","article-title":"Post-translational modifications (PTMs), identified on endogenous huntingtin, cluster within proteolytic domains between HEAT repeats","volume":"16","author":"Ratovitski","year":"2017","journal-title":"J.\u00a0Proteome Res."},{"key":"10.1016\/j.bbrc.2019.09.097_bib20","doi-asserted-by":"crossref","first-page":"23686","DOI":"10.1074\/jbc.M513507200","article-title":"Huntingtin phosphorylation sites mapped by mass spectrometry. Modulation of cleavage and toxicity","volume":"281","author":"Schilling","year":"2006","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bbrc.2019.09.097_bib21","doi-asserted-by":"crossref","first-page":"1990","DOI":"10.1373\/clinchem.2007.091181","article-title":"Ultrasensitive flow-based immunoassays using single-molecule counting","volume":"53","author":"Todd","year":"2007","journal-title":"Clin. Chem."},{"key":"10.1016\/j.bbrc.2019.09.097_bib22","doi-asserted-by":"crossref","DOI":"10.1074\/mcp.M111.009829","article-title":"Mass spectrometric identification of novel lysine acetylation sites in huntingtin","volume":"10","author":"Cong","year":"2011","journal-title":"Mol. Cell. Proteom."},{"issue":"48","key":"10.1016\/j.bbrc.2019.09.097_bib23","doi-asserted-by":"crossref","first-page":"18540","DOI":"10.1074\/jbc.RA118.004621","article-title":"N-terminal Huntingtin (Htt) phosphorylation is a molecular switch regulating Htt aggregation, helical conformation, internalization, and nuclear targeting","volume":"293","author":"DeGuire","year":"2018","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.bbrc.2019.09.097_bib24","doi-asserted-by":"crossref","first-page":"2515","DOI":"10.1093\/hmg\/10.22.2515","article-title":"The HD mutation causes progressive lethal neurological disease in mice expressing reduced levels of huntingtin","volume":"10","author":"Auerbach","year":"2001","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.bbrc.2019.09.097_bib25","doi-asserted-by":"crossref","first-page":"5070","DOI":"10.1038\/s41598-017-05336-7","article-title":"Polyglutamine expansion affects huntingtin conformation in multiple Huntington\u2019s disease models","volume":"7","author":"Daldin","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bbrc.2019.09.097_bib26","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0112262","article-title":"Polyglutamine- and temperature-dependent conformational rigidity in mutant huntingtin revealed by immunoassays and circular dichroism spectroscopy","volume":"9","author":"Fodale","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.bbrc.2019.09.097_bib27","doi-asserted-by":"crossref","first-page":"2799","DOI":"10.1093\/hmg\/9.19.2799","article-title":"Dominant phenotypes produced by the HD mutation in STHdh(Q111) striatal cells","volume":"9","author":"Trettel","year":"2000","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.bbrc.2019.09.097_bib28","doi-asserted-by":"crossref","first-page":"16017","DOI":"10.1074\/jbc.M111.252577","article-title":"Identification of novel potentially toxic oligomers formed in\u00a0vitro from mammalian-derived expanded huntingtin exon-1 protein","volume":"287","author":"Nucifora","year":"2012","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bbrc.2019.09.097_bib29","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/j.neuron.2015.01.008","article-title":"N17 Modifies mutant Huntingtin nuclear pathogenesis and severity of disease in HD BAC transgenic mice","volume":"85","author":"Gu","year":"2015","journal-title":"Neuron"},{"key":"10.1016\/j.bbrc.2019.09.097_bib30","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1089\/hyb.2005.24.231","article-title":"Epitope mapping of monoclonal antibody 4C8 recognizing the protein huntingtin","volume":"24","author":"Cong","year":"2005","journal-title":"Hybridoma"}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X19318273?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X19318273?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T11:42:07Z","timestamp":1757763727000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X19318273"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":30,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["S0006291X19318273"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2019.09.097","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Ultrasensitive quantitative measurement of huntingtin phosphorylation at residue S13","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2019.09.097","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}