{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:44:18Z","timestamp":1790775858486,"version":"4.1.0"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003621","name":"Ministry of Science, ICT and Future Planning","doi-asserted-by":"publisher","award":["- 2017M3A9G6068246"],"award-info":[{"award-number":["- 2017M3A9G6068246"]}],"id":[{"id":"10.13039\/501100003621","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003621","name":"Ministry of Science, ICT and Future Planning","doi-asserted-by":"publisher","award":["2017R1D1A1B03033204"],"award-info":[{"award-number":["2017R1D1A1B03033204"]}],"id":[{"id":"10.13039\/501100003621","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003621","name":"Ministry of Science, ICT and Future Planning","doi-asserted-by":"publisher","award":["2020R1A2C1101104"],"award-info":[{"award-number":["2020R1A2C1101104"]}],"id":[{"id":"10.13039\/501100003621","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2022,12]]},"DOI":"10.1016\/j.bbrc.2022.11.044","type":"journal-article","created":{"date-parts":[[2022,11,17]],"date-time":"2022-11-17T03:21:28Z","timestamp":1668655288000},"page":"308-313","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["Discovery of novel Leishmania major trypanothione synthetase inhibitors by high-throughput screening"],"prefix":"10.1016","volume":"637","author":[{"given":"Trong-Nhat","family":"Phan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9493-9577","authenticated-orcid":false,"given":"Kyuho Paul","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4639-8539","authenticated-orcid":false,"given":"Diego","family":"Ben\u00edtez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5000-1333","authenticated-orcid":false,"given":"Marcelo A.","family":"Comini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Shum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joo Hwan","family":"No","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.bbrc.2022.11.044_bib1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0035671","article-title":"Leishmaniasis worldwide and global estimates of its incidence","volume":"7","author":"Alvar","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.bbrc.2022.11.044_bib2","volume":"375","year":"2017","journal-title":"Leishmaniasis. Fact sheet"},{"key":"10.1016\/j.bbrc.2022.11.044_bib3","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.02851","article-title":"Global distribution maps of the leishmaniases","volume":"3","author":"Pigott","year":"2014","journal-title":"Elife"},{"key":"10.1016\/j.bbrc.2022.11.044_bib4","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.actatropica.2015.12.016","article-title":"Visceral leishmaniasis: revisiting current treatments and approaches for future discoveries","volume":"155","author":"No","year":"2016","journal-title":"Acta Trop."},{"issue":"8","key":"10.1016\/j.bbrc.2022.11.044_bib6","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.pt.2008.04.006","article-title":"Ever-increasing complexities of diamidine and arsenical cross resistance in African trypanosomes","volume":"24","author":"de Koning","year":"2008","journal-title":"Trends Parasitol."},{"issue":"13","key":"10.1016\/j.bbrc.2022.11.044_bib7","doi-asserted-by":"crossref","first-page":"5022","DOI":"10.1073\/pnas.0711014105","article-title":"A\u00a0mechanism for cross-resistance to nifurtimox and benznidazole in trypanosomes","volume":"105","author":"Wilkinson","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"9","key":"10.1016\/j.bbrc.2022.11.044_bib8","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1007\/s40265-013-0069-4","article-title":"Amphotericin B formulations: a comparative review of efficacy and toxicity","volume":"73","author":"Hamill","year":"2013","journal-title":"Drugs"},{"issue":"12","key":"10.1016\/j.bbrc.2022.11.044_bib9","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0006052","article-title":"Drug resistance and treatment failure in leishmaniasis: a 21st century challenge","volume":"11","author":"Ponte-Sucre","year":"2017","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.bbrc.2022.11.044_bib10","series-title":"Trypanosomatid Diseases: Molecular Routes to Drug Discovery","author":"Jager","year":"2013"},{"issue":"4693","key":"10.1016\/j.bbrc.2022.11.044_bib11","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1126\/science.3883489","article-title":"Trypanothione: a novel bis(glutathionyl)spermidine cofactor for glutathione reductase in trypanosomatids","volume":"227","author":"Fairlamb","year":"1985","journal-title":"Science"},{"issue":"25","key":"10.1016\/j.bbrc.2022.11.044_bib12","doi-asserted-by":"crossref","first-page":"17672","DOI":"10.1074\/jbc.M801850200","article-title":"Leishmania trypanothione synthetase-amidase structure reveals a basis for regulation of conflicting synthetic and hydrolytic activities","volume":"283","author":"Fyfe","year":"2008","journal-title":"J.\u00a0Biol. Chem."},{"issue":"18","key":"10.1016\/j.bbrc.2022.11.044_bib13","doi-asserted-by":"crossref","first-page":"11908","DOI":"10.1074\/jbc.272.18.11908","article-title":"Convenient isolation and kinetic mechanism of glutathionylspermidine synthetase from Crithidia fasciculata","volume":"272","author":"Koenig","year":"1997","journal-title":"J.\u00a0Biol. Chem."},{"issue":"Pt 3","key":"10.1016\/j.bbrc.2022.11.044_bib14","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1042\/bj20011370","article-title":"Characterization of recombinant glutathionylspermidine synthetase\/amidase from Crithidia fasciculata","volume":"364","author":"Oza","year":"2002","journal-title":"Biochem. J."},{"key":"10.1016\/j.bbrc.2022.11.044_bib15","series-title":"A\u00a0Revision on the Field: Biosynthesis of Polyamine\u2013Glutathione Derivatives in Enterobacteria and Kinetoplastida. Book: Glutathione","first-page":"285","author":"Comini","year":"2018"},{"issue":"10","key":"10.1016\/j.bbrc.2022.11.044_bib16","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1016\/j.freeradbiomed.2004.02.008","article-title":"Validation of Trypanosoma brucei trypanothione synthetase as drug target","volume":"36","author":"Comini","year":"2004","journal-title":"Free Radic. Biol. Med."},{"issue":"Pt 2","key":"10.1016\/j.bbrc.2022.11.044_bib17","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1042\/BJ20050911","article-title":"Phenotypic analysis of trypanothione synthetase knockdown in the African trypanosome","volume":"391","author":"Ariyanayagam","year":"2005","journal-title":"Biochem. J."},{"issue":"3","key":"10.1016\/j.bbrc.2022.11.044_bib18","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1111\/j.1365-2958.2009.06761.x","article-title":"Dissecting the essentiality of the bifunctional trypanothione synthetase-amidase in Trypanosoma brucei using chemical and genetic methods","volume":"74","author":"Wyllie","year":"2009","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.bbrc.2022.11.044_bib19","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.freeradbiomed.2014.05.007","article-title":"Genetic and chemical analyses reveal that trypanothione synthetase but not glutathionylspermidine synthetase is essential for Leishmania infantum","volume":"73","author":"Sousa","year":"2014","journal-title":"Free Radic. Biol. Med."},{"issue":"1","key":"10.1016\/j.bbrc.2022.11.044_bib20","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1021\/jm050922i","article-title":"Design, synthesis, and biological evaluation of phosphinopeptides against Trypanosoma cruzi targeting trypanothione biosynthesis","volume":"49","author":"Ravaschino","year":"2006","journal-title":"J.\u00a0Med. Chem."},{"issue":"52","key":"10.1016\/j.bbrc.2022.11.044_bib21","doi-asserted-by":"crossref","first-page":"36137","DOI":"10.1074\/jbc.M109.045336","article-title":"Chemical validation of trypanothione synthetase: a potential drug target for human trypanosomiasis","volume":"284","author":"Torrie","year":"2009","journal-title":"J.\u00a0Biol. Chem."},{"issue":"21","key":"10.1016\/j.bbrc.2022.11.044_bib22","doi-asserted-by":"crossref","first-page":"5408","DOI":"10.1111\/j.1742-4658.2008.06670.x","article-title":"ATP-dependent ligases in trypanothione biosynthesis--kinetics of catalysis and inhibition by phosphinic acid pseudopeptides","volume":"275","author":"Oza","year":"2008","journal-title":"FEBS J."},{"issue":"1","key":"10.1016\/j.bbrc.2022.11.044_bib23","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1002\/cmdc.201100420","article-title":"Design, synthesis and biological evaluation of Trypanosoma brucei trypanothione synthetase inhibitors","volume":"7","author":"Spinks","year":"2012","journal-title":"ChemMedChem"},{"issue":"4","key":"10.1016\/j.bbrc.2022.11.044_bib24","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004617","article-title":"Identification of novel chemical scaffolds inhibiting trypanothione synthetase from pathogenic trypanosomatids","volume":"10","author":"Benitez","year":"2016","journal-title":"PLoS Neglected Trop. Dis."},{"issue":"1","key":"10.1016\/j.bbrc.2022.11.044_bib25","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1080\/14756366.2020.1780227","article-title":"Mechanistic and biological characterisation of novel N(5)-substituted paullones targeting the biosynthesis of trypanothione in Leishmania","volume":"35","author":"Medeiros","year":"2020","journal-title":"J.\u00a0Enzym. Inhib. Med. Chem."},{"issue":"1","key":"10.1016\/j.bbrc.2022.11.044_bib26","doi-asserted-by":"crossref","first-page":"912","DOI":"10.1080\/14756366.2022.2045590","article-title":"Drug-like molecules with anti-trypanothione synthetase activity identified by high throughput screening","volume":"37","author":"Ben\u00edtez","year":"2022","journal-title":"J.\u00a0Enzym. Inhib. Med. Chem."},{"issue":"8","key":"10.1016\/j.bbrc.2022.11.044_bib27","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.3390\/molecules25081980","article-title":"In\u00a0vitro and in\u00a0vivo activity of mTOR kinase and PI3K inhibitors against Leishmania donovani and Trypanosoma brucei","volume":"25","author":"Phan","year":"2020","journal-title":"Molecules"},{"issue":"24","key":"10.1016\/j.bbrc.2022.11.044_bib28","doi-asserted-by":"crossref","first-page":"5970","DOI":"10.1038\/sj.emboj.7601440","article-title":"Dual binding sites for translocation catalysis by Escherichia coli glutathionylspermidine synthetase","volume":"25","author":"Pai","year":"2006","journal-title":"EMBO J."},{"issue":"3","key":"10.1016\/j.bbrc.2022.11.044_bib29","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.annder.2019.02.002","article-title":"Cutaneous leishmaniasis","volume":"146","author":"Mokni","year":"2019","journal-title":"Ann. Dermatol. Venereol."},{"issue":"24","key":"10.1016\/j.bbrc.2022.11.044_bib30","doi-asserted-by":"crossref","first-page":"2420","DOI":"10.1002\/cmdc.202000325","article-title":"Recent advancement in the search of innovative antiprotozoal agents targeting trypanothione metabolism","volume":"15","author":"Saccoliti","year":"2020","journal-title":"ChemMedChem"},{"issue":"2","key":"10.1016\/j.bbrc.2022.11.044_bib31","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0056788","article-title":"Molecular dynamics reveal binding mode of glutathionylspermidine by trypanothione synthetase","volume":"8","author":"Koch","year":"2013","journal-title":"PLoS One"},{"issue":"23","key":"10.1016\/j.bbrc.2022.11.044_bib32","doi-asserted-by":"crossref","first-page":"3881","DOI":"10.1002\/1873-3468.12904","article-title":"Buthionine sulfoximine is a multitarget inhibitor of trypanothione synthesis in Trypanosoma cruzi","volume":"591","author":"V\u00e1zquez","year":"2017","journal-title":"FEBS Lett."}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X22015935?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X22015935?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T14:45:48Z","timestamp":1762094748000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X22015935"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12]]},"references-count":31,"alternative-id":["S0006291X22015935"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2022.11.044","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2022,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Discovery of novel Leishmania major trypanothione synthetase inhibitors by high-throughput screening","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2022.11.044","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}