{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T23:22:52Z","timestamp":1782256972343,"version":"3.54.5"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T00:00:00Z","timestamp":1667952000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP19fk0108035"],"award-info":[{"award-number":["JP19fk0108035"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2022,12]]},"DOI":"10.1016\/j.bbrc.2022.11.013","type":"journal-article","created":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T11:09:02Z","timestamp":1667992142000},"page":"181-188","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Identification of the corticotropin-releasing factor receptor 1 antagonists as inhibitors of Chikungunya virus replication using a Gaussia luciferase\u2013expressing subgenomic replicon"],"prefix":"10.1016","volume":"637","author":[{"given":"Yasuo","family":"Watanabe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3554-0755","authenticated-orcid":false,"given":"Youichi","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akino","family":"Emi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5905-532X","authenticated-orcid":false,"given":"Takeshi","family":"Murakawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Hishiki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumihiro","family":"Kato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3650-1221","authenticated-orcid":false,"given":"Shoichi","family":"Sakaguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takato","family":"Yano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5823-8300","authenticated-orcid":false,"given":"Chang-Kweng","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohiko","family":"Takasaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5928-6723","authenticated-orcid":false,"given":"Takashi","family":"Nakano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2022.11.013_bib1","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.antiviral.2015.07.002","article-title":"Chikungunya virus pathogenesis: from bedside to bench","volume":"121","author":"Couderc","year":"2015","journal-title":"Antivir. Res."},{"key":"10.1016\/j.bbrc.2022.11.013_bib2","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.virol.2009.07.024","article-title":"Replication cycle of chikungunya: a re-emerging arbovirus","volume":"393","author":"Solignat","year":"2009","journal-title":"Virology"},{"key":"10.1016\/j.bbrc.2022.11.013_bib3","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/bs.aivir.2021.07.003","article-title":"Alphavirus RNA replication in vertebrate cells","volume":"111","author":"Ahola","year":"2021","journal-title":"Adv. Virus Res."},{"key":"10.1016\/j.bbrc.2022.11.013_bib4","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.coviro.2017.03.016","article-title":"Chikungunya virus infections: time to act, time to treat","volume":"24","author":"Abdelnabi","year":"2017","journal-title":"Curr. Opin. Virol."},{"key":"10.1016\/j.bbrc.2022.11.013_bib5","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.antiviral.2015.04.016","article-title":"Chikungunya: evolutionary history and recent epidemic spread","volume":"120","author":"Weaver","year":"2015","journal-title":"Antivir. Res."},{"key":"10.1016\/j.bbrc.2022.11.013_bib6","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.coviro.2016.09.014","article-title":"Chikungunya: an unexpected emergence in Europe","volume":"21","author":"Amraoui","year":"2016","journal-title":"Curr. Opin. Virol."},{"key":"10.1016\/j.bbrc.2022.11.013_bib7","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1097\/QCO.0b013e32833c1d16","article-title":"Dengue and chikungunya infections in travelers","volume":"23","author":"Chen","year":"2010","journal-title":"Curr. Opin. Infect. Dis."},{"key":"10.1016\/j.bbrc.2022.11.013_bib8","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.antiviral.2015.06.017","article-title":"Towards antivirals against chikungunya virus","volume":"121","author":"Abdelnabi","year":"2015","journal-title":"Antivir. Res."},{"key":"10.1016\/j.bbrc.2022.11.013_bib9","doi-asserted-by":"crossref","first-page":"209","DOI":"10.7883\/yoken.67.209","article-title":"Development of a novel Dengue-1 virus replicon system expressing secretory Gaussia luciferase for analysis of viral replication and discovery of antiviral drugs","volume":"67","author":"Kato","year":"2014","journal-title":"Jpn. J. Infect. Dis."},{"key":"10.1016\/j.bbrc.2022.11.013_bib10","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.virol.2020.09.006","article-title":"Construction and characterization of an infectious clone generated from Chikungunya virus SL11131 strain","volume":"552","author":"Suzuki","year":"2021","journal-title":"Virology"},{"key":"10.1016\/j.bbrc.2022.11.013_bib11","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0151616","article-title":"Versatile trans-replication systems for Chikungunya virus allow functional analysis and tagging of every replicase protein","volume":"11","author":"Utt","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.bbrc.2022.11.013_bib12","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1016\/j.bbrc.2008.09.040","article-title":"RNA interference mediated inhibition of Chikungunya virus replication in mammalian cells","volume":"376","author":"Dash","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2022.11.013_bib13","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1005357","article-title":"Characterization of RyDEN (C19orf66) as an interferon-stimulated cellular inhibitor against dengue virus replication","volume":"12","author":"Suzuki","year":"2016","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.bbrc.2022.11.013_bib14","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.jviromet.2014.01.008","article-title":"Development and characterization of monoclonal antibody against non-structural protein-2 of Chikungunya virus and its application","volume":"199","author":"Chattopadhyay","year":"2014","journal-title":"J.\u00a0Virol. Methods"},{"key":"10.1016\/j.bbrc.2022.11.013_bib15","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.virol.2008.09.008","article-title":"A\u00a0cell-based luciferase assay amenable to high-throughput screening of inhibitors of arenavirus budding","volume":"382","author":"Capul","year":"2008","journal-title":"Virology"},{"key":"10.1016\/j.bbrc.2022.11.013_bib16","doi-asserted-by":"crossref","DOI":"10.3791\/58407","article-title":"Measuring dengue virus RNA in the culture supernatant of infected cells by real-time quantitative polymerase chain reaction","author":"Suzuki","year":"2018","journal-title":"JoVE"},{"key":"10.1016\/j.bbrc.2022.11.013_bib17","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0028923","article-title":"Inhibitors of alphavirus entry and replication identified with a stable Chikungunya replicon cell line and virus-based assays","volume":"6","author":"Pohjala","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.bbrc.2022.11.013_bib18","doi-asserted-by":"crossref","first-page":"3145","DOI":"10.1128\/JVI.03213-14","article-title":"Mutations conferring a noncytotoxic phenotype on chikungunya virus replicons compromise enzymatic properties of nonstructural protein 2","volume":"89","author":"Utt","year":"2015","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.bbrc.2022.11.013_bib19","doi-asserted-by":"crossref","first-page":"1943","DOI":"10.1128\/JVI.79.3.1943-1947.2005","article-title":"The predominant mechanism by which ribavirin exerts its antiviral activity in\u00a0vitro against flaviviruses and paramyxoviruses is mediated by inhibition of IMP dehydrogenase","volume":"79","author":"Leyssen","year":"2005","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.bbrc.2022.11.013_bib20","doi-asserted-by":"crossref","first-page":"3145","DOI":"10.1038\/s41598-017-03316-5","article-title":"Imipramine inhibits Chikungunya virus replication in human skin fibroblasts through interference with intracellular cholesterol trafficking","volume":"7","author":"Wichit","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bbrc.2022.11.013_bib21","doi-asserted-by":"crossref","first-page":"10477","DOI":"10.1073\/pnas.93.19.10477","article-title":"CP-154,526: a potent and selective nonpeptide antagonist of corticotropin releasing factor receptors","volume":"93","author":"Schulz","year":"1996","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.bbrc.2022.11.013_bib22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1159\/000076041","article-title":"Hypothalamic-pituitary-adrenocortical system and mood disorders: highlights from mutant mice","volume":"79","author":"Muller","year":"2004","journal-title":"Neuroendocrinology"},{"key":"10.1016\/j.bbrc.2022.11.013_bib23","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1007\/s00213-017-4556-2","article-title":"Don't stress about CRF: assessing the translational failures of CRF1antagonists","volume":"234","author":"Spierling","year":"2017","journal-title":"Psychopharmacology (Berl)"},{"key":"10.1016\/j.bbrc.2022.11.013_bib24","doi-asserted-by":"crossref","first-page":"4787","DOI":"10.1021\/jm040058e","article-title":"Design of 2,5-dimethyl-3-(6-dimethyl-4-methylpyridin-3-yl)-7-dipropylaminopyrazolo[1,5-a]pyrimidine (NBI 30775\/R121919) and structure--activity relationships of a series of potent and orally active corticotropin-releasing factor receptor antagonists","volume":"47","author":"Chen","year":"2004","journal-title":"J.\u00a0Med. Chem."},{"key":"10.1016\/j.bbrc.2022.11.013_bib25","doi-asserted-by":"crossref","first-page":"4187","DOI":"10.1021\/jm200365y","article-title":"Discovery of N-(1-ethylpropyl)-[3-methoxy-5-(2-methoxy-4-trifluoromethoxyphenyl)-6-methyl-pyra zin-2-yl]amine 59 (NGD 98-2): an orally active corticotropin releasing factor-1 (CRF-1) receptor antagonist","volume":"54","author":"Hodgetts","year":"2011","journal-title":"J.\u00a0Med. Chem."},{"key":"10.1016\/j.bbrc.2022.11.013_bib26","doi-asserted-by":"crossref","first-page":"598","DOI":"10.3390\/v12060598","article-title":"Reporter replicons for antiviral drug discovery against positive single-stranded RNA viruses","volume":"12","author":"Fernandes","year":"2020","journal-title":"Viruses"},{"key":"10.1016\/j.bbrc.2022.11.013_bib27","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.antiviral.2015.12.012","article-title":"Discovery of berberine, abamectin and ivermectin as antivirals against chikungunya and other alphaviruses","volume":"126","author":"Varghese","year":"2016","journal-title":"Antivir. Res."},{"key":"10.1016\/j.bbrc.2022.11.013_bib28","doi-asserted-by":"crossref","first-page":"11421","DOI":"10.1038\/srep11421","article-title":"Antiviral activity of silymarin against chikungunya virus","volume":"5","author":"Lani","year":"2015","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bbrc.2022.11.013_bib29","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.antiviral.2015.06.013","article-title":"Suramin inhibits chikungunya virus replication through multiple mechanisms","volume":"121","author":"Albulescu","year":"2015","journal-title":"Antivir. Res."},{"key":"10.1016\/j.bbrc.2022.11.013_bib30","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.antiviral.2016.08.014","article-title":"High-throughput screening for the identification of small-molecule inhibitors of the flaviviral protease","volume":"134","author":"Balasubramanian","year":"2016","journal-title":"Antivir. Res."},{"key":"10.1016\/j.bbrc.2022.11.013_bib31","doi-asserted-by":"crossref","first-page":"504","DOI":"10.3389\/fmicb.2020.00504","article-title":"Tolcapone potently inhibits seminal amyloid fibrils formation and blocks entry of ebola pseudoviruses","volume":"11","author":"Qiu","year":"2020","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.bbrc.2022.11.013_bib32","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/0042-6822(60)90011-8","article-title":"Suppressive effects of pyrilamine maleate and d-lysergic acid diethylamide (LSD-25) on early corneal lesions produced in\u00a0vitro by Newcastle disease virus (NDV) and compound 48\/80","volume":"10","author":"Oh","year":"1960","journal-title":"Virology"},{"key":"10.1016\/j.bbrc.2022.11.013_bib33","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1159\/000328226","article-title":"The corticotropin-releasing hormone network and the hypothalamic-pituitary-adrenal axis: molecular and cellular mechanisms involved","volume":"94","author":"Bonfiglio","year":"2011","journal-title":"Neuroendocrinology"}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X22015479?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X22015479?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T14:46:02Z","timestamp":1762094762000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X22015479"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12]]},"references-count":33,"alternative-id":["S0006291X22015479"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2022.11.013","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2022,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identification of the corticotropin-releasing factor receptor 1 antagonists as inhibitors of Chikungunya virus replication using a Gaussia luciferase\u2013expressing subgenomic replicon","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2022.11.013","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}