{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T13:12:46Z","timestamp":1778159566385,"version":"3.51.4"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100020647","name":"Autonomous University of Juarez City","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020647","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.bbrc.2025.152583","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T16:00:54Z","timestamp":1756828854000},"page":"152583","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["The antipsychotic chlorpromazine induces oxidative stress in endothelial cells: an in vitro assessment employing Fourier-transform infrared spectroscopy"],"prefix":"10.1016","volume":"782","author":[{"given":"Guillermo","family":"Barraza-Garza","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro","family":"Olivas-Corral","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3643-2668","authenticated-orcid":false,"given":"David L.","family":"Mel\u00e9ndez-Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3256-3257","authenticated-orcid":false,"given":"Jorge A.","family":"Sierra-Fonseca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1619-5004","authenticated-orcid":false,"given":"Luis F.","family":"Plenge-Tellechea","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2025.152583_bib1","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/s00249-015-1029-6","article-title":"EPR spectroscopy of chlorpromazine-induced free radical formation in normal human melanocytes","volume":"44","author":"Otr\u0119ba","year":"2015","journal-title":"Eur. Biophys. J."},{"key":"10.1016\/j.bbrc.2025.152583_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/brainsci10100742","article-title":"Regulation of reactive oxygen species\u2010mediated damage in the pathogenesis of schizophrenia","volume":"10","author":"Madireddy","year":"2020","journal-title":"Brain Sci."},{"key":"10.1016\/j.bbrc.2025.152583_bib3","first-page":"97","article-title":"CREM, PRM I and II gene expression in Wistar rats testes treated with antipsychotic drugs: chlorpromazine, Rauwolfia vomitoria and co-administration of reserpine, zinc and ascorbic acid","volume":"25","author":"Opeyemi","year":"2021","journal-title":"JBRA Assist. Reprod."},{"key":"10.1016\/j.bbrc.2025.152583_bib4","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1038\/nrn.2016.57","article-title":"Dysregulation of the dopamine system in the pathophysiology of schizophrenia and depression","volume":"17","author":"Grace","year":"2016","journal-title":"Nat. Rev. Neurosci."},{"key":"10.1016\/j.bbrc.2025.152583_bib5","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1176\/appi.ajp.20240738","article-title":"Antipsychotic drugs: a concise review of history, classification, indications, mechanism, efficacy, side effects, dosing, and clinical application","volume":"181","author":"Leucht","year":"2024","journal-title":"Am. J. Psychiatr."},{"key":"10.1016\/j.bbrc.2025.152583_bib6","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/0192-0561(90)90052-O","article-title":"A comparison of the effects of chlorpromazine and more selective histamine and 5-hydroxytryptamine antagonists on human IgG synthesis in vitro","volume":"12","author":"Martinez","year":"1990","journal-title":"Int. J. Immunopharm."},{"key":"10.1016\/j.bbrc.2025.152583_bib7","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1124\/mol.52.3.482","article-title":"Methylene blue is a muscarinic antagonist in cardiac myocytes","volume":"52","author":"Abi-Gerges","year":"1997","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.bbrc.2025.152583_bib8","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1111\/j.1751-1097.2007.00077.x","article-title":"Chlorpromazine binding to Na+, K+-ATPase and photolabeling: involvement of the ouabain site monitored by fluorescence","volume":"83","author":"Guevara","year":"2007","journal-title":"Photochem. Photobiol."},{"key":"10.1016\/j.bbrc.2025.152583_bib9","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/s10863-017-9741-9","article-title":"Chlorpromazine and dimethyl sulfoxide modulate the catalytic activity of the plasma membrane Ca2+-ATPase from human erythrocyte","volume":"50","author":"Plenge-Tellechea","year":"2018","journal-title":"J. Bioenerg. Biomembr."},{"key":"10.1016\/j.bbrc.2025.152583_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbrc.2025.151344","article-title":"Direct effects of antipsychotics on potassium channels","volume":"749","author":"Zhuang","year":"2025","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.bbrc.2025.152583_bib11","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1021\/acschemneuro.8b00258","article-title":"Classics in chemical neuroscience: chlorpromazine","volume":"10","author":"Boyd-Kimball","year":"2019","journal-title":"ACS Chem. Neurosci."},{"key":"10.1016\/j.bbrc.2025.152583_bib12","first-page":"36","article-title":"The potential risks of commonly prescribed antipsychotics: during pregnancy and lactation","volume":"2","author":"Iqbal","year":"2005","journal-title":"Psychiatry (Edgmont)."},{"key":"10.1016\/j.bbrc.2025.152583_bib13","doi-asserted-by":"crossref","first-page":"1518","DOI":"10.1002\/hep.26160","article-title":"Oxidative stress plays a major role in chlorpromazine-induced cholestasis in human HepaRG cells","volume":"57","author":"Anth\u00e9rieu","year":"2013","journal-title":"Hepatology"},{"key":"10.1016\/j.bbrc.2025.152583_bib14","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1538\/expanim.16-0010","article-title":"Effects of agmatine on chlorpromazine toxicity in the liver of wistar rats: the possible role of oxidant\/antioxidant imbalance","volume":"66","author":"Bratislav","year":"2017","journal-title":"Exp. Anim."},{"key":"10.1016\/j.bbrc.2025.152583_bib15","doi-asserted-by":"crossref","DOI":"10.1155\/2022\/6886752","article-title":"Chlorpromazine and promethazine (C+P) reduce brain injury after ischemic stroke through the PKC- \u03b4\/NOX\/MnSOD pathway","author":"Guo","year":"2022","journal-title":"Mediat. Inflamm."},{"key":"10.1016\/j.bbrc.2025.152583_bib16","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.tiv.2017.07.008","article-title":"The role of oxidative stress in antipsychotics induced ovarian toxicity","volume":"44","author":"Elmorsy","year":"2017","journal-title":"Toxicol. Vitro"},{"key":"10.1016\/j.bbrc.2025.152583_bib17","first-page":"278","article-title":"Oxidative stress biomarkers in treatment-responsive and treatment-resistant schizophrenia patients","volume":"43","author":"Buosi","year":"2021","journal-title":"Trends Psychiatry Psychother."},{"key":"10.1016\/j.bbrc.2025.152583_bib18","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.bbrc.2025.152583_bib19","doi-asserted-by":"crossref","DOI":"10.1155\/2016\/9245150","article-title":"Infrared spectroscopy as a tool to study the antioxidant activity of polyphenolic compounds in isolated rat enterocytes","author":"Barraza-Garza","year":"2016","journal-title":"Oxid. Med. Cell. Longev."},{"key":"10.1016\/j.bbrc.2025.152583_bib20","doi-asserted-by":"crossref","first-page":"11","DOI":"10.3390\/antiox11112134","article-title":"Identification of phytogenic compounds with antioxidant action that protect porcine intestinal epithelial cells from Hydrogen Peroxide induced oxidative damage","volume":"11","author":"Wang","year":"2022","journal-title":"Antioxidants"},{"key":"10.1016\/j.bbrc.2025.152583_bib21","doi-asserted-by":"crossref","first-page":"437","DOI":"10.3390\/oxygen2040030","article-title":"RONS and oxidative stress: an overview of basic concepts","volume":"2","author":"Aranda-Rivera","year":"2022","journal-title":"Oxygen"},{"key":"10.1016\/j.bbrc.2025.152583_bib22","first-page":"43","article-title":"Oxidative stress and antioxidant mechanisms in human body","volume":"6","author":"Adwas","year":"2019","journal-title":"J. Appl. Biotech. Bioeng."},{"key":"10.1016\/j.bbrc.2025.152583_bib23","first-page":"11","article-title":"Assessment of antipsychotic-induced cytotoxic effects on isolated CD1 mouse pancreatic beta cells","volume":"17","author":"Doghaither","year":"2023","journal-title":"Int. J. Health Sci."},{"key":"10.1016\/j.bbrc.2025.152583_bib24","first-page":"11","article-title":"Pharmacogenetics of chlorpromazine and its role in the development of antipsychotic-induced parkinsonism","volume":"1","author":"Vaiman","year":"2021","journal-title":"Per. Psychiatry Neurol."},{"key":"10.1016\/j.bbrc.2025.152583_bib25","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1124\/jpet.103.057554","article-title":"Effect of a beta 2-adrenoceptor stimulation on hyperglycemia-induced endothelial dysfunction","volume":"308","author":"Kabat","year":"2004","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.bbrc.2025.152583_bib26","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.toxlet.2005.04.012","article-title":"Inhibition of alpha7-nicotinic acetylcholine receptor expression by arsenite in the vascular endothelial cells","volume":"159","author":"Hsu","year":"2005","journal-title":"Toxicol. Lett."},{"key":"10.1016\/j.bbrc.2025.152583_bib27","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/1750-2187-3-14","article-title":"The neurotransmitter dopamine modulates vascular permeability in the endothelium","volume":"3","author":"Bhattacharya","year":"2008","journal-title":"J. Mol. Signal."},{"key":"10.1016\/j.bbrc.2025.152583_bib28","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1248\/bpb.23.957","article-title":"Local Thrombus Formation in the site of intravenous injection of chlorpromazine: possible colloid-osmotic lysis of the local endothelial cells","volume":"23","author":"Masashi","year":"2000","journal-title":"Biol. Pharm. Bull."},{"key":"10.1016\/j.bbrc.2025.152583_bib29","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1016\/j.biopha.2019.01.020","article-title":"Chlorpromazine toxicity is associated with disruption of cell membrane integrity and initiation of a pro-inflammatory response in the HepaRG hepatic cell line","volume":"111","author":"Morgan","year":"2019","journal-title":"Biomed. Pharmacother."},{"key":"10.1016\/j.bbrc.2025.152583_bib30","doi-asserted-by":"crossref","first-page":"4184","DOI":"10.1021\/ja209917q","article-title":"Kinetics and thermodynamics of chlorpromazine interaction with lipid bilayers: effect of charge and cholesterol","volume":"134","author":"Martins","year":"2012","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.bbrc.2025.152583_bib31","doi-asserted-by":"crossref","first-page":"1406","DOI":"10.18632\/oncotarget.28010","article-title":"Multifaceted effect of chlorpromazine in cancer: implications for cancer treatment","volume":"12","author":"Kamgar-Dayhoff","year":"2021","journal-title":"Oncotarget"},{"key":"10.1016\/j.bbrc.2025.152583_bib32","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.bpc.2008.02.014","article-title":"Human cells and cell membrane molecular models are affected in vitro by chlorpromazine","volume":"135","author":"Suwalsky","year":"2008","journal-title":"Biophys. Chem."},{"key":"10.1016\/j.bbrc.2025.152583_bib33","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1016\/j.toxrep.2024.04.004","article-title":"Adverse hematological profiles associated with chlorpromazine antipsychotic treatment in male rats: preventive and reversal mechanisms of taurine and coenzyme-Q10","volume":"12","author":"Obukohwo","year":"2024","journal-title":"Toxicol. Rep."},{"key":"10.1016\/j.bbrc.2025.152583_bib34","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1080\/13880209.2022.2110128","article-title":"Kuhuang injection exerts a protective effect by activating PPAR-\u03b3 in an in vitro model of chlorpromazine-induced cholestatic liver injury constructed by tissue engineering","volume":"60","author":"Wu","year":"2022","journal-title":"Pharm. Biol."},{"key":"10.1016\/j.bbrc.2025.152583_bib35","first-page":"125","article-title":"La microespectroscop\u00eda de infrarrojo con transformada de Fourier (FTIRM) en el estudio de sistemas biol\u00f3gicos","volume":"52","author":"Barraza-Garza","year":"2013","journal-title":"Rev. Latinoam. Quim."},{"key":"10.1016\/j.bbrc.2025.152583_bib36","doi-asserted-by":"crossref","first-page":"12047","DOI":"10.1021\/ac502667b","article-title":"Microspectroscopy (\u03bcFTIR) reveals co-localization of lipid oxidation and amyloid plaques in human Alzheimer disease brains","volume":"86","author":"Benseny-Cases","year":"2014","journal-title":"Anal. Chem."},{"key":"10.1016\/j.bbrc.2025.152583_bib37","doi-asserted-by":"crossref","first-page":"8140","DOI":"10.1007\/s12035-016-0280-x","article-title":"Neuroprotection by chlorpromazine and promethazine in severe transient and permanent ischemic stroke","volume":"54","author":"Geng","year":"2017","journal-title":"Mol. Neurobiol."},{"key":"10.1016\/j.bbrc.2025.152583_bib38","doi-asserted-by":"crossref","first-page":"265","DOI":"10.23736\/S0390-5616.19.04665-4","article-title":"Low dose concomitant treatment with chlorpromazine and promethazine is safe in acute ischemic stroke","volume":"63","author":"Zhu","year":"2019","journal-title":"J. Neurosurg. Sci."},{"key":"10.1016\/j.bbrc.2025.152583_bib39","doi-asserted-by":"crossref","DOI":"10.3389\/fneur.2021.621476","article-title":"Rapid intervention of chlorpromazine and promethazine for hibernation-like effect in stroke: rationale, design, and protocol for a prospective randomized controlled trial","volume":"12","author":"Lv","year":"2021","journal-title":"Front. Neurol."},{"key":"10.1016\/j.bbrc.2025.152583_bib40","doi-asserted-by":"crossref","first-page":"5944","DOI":"10.1021\/cr200084z","article-title":"Free radical lipid peroxidation: mechanisms and analysis","volume":"111","author":"Yin","year":"2011","journal-title":"Chem. Rev."},{"key":"10.1016\/j.bbrc.2025.152583_bib41","doi-asserted-by":"crossref","DOI":"10.1155\/2014\/360438","article-title":"Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal","volume":"2014","author":"Ayala","year":"2014","journal-title":"Oxid. Med. Cell. Longev."},{"key":"10.1016\/j.bbrc.2025.152583_bib42","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbamem.2021.183726","article-title":"ATR-FTIR spectroscopy probing of structural alterations in the cellular membrane of abscopal liver cells","volume":"1863","author":"Abdelrazzak","year":"2021","journal-title":"Biochim. Biophys. Acta Biomembr."},{"key":"10.1016\/j.bbrc.2025.152583_bib43","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1080\/05704920701829043","article-title":"Fourier transform infrared (FTIR) spectroscopy of biological tissues","volume":"43","author":"Movasaghi","year":"2008","journal-title":"Appl. Spectrosc. Rev."},{"key":"10.1016\/j.bbrc.2025.152583_bib44","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1016\/j.bbabio.2007.06.004","article-title":"Infrared spectroscopy of proteins","volume":"1767","author":"Barth","year":"2007","journal-title":"Biochim. Biophys. Acta"}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X25012987?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X25012987?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T19:48:17Z","timestamp":1772826497000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X25012987"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":44,"alternative-id":["S0006291X25012987"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2025.152583","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The antipsychotic chlorpromazine induces oxidative stress in endothelial cells: an in vitro assessment employing Fourier-transform infrared spectroscopy","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2025.152583","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"152583"}}