{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T03:52:39Z","timestamp":1773114759274,"version":"3.50.1"},"reference-count":109,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T00:00:00Z","timestamp":1755043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010559","name":"Children's Hospital of Pittsburgh","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010559","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100003287","name":"Rally Foundation","doi-asserted-by":"publisher","award":["22IN42"],"award-info":[{"award-number":["22IN42"]}],"id":[{"id":"10.13039\/100003287","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007408","name":"Medical Center, University of Pittsburgh","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007408","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007921","name":"University of Pittsburgh","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007921","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.bbrc.2025.152478","type":"journal-article","created":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T06:39:35Z","timestamp":1755067175000},"page":"152478","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Efficient derivation of immortalized, isogenic cell lines from genetically defined murine hepatoblastomas"],"prefix":"10.1016","volume":"780","author":[{"given":"Huabo","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8300-8288","authenticated-orcid":false,"given":"Keyao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingwei","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colin","family":"Henchy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Knapp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6214-9167","authenticated-orcid":false,"given":"Sarangarajan","family":"Ranganathan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9666-8205","authenticated-orcid":false,"given":"Edward V.","family":"Prochownik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2025.152478_bib1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.3727\/105221616X693639","article-title":"Novel advances in understanding of molecular pathogenesis of hepatoblastoma: a Wnt\/beta-Catenin perspective","volume":"17","author":"Bell","year":"2017","journal-title":"Gene Expr."},{"key":"10.1016\/j.bbrc.2025.152478_bib2","doi-asserted-by":"crossref","first-page":"445","DOI":"10.5582\/bst.2023.01311","article-title":"An update on diagnosis and treatment of hepatoblastoma","volume":"17","author":"Cao","year":"2024","journal-title":"Biosci. Trends"},{"key":"10.1016\/j.bbrc.2025.152478_bib3","doi-asserted-by":"crossref","first-page":"23","DOI":"10.17998\/jlc.2022.02.24","article-title":"Recent updates on the classification of hepatoblastoma according to the international pediatric liver tumors consensus","volume":"22","author":"Jeong","year":"2022","journal-title":"J Liver Cancer"},{"key":"10.1016\/j.bbrc.2025.152478_bib4","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1038\/modpathol.2013.80","article-title":"Towards an international pediatric liver tumor consensus classification: proceedings of the Los Angeles COG liver tumors symposium","volume":"27","author":"Lopez-Terrada","year":"2014","journal-title":"Mod. Pathol."},{"key":"10.1016\/j.bbrc.2025.152478_bib5","doi-asserted-by":"crossref","DOI":"10.3390\/cancers13091996","article-title":"Reconciling the biological and transcriptional variability of hepatoblastoma with its mutational uniformity","volume":"13","author":"Prochownik","year":"2021","journal-title":"Cancers (Basel)"},{"key":"10.1016\/j.bbrc.2025.152478_bib6","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1177\/1093526619875228","article-title":"Hepatoblastoma and pediatric hepatocellular carcinoma: an update","volume":"23","author":"Ranganathan","year":"2020","journal-title":"Pediatr. Dev. Pathol."},{"key":"10.1016\/j.bbrc.2025.152478_bib7","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.ccr.2008.11.002","article-title":"Hepatic stem-like phenotype and interplay of Wnt\/beta-catenin and Myc signaling in aggressive childhood liver cancer","volume":"14","author":"Cairo","year":"2008","journal-title":"Cancer Cell"},{"key":"10.1016\/j.bbrc.2025.152478_bib8","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.jhep.2020.03.025","article-title":"Epigenetic footprint enables molecular risk stratification of hepatoblastoma with clinical implications","volume":"73","author":"Carrillo-Reixach","year":"2020","journal-title":"J. Hepatol."},{"key":"10.1016\/j.bbrc.2025.152478_bib9","doi-asserted-by":"crossref","DOI":"10.1002\/pbc.30505","article-title":"Consensus classification of pediatric hepatocellular tumors: a report from the Children's hepatic tumors International collaboration (CHIC)","author":"Cho","year":"2023","journal-title":"Pediatr. Blood Cancer"},{"key":"10.1016\/j.bbrc.2025.152478_bib10","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1002\/hep.29672","article-title":"New insights into diagnosis and therapeutic options for proliferative hepatoblastoma","volume":"68","author":"Hooks","year":"2018","journal-title":"Hepatology"},{"key":"10.1016\/j.bbrc.2025.152478_bib11","doi-asserted-by":"crossref","DOI":"10.3390\/cancers12030720","article-title":"Liver transplantation for pediatric liver cancer","volume":"12","author":"Sindhi","year":"2020","journal-title":"Cancers (Basel)"},{"key":"10.1016\/j.bbrc.2025.152478_bib12","doi-asserted-by":"crossref","first-page":"pkz007","DOI":"10.1093\/jncics\/pkz007","article-title":"Trends in international incidence of pediatric cancers in children under 5 years of age: 1988-2012","volume":"3","author":"Hubbard","year":"2019","journal-title":"JNCI Cancer Spectr."},{"key":"10.1016\/j.bbrc.2025.152478_bib13","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1007\/s11912-022-01230-2","article-title":"Current approaches in hepatoblastoma-new biological insights to inform therapy","volume":"24","author":"Wu","year":"2022","journal-title":"Curr. Oncol. Rep."},{"key":"10.1016\/j.bbrc.2025.152478_bib14","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1016\/S0002-9440(10)63008-8","article-title":"Distinctive molecular genetic alterations in sporadic and familial adenomatous polyposis-associated pancreatoblastomas : frequent alterations in the APC\/beta-catenin pathway and chromosome 11p","volume":"159","author":"Abraham","year":"2001","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.bbrc.2025.152478_bib15","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1002\/mpo.10180","article-title":"Genetic alterations in hepatoblastoma and hepatocellular carcinoma: common and distinctive aspects","volume":"39","author":"Buendia","year":"2002","journal-title":"Med. Pediatr. Oncol."},{"key":"10.1016\/j.bbrc.2025.152478_bib16","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1016\/j.jhep.2014.08.009","article-title":"The genomic landscape of hepatoblastoma and their progenies with HCC-like features","volume":"61","author":"Eichenmuller","year":"2014","journal-title":"J. Hepatol."},{"key":"10.1016\/j.bbrc.2025.152478_bib17","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/S1386-6346(02)00264-4","article-title":"Sequence variants of the Axin gene in hepatoblastoma","volume":"25","author":"Miao","year":"2003","journal-title":"Hepatol. Res."},{"key":"10.1016\/j.bbrc.2025.152478_bib18","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1111\/j.1478-3231.2011.02646.x","article-title":"Deregulation of hippo kinase signalling in human hepatic malignancies","volume":"32","author":"Li","year":"2012","journal-title":"Liver Int."},{"key":"10.1016\/j.bbrc.2025.152478_bib19","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1053\/j.gastro.2014.05.004","article-title":"Activation of beta-catenin and Yap1 in human hepatoblastoma and induction of hepatocarcinogenesis in mice","volume":"147","author":"Tao","year":"2014","journal-title":"Gastroenterology"},{"key":"10.1016\/j.bbrc.2025.152478_bib20","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.jcmgh.2021.02.004","article-title":"Patient-derived mutant forms of NFE2L2\/NRF2 drive aggressive murine hepatoblastomas","volume":"12","author":"Wang","year":"2021","journal-title":"Cell. Mol. Gastroenterol. Hepatol."},{"key":"10.1016\/j.bbrc.2025.152478_bib21","doi-asserted-by":"crossref","first-page":"17524","DOI":"10.1074\/jbc.RA119.009979","article-title":"beta-Catenin mutations as determinants of hepatoblastoma phenotypes in mice","volume":"294","author":"Zhang","year":"2019","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2025.152478_bib22","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1038\/nature25480","article-title":"The landscape of genomic alterations across childhood cancers","volume":"555","author":"Grobner","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.bbrc.2025.152478_bib23","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1038\/nature25795","article-title":"Pan-cancer genome and transcriptome analyses of 1,699 paediatric leukaemias and solid tumours","volume":"555","author":"Ma","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.bbrc.2025.152478_bib24","doi-asserted-by":"crossref","first-page":"534","DOI":"10.4081\/oncol.2021.534","article-title":"Adenomatous polyposis coli in cancer and therapeutic implications","volume":"15","author":"Noe","year":"2021","journal-title":"Oncol. Rev."},{"key":"10.1016\/j.bbrc.2025.152478_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2021.100782","article-title":"Regulation of Wnt receptor activity: implications for therapeutic development in colon cancer","volume":"296","author":"Zhong","year":"2021","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2025.152478_bib26","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.5858\/arpa.2018-0570-RA","article-title":"Familial adenomatous polyposis syndrome: an update and review of extraintestinal manifestations","volume":"143","author":"Dinarvand","year":"2019","journal-title":"Arch. Pathol. Lab Med."},{"key":"10.1016\/j.bbrc.2025.152478_bib27","doi-asserted-by":"crossref","DOI":"10.1002\/pbc.26892","article-title":"Germline APC mutations in hepatoblastoma","volume":"65","author":"Yang","year":"2018","journal-title":"Pediatr. Blood Cancer"},{"key":"10.1016\/j.bbrc.2025.152478_bib28","doi-asserted-by":"crossref","first-page":"22","DOI":"10.3389\/fped.2016.00022","article-title":"Hepatoblastoma: a need for cell lines and tissue banks to develop targeted drug therapies","volume":"4","author":"Rikhi","year":"2016","journal-title":"Front. Pediatr."},{"key":"10.1016\/j.bbrc.2025.152478_bib29","doi-asserted-by":"crossref","first-page":"4003","DOI":"10.1038\/s41467-023-39717-6","article-title":"Genome-wide mapping of cancer dependency genes and genetic modifiers of chemotherapy in high-risk hepatoblastoma","volume":"14","author":"Fang","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bbrc.2025.152478_bib30","doi-asserted-by":"crossref","DOI":"10.3390\/ijms222313135","article-title":"The curious case of the HepG2 cell line: 40 years of expertise","volume":"22","author":"Arzumanian","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.bbrc.2025.152478_bib31","first-page":"1","article-title":"Establishment of a cell line and its clonal sublines from a patient with hepatoblastoma","volume":"67","author":"Doi","year":"1976","journal-title":"Gan"},{"key":"10.1016\/j.bbrc.2025.152478_bib32","first-page":"809","article-title":"Characterization of the continuous cell line HepT1 derived from a human hepatoblastoma","volume":"74","author":"Pietsch","year":"1996","journal-title":"Lab. Invest."},{"key":"10.1016\/j.bbrc.2025.152478_bib33","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1038\/282615a0","article-title":"Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line","volume":"282","author":"Aden","year":"1979","journal-title":"Nature"},{"key":"10.1016\/j.bbrc.2025.152478_bib34","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1016\/j.humpath.2009.07.003","article-title":"Hep G2 is a hepatoblastoma-derived cell line","volume":"40","author":"Lopez-Terrada","year":"2009","journal-title":"Hum. Pathol."},{"key":"10.1016\/j.bbrc.2025.152478_bib35","doi-asserted-by":"crossref","first-page":"10068","DOI":"10.1074\/jbc.M117.782052","article-title":"Sequential adaptive changes in a c-Myc-driven model of hepatocellular carcinoma","volume":"292","author":"Dolezal","year":"2017","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.bbrc.2025.152478_bib36","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1046\/j.1474-9728.2003.00048.x","article-title":"Involvement of the INK4a\/Arf gene locus in senescence","volume":"2","author":"Collins","year":"2003","journal-title":"Aging Cell"},{"key":"10.1016\/j.bbrc.2025.152478_bib37","doi-asserted-by":"crossref","DOI":"10.3390\/biom9030087","article-title":"Dual role of the alternative reading frame ARF protein in cancer","volume":"9","author":"Fontana","year":"2019","journal-title":"Biomolecules"},{"key":"10.1016\/j.bbrc.2025.152478_bib38","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1038\/35004020","article-title":"p16INK4A and p19ARF act in overlapping pathways in cellular immortalization","volume":"2","author":"Carnero","year":"2000","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.bbrc.2025.152478_bib39","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1101\/sqb.2005.70.004","article-title":"p53-Dependent and -independent functions of the Arf tumor suppressor","volume":"70","author":"Sherr","year":"2005","journal-title":"Cold Spring Harbor Symp. Quant. Biol."},{"key":"10.1016\/j.bbrc.2025.152478_bib40","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S1535-6108(02)00102-2","article-title":"The RB and p53 pathways in cancer","volume":"2","author":"Sherr","year":"2002","journal-title":"Cancer Cell"},{"key":"10.1016\/j.bbrc.2025.152478_bib41","doi-asserted-by":"crossref","DOI":"10.3390\/genes11070824","article-title":"P14(ARF): the absence that makes the difference","volume":"11","author":"Cilluffo","year":"2020","journal-title":"Genes"},{"key":"10.1016\/j.bbrc.2025.152478_bib42","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1002\/hep.510270414","article-title":"Analysis of CDKN2A, CDKN2B, CDKN2C, and cyclin Ds gene status in hepatoblastoma","volume":"27","author":"Iolascon","year":"1998","journal-title":"Hepatology"},{"key":"10.1016\/j.bbrc.2025.152478_bib43","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0182705","article-title":"Ribosomopathy-like properties of murine and human cancers","volume":"12","author":"Kulkarni","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.bbrc.2025.152478_bib44","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1097\/01.MP.0000066799.99032.A7","article-title":"Hypermethylation of the p16 gene and lack of p16 expression in hepatoblastoma","volume":"16","author":"Shim","year":"2003","journal-title":"Mod. Pathol."},{"key":"10.1016\/j.bbrc.2025.152478_bib45","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/j.cell.2019.10.005","article-title":"Cellular senescence: defining a path forward","volume":"179","author":"Gorgoulis","year":"2019","journal-title":"Cell"},{"key":"10.1016\/j.bbrc.2025.152478_bib46","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/S0955-0674(00)00155-1","article-title":"Genes involved in senescence and immortalization","volume":"12","author":"Lundberg","year":"2000","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.bbrc.2025.152478_bib47","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1002\/hep.20084","article-title":"Immortalized p19ARF null hepatocytes restore liver injury and generate hepatic progenitors after transplantation","volume":"39","author":"Mikula","year":"2004","journal-title":"Hepatology"},{"key":"10.1016\/j.bbrc.2025.152478_bib48","doi-asserted-by":"crossref","first-page":"160","DOI":"10.18632\/aging.100129","article-title":"How to become immortal: let MEFs count the ways","volume":"2","author":"Odell","year":"2010","journal-title":"Aging (Albany NY)"},{"key":"10.1016\/j.bbrc.2025.152478_bib49","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1038\/nrc3960","article-title":"Forging a signature of in vivo senescence","volume":"15","author":"Sharpless","year":"2015","journal-title":"Nat. Rev. Cancer"},{"key":"10.1016\/j.bbrc.2025.152478_bib50","doi-asserted-by":"crossref","first-page":"2424","DOI":"10.1101\/gad.12.15.2424","article-title":"Myc signaling via the ARF tumor suppressor regulates p53-dependent apoptosis and immortalization","volume":"12","author":"Zindy","year":"1998","journal-title":"Genes Dev."},{"key":"10.1016\/j.bbrc.2025.152478_bib51","doi-asserted-by":"crossref","first-page":"2497","DOI":"10.4161\/cc.10.15.16776","article-title":"The meaning of p16(ink4a) expression in tumors: functional significance, clinical associations and future developments","volume":"10","author":"Witkiewicz","year":"2011","journal-title":"Cell Cycle"},{"key":"10.1016\/j.bbrc.2025.152478_bib52","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0077675","article-title":"Rapid in vitro derivation of endothelium directly from human cancer cells","volume":"8","author":"Elster","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.bbrc.2025.152478_bib53","doi-asserted-by":"crossref","first-page":"W242","DOI":"10.1093\/nar\/gky354","article-title":"CRISPOR: intuitive guide selection for CRISPR\/Cas9 genome editing experiments and screens","volume":"46","author":"Concordet","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.bbrc.2025.152478_bib54","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1038\/s41587-020-0439-x","article-title":"The nf-core framework for community-curated bioinformatics pipelines","volume":"38","author":"Ewels","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.bbrc.2025.152478_bib55","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1038\/s41587-020-0546-8","article-title":"Visualizing and interpreting cancer genomics data via the Xena platform","volume":"38","author":"Goldman","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.bbrc.2025.152478_bib56","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1101\/gad.6.2.177","article-title":"The interaction of RB with E2F coincides with an inhibition of the transcriptional activity of E2F","volume":"6","author":"Hiebert","year":"1992","journal-title":"Genes Dev."},{"key":"10.1016\/j.bbrc.2025.152478_bib57","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1128\/MCB.02147-06","article-title":"E2f1, E2f2, and E2f3 control E2F target expression and cellular proliferation via a p53-dependent negative feedback loop","volume":"27","author":"Timmers","year":"2007","journal-title":"Mol. Cell Biol."},{"key":"10.1016\/j.bbrc.2025.152478_bib58","doi-asserted-by":"crossref","first-page":"1974","DOI":"10.1101\/gad.11.15.1974","article-title":"Repression of p53-mediated transcription by MDM2: a dual mechanism","volume":"11","author":"Thut","year":"1997","journal-title":"Genes Dev."},{"key":"10.1016\/j.bbrc.2025.152478_bib59","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1038\/sj.onc.1206927","article-title":"The tumor suppressor p16(INK4a) gene is a regulator of apoptosis induced by ultraviolet light and cisplatin","volume":"23","author":"Al-Mohanna","year":"2004","journal-title":"Oncogene"},{"key":"10.1016\/j.bbrc.2025.152478_bib60","doi-asserted-by":"crossref","first-page":"5947","DOI":"10.1073\/pnas.0601273103","article-title":"Both p16(Ink4a) and the p19(Arf)-p53 pathway constrain progression of pancreatic adenocarcinoma in the mouse","volume":"103","author":"Bardeesy","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.bbrc.2025.152478_bib61","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/S0014-5793(02)03270-2","article-title":"E2F1 pathways to apoptosis","volume":"529","author":"Ginsberg","year":"2002","journal-title":"FEBS Lett."},{"key":"10.1016\/j.bbrc.2025.152478_bib62","doi-asserted-by":"crossref","DOI":"10.3390\/biom10030420","article-title":"Role of p53 in the regulation of cellular senescence","volume":"10","author":"Mijit","year":"2020","journal-title":"Biomolecules"},{"key":"10.1016\/j.bbrc.2025.152478_bib63","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1016\/S0092-8674(00)80452-3","article-title":"Tumor suppression at the mouse INK4a locus mediated by the alternative reading frame product p19ARF","volume":"91","author":"Kamijo","year":"1997","journal-title":"Cell"},{"key":"10.1016\/j.bbrc.2025.152478_bib64","doi-asserted-by":"crossref","first-page":"10184","DOI":"10.1073\/pnas.1004796107","article-title":"Tissue-specific p19Arf regulation dictates the response to oncogenic K-ras","volume":"107","author":"Young","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.bbrc.2025.152478_bib65","doi-asserted-by":"crossref","DOI":"10.3390\/cells12071001","article-title":"Cancer spheroids and organoids as novel tools for research and therapy: state of the art and challenges to guide precision medicine","volume":"12","author":"El Harane","year":"2023","journal-title":"Cells"},{"key":"10.1016\/j.bbrc.2025.152478_bib66","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1175503","article-title":"3D cancer models: one step closer to in vitro human studies","volume":"14","author":"Manduca","year":"2023","journal-title":"Front. Immunol."},{"key":"10.1016\/j.bbrc.2025.152478_bib67","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1016\/j.ccell.2021.07.020","article-title":"Tumor organoids: opportunities and challenges to guide precision medicine","volume":"39","author":"Veninga","year":"2021","journal-title":"Cancer Cell"},{"key":"10.1016\/j.bbrc.2025.152478_bib68","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.7150\/thno.91362","article-title":"Construction of tumor organoids and their application to cancer research and therapy","volume":"14","author":"Lv","year":"2024","journal-title":"Theranostics"},{"key":"10.1016\/j.bbrc.2025.152478_bib69","doi-asserted-by":"crossref","DOI":"10.3390\/cancers12092668","article-title":"A human organoid model of aggressive hepatoblastoma for disease modeling and drug testing","volume":"12","author":"Saltsman","year":"2020","journal-title":"Cancers (Basel)"},{"key":"10.1016\/j.bbrc.2025.152478_bib70","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1111\/cas.13155","article-title":"Tumor-derived spheroids: relevance to cancer stem cells and clinical applications","volume":"108","author":"Ishiguro","year":"2017","journal-title":"Cancer Sci."},{"key":"10.1016\/j.bbrc.2025.152478_bib71","doi-asserted-by":"crossref","first-page":"2268","DOI":"10.1002\/ijc.25251","article-title":"Endothelial-like cells derived directly from human tumor xenografts","volume":"127","author":"Sajithlal","year":"2010","journal-title":"Int. J. Cancer"},{"key":"10.1016\/j.bbrc.2025.152478_bib72","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/978-3-030-44518-8_7","article-title":"Endothelial progenitors in the tumor microenvironment","volume":"1263","author":"Testa","year":"2020","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.bbrc.2025.152478_bib73","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1053\/j.gastro.2014.07.026","article-title":"Hippo\/YAP, beta-catenin, and the cancer cell: a \"menage a trois\" in hepatoblastoma","volume":"147","author":"Sylvester","year":"2014","journal-title":"Gastroenterology"},{"key":"10.1016\/j.bbrc.2025.152478_bib74","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1002\/humu.10149","article-title":"Genetics, eMelanoBase: an online locus-specific variant database for familial melanoma","volume":"21","author":"Fung","year":"2003","journal-title":"Hum. Mutat."},{"key":"10.1016\/j.bbrc.2025.152478_bib75","doi-asserted-by":"crossref","first-page":"37","DOI":"10.4103\/tme.tme_24_17","article-title":"Aberrant expression of p14(ARF) in human cancers: a new biomarker?","volume":"1","author":"Inoue","year":"2018","journal-title":"Tumor Microenviron."},{"key":"10.1016\/j.bbrc.2025.152478_bib76","doi-asserted-by":"crossref","DOI":"10.15761\/CRR.1000145","article-title":"Aberrant expression of p16(INK4a) in human cancers - a new biomarker?","volume":"2","author":"Inoue","year":"2018","journal-title":"Cancer Rep. Rev."},{"key":"10.1016\/j.bbrc.2025.152478_bib77","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.bbrc.2009.11.020","article-title":"e2f1 Gene is a new member of Wnt\/beta-catenin\/Tcf-regulated genes","volume":"391","author":"Abramova","year":"2010","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2025.152478_bib78","doi-asserted-by":"crossref","first-page":"2700","DOI":"10.1038\/s41467-024-46531-1","article-title":"Expected and unexpected effects after systemic inhibition of Hippo transcriptional output in cancer","volume":"15","author":"Baroja","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bbrc.2025.152478_bib79","doi-asserted-by":"crossref","first-page":"3054","DOI":"10.1093\/emboj\/18.11.3054","article-title":"Excess beta-catenin promotes accumulation of transcriptionally active p53","volume":"18","author":"Damalas","year":"1999","journal-title":"EMBO J."},{"key":"10.1016\/j.bbrc.2025.152478_bib80","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1158\/1541-7786.MCR-15-0305","article-title":"Control of proliferation and cancer growth by the hippo signaling pathway","volume":"14","author":"Ehmer","year":"2016","journal-title":"Mol. Cancer Res."},{"key":"10.1016\/j.bbrc.2025.152478_bib81","doi-asserted-by":"crossref","DOI":"10.3390\/genes10030244","article-title":"MYC oncogene contributions to release of cell cycle brakes","volume":"10","author":"Garcia-Gutierrez","year":"2019","journal-title":"Genes"},{"key":"10.1016\/j.bbrc.2025.152478_bib82","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23031905","article-title":"The expression of TP53-Induced glycolysis and apoptosis regulator (TIGAR) can be controlled by the antioxidant orchestrator NRF2 in human carcinoma cells","volume":"23","author":"Simon-Molas","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.bbrc.2025.152478_bib83","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1016\/S1097-2765(02)00520-8","article-title":"c-Myc can induce DNA damage, increase reactive oxygen species, and mitigate p53 function: a mechanism for oncogene-induced genetic instability","volume":"9","author":"Vafa","year":"2002","journal-title":"Mol. Cell"},{"key":"10.1016\/j.bbrc.2025.152478_bib84","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1053\/jhep.2003.50138","article-title":"Homozygous deletion scanning in hepatobiliary tumor cell lines reveals alternative pathways for liver carcinogenesis","volume":"37","author":"Pineau","year":"2003","journal-title":"Hepatology"},{"key":"10.1016\/j.bbrc.2025.152478_bib85","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1038\/s41392-023-01309-7","article-title":"CRISPR\/Cas9 therapeutics: progress and prospects","volume":"8","author":"Li","year":"2023","journal-title":"Signal Transduct. Targeted Ther."},{"key":"10.1016\/j.bbrc.2025.152478_bib86","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1038\/s42003-019-0705-y","article-title":"Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair","volume":"2","author":"Sansbury","year":"2019","journal-title":"Commun. Biol."},{"key":"10.1016\/j.bbrc.2025.152478_bib87","doi-asserted-by":"crossref","first-page":"2936","DOI":"10.1093\/emboj\/cdf289","article-title":"INK4a-deficient human diploid fibroblasts are resistant to RAS-induced senescence","volume":"21","author":"Brookes","year":"2002","journal-title":"EMBO J."},{"key":"10.1016\/j.bbrc.2025.152478_bib88","doi-asserted-by":"crossref","first-page":"9608","DOI":"10.1158\/0008-5472.CAN-08-2263","article-title":"The ARF tumor suppressor can promote the progression of some tumors","volume":"68","author":"Humbey","year":"2008","journal-title":"Cancer Res."},{"key":"10.1016\/j.bbrc.2025.152478_bib89","first-page":"865","article-title":"[Regulation of p16INK4a, senescence and oncogenesis]","volume":"22","author":"Chien","year":"2006","journal-title":"Med. Sci."},{"key":"10.1016\/j.bbrc.2025.152478_bib90","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1038\/nrm1987","article-title":"Regulation of the INK4b-ARF-INK4a tumour suppressor locus: all for one or one for all","volume":"7","author":"Gil","year":"2006","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.bbrc.2025.152478_bib91","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1134\/S0006297918110019","article-title":"Regulation of tumor suppressor gene CDKN2A and encoded p16-INK4a protein by covalent modifications","volume":"83","author":"Jiao","year":"2018","journal-title":"Biochemistry (Mosc)"},{"key":"10.1016\/j.bbrc.2025.152478_bib92","doi-asserted-by":"crossref","first-page":"2277","DOI":"10.3892\/ol.2015.3617","article-title":"Effect of phosphorylation and methylation on the function of the p16(INK4a) protein in non-small cell lung cancer A549 cells","volume":"10","author":"Ma","year":"2015","journal-title":"Oncol. Lett."},{"key":"10.1016\/j.bbrc.2025.152478_bib93","doi-asserted-by":"crossref","DOI":"10.3390\/biom10081143","article-title":"Post-translational regulation of ARF: perspective in cancer","volume":"10","author":"Seo","year":"2020","journal-title":"Biomolecules"},{"key":"10.1016\/j.bbrc.2025.152478_bib94","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1038\/s41392-023-01332-8","article-title":"Hypoxic microenvironment in cancer: molecular mechanisms and therapeutic interventions","volume":"8","author":"Chen","year":"2023","journal-title":"Signal Transduct. Targeted Ther."},{"key":"10.1016\/j.bbrc.2025.152478_bib95","doi-asserted-by":"crossref","DOI":"10.1016\/j.drup.2021.100787","article-title":"Hypoxia as a driver of resistance to immunotherapy","volume":"59","author":"Kopecka","year":"2021","journal-title":"Drug Resist. Updates"},{"key":"10.1016\/j.bbrc.2025.152478_bib96","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1016\/j.tcb.2017.06.003","article-title":"Metabolic interactions in the tumor microenvironment","volume":"27","author":"Lyssiotis","year":"2017","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.bbrc.2025.152478_bib97","doi-asserted-by":"crossref","first-page":"562","DOI":"10.3389\/fonc.2020.00562","article-title":"Tumor hypoxia: impact on radiation therapy and molecular pathways","volume":"10","author":"Sorensen","year":"2020","journal-title":"Front. Oncol."},{"key":"10.1016\/j.bbrc.2025.152478_bib98","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1038\/s41580-023-00631-w","article-title":"Biology and therapeutic targeting of vascular endothelial growth factor A","volume":"24","author":"Perez-Gutierrez","year":"2023","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.bbrc.2025.152478_bib99","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1186\/s12885-023-10659-y","article-title":"Characteristics of vasculogenic mimicry and tumour to endothelial transdifferentiation in human glioblastoma: a systematic review","volume":"23","author":"Maddison","year":"2023","journal-title":"BMC Cancer"},{"key":"10.1016\/j.bbrc.2025.152478_bib100","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s12943-020-01288-1","article-title":"Mechanisms of vasculogenic mimicry in hypoxic tumor microenvironments","volume":"20","author":"Wei","year":"2021","journal-title":"Mol. Cancer"},{"key":"10.1016\/j.bbrc.2025.152478_bib101","first-page":"45","article-title":"Correlation between glioblastoma stem-like cells and tumor vascularization","volume":"27","author":"He","year":"2012","journal-title":"Oncol. Rep."},{"key":"10.1016\/j.bbrc.2025.152478_bib102","doi-asserted-by":"crossref","first-page":"5423","DOI":"10.1038\/s41467-021-25430-9","article-title":"Genetic and epigenetic basis of hepatoblastoma diversity","volume":"12","author":"Nagae","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.bbrc.2025.152478_bib103","doi-asserted-by":"crossref","first-page":"2376","DOI":"10.1056\/NEJMoa1801109","article-title":"Sodium thiosulfate for protection from cisplatin-induced hearing loss","volume":"378","author":"Brock","year":"2018","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.bbrc.2025.152478_bib104","doi-asserted-by":"crossref","first-page":"470","DOI":"10.2741\/e392","article-title":"Hepatoblastoma throughout SIOPEL trials - clinical lessons learnt","volume":"4","author":"Czauderna","year":"2012","journal-title":"Front Biosci (Elite Ed)"},{"key":"10.1016\/j.bbrc.2025.152478_bib105","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/S1470-2045(18)30895-7","article-title":"Minimal adjuvant chemotherapy for children with hepatoblastoma resected at diagnosis (AHEP0731): a Children's oncology group, multicentre, phase 3 trial","volume":"20","author":"Katzenstein","year":"2019","journal-title":"Lancet Oncol."},{"key":"10.1016\/j.bbrc.2025.152478_bib106","doi-asserted-by":"crossref","first-page":"1662","DOI":"10.1056\/NEJMoa0810613","article-title":"Cisplatin versus cisplatin plus doxorubicin for standard-risk hepatoblastoma","volume":"361","author":"Perilongo","year":"2009","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.bbrc.2025.152478_bib107","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1016\/S1470-2045(13)70272-9","article-title":"Childhood liver tumours strategy, dose-dense cisplatin-based chemotherapy and surgery for children with high-risk hepatoblastoma (SIOPEL-4): a prospective, single-arm, feasibility study","volume":"14","author":"Zsiros","year":"2013","journal-title":"Lancet Oncol."},{"key":"10.1016\/j.bbrc.2025.152478_bib108","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1002\/hep.25776","article-title":"Oncogene-specific formation of chemoresistant murine hepatic cancer stem cells","volume":"56","author":"Chow","year":"2012","journal-title":"Hepatology"},{"key":"10.1016\/j.bbrc.2025.152478_bib109","doi-asserted-by":"crossref","first-page":"2524","DOI":"10.1158\/2159-8290.CD-20-1809","article-title":"Integrated genomic analysis identifies driver genes and cisplatin-resistant progenitor phenotype in pediatric liver cancer","volume":"11","author":"Hirsch","year":"2021","journal-title":"Cancer Discov."}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X25011933?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X25011933?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T12:29:48Z","timestamp":1772800188000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X25011933"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":109,"alternative-id":["S0006291X25011933"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2025.152478","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.09.29.615692","asserted-by":"object"}]},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Efficient derivation of immortalized, isogenic cell lines from genetically defined murine hepatoblastomas","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2025.152478","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"152478"}}