{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T08:04:33Z","timestamp":1769933073977,"version":"3.49.0"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2022,7]]},"DOI":"10.1016\/j.bbrc.2022.04.104","type":"journal-article","created":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T21:38:23Z","timestamp":1650922703000},"page":"134-140","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["H55\u00a0N variation in citrate synthase leads to decrement in the enzyme activity and transport rate to mitochondria in HEI-OC1 cells"],"prefix":"10.1016","volume":"612","author":[{"given":"Zhen","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rongrong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingying","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengmeng","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Luan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1865-143X","authenticated-orcid":false,"given":"Wenjing","family":"Shang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xicheng","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengchan","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"10","key":"10.1016\/j.bbrc.2022.04.104_bib1","doi-asserted-by":"crossref","first-page":"1820","DOI":"10.2105\/AJPH.2016.303299","article-title":"Prevalence of hearing loss by severity in the United States","volume":"106","author":"Goman","year":"2016","journal-title":"Am. J. Publ. Health"},{"issue":"3","key":"10.1016\/j.bbrc.2022.04.104_bib2","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1001\/archinternmed.2011.1408","article-title":"Prevalence of hearing aid use among older adults in the United States","volume":"172","author":"Chien","year":"2012","journal-title":"Arch. Intern. Med."},{"key":"10.1016\/j.bbrc.2022.04.104_bib3","author":"World Health Organization"},{"issue":"3","key":"10.1016\/j.bbrc.2022.04.104_bib4","doi-asserted-by":"crossref","first-page":"126","DOI":"10.3342\/ceo.2010.3.3.126","article-title":"Comparison of cochlear morphology and apoptosis in mouse models of presbycusis","volume":"3","author":"Park","year":"2010","journal-title":"Clin. Exp. Otorhinolaryngol."},{"key":"10.1016\/j.bbrc.2022.04.104_bib5","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.heares.2015.05.006","article-title":"Attenuation of hearing loss in DBA\/2J mice by anti-apoptotic treatment","volume":"327","author":"Yang","year":"2015","journal-title":"Hear. Res."},{"issue":"4","key":"10.1016\/j.bbrc.2022.04.104_bib6","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0014807","article-title":"Fascin 2b is a component of stereocilia that lengthens actin-based protrusions","volume":"6","author":"Chou","year":"2011","journal-title":"PLoS One"},{"issue":"1\u20132","key":"10.1016\/j.bbrc.2022.04.104_bib7","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0378-5955(97)00155-X","article-title":"A\u00a0major gene affecting age-related hearing loss in C57BL\/6J mice","volume":"114","author":"Johnson","year":"1997","journal-title":"Hear. Res."},{"issue":"1","key":"10.1016\/j.bbrc.2022.04.104_bib8","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/0378-5955(91)90084-M","article-title":"Hereditary deafness occurring in cd\/1 mice","volume":"57","author":"Shone","year":"1991","journal-title":"Hear. Res."},{"issue":"2","key":"10.1016\/j.bbrc.2022.04.104_bib9","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1038\/84831","article-title":"A\u00a0nuclear-mitochondrial DNA interaction affecting hearing impairment in mice","volume":"27","author":"Johnson","year":"2001","journal-title":"Nat. Genet."},{"issue":"10","key":"10.1016\/j.bbrc.2022.04.104_bib10","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1016\/j.neurobiolaging.2007.12.011","article-title":"A\u00a0locus on distal chromosome 10 (ahl4) affecting age-related hearing loss in A\/J mice","volume":"30","author":"Zheng","year":"2009","journal-title":"Neurobiol. Aging"},{"issue":"8","key":"10.1016\/j.bbrc.2022.04.104_bib11","doi-asserted-by":"crossref","first-page":"1720","DOI":"10.1016\/j.neurobiolaging.2011.05.009","article-title":"Association of a citrate synthase missense mutation with age-related hearing loss in A\/J mice","volume":"33","author":"Johnson","year":"2012","journal-title":"Neurobiol. Aging"},{"issue":"2","key":"10.1016\/j.bbrc.2022.04.104_bib12","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1152\/physiolgenomics.00066.2010","article-title":"H55N polymor-phism as a likely cause of variation in citrate synthase activity of mouse skeletal muscle","volume":"42A","author":"Ratkevicius","year":"2010","journal-title":"Physiol Geno-mics."},{"key":"10.1016\/j.bbrc.2022.04.104_bib13","doi-asserted-by":"crossref","DOI":"10.1155\/2021\/9957908","article-title":"Citrate synthase and OGDH as potential biomarkers of atherosclerosis under chronic stress","volume":"2021","author":"Meng","year":"2021","journal-title":"Oxid. Med. Cell. Longev."},{"key":"10.1016\/j.bbrc.2022.04.104_bib14","doi-asserted-by":"crossref","DOI":"10.1155\/2015\/617207","article-title":"Reactive oxygen species, apoptosis, and mitochondrial dysfunction in hearing loss","volume":"2015","author":"Kamogashira","year":"2015","journal-title":"BioMed Res. Int."},{"issue":"1\u20132","key":"10.1016\/j.bbrc.2022.04.104_bib15","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.heares.2005.06.009","article-title":"Cu\/Zn superoxide dismutase and age-related hearing loss","volume":"209","author":"Keithley","year":"2005","journal-title":"Hear. Res."},{"issue":"1","key":"10.1016\/j.bbrc.2022.04.104_bib16","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1002\/(SICI)1096-9861(19991011)413:1<101::AID-CNE7>3.0.CO;2-L","article-title":"Cu\/Zn SOD deficiency potentiates hearing loss and cochlear pathology in aged 129,CD-1 mice","volume":"413","author":"Mcfadden","year":"1999","journal-title":"J.\u00a0Comp. Neurol."},{"issue":"3","key":"10.1016\/j.bbrc.2022.04.104_bib17","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/s101620010043","article-title":"Targeted mutation of the gene for cellular glutathione peroxidase (Gpx1) increases noise-induced hearing loss in mice","volume":"1","author":"Ohlemiller","year":"2000","journal-title":"J.\u00a0Assoc. Res. Otolaryngol."},{"issue":"2","key":"10.1016\/j.bbrc.2022.04.104_bib18","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/j.bbrc.2017.02.067","article-title":"Reduced expression of citrate synthase leads to excessive superoxide formation and cell apoptosis","volume":"485","author":"Cai","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2022.04.104_bib19","doi-asserted-by":"crossref","first-page":"108402","DOI":"10.1016\/j.abb.2020.108402","article-title":"ALA protects against ERS-mediated apoptosis in a cochlear cell model with low citrate synthase expression","volume":"688","author":"Xu","year":"2020","journal-title":"Arch. Biochem. Biophys."},{"issue":"41","key":"10.1016\/j.bbrc.2022.04.104_bib20","doi-asserted-by":"crossref","first-page":"13278","DOI":"10.1021\/bi00041a003","article-title":"Catalytic strategy of citrate synthase: subunit interactions revealed as a consequence of a single amino acid change in the oxaloacetate binding site","volume":"34","author":"Kurz","year":"1995","journal-title":"Biochemistry"},{"issue":"11","key":"10.1016\/j.bbrc.2022.04.104_bib21","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0185789","article-title":"H55N polymorphism is associated with low citrate synthase activity which regulates lipid metabolism in mouse muscle cells","volume":"12","author":"Gabriel","year":"2017","journal-title":"PLoS One"},{"issue":"19\u201320","key":"10.1016\/j.bbrc.2022.04.104_bib22","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1101\/gad.316547.118","article-title":"Pathways to balance mitochondrial translation and protein import","volume":"32","author":"Priesnitz","year":"2018","journal-title":"Genes Dev."},{"key":"10.1016\/j.bbrc.2022.04.104_bib23","article-title":"DeepMind\u2019s AI for protein structure is coming to the masses","author":"Callaway","year":"2021","journal-title":"Nature"},{"issue":"7873","key":"10.1016\/j.bbrc.2022.04.104_bib24","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"issue":"6557","key":"10.1016\/j.bbrc.2022.04.104_bib25","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1126\/science.abj8754","article-title":"Accurate prediction of protein structures and interactions using a three-track neural network","volume":"373","author":"Baek","year":"2021","journal-title":"Science"},{"issue":"1","key":"10.1016\/j.bbrc.2022.04.104_bib26","doi-asserted-by":"crossref","DOI":"10.1177\/1759091415573985","article-title":"Caspase-mediated apoptosis in the cochleae contributes to the early onset of hearing loss in A\/J mice","volume":"7","author":"Han","year":"2015","journal-title":"ASN Neuro"},{"key":"10.1016\/j.bbrc.2022.04.104_bib27","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.mib.2014.09.008","article-title":"Endosymbiotic theory for organelle origins","volume":"22","author":"Zimorski","year":"2014","journal-title":"Curr. Opin. Microbiol."},{"issue":"19","key":"10.1016\/j.bbrc.2022.04.104_bib28","doi-asserted-by":"crossref","first-page":"R911","DOI":"10.1016\/j.cub.2015.07.055","article-title":"Endosymbiosis and eukaryotic cell evolution","volume":"25","author":"Archibald","year":"2015","journal-title":"Curr. Biol."},{"issue":"1","key":"10.1016\/j.bbrc.2022.04.104_bib29","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0092-8674(02)01250-3","article-title":"Molecular chaperones Hsp90 and Hsp70 deliver preproteins to the mitochondrial import receptor Tom70","volume":"112","author":"Young","year":"2003","journal-title":"Cell"},{"issue":"18","key":"10.1016\/j.bbrc.2022.04.104_bib30","doi-asserted-by":"crossref","first-page":"3338","DOI":"10.1111\/febs.13813","article-title":"The cytosolic cochaperone Sti1 is relevant for mitochondrial biogenesis and morphology","volume":"283","author":"Hoseini","year":"2016","journal-title":"FEBS J."},{"issue":"9","key":"10.1016\/j.bbrc.2022.04.104_bib31","doi-asserted-by":"crossref","first-page":"3091","DOI":"10.1083\/jcb.201712029","article-title":"Cytosolic Hsp70 and Hsp40 chaperones enable the biogenesis of mitochondrial beta-barrel proteins","volume":"217","author":"Jores","year":"2018","journal-title":"J.\u00a0Cell Biol."},{"issue":"20","key":"10.1016\/j.bbrc.2022.04.104_bib32","doi-asserted-by":"crossref","first-page":"6750","DOI":"10.1093\/nar\/gkm777","article-title":"Discovery of a mRNA mitochondrial localization element in Saccharomyces cerevisiae by nonhomologous random recombination and in\u00a0vivo selection","volume":"35","author":"Liu","year":"2007","journal-title":"Nucleic Acids Res."},{"issue":"1","key":"10.1016\/j.bbrc.2022.04.104_bib33","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1093\/jb\/mvj136","article-title":"Mutational and functional analysis of the cryptic N-terminal targeting signal for both mitochondria and peroxisomes in yeast peroxisomal citrate synthase Cit2p","volume":"140","author":"Lee","year":"2006","journal-title":"J.\u00a0Biochem."},{"issue":"24","key":"10.1016\/j.bbrc.2022.04.104_bib34","doi-asserted-by":"crossref","first-page":"6893","DOI":"10.1093\/emboj\/cdf690","article-title":"In Saccharomyces cerevisiae, ATP2 mRNA sorting to the vicinity of mitochondria is essential for respiratory function","volume":"21","author":"Margeot","year":"2002","journal-title":"EMBO J."},{"issue":"5","key":"10.1016\/j.bbrc.2022.04.104_bib35","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1002\/jcb.22200","article-title":"Identification and characterization of the mitochondrial targeting sequence and mechanism in human citrate synthase","volume":"107","author":"Cheng","year":"2009","journal-title":"J.\u00a0Cell. Biochem."},{"issue":"13","key":"10.1016\/j.bbrc.2022.04.104_bib36","doi-asserted-by":"crossref","first-page":"4680","DOI":"10.1021\/bi00413a015","article-title":"Isolation, nucleotide sequence, and expression of a cDNA encoding pig citrate synthase","volume":"27","author":"Evans","year":"1988","journal-title":"Biochemistry"}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X22006465?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X22006465?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T12:18:29Z","timestamp":1757765909000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X22006465"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7]]},"references-count":36,"alternative-id":["S0006291X22006465"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2022.04.104","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2022,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"H55\u00a0N variation in citrate synthase leads to decrement in the enzyme activity and transport rate to mitochondria in HEI-OC1 cells","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2022.04.104","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}