{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T15:48:38Z","timestamp":1777477718803,"version":"3.51.4"},"reference-count":32,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100018925","name":"Health Commission of Henan Province","doi-asserted-by":"publisher","award":["SBGJ202103097"],"award-info":[{"award-number":["SBGJ202103097"]}],"id":[{"id":"10.13039\/100018925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81900843"],"award-info":[{"award-number":["81900843"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81900889"],"award-info":[{"award-number":["81900889"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570825"],"award-info":[{"award-number":["81570825"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070585"],"award-info":[{"award-number":["32070585"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U1604171"],"award-info":[{"award-number":["U1604171"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017700","name":"Henan Provincial Science and Technology Research Project","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100017700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2025,1]]},"DOI":"10.1016\/j.bbrc.2024.151194","type":"journal-article","created":{"date-parts":[[2024,12,14]],"date-time":"2024-12-14T22:18:38Z","timestamp":1734214718000},"page":"151194","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Coptisine acts as a nucleolus fluorescent probe in vitro"],"prefix":"10.1016","volume":"744","author":[{"given":"Hui","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuangshuang","family":"An","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiukun","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingli","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengling","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weikai","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanzhong","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2024.151194_bib1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s13020-018-0171-3","article-title":"Coptidis rhizoma and its main bioactive components: recent advances in chemical investigation, quality evaluation and pharmacological activity","volume":"13","author":"Meng","year":"2018","journal-title":"Chin. Med."},{"key":"10.1016\/j.bbrc.2024.151194_bib2","doi-asserted-by":"crossref","first-page":"281","DOI":"10.4149\/gpb_2019014","article-title":"Activation of the Nrf2\/HO-1 signaling pathway contributes to the protective e\ufb00ects of coptisine against oxidative stress-induced DNA damage and apoptosis in HaCaT keratinocytes","volume":"38","author":"Choi","year":"2019","journal-title":"Gen. Physiol. Biophys."},{"key":"10.1016\/j.bbrc.2024.151194_bib3","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/s00210-019-01710-6","article-title":"Coptisine ameliorates renal injury in diabetic rats through the activation of Nrf2 signaling pathway","volume":"393","author":"Zhai","year":"2020","journal-title":"Naunyn-Schmiedeberg\u2019s Arch. Pharmacol."},{"key":"10.1016\/j.bbrc.2024.151194_bib4","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1016\/j.biopha.2018.04.052","article-title":"Coptisine from Rhizoma coptidis exerts an anti-cancer effect on hepatocellular carcinoma by up-regulating miR-122","volume":"103","author":"Chai","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"10.1016\/j.bbrc.2024.151194_bib5","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.phymed.2017.12.027","article-title":"Coptisine\u2010induced apoptosis in human colon cancer cells (HCT\u2010116) is mediated by PI3K\/Akt and mitochondrial\u2010associated apoptotic pathway","volume":"48","author":"Han","year":"2018","journal-title":"Phytomedicine"},{"key":"10.1016\/j.bbrc.2024.151194_bib6","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1016\/j.jbbm.2007.07.009","article-title":"Antimicrobial properties of berberines alkaloids in Coptis chinensis Franch by microcalorimetry","volume":"70","author":"Yan","year":"2008","journal-title":"J. Biochem. Biophys. Methods"},{"key":"10.1016\/j.bbrc.2024.151194_bib7","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.ejphar.2017.06.027","article-title":"Anti-inflammatory activity of coptisine free base in mice through inhibition of NF-kappaB and MAPK signaling pathways","volume":"811","author":"Chen","year":"2017","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.bbrc.2024.151194_bib8","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.ejphar.2016.03.037","article-title":"Coptisine from Coptis chinensis inhib\u2010its production of inflammatory mediators in lipopolysaccharide\u2010stimulated RAW 264.7 murine macrophage cells","volume":"780","author":"Wu","year":"2016","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.bbrc.2024.151194_bib9","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1248\/bpb.32.1433","article-title":"Anti alzheimer and antioxidant activities of coptidis rhizoma alkaloids","volume":"32","author":"Jung","year":"2009","journal-title":"Biol. Pharm. Bull."},{"key":"10.1016\/j.bbrc.2024.151194_bib10","article-title":"Neuroprotective activity of coptisine from Coptis chinensis (Franch)","volume":"2015","author":"Friedemann","year":"2015","journal-title":"Evid. Based Compleent Alternat. Med."},{"key":"10.1016\/j.bbrc.2024.151194_bib11","doi-asserted-by":"crossref","first-page":"13183","DOI":"10.1021\/acs.analchem.8b04070","article-title":"Supramolecularly multicolor DNA decoding using an indicator competition assay","volume":"90","author":"Zhou","year":"2018","journal-title":"Anal. Chem."},{"key":"10.1016\/j.bbrc.2024.151194_bib12","doi-asserted-by":"crossref","first-page":"3128","DOI":"10.1021\/acs.jnatprod.7b00350","article-title":"Role of the benzodioxole group in the interactions between the natural alkaloids chelerythrine and coptisine and the human telomeric G-quadruplex DNA. A multiapproach investigation","volume":"80","author":"Papi","year":"2017","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.bbrc.2024.151194_bib13","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.1016\/j.talanta.2009.10.049","article-title":"Supramolecular interaction of cucurbit[n]urils and coptisine by spectrofluorimetry and its analytical application","volume":"80","author":"Li","year":"2010","journal-title":"Talanta"},{"key":"10.1016\/j.bbrc.2024.151194_bib14","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/j.talanta.2014.09.005","article-title":"A competitive strategy based on cucurbit[7]uril supramolecular interaction for simple and sensitive detection of dibucaine","volume":"132","author":"Li","year":"2015","journal-title":"Talanta"},{"key":"10.1016\/j.bbrc.2024.151194_bib15","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1038\/nrclinonc.2017.166","article-title":"Tumour heterogeneity and resistance to cancer therapies","volume":"15","author":"Ibiayi","year":"2018","journal-title":"Nat. Rev. Clin. Oncol."},{"key":"10.1016\/j.bbrc.2024.151194_bib16","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/978-3-030-14366-4_4","article-title":"Heterogeneity of hepatic cancer stem cells","volume":"1139","author":"Sukowati","year":"2019","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.bbrc.2024.151194_bib30","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1002\/path.2283","article-title":"CD133: molecule of the moment","volume":"214","author":"Mizrak","year":"2008","journal-title":"J. Pathol."},{"key":"10.1016\/j.bbrc.2024.151194_bib31","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1016\/j.cell.2018.10.008","article-title":"T helper cell cytokines modulate intestinal stem cell renewal and differentiation","volume":"175","author":"Biton","year":"2018","journal-title":"Cell"},{"key":"10.1016\/j.bbrc.2024.151194_bib17","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1038\/nrm2184","article-title":"The multifunctional nucleolus","volume":"8","author":"Boisvert","year":"2007","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.bbrc.2024.151194_bib18","doi-asserted-by":"crossref","first-page":"3871","DOI":"10.1093\/nar\/26.17.3871","article-title":"The plurifunctional nucleolus","volume":"26","author":"Pederson","year":"1998","journal-title":"Nucleic Acids Res."},{"issue":"5","key":"10.1016\/j.bbrc.2024.151194_bib19","first-page":"1291","article-title":"Nucleolar function and size in cancer cells","volume":"152","author":"Derenzini","year":"1998","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.bbrc.2024.151194_bib20","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1074\/mcp.M900338-MCP200","article-title":"Proteomics analysis of the nucleolus in adenovirus-infected cells","volume":"9","author":"Lam","year":"2010","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.bbrc.2024.151194_bib21","first-page":"48","article-title":"New insights into nucleolar structure and function","volume":"7","author":"Lam","year":"2015","journal-title":"F1000 Prim. Rep."},{"key":"10.1016\/j.bbrc.2024.151194_bib22","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/S0074-7696(06)55004-1","article-title":"New insights into nucleolar architecture and activity","volume":"255","author":"Raska","year":"2006","journal-title":"Int. Rev. Cytol."},{"key":"10.1016\/j.bbrc.2024.151194_bib23","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1007\/s00109-012-0981-1","article-title":"Nucleolar activity in neurodegenerative diseases: a missing piece of the puzzle?","volume":"91","author":"Parlato","year":"2013","journal-title":"J. Mol. Med."},{"key":"10.1016\/j.bbrc.2024.151194_bib24","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/embor.2008.230","article-title":"Old and new faces of the nucleolus. Workshop on the Nucleolus and Disease","volume":"10","author":"Stark","year":"2009","journal-title":"EMBO Rep."},{"key":"10.1016\/j.bbrc.2024.151194_bib25","doi-asserted-by":"crossref","DOI":"10.1002\/adbi.201900047","article-title":"Green fluorescent protein nanovessel serves as a nucleolus targeting material and molecule carrier in living cells","volume":"3","author":"Li","year":"2019","journal-title":"Adv. Biosyst."},{"key":"10.1016\/j.bbrc.2024.151194_bib26","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1038\/embor.2012.61","article-title":"Cancer chromosomal instability: therapeutic and diagnostic challenges","volume":"13","author":"Mcgranahan","year":"2012","journal-title":"EMBO Rep."},{"key":"10.1016\/j.bbrc.2024.151194_bib27","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pmed.1001961","article-title":"Microenvironmental heterogeneity parallels breast cancer progression: a histology-genomic integration analysis","volume":"13","author":"Natrajan","year":"2016","journal-title":"PLoS Med."},{"key":"10.1016\/j.bbrc.2024.151194_bib28","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1038\/nrd4253","article-title":"Tackling the cancer stem cells - what challenges do they pose?","volume":"13","author":"Pattabiraman","year":"2014","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.bbrc.2024.151194_bib29","doi-asserted-by":"crossref","first-page":"34936","DOI":"10.1074\/jbc.C600138200","article-title":"Hyaluronan constitutively regulates activation of multiple receptor tyrosine kinases in epithelial and carcinoma cells","volume":"281","author":"Misra","year":"2006","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2024.151194_bib32","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/S0091-679X(08)00014-9","article-title":"Isolation and culture of colon cancer stem cells","volume":"86","author":"Cammareri","year":"2008","journal-title":"Methods Cell Biol."}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X24017303?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X24017303?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T13:59:33Z","timestamp":1777384773000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X24017303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":32,"alternative-id":["S0006291X24017303"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2024.151194","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Coptisine acts as a nucleolus fluorescent probe in vitro","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2024.151194","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"151194"}}