{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:59:35Z","timestamp":1787241575908,"version":"build-2736575974"},"reference-count":22,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T00:00:00Z","timestamp":1685491200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014974","name":"Institute of Biophysics Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["2019YFA0110400"],"award-info":[{"award-number":["2019YFA0110400"]}],"id":[{"id":"10.13039\/501100014974","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2023,8]]},"DOI":"10.1016\/j.bbrc.2023.05.104","type":"journal-article","created":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T20:38:05Z","timestamp":1685133485000},"page":"143-149","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Efficient generation of TBX3+ atrioventricular conduction-like cardiomyocytes from human pluripotent stem cells"],"prefix":"10.1016","volume":"669","author":[{"given":"Rulong","family":"Du","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4656-531X","authenticated-orcid":false,"given":"Shuyun","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2023.05.104_bib1","series-title":"Embryonic Tbx3(+) Cardiomyocytes Form the Mature Cardiac Conduction System by Progressive Fate Restriction","first-page":"145","author":"Mohan","year":"2018"},{"key":"10.1016\/j.bbrc.2023.05.104_bib2","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.cardiores.2004.01.030","article-title":"The transcriptional repressor Tbx3 delineates the developing central conduction system of the heart","volume":"62","author":"Hoogaars","year":"2004","journal-title":"Cardiovasc. Res."},{"key":"10.1016\/j.bbrc.2023.05.104_bib3","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1002\/aja.1001970305","article-title":"Expression of homeobox genes Msx-1 (Hox-7) and Msx-2 (Hox-8) during cardiac development in the chick","volume":"197","author":"Chan-Thomas","year":"1993","journal-title":"Dev. Dynam."},{"key":"10.1016\/j.bbrc.2023.05.104_bib4","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s004410100399","article-title":"Overlapping and differential localization of Bmp-2, Bmp-4, Msx-2 and apoptosis in the endocardial cushion and adjacent tissues of the developing mouse heart","volume":"305","author":"Abdelwahid","year":"2001","journal-title":"Cell Tissue Res."},{"key":"10.1016\/j.bbrc.2023.05.104_bib5","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1093\/cvr\/cvn049","article-title":"Msx1 and Msx2 are functional interacting partners of T-box factors in the regulation of Connexin43","volume":"78","author":"Boogerd","year":"2008","journal-title":"Cardiovasc. Res."},{"key":"10.1016\/j.bbrc.2023.05.104_bib6","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/1471-213X-8-75","article-title":"Msx1 and Msx2 are required for endothelial-mesenchymal transformation of the atrioventricular cushions and patterning of the atrioventricular myocardium","volume":"8","author":"Chen","year":"2008","journal-title":"BMC Dev. Biol."},{"key":"10.1016\/j.bbrc.2023.05.104_bib7","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1172\/JCI19846","article-title":"Nkx2-5 mutation causes anatomic hypoplasia of the cardiac conduction system","volume":"113","author":"Jay","year":"2004","journal-title":"J.\u00a0Clin. Invest."},{"key":"10.1016\/j.bbrc.2023.05.104_bib8","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1038\/s41569-018-0031-y","article-title":"Transcriptional regulation of the cardiac conduction system","volume":"15","author":"van Eif","year":"2018","journal-title":"Nat. Rev. Cardiol."},{"key":"10.1016\/j.bbrc.2023.05.104_bib9","doi-asserted-by":"crossref","first-page":"5363","DOI":"10.1242\/dev.00750","article-title":"A\u00a0caudorostral wave of RALDH2 conveys anteroposterior information to the cardiac field","volume":"130","author":"Hochgreb","year":"2003","journal-title":"Development"},{"key":"10.1016\/j.bbrc.2023.05.104_bib10","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1002\/bdra.20804","article-title":"Wnt signaling regulates atrioventricular canal formation upstream of BMP and Tbx2","volume":"91","author":"Verhoeven","year":"2011","journal-title":"Birth Defects Res. A Clin. Mol. Teratol."},{"key":"10.1016\/j.bbrc.2023.05.104_bib11","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1161\/CIRCRESAHA.116.304731","article-title":"Canonical wnt signaling regulates atrioventricular junction programming and electrophysiological properties","volume":"116","author":"Gillers","year":"2015","journal-title":"Circ. Res."},{"key":"10.1016\/j.bbrc.2023.05.104_bib12","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1006\/dbio.2000.9927","article-title":"Expression of chick Tbx-2, Tbx-3, and Tbx-5 genes during early heart development: evidence for BMP2 induction of Tbx2","volume":"228","author":"Yamada","year":"2000","journal-title":"Dev. Biol."},{"key":"10.1016\/j.bbrc.2023.05.104_bib13","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.scr.2017.01.006","article-title":"Chemical-defined and albumin-free generation of human atrial and ventricular myocytes from human pluripotent stem cells","volume":"19","author":"Pei","year":"2017","journal-title":"Stem Cell Res."},{"key":"10.1016\/j.bbrc.2023.05.104_bib14","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1038\/nbt.3745","article-title":"Sinoatrial node cardiomyocytes derived from human pluripotent cells function as a biological pacemaker","volume":"35","author":"Protze","year":"2017","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.bbrc.2023.05.104_bib15","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1038\/cr.2010.163","article-title":"Direct differentiation of atrial and ventricular myocytes from human embryonic stem cells by alternating retinoid signals","volume":"21","author":"Zhang","year":"2011","journal-title":"Cell Res."},{"key":"10.1016\/j.bbrc.2023.05.104_bib16","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1186\/s13287-022-03006-8","article-title":"RA signaling pathway combined with Wnt signaling pathway regulates human-induced pluripotent stem cells (hiPSCs) differentiation to sinus node-like cells","volume":"13","author":"Yin","year":"2022","journal-title":"Stem Cell Res. Ther."},{"key":"10.1016\/j.bbrc.2023.05.104_bib17","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23137318","article-title":"Improved generation of human induced pluripotent stem cell-derived cardiac pacemaker cells using novel differentiation protocols","volume":"23","author":"Darche","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.bbrc.2023.05.104_bib18","doi-asserted-by":"crossref","DOI":"10.1007\/s10565-023-09797-7","article-title":"The method of sinus node-like pacemaker cells from human induced pluripotent stem cells by BMP and Wnt signaling","author":"Wang","year":"2023","journal-title":"Cell Biol. Toxicol."},{"key":"10.1016\/j.bbrc.2023.05.104_bib19","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1177\/2472555220953207","article-title":"Assessment of drug proarrhythmic potential in electrically paced human induced pluripotent stem cell-derived ventricular cardiomyocytes using multielectrode array","volume":"26","author":"Bai","year":"2021","journal-title":"SLAS Discov."},{"key":"10.1016\/j.bbrc.2023.05.104_bib20","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/j.cardiores.2005.06.013","article-title":"Expression and regulation of the atrial natriuretic factor encoding gene Nppa during development and disease","volume":"67","author":"Houweling","year":"2005","journal-title":"Cardiovasc. Res."},{"key":"10.1016\/j.bbrc.2023.05.104_bib21","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1113\/jphysiol.2004.068213","article-title":"Mechanism of spontaneous excitability in human embryonic stem cell derived cardiomyocytes","volume":"559","author":"Satin","year":"2004","journal-title":"J.\u00a0Physiol."},{"key":"10.1016\/j.bbrc.2023.05.104_bib22","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.tcm.2006.05.002","article-title":"Connexin-mediated cardiac impulse propagation: connexin 30.2 slows atrioventricular conduction in mouse heart","volume":"16","author":"Kreuzberg","year":"2006","journal-title":"Trends Cardiovasc. Med."}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X2300685X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X2300685X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T12:30:25Z","timestamp":1757766625000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X2300685X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8]]},"references-count":22,"alternative-id":["S0006291X2300685X"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2023.05.104","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2023,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Efficient generation of TBX3+ atrioventricular conduction-like cardiomyocytes from human pluripotent stem cells","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2023.05.104","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}