{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T14:50:25Z","timestamp":1762095025205,"version":"build-2065373602"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81661168016","81770786","81870537","82070816"],"award-info":[{"award-number":["81661168016","81770786","81870537","82070816"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018625","name":"Science and Technology Innovation Plan Of Shanghai Science and Technology Commission","doi-asserted-by":"publisher","award":["19140904200"],"award-info":[{"award-number":["19140904200"]}],"id":[{"id":"10.13039\/501100018625","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2023,1]]},"DOI":"10.1016\/j.bbrc.2022.12.033","type":"journal-article","created":{"date-parts":[[2022,12,12]],"date-time":"2022-12-12T10:57:41Z","timestamp":1670842661000},"page":"21-26","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Large-scale forward genetic screening of zebrafish affecting thyroid development"],"prefix":"10.1016","volume":"642","author":[{"given":"Jia-Ping","family":"Wan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cao-Xu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya","family":"Fang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen-Yan","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng-Yao","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang-Xia","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9609-5803","authenticated-orcid":false,"given":"Huai-Dong","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1927-0791","authenticated-orcid":false,"given":"Mei","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"10","key":"10.1016\/j.bbrc.2022.12.033_bib1","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1001\/jamapediatrics.2016.0486","article-title":"Thyroid disorders in children and adolesces A review [J]","volume":"170","author":"Hanley","year":"2016","journal-title":"JAMA Pediatr."},{"issue":"17","key":"10.1016\/j.bbrc.2022.12.033_bib2","article-title":"Congenital hypothyroidism [J]","volume":"5","author":"Rastogi","year":"2010","journal-title":"Orphanet J. Rare Dis."},{"key":"10.1016\/j.bbrc.2022.12.033_bib3","first-page":"79","article-title":"Newborn screening for inborn errors of metabolism in mainland China: 30 years of experience [J]","volume":"6","author":"Shi","year":"2012","journal-title":"JIMD Rep."},{"issue":"12 Suppl","key":"10.1016\/j.bbrc.2022.12.033_bib4","article-title":"Newborn screening in China: phenylketonuria, congenital hypothyroidism and expanded screening [J]","volume":"37","author":"Gu","year":"2008","journal-title":"Ann. Acad. Med. Singapore"},{"issue":"12","key":"10.1016\/j.bbrc.2022.12.033_bib5","doi-asserted-by":"crossref","first-page":"2123","DOI":"10.1242\/dev.145615","article-title":"Development of the thyroid gland [J]","volume":"144","author":"Nilsson","year":"2017","journal-title":"Development"},{"issue":"2","key":"10.1016\/j.bbrc.2022.12.033_bib6","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.ecl.2016.01.007","article-title":"Update of thyroid developmental genes [J]","volume":"45","author":"Stoupa","year":"2016","journal-title":"Endocrinol Metab. Clin. N. Am."},{"issue":"5","key":"10.1016\/j.bbrc.2022.12.033_bib7","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1210\/er.2003-0028","article-title":"Thyroid development and its disorders: genetics and molecular mechanisms [J]","volume":"25","author":"De Felice","year":"2004","journal-title":"Endocr. Rev."},{"issue":"2","key":"10.1016\/j.bbrc.2022.12.033_bib8","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.ecl.2017.01.005","article-title":"Defects of thyroid hormone synthesis and action [J]","volume":"46","author":"Hannoush","year":"2017","journal-title":"Endocrinol Metab. Clin. N. Am."},{"issue":"1","key":"10.1016\/j.bbrc.2022.12.033_bib9","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1111\/cge.13627","article-title":"Molecular defects in thyroid dysgenesis [J]","volume":"97","author":"Mio","year":"2020","journal-title":"Clin. Genet."},{"issue":"3","key":"10.1016\/j.bbrc.2022.12.033_bib10","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1089\/thy.1997.7.377","article-title":"Absence of mutations in the gene encoding thyroid transcription factor-1 (TTF-1) in patients with thyroid dysgenesis [J]","volume":"7","author":"Perna","year":"1997","journal-title":"Thyroid"},{"issue":"2","key":"10.1016\/j.bbrc.2022.12.033_bib11","doi-asserted-by":"crossref","first-page":"172","DOI":"10.2478\/folmed-2018-0078","article-title":"Genetics of thyroid disorders [J]","volume":"61","author":"Cortes","year":"2019","journal-title":"Folia Med."},{"issue":"6","key":"10.1016\/j.bbrc.2022.12.033_bib12","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1159\/000281438","article-title":"Spectrum of human foxe1\/TTF2 mutations [J]","volume":"73","author":"Castanet","year":"2010","journal-title":"Horm. Res. Paediatr."},{"issue":"2","key":"10.1016\/j.bbrc.2022.12.033_bib13","first-page":"225","article-title":"Genetics of primary congenital hypothyroidism-a review [J]","volume":"20","author":"Kostopoulou","year":"2021","journal-title":"Horm. Int. J. Endocrinol. Metabol."},{"issue":"Suppl 2","key":"10.1016\/j.bbrc.2022.12.033_bib14","first-page":"185","article-title":"Congenital hypothyroidism clinical aspects and late consequences [J]","volume":"1","author":"Buyukgebiz","year":"2003","journal-title":"Pediatr. Endocrinol. Rev."},{"issue":"5","key":"10.1016\/j.bbrc.2022.12.033_bib15","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1038\/nrg2091","article-title":"Animal models of human disease: zebrafish swim into view [J]","volume":"8","author":"Lieschke","year":"2007","journal-title":"Nat. Rev. Genet."},{"issue":"1","key":"10.1016\/j.bbrc.2022.12.033_bib16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1242\/dev.123.1.1","article-title":"The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio [J]","volume":"123","author":"Haffter","year":"1996","journal-title":"Development (Camb.)"},{"issue":"1","key":"10.1016\/j.bbrc.2022.12.033_bib17","article-title":"Hematopoietic mutations in the zebrafish [J]","volume":"123","author":"Weinstein","year":"1996","journal-title":"Development (Camb.)"},{"issue":"3","key":"10.1016\/j.bbrc.2022.12.033_bib18","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1016\/j.bbrc.2015.03.119","article-title":"Analysis of mutants from a genetic screening reveals the control of intestine and liver development by many common genes in zebrafish [J]","volume":"460","author":"Jiang","year":"2015","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2022.12.033_bib19","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.ydbio.2020.11.009","article-title":"Postembryonic screen for mutations affecting spine development in zebra fish [J]","volume":"471","author":"Gray","year":"2021","journal-title":"Dev. Biol."},{"issue":"3","key":"10.1016\/j.bbrc.2022.12.033_bib20","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1038\/nbt.2501","article-title":"Efficient genome editing in zebrafish using a CRISPR-Cas system [J]","volume":"31","author":"Hwang","year":"2013","journal-title":"Nat. Biotechnol."},{"issue":"1","key":"10.1016\/j.bbrc.2022.12.033_bib21","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.devcel.2011.06.007","article-title":"Forward and reverse genetic approaches for the analysis of vertebrate development in the zebrafish [J]","volume":"21","author":"Lawson","year":"2011","journal-title":"Dev. Cell"},{"issue":"1","key":"10.1016\/j.bbrc.2022.12.033_bib22","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.ydbio.2005.07.013","article-title":"Formation of the digestive system in zebrafish: III. Intestinal epithelium morphogenesis [J]","volume":"286","author":"Ng","year":"2005","journal-title":"Dev. Biol."},{"issue":"6","key":"10.1016\/j.bbrc.2022.12.033_bib23","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/S1673-8527(08)60121-6","article-title":"Liver development in zebrafish (Danio rerio) [J]","volume":"36","author":"Tao","year":"2009","journal-title":"J.\u00a0Gen. Genom."},{"issue":"1","key":"10.1016\/j.bbrc.2022.12.033_bib24","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/S0012-1606(03)00436-6","article-title":"Zebrafish hhex, nk2.1a, and pax2.1 regulate thyroid growth and differentiation downstream of Nodal-dependent transcription factors [J]","volume":"263","author":"Elsalini","year":"2003","journal-title":"Dev. Biol."},{"issue":"1\u20132","key":"10.1016\/j.bbrc.2022.12.033_bib25","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/S0925-4773(00)00345-2","article-title":"Expression of nk2.1a during early development of the thyroid gland in zebrafish [J]","volume":"95","author":"Rohr","year":"2000","journal-title":"Mech. Dev."},{"issue":"9","key":"10.1016\/j.bbrc.2022.12.033_bib26","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.1210\/me.2011-0046","article-title":"TSH receptor function is required for normal thyroid differentiation in zebrafish [J]","volume":"25","author":"Opitz","year":"2011","journal-title":"Mol. Endocrinol."},{"issue":"16","key":"10.1016\/j.bbrc.2022.12.033_bib27","doi-asserted-by":"crossref","DOI":"10.1242\/dev.125.16.3063","article-title":"Characterization of three novel members of the zebrafish Pax2\/5\/8 family: dependency of Pax5 and Pax8 expression on the Pax2.1 (noi) function [J]","volume":"125","author":"Pfeffer","year":"1998","journal-title":"Development"},{"key":"10.1016\/j.bbrc.2022.12.033_bib28","article-title":"Tpo knockout in zebrafish partially recapitulates clinical manifestations of congenital hypothyroidism and reveals the involvement of TH in proper development of glucose homeostasis [J]","volume":"323","author":"Fang","year":"2022","journal-title":"Gen. Comp. Endocrinol."},{"issue":"8","key":"10.1016\/j.bbrc.2022.12.033_bib29","doi-asserted-by":"crossref","DOI":"10.1210\/endocr\/bqab101","article-title":"Genetic manipulation on zebrafish duox recapitulate the clinical manifestations of congenital hypothyroidism [J]","volume":"162","author":"Sun","year":"2021","journal-title":"Endocrinology"},{"issue":"1\u20132","key":"10.1016\/j.bbrc.2022.12.033_bib30","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.mce.2009.05.011","article-title":"Thyroid gland development and function in the zebrafish model [J]","volume":"312","author":"Porazzi","year":"2009","journal-title":"Mol. Cell. Endocrinol."},{"issue":"1","key":"10.1016\/j.bbrc.2022.12.033_bib31","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1172\/JCI70038","article-title":"Embryonic exposure to excess thyroid hormone causes thyrotrope cell death [J]","volume":"124","author":"Tonyushkina","year":"2014","journal-title":"J.\u00a0Clin. Invest."},{"key":"10.1016\/j.bbrc.2022.12.033_bib32","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-1-60327-977-2_1","article-title":"Highly efficient ENU mutagenesis in zebrafish [J]","volume":"546","author":"De Bruijn","year":"2009","journal-title":"Zebrafish: Method. Protocols"},{"issue":"3","key":"10.1016\/j.bbrc.2022.12.033_bib33","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1002\/aja.1002030302","article-title":"Stages of embryonic development of the Zebrafish [J]","volume":"203","author":"Kimmel","year":"1995","journal-title":"Dev. Dynam."},{"issue":"1","key":"10.1016\/j.bbrc.2022.12.033_bib34","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1242\/dev.123.1.37","article-title":"A\u00a0genetic screen for mutations affecting embryogenesis","volume":"123","author":"Driever","year":"1996","journal-title":"Development (Camb.)"},{"issue":"4","key":"10.1016\/j.bbrc.2022.12.033_bib35","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.bcmd.2013.07.009","article-title":"Assaying hematopoiesis using zebrafish [J]","volume":"51","author":"Boatman","year":"2013","journal-title":"Blood Cells Mol. Dis."},{"issue":"4","key":"10.1016\/j.bbrc.2022.12.033_bib36","first-page":"493","article-title":"Pathways in blood and vessel development revealed through zebrafish genetics [J]","volume":"46","author":"Crosier","year":"2002","journal-title":"Int. J. Dev. Biol."},{"volume":"vol. 40","year":"2010","author":"James","key":"10.1016\/j.bbrc.2022.12.033_bib37"},{"issue":"5","key":"10.1016\/j.bbrc.2022.12.033_bib38","first-page":"322","article-title":"The clinical approach to diagnosis and treatment of hypothyroidism [J]","volume":"156","author":"Bashkin","year":"2017","journal-title":"Harefuah"},{"issue":"6","key":"10.1016\/j.bbrc.2022.12.033_bib39","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1007\/s1012601-0053-4","article-title":"Generating transparent zebrafish: a refined method to improve detection of gene expression during embryonic development [J]","volume":"3","author":"Karlsson","year":"2001","journal-title":"Mar. Biotechnol."},{"issue":"3","key":"10.1016\/j.bbrc.2022.12.033_bib40","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1007\/s10695-018-0488-y","article-title":"Intrafollicular thyroid hormone staining in whole-mount zebrafish (Danio rerio) embryos for the detection of thyroid hormone synthesis disruption [J]","volume":"44","author":"Rehberger","year":"2018","journal-title":"Fish Physiol. Biochem."},{"issue":"35","key":"10.1016\/j.bbrc.2022.12.033_bib41","doi-asserted-by":"crossref","first-page":"12792","DOI":"10.1073\/pnas.0403929101","article-title":"Identification of 315 genes essential for early zebrafish development [J]","volume":"101","author":"Amsterdam","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U. S. A"}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X22016989?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X22016989?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T14:45:31Z","timestamp":1762094731000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X22016989"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1]]},"references-count":41,"alternative-id":["S0006291X22016989"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2022.12.033","relation":{},"ISSN":["0006-291X"],"issn-type":[{"type":"print","value":"0006-291X"}],"subject":[],"published":{"date-parts":[[2023,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Large-scale forward genetic screening of zebrafish affecting thyroid development","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2022.12.033","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}