{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T17:39:15Z","timestamp":1772645955668,"version":"3.50.1"},"reference-count":50,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2025,4]]},"DOI":"10.1016\/j.bbrc.2025.151619","type":"journal-article","created":{"date-parts":[[2025,3,13]],"date-time":"2025-03-13T19:30:28Z","timestamp":1741894228000},"page":"151619","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["The experimental study of the effect of fluid shear force on the migration rate of human umbilical vein endothelial cells"],"prefix":"10.1016","volume":"758","author":[{"given":"Dong","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianwei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaming","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liding","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2025.151619_bib1","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1016\/j.molmed.2017.07.007","article-title":"Molecular sensors of blood flow in endothelial cells","volume":"23","author":"Baratchi","year":"2017","journal-title":"Trends Mol. Med."},{"key":"10.1016\/j.bbrc.2025.151619_bib2","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1161\/CIRCRESAHA.107.152942","article-title":"Biomechanical forces in atherosclerosis-resistant vascular regions regulate endothelial redox balance via phosphoinositol 3-kinase\/Akt-dependent activation of Nrf2","volume":"101","author":"Dai","year":"2007","journal-title":"Circ. Res."},{"key":"10.1016\/j.bbrc.2025.151619_bib3","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1038\/362801a0","article-title":"The pathogenesis of atherosclerosis: a perspective for the 1990s","volume":"362","author":"Ross","year":"1993","journal-title":"Nature"},{"key":"10.1016\/j.bbrc.2025.151619_bib4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.gene.2018.04.029","article-title":"miR-758 mediates oxLDL-dependent vascular endothelial cell damage by suppressing the succinate receptor SUCNR1","volume":"663","author":"Zhang","year":"2018","journal-title":"Gene"},{"key":"10.1016\/j.bbrc.2025.151619_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2023.122311","article-title":"Gas station in blood vessels: an endothelium mimicking, self-sustainable nitric oxide fueling stent coating for prevention of thrombosis and restenosis","volume":"302","author":"Rao","year":"2023","journal-title":"Biomaterials"},{"key":"10.1016\/j.bbrc.2025.151619_bib6","doi-asserted-by":"crossref","DOI":"10.3390\/biomedicines11113051","article-title":"The impact of modern anti-diabetic treatment on endothelial progenitor cells","volume":"11","author":"Altabas","year":"2023","journal-title":"Biomedicines"},{"key":"10.1016\/j.bbrc.2025.151619_bib7","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1161\/01.ATV.0000073832.49290.B5","article-title":"Endothelial progenitor cells: mobilization, differentiation, and homing","volume":"23","author":"Hristov","year":"2003","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"10.1016\/j.bbrc.2025.151619_bib8","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adj8540","article-title":"Flow in fetoplacental-like microvessels in vitro enhances perfusion, barrier function, and matrix stability","volume":"9","author":"Cherubini","year":"2023","journal-title":"Sci. Adv."},{"key":"10.1016\/j.bbrc.2025.151619_bib9","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1152\/physrev.00047.2009","article-title":"Effects of disturbed flow on vascular endothelium: pathophysiological basis and clinical perspectives","volume":"91","author":"Chiu","year":"2011","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.bbrc.2025.151619_bib10","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.biotechadv.2013.11.007","article-title":"Engineering cell alignment in vitro","volume":"32","author":"Li","year":"2014","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.bbrc.2025.151619_bib11","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1002\/jbm.a.34059","article-title":"Endothelial cell responses to micropillar substrates of varying dimensions and stiffness","volume":"100","author":"Dickinson","year":"2012","journal-title":"J. Biomed. Mater. Res."},{"key":"10.1016\/j.bbrc.2025.151619_bib12","doi-asserted-by":"crossref","first-page":"2928","DOI":"10.1016\/j.biomaterials.2013.01.017","article-title":"Spatial patterning of endothelium modulates cell morphology, adhesiveness and transcriptional signature","volume":"34","author":"Huang","year":"2013","journal-title":"Biomaterials"},{"key":"10.1016\/j.bbrc.2025.151619_bib13","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.actbio.2007.07.008","article-title":"Improved endothelial cell adhesion and proliferation on patterned titanium surfaces with rationally designed, micrometer to nanometer features","volume":"4","author":"Lu","year":"2008","journal-title":"Acta Biomater."},{"key":"10.1016\/j.bbrc.2025.151619_bib14","doi-asserted-by":"crossref","first-page":"C657","DOI":"10.1152\/ajpcell.00363.2014","article-title":"Fluid shear, intercellular stress, and endothelial cell alignment","volume":"308","author":"Steward","year":"2015","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"10.1016\/j.bbrc.2025.151619_bib15","doi-asserted-by":"crossref","first-page":"2130","DOI":"10.1161\/ATVBAHA.113.301826","article-title":"Endothelial cell sensing of flow direction","volume":"33","author":"Wang","year":"2013","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"10.1016\/j.bbrc.2025.151619_bib16","doi-asserted-by":"crossref","first-page":"8356","DOI":"10.1038\/s41467-023-43875-y","article-title":"Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles","volume":"14","author":"Nanda","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bbrc.2025.151619_bib17","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1007\/s00011-023-01761-9","article-title":"Role of RhoG as a regulator of cellular functions: integrating insights on immune cell activation, migration, and functions","volume":"72","author":"Rai","year":"2023","journal-title":"Inflamm. Res."},{"key":"10.1016\/j.bbrc.2025.151619_bib18","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1002\/bit.260320812","article-title":"Shear stress induced stimulation of mammalian cell metabolism","volume":"32","author":"Frangos","year":"1988","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.bbrc.2025.151619_bib19","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1007\/s10439-005-1735-9","article-title":"Non-uniform flow behavior in a parallel plate flow chamber : alters endothelial cell responses","volume":"33","author":"McCann","year":"2005","journal-title":"Ann. Biomed. Eng."},{"key":"10.1016\/j.bbrc.2025.151619_bib20","first-page":"5387","article-title":"Investigating the effects of fluid shear forces on cellular responses to profiled surfaces in-vitro: a computational and experimental investigation","volume":"2007","author":"Brown","year":"2007","journal-title":"Annu Int Conf IEEE Eng Med Biol Soc"},{"key":"10.1016\/j.bbrc.2025.151619_bib21","doi-asserted-by":"crossref","first-page":"W5","DOI":"10.1093\/nar\/gkn201","article-title":"NCBI BLAST: a better web interface","volume":"36","author":"Johnson","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.bbrc.2025.151619_bib22","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1007\/s00299-004-0859-y","article-title":"Use of real-time PCR for determining copy number and zygosity in transgenic plants","volume":"23","author":"Bubner","year":"2004","journal-title":"Plant Cell Rep."},{"key":"10.1016\/j.bbrc.2025.151619_bib23","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.yjmcc.2021.10.009","article-title":"Endothelial repair by stem and progenitor cells","volume":"163","author":"Wang","year":"2022","journal-title":"J. Mol. Cell. Cardiol."},{"key":"10.1016\/j.bbrc.2025.151619_bib24","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1163\/156856208786052353","article-title":"Fibrinogen and von Willebrand factor mediated platelet adhesion to polystyrene under flow conditions","volume":"19","author":"Zhang","year":"2008","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"10.1016\/j.bbrc.2025.151619_bib25","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1161\/01.ATV.6.2.146","article-title":"Injury and repair of endothelium at sites of flow disturbances near abdominal aortic coarctations in rabbits","volume":"6","author":"Langille","year":"1986","journal-title":"Arteriosclerosis"},{"key":"10.1016\/j.bbrc.2025.151619_bib26","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/978-1-4615-5959-7_13","article-title":"Endothelial gene regulation by laminar shear stress","volume":"430","author":"Resnick","year":"1997","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.bbrc.2025.151619_bib27","doi-asserted-by":"crossref","first-page":"4131","DOI":"10.1007\/s00018-014-1678-0","article-title":"Mechanisms of endothelial cell migration","volume":"71","author":"Michaelis","year":"2014","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.bbrc.2025.151619_bib28","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1385\/CBB:38:3:323","article-title":"Differential responsiveness of vascular endothelial cells to different types of fluid mechanical shear stress","volume":"38","author":"Barakat","year":"2003","journal-title":"Cell Biochem. Biophys."},{"key":"10.1016\/j.bbrc.2025.151619_bib29","doi-asserted-by":"crossref","first-page":"4478","DOI":"10.1073\/pnas.071052598","article-title":"Biomechanical activation of vascular endothelium as a determinant of its functional phenotype","volume":"98","author":"Garcia-Cardena","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.bbrc.2025.151619_bib30","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1152\/physrev.1995.75.3.519","article-title":"Flow-mediated endothelial mechanotransduction","volume":"75","author":"Davies","year":"1995","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.bbrc.2025.151619_bib31","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1111\/j.1749-6632.1994.tb17310.x","article-title":"Hemodynamics and atherogenesis. Endothelial surface dynamics in flow signal transduction","volume":"748","author":"Davies","year":"1995","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.bbrc.2025.151619_bib32","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1007\/s10237-013-0502-3","article-title":"Shear stress-induced redistribution of the glycocalyx on endothelial cells in vitro","volume":"13","author":"Bai","year":"2014","journal-title":"Biomech. Model. Mechanobiol."},{"key":"10.1016\/j.bbrc.2025.151619_bib33","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1089\/ars.2013.5507","article-title":"Hemodynamic regulation of reactive oxygen species: implications for vascular diseases","volume":"20","author":"Raaz","year":"2014","journal-title":"Antioxidants Redox Signal."},{"key":"10.1016\/j.bbrc.2025.151619_bib34","first-page":"2107","article-title":"Decreased blood flow rate disrupts endothelial repair in vivo","volume":"149","author":"Vyalov","year":"1996","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.bbrc.2025.151619_bib35","doi-asserted-by":"crossref","first-page":"H293","DOI":"10.1152\/ajpheart.2000.279.1.H293","article-title":"Shear stress enhances human endothelial cell wound closure in vitro","volume":"279","author":"Albuquerque","year":"2000","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"10.1016\/j.bbrc.2025.151619_bib36","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1161\/hq0102.101518","article-title":"Shear stress-induced endothelial cell migration involves integrin signaling via the fibronectin receptor subunits alpha(5) and beta(1)","volume":"22","author":"Urbich","year":"2002","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"10.1016\/j.bbrc.2025.151619_bib37","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/nrcardio.2013.13","article-title":"New concepts in the design of drug-eluting coronary stents","volume":"10","author":"Garg","year":"2013","journal-title":"Nat. Rev. Cardiol."},{"key":"10.1016\/j.bbrc.2025.151619_bib38","first-page":"485","article-title":"Identification of endothelial protein C receptor as a novel druggable agonistic target for reendothelialization promotion and thrombosis prevention of eluting stent","volume":"41","author":"Chen","year":"2024","journal-title":"Bioact. Mater."},{"key":"10.1016\/j.bbrc.2025.151619_bib39","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1161\/CIRCULATIONAHA.122.061516","article-title":"Peptide vaccine against ADAMTS-7 ameliorates atherosclerosis and postinjury neointima hyperplasia","volume":"147","author":"Ma","year":"2023","journal-title":"Circulation"},{"key":"10.1016\/j.bbrc.2025.151619_bib40","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1186\/s12938-016-0263-1","article-title":"beta1 integrin signaling in asymmetric migration of keratinocytes under mechanical stretch in a co-cultured wound repair model","volume":"15","author":"Lu","year":"2016","journal-title":"Biomed. Eng. Online"},{"key":"10.1016\/j.bbrc.2025.151619_bib41","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1177\/153537020222701109","article-title":"Wound closure in sheared endothelial cells is enhanced by modulation of vascular endothelial-cadherin expression and localization","volume":"227","author":"Albuquerque","year":"2002","journal-title":"Exp. Biol. Med."},{"key":"10.1016\/j.bbrc.2025.151619_bib42","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1038\/nrm.2016.67","article-title":"Regulating Rho GTPases and their regulators","volume":"17","author":"Hodge","year":"2016","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.bbrc.2025.151619_bib43","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/s41580-023-00682-z","article-title":"Patterning of the cell cortex by Rho GTPases","volume":"25","author":"Bement","year":"2024","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.bbrc.2025.151619_bib44","doi-asserted-by":"crossref","DOI":"10.1016\/j.neuint.2024.105883","article-title":"Ras, RhoA, and vascular pharmacology in neurodevelopment and aging","volume":"181","author":"Nussinov","year":"2024","journal-title":"Neurochem. Int."},{"key":"10.1016\/j.bbrc.2025.151619_bib45","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1006\/bbrc.2001.5221","article-title":"Effects of flow patterns on endothelial cell migration into a zone of mechanical denudation","volume":"285","author":"Hsu","year":"2001","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2025.151619_bib46","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.jmbbm.2017.04.028","article-title":"Combination of flow and micropattern alignment affecting flow-resistant endothelial cell adhesion","volume":"74","author":"Gong","year":"2017","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"10.1016\/j.bbrc.2025.151619_bib47","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1186\/s13019-019-1025-5","article-title":"High shear stress suppresses proliferation and migration but promotes apoptosis of endothelial cells co-cultured with vascular smooth muscle cells via down-regulating MAPK pathway","volume":"14","author":"Ji","year":"2019","journal-title":"J. Cardiothorac. Surg."},{"key":"10.1016\/j.bbrc.2025.151619_bib48","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1007\/s12195-013-0275-x","article-title":"High-level shear stress stimulates endothelial differentiation and VEGF secretion by human mesenchymal stem cells","volume":"6","author":"Yuan","year":"2013","journal-title":"Cell. Mol. Bioeng."},{"key":"10.1016\/j.bbrc.2025.151619_bib49","doi-asserted-by":"crossref","first-page":"237","DOI":"10.21037\/jgo-23-844","article-title":"Activation of RhoA\/ROCK2 signaling by hypoxia-inducible factor 1alpha in promoting tumor growth and metastasis in human colon cancer","volume":"15","author":"Du","year":"2024","journal-title":"J. Gastrointest. Oncol."},{"key":"10.1016\/j.bbrc.2025.151619_bib50","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1038\/s41421-024-00696-7","article-title":"RhoA-mediated G12-G13 signaling maintains muscle stem cell quiescence and prevents stem cell loss","volume":"10","author":"Peng","year":"2024","journal-title":"Cell Discovery"}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X2500333X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X2500333X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,4,6]],"date-time":"2025-04-06T08:46:28Z","timestamp":1743929188000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X2500333X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4]]},"references-count":50,"alternative-id":["S0006291X2500333X"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2025.151619","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2025,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The experimental study of the effect of fluid shear force on the migration rate of human umbilical vein endothelial cells","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2025.151619","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"151619"}}