{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T11:00:40Z","timestamp":1793530840374,"version":"4.5.0"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"am","delay-in-days":350,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100020963","name":"Moonshot Research and Development Program","doi-asserted-by":"publisher","award":["JPNP18016"],"award-info":[{"award-number":["JPNP18016"]}],"id":[{"id":"10.13039\/501100020963","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001863","name":"New Energy and Industrial Technology Development Organization","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001863","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24KJ0722"],"award-info":[{"award-number":["24KJ0722"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22H03966"],"award-info":[{"award-number":["22H03966"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25K23574"],"award-info":[{"award-number":["25K23574"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003051","name":"New Energy and Industrial Technology Development Organization","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003051","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJSP2108"],"award-info":[{"award-number":["JPMJSP2108"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.bbrc.2025.152635","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T02:23:46Z","timestamp":1757643826000},"page":"152635","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Promoting plant protoplast fusion via membrane decoration with cell-penetrating peptide-conjugated lipids"],"prefix":"10.1016","volume":"784","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6994-7689","authenticated-orcid":false,"given":"Yuya","family":"Sato","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumiko","family":"Hosaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoko","family":"Niki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Osawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0925-1063","authenticated-orcid":false,"given":"Erika","family":"Yoshihara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shingo","family":"Sakamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobutaka","family":"Mitsuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0709-1000","authenticated-orcid":false,"given":"Yuji","family":"Teramura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2025.152635_bib1","first-page":"115","article-title":"Isolation of tobacco mesophyll cell protoplasts using enzymatic digestion","volume":"9","author":"Takebe","year":"1968","journal-title":"Plant Cell Physiol."},{"issue":"5237","key":"10.1016\/j.bbrc.2025.152635_bib2","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1038\/2251016a0","article-title":"Fusion of isolated plant protoplasts","volume":"225","author":"Power","year":"1970","journal-title":"Nature"},{"issue":"4","key":"10.1016\/j.bbrc.2025.152635_bib3","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1007\/BF00388618","article-title":"A method for high-frequency intergeneric fusion of plant protoplasts","volume":"115","author":"Kao","year":"1974","journal-title":"Planta"},{"issue":"1","key":"10.1016\/j.bbrc.2025.152635_bib4","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1007\/BF00384233","article-title":"High-frequency fusion of plant protoplasts by electric fields","volume":"151","author":"Zimmermann","year":"1981","journal-title":"Planta"},{"key":"10.1016\/j.bbrc.2025.152635_bib5","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/BF02906548","article-title":"Somatic hybrid plants of potato and tomato regenerated from fused protoplasts","volume":"43","author":"Melchers","year":"1978","journal-title":"Carlsberg Res. Commun."},{"key":"10.1016\/j.bbrc.2025.152635_bib6","series-title":"Protoplast Fusion: Genetic Engineering in Higher Plants","author":"Gleba","year":"1984"},{"issue":"5","key":"10.1016\/j.bbrc.2025.152635_bib7","first-page":"1903","article-title":"PEG-mediated symmetric and asymmetric protoplast fusion in sunflower (Helianthus annuus L.)","volume":"49","author":"Kativat","year":"2017","journal-title":"Pakistan J. Bot."},{"issue":"2","key":"10.1016\/j.bbrc.2025.152635_bib8","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1007\/s00299-005-0916-1","article-title":"Electrochemical protoplast fusion in citrus","volume":"24","author":"Olivares-Fuster","year":"2005","journal-title":"Plant Cell Rep."},{"key":"10.1016\/j.bbrc.2025.152635_bib9","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-75983-w","article-title":"Real-time detection of somatic hybrid cells during electrofusion of carrot protoplasts with stably labelled mitochondria","volume":"10","author":"Gieniec","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bbrc.2025.152635_bib10","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1186\/s13007-023-01080-4","article-title":"Early selection of carrot somatic hybrids: a promising tool for species with high regenerative ability","volume":"19","author":"Ma\u0107kowska","year":"2023","journal-title":"Plant Methods"},{"key":"10.1016\/j.bbrc.2025.152635_bib11","doi-asserted-by":"crossref","first-page":"4553","DOI":"10.1038\/s41598-021-83970-y","article-title":"An asymmetric protoplast fusion and screening method for generating celeriac cybrids","volume":"11","author":"Bruznican","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bbrc.2025.152635_bib12","first-page":"79","article-title":"Somatic cell hybridization on rice (Oryza sativa L.) and birdsfoot trefoil (Lotus corniculatus L.)","volume":"44","author":"Nakajo","year":"1994","journal-title":"Breed. Sci."},{"key":"10.1016\/j.bbrc.2025.152635_bib13","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1266\/jjg.60.81","article-title":"Callus formation of somatic hybrid of rice and soybean and characteristics of the hybrid callus","volume":"60","author":"Niizaki","year":"1985","journal-title":"Jpn. J. Genet."},{"issue":"3","key":"10.1016\/j.bbrc.2025.152635_bib14","first-page":"181","article-title":"Production of somatic hybrid plants through protoplast fusion in citrus","volume":"22","author":"Kobayashi","year":"1988","journal-title":"Jpn. Agric. Res. Q."},{"key":"10.1016\/j.bbrc.2025.152635_bib15","doi-asserted-by":"crossref","first-page":"2539","DOI":"10.3390\/plants13182539","article-title":"Modern technologies provide new opportunities for somatic hybridization in the breeding of woody plants","volume":"13","author":"Liu","year":"2024","journal-title":"Plants"},{"key":"10.1016\/j.bbrc.2025.152635_bib16","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1007\/978-1-0716-4204-7_18","article-title":"High-efficient transient gene transfection for transcriptional activity assay using rice protoplasts on large-scale platform","volume":"2869","author":"Sakamoto","year":"2025","journal-title":"Methods Mol. Biol."},{"issue":"2","key":"10.1016\/j.bbrc.2025.152635_bib17","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.biotechadv.2004.09.008","article-title":"Plant protoplasts: status and biotechnological perspectives","volume":"23","author":"Davey","year":"2005","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.bbrc.2025.152635_bib18","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1039\/b913621e","article-title":"Cell surface modification with polymers for biomedical studies","volume":"6","author":"Teramura","year":"2010","journal-title":"Soft Matter"},{"key":"10.1016\/j.bbrc.2025.152635_bib19","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1021\/acsami.6b14584","article-title":"Cell adhesion induced using surface modification with cell-penetrating peptide-conjugated poly(ethylene glycol)-lipid: A new cell glue for 3D cell-based structures","volume":"9","author":"Teramura","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.bbrc.2025.152635_bib20","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.actbio.2016.02.018","article-title":"Heparinization of cell surfaces with short peptide-conjugated PEG\u2013lipid regulates thromboinflammation in transplantation of human MSCs and hepatocytes","volume":"35","author":"Asif","year":"2016","journal-title":"Acta Biomater."},{"key":"10.1016\/j.bbrc.2025.152635_bib21","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1002\/jbm.a.36697","article-title":"Modification of human MSC surface with oligopeptide\u2013PEG\u2013lipids for selective binding to activated endothelium","volume":"107","author":"Noiri","year":"2019","journal-title":"J. Biomed. Mater. Res."},{"key":"10.1016\/j.bbrc.2025.152635_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2020.120113","article-title":"Promotion of cell membrane fusion by cell\u2013cell attachment through cell surface modification with functional peptide\u2013PEG\u2013lipids","volume":"253","author":"Yoshihara","year":"2020","journal-title":"Biomaterials"},{"issue":"5","key":"10.1016\/j.bbrc.2025.152635_bib23","doi-asserted-by":"crossref","first-page":"4598","DOI":"10.1021\/acsabm.1c00335","article-title":"Exogenous cell surface modification with cell-penetrating peptide-conjugated lipids causes spontaneous cell adhesion","volume":"4","author":"Noiri","year":"2021","journal-title":"ACS Appl. Bio Mater."},{"key":"10.1016\/j.bbrc.2025.152635_bib24","doi-asserted-by":"crossref","first-page":"9711","DOI":"10.1021\/acs.langmuir.1c01072","article-title":"Induction of spontaneous liposome adsorption by exogenous surface modification with cell-penetrating peptide-conjugated lipids","volume":"37","author":"Sato","year":"2021","journal-title":"Langmuir"},{"key":"10.1016\/j.bbrc.2025.152635_bib25","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.reth.2022.12.007","article-title":"Surface modulation of extracellular vesicles with cell-penetrating peptide-conjugated lipids for improvement of intracellular delivery to endothelial cells","volume":"22","author":"Huang","year":"2023","journal-title":"Regen Ther"},{"key":"10.1016\/j.bbrc.2025.152635_bib26","doi-asserted-by":"crossref","first-page":"5275","DOI":"10.1039\/D4MA00193A","article-title":"Cell membrane fusion induced by surface modification with cell-penetrating peptide-lipid conjugates that facilitates close contact between distinct membranes","volume":"5","author":"Sato","year":"2024","journal-title":"Mater. Adv."},{"key":"10.1016\/j.bbrc.2025.152635_bib27","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/j.reth.2024.07.006","article-title":"Extracellular vesicle-liposome hybrids via membrane fusion using cell-penetrating peptide-conjugated lipids","volume":"26","author":"Sato","year":"2024","journal-title":"Regen Ther"},{"key":"10.1016\/j.bbrc.2025.152635_bib28","series-title":"Plant Tissue Culture Manual","article-title":"Chemical fusion of protoplasts","author":"Anthony","year":"1995"},{"key":"10.1016\/j.bbrc.2025.152635_bib29","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/0168-9452(93)90069-C","article-title":"Regeneration of somatic hybrid plants between Atropa belladonna L. and Hyoscyamus muticus L","volume":"92","author":"Ahuja","year":"1993","journal-title":"Plant Sci."},{"key":"10.1016\/j.bbrc.2025.152635_bib30","doi-asserted-by":"crossref","first-page":"3018","DOI":"10.1021\/acs.nanolett.2c00216","article-title":"Promoting cell fusion by polyvalent DNA ligands","volume":"22","author":"Gao","year":"2022","journal-title":"Nano Lett."},{"issue":"2","key":"10.1016\/j.bbrc.2025.152635_bib31","doi-asserted-by":"crossref","first-page":"295","DOI":"10.3390\/plants13020295","article-title":"Microfluidic single-cell study on Arabidopsis thaliana protoplast fusion\u2014new insights on timescales and reversibilities","volume":"13","author":"Seidel","year":"2024","journal-title":"Plants"},{"key":"10.1016\/j.bbrc.2025.152635_bib32","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1007\/BF00269554","article-title":"Factors affecting protoplast electrofusion efficiency","volume":"6","author":"Nea","year":"1987","journal-title":"Plant Cell Rep."},{"key":"10.1016\/j.bbrc.2025.152635_bib33","doi-asserted-by":"crossref","DOI":"10.1039\/D5TB01318F","article-title":"Induction of intercellular interaction and cell fusion by cell-penetrating peptide-conjugated lipids","author":"Sato","year":"2025","journal-title":"J. Mater. Chem. B"}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X25013518?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X25013518?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T08:32:01Z","timestamp":1780302721000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X25013518"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":33,"alternative-id":["S0006291X25013518"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2025.152635","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Promoting plant protoplast fusion via membrane decoration with cell-penetrating peptide-conjugated lipids","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2025.152635","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"152635"}}