{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T01:58:03Z","timestamp":1792634283782,"version":"4.5.0"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003725","name":"NRF","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003710","name":"KHIDI","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2022,1]]},"DOI":"10.1016\/j.bbrc.2021.11.084","type":"journal-article","created":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T12:36:21Z","timestamp":1638189381000},"page":"42-48","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"special_numbering":"C","title":["Label-free imaging and evaluation of characteristic properties of asthma-derived eosinophils using optical diffraction tomography"],"prefix":"10.1016","volume":"587","author":[{"given":"Sang-Yeob","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Hyang","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeonhee","family":"Shin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5533-5938","authenticated-orcid":false,"given":"Tae-Keun","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji won","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Ju","family":"Pyo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.Ryang","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6578-3099","authenticated-orcid":false,"given":"Chan-Gi","family":"Pack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8767-2667","authenticated-orcid":false,"given":"You Sook","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2021.11.084_bib1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1002\/JLB.3MR1217-476R","article-title":"Contemporary understanding of the secretory granules in human eosinophils","volume":"104","author":"Melo","year":"2018","journal-title":"J.\u00a0Leukoc. Biol."},{"key":"10.1016\/j.bbrc.2021.11.084_bib2","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.anai.2018.02.031","article-title":"The eosinophil: for better or worse, in sickness and in health","volume":"121","author":"Bochner","year":"2018","journal-title":"Ann. Allergy Asthma Immunol."},{"key":"10.1016\/j.bbrc.2021.11.084_bib3","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1038\/nri.2017.95","article-title":"Functions of tissue-resident eosinophils","volume":"17","author":"Weller","year":"2017","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.bbrc.2021.11.084_bib4","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1111\/j.1365-2222.2005.02335.x","article-title":"Circulating eosinophils in asthma, allergic rhinitis, and atopic dermatitis lack morphological signs of degranulation","volume":"35","author":"Malm-Erjef\u00e4lt","year":"2005","journal-title":"Clin. Exp. Allergy"},{"key":"10.1016\/j.bbrc.2021.11.084_bib5","doi-asserted-by":"crossref","first-page":"1728","DOI":"10.1016\/j.jaci.2013.11.024","article-title":"Electron microscopy elucidates eosinophil degranulation patterns in patients with eosinophilic esophagitis","volume":"133","author":"Saffari","year":"2014","journal-title":"J.\u00a0Allergy Clin. Immunol."},{"key":"10.1016\/j.bbrc.2021.11.084_bib6","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1111\/all.12176","article-title":"Morphological changes in eosinophils are reliable markers of the severity of an acute asthma exacerbation in children","volume":"68","author":"Muniz-Junqueira","year":"2013","journal-title":"Allergy"},{"key":"10.1016\/j.bbrc.2021.11.084_bib7","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.3389\/fphys.2019.01261","article-title":"Atomic force microscopy is a potent technique to study eosinophil activation","volume":"10","author":"Eaton","year":"2019","journal-title":"Front. Physiol."},{"key":"10.1016\/j.bbrc.2021.11.084_bib8","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1111\/bjh.17026","article-title":"The role of eosinophil morphology in distinguishing between reactive eosinophilia and eosinophilia as a feature of a myeloid neoplasm","volume":"191","author":"Goasguen","year":"2020","journal-title":"Br. J. Haematol."},{"key":"10.1016\/j.bbrc.2021.11.084_bib9","first-page":"335","article-title":"Morphology and staning behavior of neutrophilic and eosinophilic granulocytes of the common marmoset","volume":"68","author":"Bleyer","year":"2016","journal-title":"Callithrix Jacchus"},{"key":"10.1016\/j.bbrc.2021.11.084_bib10","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1017\/S1431927610093864","article-title":"Contributions of electron microscopy to understand secretion of immune mediators by human eosinophils","volume":"16","author":"Melo","year":"2010","journal-title":"Microsc. Microanal."},{"key":"10.1016\/j.bbrc.2021.11.084_bib11","series-title":"Eosinophils in Health and Disease","first-page":"19","article-title":"Eosinophil structure and cell surface receptors","author":"Weller","year":"2013"},{"key":"10.1016\/j.bbrc.2021.11.084_bib12","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1182\/blood.V57.6.1099.1099","article-title":"Eosinophil autofluorescence and its use in isolation and analysis of human eosinophils using flow microfluorometry","volume":"57","author":"Weil","year":"1981","journal-title":"Blood"},{"key":"10.1016\/j.bbrc.2021.11.084_bib13","doi-asserted-by":"crossref","first-page":"14779","DOI":"10.1073\/pnas.2001404117","article-title":"Label-free hematology analysis using deep-ultraviolet microscopy","volume":"117","author":"Ojaghi","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.bbrc.2021.11.084_bib14","doi-asserted-by":"crossref","first-page":"5335","DOI":"10.1021\/ac3007363","article-title":"Toward a spectroscopic hemogram: Raman spectroscopic differentiation of the two most abundant leukocytes from peripheral blood","volume":"84","author":"Ramoji","year":"2012","journal-title":"Anal. Chem."},{"key":"10.1016\/j.bbrc.2021.11.084_bib15","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1111\/bjh.15971","article-title":"Raman imaging highlights biochemical heterogeneity of human eosinophils versus human eosinophilic leukaemia cell line","volume":"186","author":"Rygula","year":"2019","journal-title":"Br. J. Haematol."},{"key":"10.1016\/j.bbrc.2021.11.084_bib16","doi-asserted-by":"crossref","first-page":"13730","DOI":"10.1073\/pnas.0806100105","article-title":"Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum","volume":"105","author":"Park","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.bbrc.2021.11.084_bib17","article-title":"Optical diffraction tomography techniques for the study of cell pathophysiology","volume":"2","author":"Kim","year":"2016","journal-title":"J.\u00a0Biomed. Photon. & Eng."},{"key":"10.1016\/j.bbrc.2021.11.084_bib18","doi-asserted-by":"crossref","first-page":"699","DOI":"10.3390\/cells8070699","article-title":"Physicochemical properties of nucleoli in live cells analyzed by label-free optical diffraction tomography","volume":"8","author":"Kim","year":"2019","journal-title":"Cells"},{"key":"10.1016\/j.bbrc.2021.11.084_bib19","doi-asserted-by":"crossref","first-page":"3865","DOI":"10.1364\/BOE.6.003865","article-title":"Label-free characterization of white blood cells by measuring 3D refractive index maps","volume":"6","author":"Yoon","year":"2015","journal-title":"Biomed. Opt Express"},{"key":"10.1016\/j.bbrc.2021.11.084_bib20","doi-asserted-by":"crossref","first-page":"6654","DOI":"10.1038\/s41598-017-06311-y","article-title":"Identification of non-activated lymphocytes using three-dimensional refractive index tomography and machine learning","volume":"7","author":"Yoon","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bbrc.2021.11.084_bib21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s12276-018-0167-1","article-title":"Biological function of eosinophil extracellular traps in patients with severe eosinophilic asthma","volume":"50","author":"Choi","year":"2018","journal-title":"Exp. Mol. Med."},{"key":"10.1016\/j.bbrc.2021.11.084_bib22","doi-asserted-by":"crossref","first-page":"9192","DOI":"10.1038\/s41598-018-25886-8","article-title":"Measurements of three-dimensional refractive index tomography and membrane deformability of live erythrocytes from Pelophylax nigromaculatus","volume":"8","author":"Kim","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bbrc.2021.11.084_bib23","doi-asserted-by":"crossref","first-page":"4677","DOI":"10.1364\/OL.36.004677","article-title":"Real-time quantitative phase imaging with a spatial phase-shifting algorithm","volume":"36","author":"Debnath","year":"2011","journal-title":"Opt. Lett."},{"key":"10.1016\/j.bbrc.2021.11.084_bib24","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1189\/jlb.0212067","article-title":"Eosinophil crystalloid granules: structure, function, and beyond","volume":"92","author":"Muniz","year":"2012","journal-title":"J.\u00a0Leukoc. Biol."},{"key":"10.1016\/j.bbrc.2021.11.084_bib25","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1084\/jem.186.6.909","article-title":"Eosinophil lipid bodies: specific, inducible intracellular sites for enhanced eicosanoid formation","volume":"186","author":"Bozza","year":"1997","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.bbrc.2021.11.084_bib26","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1369\/0022155411404073","article-title":"Lipid bodies in inflammatory cells: structure, function, and current imaging techniques","volume":"59","author":"Melo","year":"2011","journal-title":"J.\u00a0Histochem. Cytochem."},{"key":"10.1016\/j.bbrc.2021.11.084_bib27","doi-asserted-by":"crossref","first-page":"36815","DOI":"10.1038\/srep36815","article-title":"Three-dimensional label-free imaging and quantification of lipid droplets in live hepatocytes","volume":"6","author":"Kim","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bbrc.2021.11.084_bib28","doi-asserted-by":"crossref","first-page":"496","DOI":"10.3389\/fimmu.2014.00496","article-title":"Eosinophil secretion of granule-derived cytokines","volume":"5","author":"Spencer","year":"2014","journal-title":"Front. Immunol."},{"key":"10.1016\/j.bbrc.2021.11.084_bib29","doi-asserted-by":"crossref","first-page":"259","DOI":"10.5858\/arpa.2011-0597-RA","article-title":"Diagnostic complexities of eosinophilia","volume":"137","author":"Montgomery","year":"2013","journal-title":"Arch. Pathol. Lab Med."}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X21016090?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X21016090?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T12:12:27Z","timestamp":1757765547000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X21016090"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1]]},"references-count":29,"alternative-id":["S0006291X21016090"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2021.11.084","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2022,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Label-free imaging and evaluation of characteristic properties of asthma-derived eosinophils using optical diffraction tomography","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2021.11.084","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}