{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T00:38:35Z","timestamp":1793234315985,"version":"4.5.0"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"am","delay-in-days":330,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["R01AA028859"],"award-info":[{"award-number":["R01AA028859"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1016\/j.bbrc.2025.151900","type":"journal-article","created":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T15:45:01Z","timestamp":1745595901000},"page":"151900","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Gene-dose effect of the glutathione biosynthesis gene on ascorbate deficiency in mice"],"prefix":"10.1016","volume":"766","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-7149-930X","authenticated-orcid":false,"given":"Reagan","family":"Strand","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0417-1400","authenticated-orcid":false,"given":"David J.","family":"Orlicky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2025.151900_bib1","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/bs.vh.2022.09.002","article-title":"The antioxidant glutathione","volume":"121","author":"Averill-Bates","year":"2023","journal-title":"Vitam Horm"},{"key":"10.1016\/j.bbrc.2025.151900_bib2","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1016\/j.freeradbiomed.2004.06.040","article-title":"Genetically altered mice to evaluate glutathione homeostasis in health and disease","volume":"37","author":"Dalton","year":"2004","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.bbrc.2025.151900_bib3","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.fct.2013.07.008","article-title":"Glutathione defense mechanism in liver injury: insights from animal models","volume":"60","author":"Chen","year":"2013","journal-title":"Food Chem. Toxicol."},{"key":"10.1016\/j.bbrc.2025.151900_bib4","doi-asserted-by":"crossref","first-page":"49446","DOI":"10.1074\/jbc.M209372200","article-title":"Initial characterization of the glutamate-cysteine ligase modifier subunit Gclm(-\/-) knockout mouse. Novel model system for a severely compromised oxidative stress response","volume":"277","author":"Yang","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2025.151900_bib5","doi-asserted-by":"crossref","first-page":"33766","DOI":"10.1074\/jbc.M504604200","article-title":"Glutamate cysteine ligase catalysis: dependence on ATP and modifier subunit for regulation of tissue glutathione levels","volume":"280","author":"Chen","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2025.151900_bib6","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.freeradbiomed.2020.07.013","article-title":"Ascorbic acid: the chemistry underlying its antioxidant properties","volume":"159","author":"Njus","year":"2020","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.bbrc.2025.151900_bib7","doi-asserted-by":"crossref","first-page":"1203S","DOI":"10.1093\/ajcn\/54.6.1203s","article-title":"Molecular basis for the deficiency in humans of gulonolactone oxidase, a key enzyme for ascorbic acid biosynthesis","volume":"54","author":"Nishikimi","year":"1991","journal-title":"Am. J. Clin. Nutr."},{"key":"10.1016\/j.bbrc.2025.151900_bib8","doi-asserted-by":"crossref","DOI":"10.3390\/nu12072008","article-title":"Global vitamin C status and prevalence of deficiency: a cause for concern","volume":"12","author":"Rowe","year":"2020","journal-title":"Nutrients"},{"key":"10.1016\/j.bbrc.2025.151900_bib9","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1007\/BF03174092","article-title":"Vitamin C transporters","volume":"64","author":"Rivas","year":"2008","journal-title":"J. Physiol. Biochem."},{"key":"10.1016\/j.bbrc.2025.151900_bib10","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1146\/annurev.nutr.25.050304.092647","article-title":"Regulation of vitamin C transport","volume":"25","author":"Wilson","year":"2005","journal-title":"Annu. Rev. Nutr."},{"issue":"1","key":"10.1016\/j.bbrc.2025.151900_bib11","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1177\/1533210110392951","article-title":"Vitamin C: overview and update","volume":"16","author":"Schlueter","year":"2011","journal-title":"Journal of Evidence-Based Complementary & Alternative Medicine"},{"key":"10.1016\/j.bbrc.2025.151900_bib12","series-title":"Vitamin C Deficiency, StatPearls","author":"Maxfield","year":"2025"},{"key":"10.1016\/j.bbrc.2025.151900_bib13","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1073\/pnas.97.2.841","article-title":"Aortic wall damage in mice unable to synthesize ascorbic acid","volume":"97","author":"Maeda","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.bbrc.2025.151900_bib14","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1111\/j.1601-183X.2011.00762.x","article-title":"Effect of vitamin C deficiency during postnatal development on adult behavior: functional phenotype of Gulo-\/- knockout mice","volume":"11","author":"Chen","year":"2012","journal-title":"Genes Brain Behav"},{"key":"10.1016\/j.bbrc.2025.151900_bib15","doi-asserted-by":"crossref","first-page":"1905","DOI":"10.1016\/0006-2952(92)90091-V","article-title":"On the antioxidant effects of ascorbic acid and glutathione","volume":"44","author":"Meister","year":"1992","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.bbrc.2025.151900_bib16","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1093\/ajcn\/71.5.1194","article-title":"Direct correlation of glutathione and ascorbate and their dependence on age and season in human lymphocytes","volume":"71","author":"Lenton","year":"2000","journal-title":"Am. J. Clin. Nutr."},{"key":"10.1016\/j.bbrc.2025.151900_bib17","doi-asserted-by":"crossref","DOI":"10.3390\/antiox12020448","article-title":"Fatal epileptic seizures in mice having compromised glutathione and ascorbic acid biosynthesis","volume":"12","author":"Chen","year":"2023","journal-title":"Antioxidants"},{"key":"10.1016\/j.bbrc.2025.151900_bib18","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1006\/abio.2000.4498","article-title":"Determining glutathione and glutathione disulfide using the fluorescence probe o-phthalaldehyde","volume":"280","author":"Senft","year":"2000","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.bbrc.2025.151900_bib19","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/0006-2944(74)90093-3","article-title":"A rapid micromethod for the determination of ascorbic acid in plasma and tissues","volume":"11","author":"Zannoni","year":"1974","journal-title":"Biochem. Med."},{"key":"10.1016\/j.bbrc.2025.151900_bib20","first-page":"96","article-title":"Practical murine hematopathology: a comparative review and implications for research","volume":"65","author":"O'Connell","year":"2015","journal-title":"Comp. Med."},{"key":"10.1016\/j.bbrc.2025.151900_bib21","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.mam.2008.08.009","article-title":"Structure, function, and post-translational regulation of the catalytic and modifier subunits of glutamate cysteine ligase","volume":"30","author":"Franklin","year":"2009","journal-title":"Mol Aspects Med"},{"key":"10.1016\/j.bbrc.2025.151900_bib22","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1016\/j.freeradbiomed.2004.06.011","article-title":"Human glutamate cysteine ligase gene regulation through the electrophile response element","volume":"37","author":"Dickinson","year":"2004","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.bbrc.2025.151900_bib23","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1093\/toxsci\/kfm165","article-title":"Glutamate cysteine ligase modifier subunit deficiency and gender as determinants of acetaminophen-induced hepatotoxicity in mice","volume":"99","author":"McConnachie","year":"2007","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.bbrc.2025.151900_bib24","doi-asserted-by":"crossref","first-page":"3818","DOI":"10.3390\/nu6093818","article-title":"Does vitamin C deficiency affect cognitive development and function?","volume":"6","author":"Hansen","year":"2014","journal-title":"Nutrients"},{"key":"10.1016\/j.bbrc.2025.151900_bib25","doi-asserted-by":"crossref","DOI":"10.1155\/2017\/4209257","article-title":"Association of GCLM -588C\/T and GCLC -129T\/C promoter polymorphisms of genes coding the subunits of glutamate cysteine Ligase with ischemic heart disease development in Kazakhstan population","volume":"2017","author":"Skvortsova","year":"2017","journal-title":"Dis. Markers"},{"key":"10.1016\/j.bbrc.2025.151900_bib26","first-page":"100","article-title":"[The relationship between the polymorphism of glutamate cysteine ligase modulatory subunit gene and the susceptibility to chronic obstructive pulmonary disease]","volume":"29","author":"Hu","year":"2006","journal-title":"Zhonghua Jiehe He Huxi Zazhi"},{"key":"10.1016\/j.bbrc.2025.151900_bib27","doi-asserted-by":"crossref","first-page":"5793","DOI":"10.1007\/s11033-020-05647-5","article-title":"Genetic variants in glutamate cysteine ligase confer protection against type 2 diabetes","volume":"47","author":"Azarova","year":"2020","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.bbrc.2025.151900_bib28","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1097\/YPG.0b013e32832cef21","article-title":"Mutation screening of the glutamate cysteine ligase modifier (GCLM) gene in patients with schizophrenia","volume":"19","author":"Butticaz","year":"2009","journal-title":"Psychiatr. Genet."},{"key":"10.1016\/j.bbrc.2025.151900_bib29","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1093\/cvr\/cvs333","article-title":"Chronic depletion of glutathione exacerbates ventricular remodelling and dysfunction in the pressure-overloaded heart","volume":"97","author":"Watanabe","year":"2013","journal-title":"Cardiovasc. Res."}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X2500614X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X2500614X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T00:15:12Z","timestamp":1746490512000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X2500614X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":29,"alternative-id":["S0006291X2500614X"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2025.151900","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Gene-dose effect of the glutathione biosynthesis gene on ascorbate deficiency in mice","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2025.151900","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"151900"}}