{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T16:44:11Z","timestamp":1786985051892,"version":"build-2736575974"},"reference-count":32,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100014472","name":"Scientific Research Foundation of Hunan Provincial Education Department","doi-asserted-by":"publisher","award":["19B240"],"award-info":[{"award-number":["19B240"]}],"id":[{"id":"10.13039\/100014472","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014472","name":"Scientific Research Foundation of Hunan Provincial Education Department","doi-asserted-by":"publisher","award":["19C1159"],"award-info":[{"award-number":["19C1159"]}],"id":[{"id":"10.13039\/100014472","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31770832"],"award-info":[{"award-number":["31770832"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31800655"],"award-info":[{"award-number":["31800655"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31900906"],"award-info":[{"award-number":["31900906"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of\u00a0Hunan Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2020,12]]},"DOI":"10.1016\/j.bbrc.2020.09.103","type":"journal-article","created":{"date-parts":[[2020,9,29]],"date-time":"2020-09-29T05:58:09Z","timestamp":1601359089000},"page":"958-964","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"title":["Recombinant PaurTx-3, a spider toxin, inhibits sodium channels and decreases membrane excitability in DRG neurons"],"prefix":"10.1016","volume":"533","author":[{"given":"Minzhi","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuijiao","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Si","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8491-0201","authenticated-orcid":false,"given":"Xi","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunxiao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhonghua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2020.09.103_bib1","doi-asserted-by":"crossref","first-page":"2577","DOI":"10.1113\/jphysiol.2011.224204","article-title":"Voltage-gated sodium channels at 60: structure, function and pathophysiology","volume":"590","author":"Catterall","year":"2012","journal-title":"J.\u00a0Physiol."},{"key":"10.1016\/j.bbrc.2020.09.103_bib2","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1146\/annurev.physiol.63.1.871","article-title":"Resurgence of sodium channel research","volume":"63","author":"Goldin","year":"2001","journal-title":"Annu. Rev. Physiol."},{"key":"10.1016\/j.bbrc.2020.09.103_bib3","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1007\/s13238-017-0372-z","article-title":"Structure-based assessment of disease-related mutations in human voltage-gated sodium channels","volume":"8","author":"Huang","year":"2017","journal-title":"Protein Cell"},{"key":"10.1016\/j.bbrc.2020.09.103_bib4","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/j.bbrc.2019.10.142","article-title":"Predicting novel disease mutations in the cardiac sodium channel","volume":"521","author":"Tarnovskaya","year":"2020","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2020.09.103_bib5","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/j.tins.2007.08.004","article-title":"From genes to pain: Na v 1.7 and human pain disorders","volume":"30","author":"Dib-Hajj","year":"2007","journal-title":"Trends Neurosci."},{"key":"10.1016\/j.bbrc.2020.09.103_bib6","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1517\/14728222.2016.1162295","article-title":"Nav1.7 and other voltage-gated sodium channels as drug targets for pain relief","volume":"20","author":"Emery","year":"2016","journal-title":"Expert Opin. Ther. Targets"},{"key":"10.1016\/j.bbrc.2020.09.103_bib7","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.toxicon.2012.04.337","article-title":"Spider-venom peptides that target voltage-gated sodium channels: pharmacological tools and potential therapeutic leads","volume":"60","author":"Klint","year":"2012","journal-title":"Toxicon"},{"key":"10.1016\/j.bbrc.2020.09.103_bib8","doi-asserted-by":"crossref","first-page":"114096","DOI":"10.1016\/j.bcp.2020.114096","article-title":"Animal toxins - nature\u2019s evolutionary-refined toolkit for basic research and drug discovery","author":"Herzig","year":"2020","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.bbrc.2020.09.103_bib9","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1586\/epr.09.45","article-title":"Venomics as a drug discovery platform","volume":"6","author":"Escoubas","year":"2009","journal-title":"Expert Rev. Proteomics"},{"key":"10.1016\/j.bbrc.2020.09.103_bib10","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1517\/14712598.2011.621940","article-title":"Venoms as a platform for human drugs: translating toxins into therapeutics","volume":"11","author":"King","year":"2011","journal-title":"Expet Opin. Biol. Ther."},{"key":"10.1016\/j.bbrc.2020.09.103_bib11","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1517\/17460441.2.6.823","article-title":"Spider peptide toxins as leads for drug development","volume":"2","author":"Escoubas","year":"2007","journal-title":"Expet Opin. Drug Discov."},{"key":"10.1016\/j.bbrc.2020.09.103_bib12","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.toxicon.2006.09.022","article-title":"Voltage-gated ion channels and gating modifier toxins","volume":"49","author":"Catterall","year":"2007","journal-title":"Toxicon"},{"key":"10.1016\/j.bbrc.2020.09.103_bib13","doi-asserted-by":"crossref","DOI":"10.3390\/toxins9080248","article-title":"Lengths of the C-terminus and interconnecting loops impact stability of spider-derived gating modifier toxins","volume":"9","author":"Agwa","year":"2017","journal-title":"Toxins (Basel)"},{"key":"10.1016\/j.bbrc.2020.09.103_bib14","doi-asserted-by":"crossref","first-page":"4366","DOI":"10.3390\/toxins7104366","article-title":"The cystine knot is responsible for the exceptional stability of the insecticidal spider toxin omega-hexatoxin-hv1a","volume":"7","author":"Herzig","year":"2015","journal-title":"Toxins (Basel)"},{"key":"10.1016\/j.bbrc.2020.09.103_bib15","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/978-1-4939-6887-9_10","article-title":"A\u00a0strategy for production of correctly folded disulfide-rich peptides in the periplasm of E. coli","volume":"1586","author":"Saez","year":"2017","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.bbrc.2020.09.103_bib16","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s12934-016-0618-0","article-title":"Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli","volume":"16","author":"Sequeira","year":"2017","journal-title":"Microb. Cell Factories"},{"key":"10.1016\/j.bbrc.2020.09.103_bib17","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1124\/mol.105.015941","article-title":"Four novel tarantula toxins as selective modulators of voltage-gated sodium channel subtypes","volume":"69","author":"Bosmans","year":"2006","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.bbrc.2020.09.103_bib18","doi-asserted-by":"crossref","first-page":"9041","DOI":"10.1074\/jbc.RA118.002553","article-title":"Gating modifier toxins isolated from spider venom: modulation of voltage-gated sodium channels and the role of lipid membranes","volume":"293","author":"Agwa","year":"2018","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bbrc.2020.09.103_bib19","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1124\/mol.110.066332","article-title":"The tarantula toxins ProTx-II and huwentoxin-IV differentially interact with human Nav1.7 voltage sensors to inhibit channel activation and inactivation","volume":"78","author":"Xiao","year":"2010","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.bbrc.2020.09.103_bib20","doi-asserted-by":"crossref","first-page":"20392","DOI":"10.1074\/jbc.M112.426627","article-title":"Structure and function of hainantoxin-III, a selective antagonist of neuronal tetrodotoxin-sensitive voltage-gated sodium channels isolated from the Chinese bird spider Ornithoctonus hainana","volume":"288","author":"Liu","year":"2013","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bbrc.2020.09.103_bib21","doi-asserted-by":"crossref","first-page":"40883","DOI":"10.1038\/srep40883","article-title":"Pharmacological characterisation of the highly NaV1.7 selective spider venom peptide Pn3a","volume":"7","author":"Deuis","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bbrc.2020.09.103_bib22","doi-asserted-by":"crossref","first-page":"27300","DOI":"10.1074\/jbc.M708447200","article-title":"Tarantula huwentoxin-IV inhibits neuronal sodium channels by binding to receptor site 4 and trapping the domain ii voltage sensor in the closed configuration","volume":"283","author":"Xiao","year":"2008","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bbrc.2020.09.103_bib23","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.peptides.2014.09.005","article-title":"Mapping the interaction site for the tarantula toxin hainantoxin-IV (beta-TRTX-Hn2a) in the voltage sensor module of domain II of voltage-gated sodium channels","volume":"68","author":"Cai","year":"2015","journal-title":"Peptides"},{"key":"10.1016\/j.bbrc.2020.09.103_bib24","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.jmb.2014.07.027","article-title":"From foe to friend: using animal toxins to investigate ion channel function","volume":"427","author":"Kalia","year":"2015","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.bbrc.2020.09.103_bib25","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1152\/physrev.00052.2017","article-title":"The role of voltage-gated sodium channels in pain signaling","volume":"99","author":"Bennett","year":"2019","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.bbrc.2020.09.103_bib26","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.3389\/fphar.2018.01000","article-title":"The NaV1.7 channel subtype as an antinociceptive target for spider toxins in adult dorsal root ganglia neurons","volume":"9","author":"Goncalves","year":"2018","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.bbrc.2020.09.103_bib27","doi-asserted-by":"crossref","DOI":"10.3390\/toxins12090529","article-title":"Two novel peptide toxins from the spider cyriopagopus longipes inhibit tetrodotoxin-sensitive sodium channels","volume":"12","author":"Zhang","year":"2020","journal-title":"Toxins (Basel)"},{"key":"10.1016\/j.bbrc.2020.09.103_bib28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1113\/jphysiol.2006.121483","article-title":"Multiple sodium channels and their roles in electrogenesis within dorsal root ganglion neurons","volume":"579","author":"Rush","year":"2007","journal-title":"J.\u00a0Physiol."},{"key":"10.1016\/j.bbrc.2020.09.103_bib29","doi-asserted-by":"crossref","first-page":"2293","DOI":"10.1038\/s41467-020-16210-y","article-title":"Spider venom-derived peptide induces hyperalgesia in Nav1.7 knockout mice by activating Nav1.9 channels","volume":"11","author":"Zhou","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bbrc.2020.09.103_bib30","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1016\/j.neuron.2019.01.047","article-title":"Defining the functional role of NaV1.7 in human nociception","volume":"101","author":"McDermott","year":"2019","journal-title":"Neuron"},{"key":"10.1016\/j.bbrc.2020.09.103_bib31","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1038\/nature05413","article-title":"An SCN9A channelopathy causes congenital inability to experience pain","volume":"444","author":"Cox","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.bbrc.2020.09.103_bib32","doi-asserted-by":"crossref","first-page":"10180","DOI":"10.1523\/JNEUROSCI.1049-18.2018","article-title":"Insensitivity to pain upon adult-onset deletion of Nav1.7 or its blockade with selective inhibitors","volume":"38","author":"Shields","year":"2018","journal-title":"J.\u00a0Neurosci."}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X20318556?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X20318556?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T14:39:02Z","timestamp":1762094342000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X20318556"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12]]},"references-count":32,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["S0006291X20318556"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2020.09.103","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2020,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Recombinant PaurTx-3, a spider toxin, inhibits sodium channels and decreases membrane excitability in DRG neurons","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2020.09.103","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}