{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T21:53:25Z","timestamp":1769118805121,"version":"3.49.0"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,7,13]],"date-time":"2024-07-13T00:00:00Z","timestamp":1720828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2024,10]]},"DOI":"10.1016\/j.bbrc.2024.150400","type":"journal-article","created":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T10:12:48Z","timestamp":1720951968000},"page":"150400","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["A class of chemical compounds enhances clustering of muscle nicotinic acetylcholine receptor in cultured myogenic cells"],"prefix":"10.1016","volume":"731","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1894-8814","authenticated-orcid":false,"given":"Yuichi","family":"Miyairi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bisei","family":"Ohkawara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9991-9118","authenticated-orcid":false,"given":"Ayato","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5355-1204","authenticated-orcid":false,"given":"Ryusuke","family":"Sawada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisao","family":"Ishii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Tomita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taro","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Nakashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1351-7602","authenticated-orcid":false,"given":"Mikako","family":"Ito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6437-2853","authenticated-orcid":false,"given":"Akio","family":"Masuda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuyuki","family":"Hosono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiro","family":"Imagama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1529-2750","authenticated-orcid":false,"given":"Kinji","family":"Ohno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2024.150400_bib1","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.cell.2008.10.002","article-title":"Lrp4 is a receptor for Agrin and forms a complex with MuSK","volume":"135","author":"Kim","year":"2008","journal-title":"Cell"},{"key":"10.1016\/j.bbrc.2024.150400_bib2","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.neuron.2008.10.006","article-title":"LRP4 serves as a coreceptor of agrin","volume":"60","author":"Zhang","year":"2008","journal-title":"Neuron"},{"key":"10.1016\/j.bbrc.2024.150400_bib3","doi-asserted-by":"crossref","first-page":"1802","DOI":"10.1126\/science.1127142","article-title":"The muscle protein Dok-7 is essential for neuromuscular synaptogenesis","volume":"312","author":"Okada","year":"2006","journal-title":"Science"},{"key":"10.1016\/j.bbrc.2024.150400_bib4","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1016\/0955-0674(92)90113-Q","article-title":"Agrin isoforms and their role in synaptogenesis","volume":"4","author":"McMahan","year":"1992","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.bbrc.2024.150400_bib5","doi-asserted-by":"crossref","first-page":"a009167","DOI":"10.1101\/cshperspect.a009167","article-title":"The role of MuSK in synapse formation and neuromuscular disease","volume":"5","author":"Burden","year":"2013","journal-title":"Cold Spring Harbor Perspect. Biol."},{"key":"10.1016\/j.bbrc.2024.150400_bib6","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1080\/14728222.2017.1369960","article-title":"Agrin-LRP4-MuSK signaling as a therapeutic target for myasthenia gravis and other neuromuscular disorders","volume":"21","author":"Ohno","year":"2017","journal-title":"Expert Opin. Ther. Targets"},{"key":"10.1016\/j.bbrc.2024.150400_bib7","doi-asserted-by":"crossref","DOI":"10.1038\/s41582-023-00916-w","article-title":"Myasthenia gravis: the changing treatment landscape in the era of molecular therapies","author":"Iorio","year":"2024","journal-title":"Nat. Rev. Neurol."},{"key":"10.1016\/j.bbrc.2024.150400_bib8","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S1474-4422(21)00463-4","article-title":"Advances and ongoing research in the treatment of autoimmune neuromuscular junction disorders","volume":"21","author":"Verschuuren","year":"2022","journal-title":"Lancet Neurol."},{"key":"10.1016\/j.bbrc.2024.150400_bib9","doi-asserted-by":"crossref","first-page":"2455","DOI":"10.3390\/ijms22052455","article-title":"Secreted signaling molecules at the neuromuscular junction in physiology and pathology","volume":"22","author":"Ohkawara","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.bbrc.2024.150400_bib10","doi-asserted-by":"crossref","first-page":"3730","DOI":"10.3390\/ijms24043730","article-title":"Clinical and pathologic features of congenital myasthenic syndromes caused by 35 genes-A comprehensive review","volume":"24","author":"Ohno","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.bbrc.2024.150400_bib11","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1093\/hmg\/ddl045","article-title":"Aberrant neuromuscular junctions and delayed terminal muscle fiber maturation in alpha-dystroglycanopathies","volume":"15","author":"Taniguchi","year":"2006","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.bbrc.2024.150400_bib12","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1016\/j.neuron.2023.02.028","article-title":"Lost in local translation: TDP-43 and FUS in axonal\/neuromuscular junction maintenance and dysregulation in amyotrophic lateral sclerosis","volume":"111","author":"Piol","year":"2023","journal-title":"Neuron"},{"key":"10.1016\/j.bbrc.2024.150400_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.arr.2023.101966","article-title":"Neuromuscular junction transmission failure in aging and sarcopenia: the nexus of the neurological and muscular systems","volume":"89","author":"Arnold","year":"2023","journal-title":"Ageing Res. Rev."},{"key":"10.1016\/j.bbrc.2024.150400_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.neuropharm.2021.108637","article-title":"Zonisamide upregulates neuregulin-1 expression and enhances acetylcholine receptor clustering at the in vitro neuromuscular junction","volume":"195","author":"Inoue","year":"2021","journal-title":"Neuropharmacology"},{"key":"10.1016\/j.bbrc.2024.150400_bib15","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0142786","article-title":"Zonisamide enhances neurite elongation of primary motor neurons and facilitates peripheral nerve regeneration in vitro and in a mouse model","volume":"10","author":"Yagi","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.bbrc.2024.150400_bib16","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1126\/science.ade2574","article-title":"Evolutionary-scale prediction of atomic-level protein structure with a language model","volume":"379","author":"Lin","year":"2023","journal-title":"Science"},{"key":"10.1016\/j.bbrc.2024.150400_bib17","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1038\/s41467-022-28865-w","article-title":"Improved prediction of protein-protein interactions using AlphaFold2","volume":"13","author":"Bryant","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bbrc.2024.150400_bib18","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1038\/s41586-021-03828-1","article-title":"Highly accurate protein structure prediction for the human proteome","volume":"596","author":"Tunyasuvunakool","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.bbrc.2024.150400_bib19","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/jcc.21334","article-title":"AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading","volume":"31","author":"Trott","year":"2010","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.bbrc.2024.150400_bib20","article-title":"DiffDock: diffusion steps, twists, and turns for molecular docking","author":"Corso","year":"2022","journal-title":"arXiv"},{"key":"10.1016\/j.bbrc.2024.150400_bib21","doi-asserted-by":"crossref","first-page":"1856","DOI":"10.1093\/hmg\/ddt578","article-title":"LRP4 third beta-propeller domain mutations cause novel congenital myasthenia by compromising agrin-mediated MuSK signaling in a position-specific manner","volume":"23","author":"Ohkawara","year":"2014","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.bbrc.2024.150400_bib22","doi-asserted-by":"crossref","DOI":"10.1038\/srep28512","article-title":"R-spondin 2 promotes acetylcholine receptor clustering at the neuromuscular junction via Lgr5","volume":"6","author":"Nakashima","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bbrc.2024.150400_bib23","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1111\/j.1600-0773.1984.tb01992.x","article-title":"Neurotoxic effects of disulfiram on autonomic nervous system in rat","volume":"55","author":"Savolainen","year":"1984","journal-title":"Acta Pharmacol. Toxicol."},{"key":"10.1016\/j.bbrc.2024.150400_bib24","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1016\/j.cell.2011.02.037","article-title":"SnapShot: neuromuscular junction","volume":"144","author":"Burden","year":"2011","journal-title":"Cell"},{"key":"10.1016\/j.bbrc.2024.150400_bib25","doi-asserted-by":"crossref","first-page":"32370","DOI":"10.1074\/jbc.M110.144774","article-title":"Modulation of agrin-induced acetylcholine receptor clustering by extracellular signal-regulated kinases 1 and 2 in cultured myotubes","volume":"285","author":"Rimer","year":"2010","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2024.150400_bib26","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1002\/neu.10197","article-title":"MAPK signal transduction pathway mediates agrin effects on neurite elongation in cultured hippocampal neurons","volume":"55","author":"Karasewski","year":"2003","journal-title":"J. Neurobiol."},{"key":"10.1016\/j.bbrc.2024.150400_bib27","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/0092-8674(90)90252-A","article-title":"Deletions and a translocation interrupt a cloned gene at the neurofibromatosis type 1 locus","volume":"62","author":"Viskochil","year":"1990","journal-title":"Cell"},{"key":"10.1016\/j.bbrc.2024.150400_bib28","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1038\/ng0296-144","article-title":"Loss of NF1 results in activation of the Ras signaling pathway and leads to aberrant growth in haematopoietic cells","volume":"12","author":"Bollag","year":"1996","journal-title":"Nat. Genet."},{"key":"10.1016\/j.bbrc.2024.150400_bib29","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S0165-3806(01)00190-0","article-title":"Differential localization of the neurofibromatosis 1 (NF1) gene product, neurofibromin, with the F-actin or microtubule cytoskeleton during differentiation of telencephalic neurons","volume":"130","author":"Li","year":"2001","journal-title":"Brain Res. Dev. Brain Res."},{"key":"10.1016\/j.bbrc.2024.150400_bib30","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1126\/science.276.5313.795","article-title":"Requirement of Drosophila NF1 for activation of adenylyl cyclase by PACAP38-like neuropeptides","volume":"276","author":"Guo","year":"1997","journal-title":"Science"},{"key":"10.1016\/j.bbrc.2024.150400_bib31","doi-asserted-by":"crossref","first-page":"16971","DOI":"10.1523\/JNEUROSCI.1756-12.2012","article-title":"Neurofibromin mediates FAK signaling in confining synapse growth at Drosophila neuromuscular junctions","volume":"32","author":"Tsai","year":"2012","journal-title":"J. Neurosci."},{"key":"10.1016\/j.bbrc.2024.150400_bib32","doi-asserted-by":"crossref","first-page":"9450","DOI":"10.1523\/JNEUROSCI.0562-22.2022","article-title":"Loss of NF1 in Drosophila larvae causes tactile hypersensitivity and impaired synaptic transmission at the neuromuscular junction","volume":"42","author":"Dyson","year":"2022","journal-title":"J. Neurosci."},{"key":"10.1016\/j.bbrc.2024.150400_bib33","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1534\/g3.118.200795","article-title":"Conditional inactivation of Nf1 and pten in Schwann cells results in abnormal neuromuscular junction maturation","volume":"9","author":"Li","year":"2019","journal-title":"G3 (Bethesda)"},{"key":"10.1016\/j.bbrc.2024.150400_bib34","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1038\/ng772","article-title":"Mutations in PTPN11, encoding the protein tyrosine phosphatase SHP-2, cause Noonan syndrome","volume":"29","author":"Tartaglia","year":"2001","journal-title":"Nat. Genet."},{"key":"10.1016\/j.bbrc.2024.150400_bib35","article-title":"Mutations in PTPN11 could lead to a congenital myasthenic syndrome phenotype: a Noonan syndrome case series","author":"Pugliese","year":"2023","journal-title":"J. Neurol."},{"key":"10.1016\/j.bbrc.2024.150400_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/j.neulet.2019.134671","article-title":"Extracellular signal-regulated kinases 1 and 2 regulate neuromuscular junction and myofiber phenotypes in mammalian skeletal muscle","volume":"715","author":"Rimer","year":"2020","journal-title":"Neurosci. Lett."},{"key":"10.1016\/j.bbrc.2024.150400_bib37","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1002\/dvdy.20322","article-title":"Cullin-5 plays multiple roles in cell fate specification and synapse formation during Drosophila development","volume":"232","author":"Ayyub","year":"2005","journal-title":"Dev. Dynam."},{"key":"10.1016\/j.bbrc.2024.150400_bib38","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1007\/s12041-011-0062-1","article-title":"Cullin-5 and cullin-2 play a role in the development of neuromuscular junction and the female germ line of Drosophila","volume":"90","author":"Ayyub","year":"2011","journal-title":"J. Genet."}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X24009367?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X24009367?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,11,6]],"date-time":"2024-11-06T04:42:04Z","timestamp":1730868124000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X24009367"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10]]},"references-count":38,"alternative-id":["S0006291X24009367"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2024.150400","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2024,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A class of chemical compounds enhances clustering of muscle nicotinic acetylcholine receptor in cultured myogenic cells","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2024.150400","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"150400"}}