{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:15:17Z","timestamp":1790615717811,"version":"4.1.0"},"reference-count":28,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003443","name":"Ministry of Education and Science of the Russian Federation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003443","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["16-14-10199"],"award-info":[{"award-number":["16-14-10199"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2021,1]]},"DOI":"10.1016\/j.bbrc.2020.11.103","type":"journal-article","created":{"date-parts":[[2020,12,8]],"date-time":"2020-12-08T14:02:00Z","timestamp":1607436120000},"page":"8-13","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"special_numbering":"C","title":["Effects of myopathy-causing mutations R91P and R245G in the TPM3 gene on structural and functional properties of slow skeletal muscle tropomyosin"],"prefix":"10.1016","volume":"534","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4326-5634","authenticated-orcid":false,"given":"Anastasiia D.","family":"Gonchar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2976-2108","authenticated-orcid":false,"given":"Galina V.","family":"Kopylova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia M.","family":"Kochurova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina Y.","family":"Berg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniil V.","family":"Shchepkin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0238-4920","authenticated-orcid":false,"given":"Natalia A.","family":"Koubasova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey K.","family":"Tsaturyan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5085-0327","authenticated-orcid":false,"given":"Sergey Y.","family":"Kleymenov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander M.","family":"Matyushenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitrii I.","family":"Levitsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2020.11.103_bib1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1023\/A:1010303732441","article-title":"Vertebrate tropomyosin: distribution, properties and function","volume":"22","author":"Perry","year":"2001","journal-title":"J.\u00a0Muscle Res. Cell Motil."},{"key":"10.1016\/j.bbrc.2020.11.103_bib2","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.1134\/S0006297911130098","article-title":"Tropomyosin: double helix from the protein world","volume":"76","author":"Nevzorov","year":"2011","journal-title":"Biochemistry (Mosc.)"},{"key":"10.1016\/j.bbrc.2020.11.103_bib3","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/S0006-3495(93)81110-X","article-title":"Regulation of the interaction between actin and myosin subfragment-1: evidence for three states of the thin filament","volume":"65","author":"McKillop","year":"1993","journal-title":"Biophys. J."},{"key":"10.1016\/j.bbrc.2020.11.103_bib4","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s10974-014-9389-6","article-title":"A\u00a0systematic nomenclature for mammalian tropomyosin isoforms","volume":"36","author":"Geeves","year":"2015","journal-title":"J.\u00a0Muscle Res. Cell Motil."},{"key":"10.1016\/j.bbrc.2020.11.103_bib5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bpc.2018.09.002","article-title":"Thermal unfolding of homodimers and heterodimers of different skeletal-muscle isoforms of tropomyosin","volume":"243","author":"Matyushenko","year":"2018","journal-title":"Biophys. Chem."},{"key":"10.1016\/j.bbrc.2020.11.103_bib6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.biochi.2020.03.013","article-title":"Unique functional properties of slow skeletal muscle tropomyosin","volume":"174","author":"Matyushenko","year":"2020","journal-title":"Biochimie"},{"key":"10.1016\/j.bbrc.2020.11.103_bib7","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1002\/humu.22554","article-title":"Mutation update and genotype\u2013phenotype correlations of novel and previously described mutations in TPM2 and TPM3 causing congenital myopathies","volume":"35","author":"Marttila","year":"2014","journal-title":"Hum. Mutat."},{"key":"10.1016\/j.bbrc.2020.11.103_bib8","doi-asserted-by":"crossref","first-page":"6278","DOI":"10.1093\/hmg\/ddv334","article-title":"Muscle weakness in TPM3-myopathy is due to reduced Ca2+-sensitivity and impaired acto-myosin cross-bridge cycling in slow fibres","volume":"24","author":"Yuen","year":"2015","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.bbrc.2020.11.103_bib9","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/s10974-019-09532-y","article-title":"Thin filament dysfunctions caused by mutations in tropomyosin Tpm3.12 and Tpm1.1","volume":"41","author":"Moraczewska","year":"2019","journal-title":"J.\u00a0Muscle Res. Cell Motil."},{"issue":"Suppl.\u00a01","key":"10.1016\/j.bbrc.2020.11.103_bib10","doi-asserted-by":"crossref","first-page":"S20","DOI":"10.1134\/S0006297920140023","article-title":"Molecular mechanisms of pathologies of skeletal and cardiac muscles caused by point mutations in the tropomyosin genes","volume":"85","author":"Matyushenko","year":"2020","journal-title":"Biochemistry (Mosc.)"},{"key":"10.1016\/j.bbrc.2020.11.103_bib11","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1002\/ana.21308","article-title":"Mutations in TPM3 are a common cause of congenital fiber type disproportion","volume":"63","author":"Clarke","year":"2008","journal-title":"Ann. Neurol."},{"key":"10.1016\/j.bbrc.2020.11.103_bib12","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1002\/humu.21157","article-title":"Mutations of tropomyosin 3 (TPM3) are common and associated with type 1 myofiber hypotrophy in congenital fiber type disproportion","volume":"31","author":"Lawlor","year":"2010","journal-title":"Hum. Mutat."},{"key":"10.1016\/j.bbrc.2020.11.103_bib13","doi-asserted-by":"crossref","first-page":"1562","DOI":"10.1016\/j.bbadis.2012.06.009","article-title":"Functional effects of congenital myopathy-related mutations in gamma-tropomyosin gene","volume":"1822","author":"Robaszkiewicz","year":"2012","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.bbrc.2020.11.103_bib14","doi-asserted-by":"crossref","first-page":"4978","DOI":"10.1093\/hmg\/ddt345","article-title":"Mutations in repeating structural motifs of tropomyosin cause gain of function in skeletal muscle myopathy patients","volume":"22","author":"Marston","year":"2013","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.bbrc.2020.11.103_bib15","doi-asserted-by":"crossref","first-page":"3975","DOI":"10.3390\/ijms19123975","article-title":"The primary causes of muscle dysfunction associated with the point mutations in Tpm3.12; conformational analysis of mutant proteins as a tool for classification of myopathies","volume":"19","author":"Borovikov","year":"2018","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.bbrc.2020.11.103_bib16","doi-asserted-by":"crossref","first-page":"10461","DOI":"10.1016\/S0021-9258(17)34082-6","article-title":"Functional \u03b1-tropomyosin produced in Escherichia coli. A dipeptide extension can substitute the amino-terminal acetyl group","volume":"269","author":"Monteiro","year":"1994","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.bbrc.2020.11.103_bib17","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/0076-6879(82)85020-9","article-title":"Purification of muscle actin","volume":"85 Pt B","author":"Pardee","year":"1982","journal-title":"Methods Enzymol."},{"issue":"Pt B","key":"10.1016\/j.bbrc.2020.11.103_bib18","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0076-6879(82)85009-X","article-title":"Preparation of myosin and its subfragments from rabbit skeletal muscle","volume":"85","author":"Margossian","year":"1982","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.bbrc.2020.11.103_bib19","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/0076-6879(82)85024-6","article-title":"Preparation of troponin and its subunits","volume":"85 Pt B","author":"Potter","year":"1982","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.bbrc.2020.11.103_bib20","doi-asserted-by":"crossref","first-page":"13507","DOI":"10.1096\/fj.202001318R","article-title":"Mechanisms of disturbance of the contractile function of slow skeletal muscles induced by myopathic mutations in the tropomyosin TPM3 gene","volume":"34","author":"Matyushenko","year":"2020","journal-title":"FASEB J."},{"key":"10.1016\/j.bbrc.2020.11.103_bib21","doi-asserted-by":"crossref","first-page":"2004","DOI":"10.1111\/febs.12756","article-title":"Structural and functional effects of two stabilizing substitutions, D137L and G126R, in the middle part of \u03b1-tropomyosin molecule","volume":"281","author":"Matyushenko","year":"2014","journal-title":"FEBS J."},{"key":"10.1016\/j.bbrc.2020.11.103_bib22","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1111\/febs.14369","article-title":"Functional role of the core gap in the middle part of tropomyosin","volume":"285","author":"Matyushenko","year":"2018","journal-title":"FEBS J."},{"key":"10.1016\/j.bbrc.2020.11.103_bib23","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1007\/s10974-017-9472-x","article-title":"Cooperativity of myosin interaction with thin filaments is enhanced by stabilizing substitutions in tropomyosin","volume":"38","author":"Shchepkin","year":"2017","journal-title":"J.\u00a0Muscle Res. Cell Motil."},{"key":"10.1016\/j.bbrc.2020.11.103_bib24","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.softx.2015.06.001","article-title":"GROMACS: high performance molecular simulations through multi-level parallelism from laptops to supercomputers","volume":"1\u20132","author":"Abraham","year":"2015","journal-title":"Software"},{"key":"10.1016\/j.bbrc.2020.11.103_bib25","article-title":"Tropomyosin pseudo-phosphorylation can rescue the effects of cardiomyopathy-associated mutation","author":"Nefedova","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.bbrc.2020.11.103_bib26","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera \u2013Visualization system for exploratory research and analysis","volume":"25","author":"Pettersen","year":"2004","journal-title":"J.\u00a0Comput. Chem."},{"key":"10.1016\/j.bbrc.2020.11.103_bib27","doi-asserted-by":"crossref","first-page":"3376","DOI":"10.3390\/ijms19113376","article-title":"Effects of an interchain disulfide bond on tropomyosin structure: a molecular dynamics study","volume":"19","author":"Koubassova","year":"2018","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.bbrc.2020.11.103_bib28","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1021\/acs.biochem.6b00994","article-title":"Structural and functional effects of cardiomyopathy-causing mutations in the troponin T-binding region of cardiac tropomyosin","volume":"56","author":"Matyushenko","year":"2017","journal-title":"Biochemistry"}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X2032146X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X2032146X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T18:04:01Z","timestamp":1777399441000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X2032146X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":28,"alternative-id":["S0006291X2032146X"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2020.11.103","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Effects of myopathy-causing mutations R91P and R245G in the TPM3 gene on structural and functional properties of slow skeletal muscle tropomyosin","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2020.11.103","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}