{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T18:56:39Z","timestamp":1758048999096,"version":"3.44.0"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T00:00:00Z","timestamp":1619654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010663","name":"European Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2021,6]]},"DOI":"10.1016\/j.bbrc.2021.04.071","type":"journal-article","created":{"date-parts":[[2021,5,5]],"date-time":"2021-05-05T06:48:46Z","timestamp":1620197326000},"page":"245-251","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["The effect of TGF\u03b2RI inhibition on extracellular matrix structure and stiffness in hypertrophic scar-specific fibroblast-derived matrix models"],"prefix":"10.1016","volume":"559","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4168-6443","authenticated-orcid":false,"given":"Rajiv S.","family":"Raktoe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3785-9217","authenticated-orcid":false,"given":"Ludo","family":"van Haasterecht","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9080-0324","authenticated-orcid":false,"given":"Nelda","family":"Antonovaite","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3616-2701","authenticated-orcid":false,"given":"Luca","family":"Bartolini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7019-6673","authenticated-orcid":false,"given":"Remco","family":"van Doorn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3461-8848","authenticated-orcid":false,"given":"Paul P.M.","family":"van Zuijlen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5360-6987","authenticated-orcid":false,"given":"Marie Louise","family":"Groot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdoelwaheb","family":"EL Ghalbzouri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.bbrc.2021.04.071_bib1","doi-asserted-by":"crossref","first-page":"R188","DOI":"10.1186\/cc9300","article-title":"Severe burn injury in europe: a systematic review of the incidence, etiology, morbidity, and mortality","volume":"14","author":"Brusselaers","year":"2010","journal-title":"Crit. Care"},{"issue":"10","key":"10.1016\/j.bbrc.2021.04.071_bib2","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1007\/s00403-006-0651-7","article-title":"Quality of life of patients with keloid and hypertrophic scarring","volume":"297","author":"Bock","year":"2006","journal-title":"Arch. Dermatol. Res."},{"issue":"3","key":"10.1016\/j.bbrc.2021.04.071_bib3","first-page":"701","article-title":"Hypertrophic scars, Keloids and Contractures. The cellular and molecular basis for therapy","volume":"77","author":"Tredget","year":"1997","journal-title":"Surg. Clin."},{"issue":"2","key":"10.1016\/j.bbrc.2021.04.071_bib4","first-page":"485","article-title":"Enhanced expression of transforming growth factor-beta type I and type II receptors in wound granulation tissue and hypertrophic scar","volume":"152","author":"Schmid","year":"1998","journal-title":"Am. J. Pathol."},{"issue":"3","key":"10.1016\/j.bbrc.2021.04.071_bib5","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1038\/35043051","article-title":"How cells read TGF-beta signals","volume":"1","author":"Massagu\u00e9","year":"2000","journal-title":"Nat. Rev. Mol. Cell Biol."},{"issue":"1","key":"10.1016\/j.bbrc.2021.04.071_bib6","first-page":"18","article-title":"The role of the TGF-\u03b2 family in wound healing, burns and scarring: a review","volume":"2","author":"Penn","year":"2012","journal-title":"Int J Burns Trauma"},{"issue":"999","key":"10.1016\/j.bbrc.2021.04.071_bib7","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1136\/pgmj.2008.069831","article-title":"Transforming growth factor beta1 signalling, wound healing and repair: a multifunctional cytokine with clinical implications for wound repair, a delicate balance","volume":"85","author":"Klass","year":"2009","journal-title":"Postgrad. Med."},{"issue":"2","key":"10.1016\/j.bbrc.2021.04.071_bib8","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/S1471-4892(03)00002-X","article-title":"ALK5 inhibition in renal disease","volume":"3","author":"Laping","year":"2003","journal-title":"Curr. Opin. Pharmacol."},{"issue":"1","key":"10.1016\/j.bbrc.2021.04.071_bib9","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.cytogfr.2005.09.005","article-title":"Role of anti-TGF-\u03b2 antibodies in the treatment of renal injury","volume":"17","author":"Gagliardini","year":"2006","journal-title":"Cytokine Growth Factor Rev."},{"issue":"6","key":"10.1016\/j.bbrc.2021.04.071_bib10","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1097\/BOR.0b013e32830e48e8","article-title":"Antitransforming growth factor-beta therapy in fibrosis: recent progress and implications for systemic sclerosis","volume":"20","author":"Varga","year":"2008","journal-title":"Curr. Opin. Rheumatol."},{"issue":"1","key":"10.1016\/j.bbrc.2021.04.071_bib11","doi-asserted-by":"crossref","first-page":"123","DOI":"10.2174\/1566523044578004","article-title":"TGF-b: a fibrotic factor in wound scarring and a potential target for anti- scarring gene therapy","volume":"4","author":"Liu","year":"2004","journal-title":"Curr. Gene Ther."},{"key":"10.1016\/j.bbrc.2021.04.071_bib12","doi-asserted-by":"crossref","first-page":"461","DOI":"10.3389\/fphar.2017.00461","article-title":"Targeting TGF-\u03b2 mediated SMAD signaling for the prevention of fibrosis","volume":"8","author":"Walton","year":"2017","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.bbrc.2021.04.071_bib13","doi-asserted-by":"crossref","first-page":"e156","DOI":"10.1038\/mtna.2014.7","article-title":"Targeting TGF-\u03b2 signaling by antisense oligonucleotide-mediated knockdown of TGF-\u03b2 type I receptor","volume":"3","author":"Kemaladewi","year":"2014","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"10.1016\/j.bbrc.2021.04.071_bib14","doi-asserted-by":"crossref","first-page":"e142","DOI":"10.1038\/mtna.2013.69","article-title":"Novel ex\u00a0vivo culture method for the study of Dupuytren's disease: effects of TGF\u03b2 type 1 receptor modulation by antisense oligonucleotides","volume":"3","author":"Karkampouna","year":"2014","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"10.1016\/j.bbrc.2021.04.071_bib15","first-page":"1","article-title":"Exon skipping of TGF\u03b2RI affects signalling and ECM expression in hypertrophic scar-derived fibroblasts","volume":"6","author":"Raktoe","year":"2020","journal-title":"Scars, Burn Heal."},{"issue":"1\u20132","key":"10.1016\/j.bbrc.2021.04.071_bib16","first-page":"109","article-title":"Galangin inhibits hypertrophic scar formation via ALK5\/Smad2\/3 signaling pathway","volume":"413","author":"Zhang","year":"2016","journal-title":"Mol. Cell. Biochem."},{"issue":"1","key":"10.1016\/j.bbrc.2021.04.071_bib17","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1111\/bjd.14108","article-title":"Baicalein attenuates hypertrophic scar formation via inhibition of the transforming growth factor-\u03b2\/Smad2\/3 signalling pathway","volume":"174","author":"Zhang","year":"2016","journal-title":"Br. J. Dermatol."},{"issue":"5","key":"10.1016\/j.bbrc.2021.04.071_bib18","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1111\/j.1524-475X.2009.00533.x","article-title":"Differences in collagen architecture between keloid, hypertrophic scar, normotrophic scar, and normal skin: an objective histopathological analysis","volume":"17","author":"Verhaegen","year":"2009","journal-title":"Wound Repair Regen."},{"issue":"1","key":"10.1016\/j.bbrc.2021.04.071_bib19","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1002\/ar.1091790111","article-title":"Collagen and dermal patterns in the hypertrophic scar","volume":"179","author":"Kischer","year":"1974","journal-title":"Anat. Rec."},{"issue":"1","key":"10.1016\/j.bbrc.2021.04.071_bib20","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/1523-1747.ep12274528","article-title":"Mechanical analysis of hypertrophic scar tissue: structural basis for apparent increased rigidity","volume":"84","author":"Dunn","year":"1985","journal-title":"J.\u00a0Invest. Dermatol."},{"issue":"6","key":"10.1016\/j.bbrc.2021.04.071_bib21","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/0305-4179(96)00038-1","article-title":"Mechanical properties of normal skin and hypertrophic scars","volume":"22","author":"Clark","year":"1996","journal-title":"Burns"},{"issue":"2","key":"10.1016\/j.bbrc.2021.04.071_bib22","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/0014-4827(89)90105-5","article-title":"Collagen processing, crosslinking, and fibril bundle assembly in matrix produced by fibroblasts in long-term cultures supplemented with ascorbic acid","volume":"181","author":"Grinnell","year":"1989","journal-title":"Exp. Cell Res."},{"issue":"1","key":"10.1016\/j.bbrc.2021.04.071_bib23","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.biomaterials.2008.09.002","article-title":"Replacement of animal-derived collagen matrix by human fibroblast-derived dermal matrix for human skin equivalent products","volume":"30","author":"El Ghalbzouri","year":"2009","journal-title":"Biomaterials"},{"issue":"1","key":"10.1016\/j.bbrc.2021.04.071_bib24","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1046\/j.1365-2133.1997.d01-1134.x","article-title":"Morphological and biochemical analyses on fibroblasts and self-produced collagens in a novel three-dimensional culture","volume":"136","author":"Ishikawa","year":"1997","journal-title":"Br. J. Dermatol."},{"issue":"13","key":"10.1016\/j.bbrc.2021.04.071_bib25","doi-asserted-by":"crossref","first-page":"2183","DOI":"10.1016\/j.biomaterials.2006.12.030","article-title":"Biomechanical and biochemical characteristics of a human fibroblast-produced and remodeled matrix","volume":"28","author":"Ahlfors","year":"2007","journal-title":"Biomaterials"},{"issue":"8","key":"10.1016\/j.bbrc.2021.04.071_bib26","doi-asserted-by":"crossref","DOI":"10.3390\/ijms18081772","article-title":"Imaging collagen in scar tissue: developments in second harmonic generation microscopy for biomedical applications","volume":"18","author":"Mosta\u00e7o-Guidolin","year":"2017","journal-title":"Int. J. Mol. Sci."},{"issue":"4","key":"10.1016\/j.bbrc.2021.04.071_bib27","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1063\/1.437677","article-title":"Second harmonic generation in collagen","volume":"70","author":"Roth","year":"1979","journal-title":"J.\u00a0Chem. Phys."},{"issue":"11","key":"10.1016\/j.bbrc.2021.04.071_bib28","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1038\/nbt899","article-title":"Nonlinear magic: multiphoton microscopy in the biosciences","volume":"21","author":"Zipfel","year":"2003","journal-title":"Nat. Biotechnol."},{"issue":"5","key":"10.1016\/j.bbrc.2021.04.071_bib29","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/S1367-5931(00)00241-6","article-title":"Multiphoton microscopy in biological research","volume":"5","author":"Williams","year":"2001","journal-title":"Curr. Opin. Chem. Biol."},{"issue":"6","key":"10.1016\/j.bbrc.2021.04.071_bib30","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1016\/j.burns.2011.03.013","article-title":"Second harmonic generation and multiphoton microscopic detection of collagen without the need for species specific antibodies","volume":"37","author":"Chen","year":"2011","journal-title":"Burns"},{"issue":"1","key":"10.1016\/j.bbrc.2021.04.071_bib31","doi-asserted-by":"crossref","first-page":"12517","DOI":"10.1038\/s41598-018-31035-y","article-title":"Regional variations in stiffness in live mouse brain tissue determined by depth-controlled indentation mapping","volume":"8","author":"Antonovaite","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bbrc.2021.04.071_bib32","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/j.actbio.2019.07.056","article-title":"Micro-indentation and optical coherence tomography for the mechanical characterization of embryos: experimental setup and measurements on fixed chicken embryos","volume":"97","author":"Marrese","year":"2019","journal-title":"Acta Biomater."},{"issue":"17","key":"10.1016\/j.bbrc.2021.04.071_bib33","doi-asserted-by":"crossref","first-page":"11014","DOI":"10.1073\/pnas.172368799","article-title":"Imaging cells and extracellular matrix in\u00a0vivo by using second-harmonic generation and two-photon excited fluorescence","volume":"99","author":"Zoumi","year":"2002","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"1","key":"10.1016\/j.bbrc.2021.04.071_bib34","doi-asserted-by":"crossref","first-page":"e1610","DOI":"10.1097\/GOX.0000000000001610","article-title":"Structural and mechanical comparison of human ear, alar, and septal cartilage","volume":"6","author":"Bos","year":"2018","journal-title":"Plast Reconstr Surg Glob Open"},{"key":"10.1016\/j.bbrc.2021.04.071_bib35","first-page":"36","article-title":"Image processing with ImageJ","volume":"11","author":"Abramoff","year":"2003","journal-title":"Biophot. Int."},{"issue":"6","key":"10.1016\/j.bbrc.2021.04.071_bib36","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1016\/j.bone.2010.08.023","article-title":"BoneJ: free and extensible bone image analysis in ImageJ","volume":"47","author":"Doube","year":"2010","journal-title":"Bone"},{"key":"10.1016\/j.bbrc.2021.04.071_bib37","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1007\/978-1-4939-7113-8_28","article-title":"Methods for quantifying fibrillar collagen alignment","volume":"1627","author":"Liu","year":"2017","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.bbrc.2021.04.071_bib38","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.actbio.2017.02.011","article-title":"Structural characterization and viscoelastic constitutive modeling of skin","volume":"53","author":"Sherman","year":"2017","journal-title":"Acta Biomater."},{"issue":"12","key":"10.1016\/j.bbrc.2021.04.071_bib39","doi-asserted-by":"crossref","first-page":"3066","DOI":"10.1039\/C6SM00300A","article-title":"Local dynamic mechanical analysis for heterogeneous soft matter using ferrule-top indentation","volume":"12","author":"van Hoorn","year":"2016","journal-title":"Soft Matter"},{"issue":"5","key":"10.1016\/j.bbrc.2021.04.071_bib40","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1007\/s10237-008-0139-9","article-title":"Spherical indentation of soft matter beyond the Hertzian regime: numerical and experimental validation of hyperelastic models","volume":"8","author":"Lin","year":"2009","journal-title":"Biomech. Model. Mechanobiol."}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X21006811?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X21006811?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T12:07:22Z","timestamp":1757765242000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X21006811"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6]]},"references-count":40,"alternative-id":["S0006291X21006811"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2021.04.071","relation":{},"ISSN":["0006-291X"],"issn-type":[{"type":"print","value":"0006-291X"}],"subject":[],"published":{"date-parts":[[2021,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The effect of TGF\u03b2RI inhibition on extracellular matrix structure and stiffness in hypertrophic scar-specific fibroblast-derived matrix models","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2021.04.071","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}