{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T16:10:10Z","timestamp":1788883810068,"version":"build-2803163510"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T00:00:00Z","timestamp":1757548800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP19K18361"],"award-info":[{"award-number":["JP19K18361"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP23K08445"],"award-info":[{"award-number":["JP23K08445"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.bbrc.2025.152634","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T02:23:14Z","timestamp":1757643794000},"page":"152634","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Insights into the effects of inhaled drugs on airway epithelium with human airway basal cells"],"prefix":"10.1016","volume":"783","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7069-9334","authenticated-orcid":false,"given":"Fumihiro","family":"Ogawa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kohei","family":"Somekawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aya","family":"Yabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mototsugu","family":"Nishii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7775-4308","authenticated-orcid":false,"given":"Hideshi","family":"Okada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chihiro","family":"Takada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Kaneko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ichiro","family":"Takeuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"7","key":"10.1016\/j.bbrc.2025.152634_bib1","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1513\/pats.200805-041HR","article-title":"Airway epithelial cells: current concepts and challenges","volume":"5","author":"Crystal","year":"2008","journal-title":"Proc. Am. Thorac. Soc."},{"issue":"4","key":"10.1016\/j.bbrc.2025.152634_bib2","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1046\/j.1440-1843.2003.00493.x","article-title":"The airway epithelium: structural and functional properties in health and disease","volume":"8","author":"Knight","year":"2003","journal-title":"Respirology"},{"key":"10.1016\/j.bbrc.2025.152634_bib3","article-title":"Airway epithelium dysfunction in cystic fibrosis and COPD","volume":"2018","author":"De Rose","year":"2018","journal-title":"Mediat. Inflamm."},{"issue":"4","key":"10.1016\/j.bbrc.2025.152634_bib4","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1183\/09031936.00141514","article-title":"The innate immune function of airway epithelial cells in inflammatory lung disease","volume":"45","author":"Hiemstra","year":"2015","journal-title":"Eur. Respir. J."},{"issue":"1","key":"10.1016\/j.bbrc.2025.152634_bib5","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1136\/thorax.56.1.25","article-title":"Ultrastructural examination of bronchial biopsy specimens from children with moderate asthma","volume":"56","author":"Cokugras","year":"2001","journal-title":"Thorax"},{"issue":"2","key":"10.1016\/j.bbrc.2025.152634_bib6","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1164\/ajrccm.163.2.2004039","article-title":"Mild and moderate asthma is associated with airway goblet cell hyperplasia and abnormalities in mucin gene expression","volume":"163","author":"Ordonez","year":"2001","journal-title":"Am. J. Respir. Crit. Care Med."},{"issue":"6","key":"10.1016\/j.bbrc.2025.152634_bib7","doi-asserted-by":"crossref","first-page":"2367","DOI":"10.1172\/JCI84910","article-title":"Epithelial tethering of MUC5AC-rich mucus impairs mucociliary transport in asthma","volume":"126","author":"Bonser","year":"2016","journal-title":"J. Clin. Investig."},{"issue":"6","key":"10.1016\/j.bbrc.2025.152634_bib8","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1165\/ajrcmb.13.6.7576713","article-title":"Analysis of respiratory mucus glycoproteins in asthma: a detailed study from a patient who died in status asthmaticus","volume":"13","author":"Sheehan","year":"1995","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"issue":"7","key":"10.1016\/j.bbrc.2025.152634_bib9","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1183\/13993003\/erj.9.7.1395","article-title":"Continuity of airway goblet cells and intraluminal mucus in the airways of patients with bronchial asthma","volume":"9","author":"Shimura","year":"1996","journal-title":"Eur. Respir. J."},{"issue":"4","key":"10.1016\/j.bbrc.2025.152634_bib10","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1378\/chest.118.4.1142","article-title":"The effect of inflammation on mucociliary clearance in asthma: an overview","volume":"118","author":"Del Donno","year":"2000","journal-title":"Chest"},{"issue":"3","key":"10.1016\/j.bbrc.2025.152634_bib11","doi-asserted-by":"crossref","first-page":"329","DOI":"10.2332\/allergolint.55.329","article-title":"Elimination of IL-13 reverses established goblet cell metaplasia into ciliated epithelia in airway epithelial cell culture","volume":"55","author":"Kondo","year":"2006","journal-title":"Allergol. Int."},{"issue":"12","key":"10.1016\/j.bbrc.2025.152634_bib12","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1172\/JCI200113557","article-title":"IL-13 alters mucociliary differentiation and ciliary beating of human respiratory epithelial cells","volume":"108","author":"Laoukili","year":"2001","journal-title":"J. Clin. Investig."},{"issue":"10","key":"10.1016\/j.bbrc.2025.152634_bib13","first-page":"2914","article-title":"SPDEF is required for mouse pulmonary goblet cell differentiation and regulates a network of genes associated with mucus production","volume":"119","author":"Chen","year":"2009","journal-title":"J. Clin. Investig."},{"issue":"4\u20136","key":"10.1016\/j.bbrc.2025.152634_bib14","doi-asserted-by":"crossref","first-page":"83","DOI":"10.3109\/15419061.2010.551682","article-title":"Interleukin-13 induces mucin 5AC production involving STAT6\/SPDEF in human airway epithelial cells","volume":"17","author":"Yu","year":"2010","journal-title":"Cell Commun. Adhes."},{"issue":"5","key":"10.1016\/j.bbrc.2025.152634_bib15","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1164\/rccm.200903-0392OC","article-title":"T-helper type 2-driven inflammation defines major subphenotypes of asthma","volume":"180","author":"Woodruff","year":"2009","journal-title":"Am. J. Respir. Crit. Care Med."},{"issue":"2","key":"10.1016\/j.bbrc.2025.152634_bib16","doi-asserted-by":"crossref","first-page":"127","DOI":"10.2500\/aap.2011.32.3426","article-title":"Long-term treatment with fluticasone propionate\/salmeterol via diskus improves asthma control versus fluticasone propionate alone","volume":"32","author":"Katial","year":"2011","journal-title":"Allergy Asthma Proc."},{"issue":"5","key":"10.1016\/j.bbrc.2025.152634_bib17","doi-asserted-by":"crossref","first-page":"415","DOI":"10.2500\/aap.2010.31.3362","article-title":"Effect of addition of salmeterol versus doubling the dose of fluticasone propionate on specific airway resistance in children with asthma","volume":"31","author":"Murray","year":"2010","journal-title":"Allergy Asthma Proc."},{"issue":"5","key":"10.1016\/j.bbrc.2025.152634_bib19","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1165\/rcmb.2013-0363OC","article-title":"FOXJ1 prevents cilia growth inhibition by cigarette smoke in human airway epithelium in vitro","volume":"51","author":"Brekman","year":"2014","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"issue":"1","key":"10.1016\/j.bbrc.2025.152634_bib20","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1080\/01926230601060025","article-title":"Cystic fibrosis and other respiratory diseases of impaired mucus clearance","volume":"35","author":"Livraghi","year":"2007","journal-title":"Toxicol. Pathol."},{"issue":"6 Pt 1","key":"10.1016\/j.bbrc.2025.152634_bib21","doi-asserted-by":"crossref","first-page":"1868","DOI":"10.1164\/ajrccm.154.6.8970383","article-title":"Mucociliary clearance in the airways","volume":"154","author":"Wanner","year":"1996","journal-title":"Am. J. Respir. Crit. Care Med."},{"issue":"23","key":"10.1016\/j.bbrc.2025.152634_bib22","doi-asserted-by":"crossref","first-page":"2233","DOI":"10.1056\/NEJMra0910061","article-title":"Airway mucus function and dysfunction","volume":"363","author":"Fahy","year":"2010","journal-title":"N. Engl. J. Med."},{"issue":"4","key":"10.1016\/j.bbrc.2025.152634_bib23","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1002\/path.1652","article-title":"Cilia-related diseases","volume":"204","author":"Afzelius","year":"2004","journal-title":"J. Pathol."},{"issue":"12","key":"10.1016\/j.bbrc.2025.152634_bib24","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0008157","article-title":"Smoking is associated with shortened airway cilia","volume":"4","author":"Leopold","year":"2009","journal-title":"PLoS One"},{"issue":"6","key":"10.1016\/j.bbrc.2025.152634_bib25","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1513\/pats.200603-089MS","article-title":"State of the art. Chronic obstructive pulmonary disease, inflammation, and lung cancer","volume":"3","author":"Brody","year":"2006","journal-title":"Proc. Am. Thorac. Soc."},{"issue":"3","key":"10.1016\/j.bbrc.2025.152634_bib26","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1183\/09031936.00124506","article-title":"Tobacco use in relation to COPD and asthma","volume":"29","author":"Hylkema","year":"2007","journal-title":"Eur. Respir. J."},{"issue":"1","key":"10.1016\/j.bbrc.2025.152634_bib27","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1080\/00039896.1972.10666136","article-title":"Tracheobronchial clearance in smoking-discordant twins","volume":"25","author":"Camner","year":"1972","journal-title":"Arch. Environ. Health"},{"issue":"2","key":"10.1016\/j.bbrc.2025.152634_bib28","first-page":"205","article-title":"Relationship of smoking history and pulmonary function tests to tracheal mucous velocity in nonsmokers, young smokers, ex-smokers, and patients with chronic bronchitis","volume":"117","author":"Goodman","year":"1978","journal-title":"Am. Rev. Respir. Dis."},{"issue":"7","key":"10.1016\/j.bbrc.2025.152634_bib29","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1172\/JCI106624","article-title":"Deposition and clearance of 2 micron particles in the tracheobronchial tree of normal subjects--smokers and nonsmokers","volume":"50","author":"Lourenco","year":"1971","journal-title":"J. Clin. Investig."},{"issue":"8","key":"10.1016\/j.bbrc.2025.152634_bib30","doi-asserted-by":"crossref","first-page":"2243","DOI":"10.1111\/j.1476-5381.2012.01929.x","article-title":"Roflumilast N-oxide, a PDE4 inhibitor, improves cilia motility and ciliated human bronchial epithelial cells compromised by cigarette smoke in vitro","volume":"166","author":"Milara","year":"2012","journal-title":"Br. J. Pharmacol."},{"issue":"6","key":"10.1016\/j.bbrc.2025.152634_bib31","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1165\/rcmb.2009-0297OC","article-title":"Long-term cigarette smoke exposure in a mouse model of ciliated epithelial cell function","volume":"43","author":"Simet","year":"2010","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"10.1016\/j.bbrc.2025.152634_bib32","first-page":"93","article-title":"Mucociliary clearance and airways obstruction in smokers, ex-smokers and normal subjects who never smoked","volume":"139","author":"Vastag","year":"1985","journal-title":"Eur. J. Respir. Dis. Suppl."},{"issue":"8","key":"10.1016\/j.bbrc.2025.152634_bib33","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1016\/j.rmed.2012.04.001","article-title":"Ciliary beating is depressed in nasal cilia from chronic obstructive pulmonary disease subjects","volume":"106","author":"Yaghi","year":"2012","journal-title":"Respir. Med."},{"issue":"4","key":"10.1016\/j.bbrc.2025.152634_bib34","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1177\/1753465810396539","article-title":"The airway epithelium: more than just a structural barrier","volume":"5","author":"Tam","year":"2011","journal-title":"Ther. Adv. Respir. Dis."},{"issue":"2","key":"10.1016\/j.bbrc.2025.152634_bib35","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0116507","article-title":"Activation of NOTCH1 or NOTCH3 signaling skews human airway basal cell differentiation toward a secretory pathway","volume":"10","author":"Gomi","year":"2015","journal-title":"PLoS One"},{"issue":"2","key":"10.1016\/j.bbrc.2025.152634_bib36","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1007\/s12015-016-9707-z","article-title":"Endothelial cell mediated promotion of ciliated cell differentiation of human airway basal cells via insulin and insulin-like growth factor 1 receptor mediated signaling","volume":"13","author":"Gomi","year":"2017","journal-title":"Stem Cell Rev. Rep."},{"issue":"1","key":"10.1016\/j.bbrc.2025.152634_bib37","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1186\/1465-9921-14-135","article-title":"Generation of a human airway epithelium derived basal cell line with multipotent differentiation capacity","volume":"14","author":"Walters","year":"2013","journal-title":"Respir. Res."},{"issue":"299","key":"10.1016\/j.bbrc.2025.152634_bib38","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.aaa1233","article-title":"Automated identification of abnormal respiratory ciliary motion in nasal biopsies","volume":"7","author":"Quinn","year":"2015","journal-title":"Sci. Transl. Med."},{"issue":"6","key":"10.1016\/j.bbrc.2025.152634_bib39","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1165\/rcmb.2009-0244OC","article-title":"Interleukin-13-induced mucous metaplasia increases susceptibility of human airway epithelium to rhinovirus infection","volume":"43","author":"Lachowicz-Scroggins","year":"2010","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"issue":"2","key":"10.1016\/j.bbrc.2025.152634_bib40","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1080\/17425255.2020.1716730","article-title":"A review of the pharmacokinetics of M(3) muscarinic receptor antagonists used for the treatment of asthma","volume":"16","author":"Matera","year":"2020","journal-title":"Expert Opin. Drug Metab. Toxicol."},{"issue":"1","key":"10.1016\/j.bbrc.2025.152634_bib41","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1186\/s12931-021-01862-1","article-title":"Potentiation of long-acting beta(2)-agonist and glucocorticoid responses in human airway epithelial cells by modulation of intracellular cAMP","volume":"22","author":"Kim","year":"2021","journal-title":"Respir. Res."},{"issue":"163","key":"10.1016\/j.bbrc.2025.152634_bib42","doi-asserted-by":"crossref","DOI":"10.1183\/16000617.0112-2021","article-title":"Role of air pollutants in airway epithelial barrier dysfunction in asthma and COPD","volume":"31","author":"Aghapour","year":"2022","journal-title":"Eur. Respir. Rev."},{"issue":"4","key":"10.1016\/j.bbrc.2025.152634_bib43","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1164\/ajrccm.150.4.7921429","article-title":"Effect of eight weeks of treatment with salmeterol on bronchoalveolar lavage inflammatory indices in asthmatics","volume":"150","author":"Gardiner","year":"1994","journal-title":"Am. J. Respir. Crit. Care Med."},{"issue":"1","key":"10.1016\/j.bbrc.2025.152634_bib44","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.alit.2017.08.011","article-title":"Role of airway epithelial barrier dysfunction in pathogenesis of asthma","volume":"67","author":"Gon","year":"2018","journal-title":"Allergol. Int."},{"issue":"12","key":"10.1016\/j.bbrc.2025.152634_bib45","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0260706","article-title":"Budesonide promotes airway epithelial barrier integrity following double-stranded RNA challenge","volume":"16","author":"Rimmer","year":"2021","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.bbrc.2025.152634_bib46","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1186\/s12890-022-01983-3","article-title":"Effects of long-acting muscarinic antagonists on promoting ciliary function in airway epithelium","volume":"22","author":"Katsumata","year":"2022","journal-title":"BMC Pulm. Med."},{"key":"10.1016\/j.bbrc.2025.152634_bib47","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/s13223-020-00472-8","article-title":"Treatment strategies for asthma: reshaping the concept of asthma management","volume":"16","author":"Papi","year":"2020","journal-title":"Allergy Asthma Clin. Immunol."},{"issue":"1","key":"10.1016\/j.bbrc.2025.152634_bib48","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1164\/rccm.202109-2205PP","article-title":"Global initiative for asthma strategy 2021: executive summary and rationale for key changes","volume":"205","author":"Reddel","year":"2022","journal-title":"Am. J. Respir. Crit. Care Med."},{"issue":"4","key":"10.1016\/j.bbrc.2025.152634_bib49","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/j.anai.2021.12.020","article-title":"Understanding the role of long-acting muscarinic antagonists in asthma treatment","volume":"128","author":"Muiser","year":"2022","journal-title":"Ann. Allergy Asthma Immunol."},{"issue":"8","key":"10.1016\/j.bbrc.2025.152634_bib50","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1080\/14656566.2024.2366991","article-title":"Asthma management with triple ICS\/LABA\/LAMA combination to reduce the risk of exacerbation: an umbrella review compliant with the PRIOR statement","volume":"25","author":"Laitano","year":"2024","journal-title":"Expet Opin. Pharmacother."}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X25013506?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X25013506?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T23:00:14Z","timestamp":1773097214000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X25013506"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":49,"alternative-id":["S0006291X25013506"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2025.152634","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Insights into the effects of inhaled drugs on airway epithelium with human airway basal cells","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2025.152634","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"152634"}}