{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T23:42:58Z","timestamp":1773963778987,"version":"3.50.1"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100031256","name":"Symbiosis International University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100031256","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001407","name":"India Ministry of Science & Technology Department of Biotechnology","doi-asserted-by":"publisher","award":["BT\/PR41465\/MED\/31\/435\/2020"],"award-info":[{"award-number":["BT\/PR41465\/MED\/31\/435\/2020"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.bbrc.2026.153260","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T23:58:36Z","timestamp":1767916716000},"page":"153260","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Knockdown of histone H2A deubiquitinase BAP1 in human embryonic stem cells restricts their differentiation repertoire but is dispensable for maintaining their undifferentiated state"],"prefix":"10.1016","volume":"799","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5967-3694","authenticated-orcid":false,"given":"Dharitree","family":"Samal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5858-2232","authenticated-orcid":false,"given":"Prasad","family":"Pethe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.bbrc.2026.153260_bib1","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.cell.2006.07.024","article-title":"Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors","volume":"126","author":"Takahashi","year":"2006","journal-title":"Cell"},{"issue":"6","key":"10.1016\/j.bbrc.2026.153260_bib2","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1016\/j.cell.2005.08.020","article-title":"Core transcriptional regulatory circuitry in human embryonic stem cells","volume":"122","author":"Boyer","year":"2005","journal-title":"Cell"},{"issue":"1","key":"10.1016\/j.bbrc.2026.153260_bib3","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1016\/j.bbrc.2009.11.134","article-title":"Chromatin states of core pluripotency-associated genes in pluripotent, multipotent and differentiated cells","volume":"391","author":"Barrand","year":"2010","journal-title":"Biochem. Biophys. Res. Commun."},{"issue":"7","key":"10.1016\/j.bbrc.2026.153260_bib4","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1242\/dev.128314","article-title":"Lineage specification in the mouse preimplantation embryo","volume":"143","author":"Chazaud","year":"2016","journal-title":"Development (Cambridge, England)"},{"issue":"4","key":"10.1016\/j.bbrc.2026.153260_bib5","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a035634","article-title":"Specification of the first mammalian cell lineages in vivo and in vitro","volume":"12","author":"White","year":"2020","journal-title":"Cold Spring Harbor Perspect. Biol."},{"issue":"11","key":"10.1016\/j.bbrc.2026.153260_bib6","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.1038\/sj.emboj.7601144","article-title":"Structure and E3-ligase activity of the ring-ring complex of polycomb proteins Bmi1 and Ring1b","volume":"25","author":"Buchwald","year":"2006","journal-title":"EMBO J."},{"issue":"1","key":"10.1016\/j.bbrc.2026.153260_bib7","doi-asserted-by":"crossref","first-page":"5947","DOI":"10.1038\/s41467-020-19722-9","article-title":"Polycomb group-mediated histone H2A monoubiquitination in epigenome regulation and nuclear processes","volume":"11","author":"Barbour","year":"2020","journal-title":"Nat. Commun."},{"issue":"9","key":"10.1016\/j.bbrc.2026.153260_bib8","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.aax1738","article-title":"BAP1 regulates epigenetic switch from pluripotency to differentiation in developmental lineages giving rise to BAP1-mutant cancers","volume":"5","author":"Kuznetsov","year":"2019","journal-title":"Sci. Adv."},{"key":"10.1016\/j.bbrc.2026.153260_bib9","article-title":"Regulation of B lymphocyte development by histone H2A deubiquitinase BAP1","volume":"12","author":"Lin","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.bbrc.2026.153260_bib10","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.63254","article-title":"BAP1\/ASXL complex modulation regulates epithelial-mesenchymal transition during trophoblast differentiation and invasion","volume":"10","author":"Perez-Garcia","year":"2021","journal-title":"eLife"},{"issue":"4","key":"10.1016\/j.bbrc.2026.153260_bib11","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0035295","article-title":"Germline BAP1 inactivation is preferentially associated with metastatic ocular melanoma and cutaneous-ocular melanoma families","volume":"7","author":"Njauw","year":"2012","journal-title":"PLoS One"},{"issue":"8","key":"10.1016\/j.bbrc.2026.153260_bib12","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1158\/2159-8290.CD-19-1220","article-title":"Biological mechanisms and clinical significance of BAP1 mutations in human cancer","volume":"10","author":"Carbone","year":"2020","journal-title":"Cancer Discov."},{"key":"10.1016\/j.bbrc.2026.153260_bib13","series-title":"In Vitro Cellular & Developmental Biology Animal","article-title":"Histone H2A deubiquitinase BAP1 is essential for endothelial cell differentiation from human pluripotent stem cells","author":"Shastry","year":"2024"},{"issue":"3","key":"10.1016\/j.bbrc.2026.153260_bib14","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1089\/scd.2008.0234","article-title":"Derivation and characterization of two genetically unique human embryonic stem cell lines on in-house-derived human feeders","volume":"18","author":"Kumar","year":"2009","journal-title":"Stem Cell. Dev."},{"issue":"6","key":"10.1016\/j.bbrc.2026.153260_bib15","doi-asserted-by":"crossref","first-page":"838","DOI":"10.4103\/ijmr.IJMR_424_15","article-title":"Feeder & basic fibroblast growth factor-free culture of human embryonic stem cells: role of conditioned medium from immortalized human feeders","volume":"144","author":"Teotia","year":"2016","journal-title":"Indian J. Med. Res."},{"issue":"1","key":"10.1016\/j.bbrc.2026.153260_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.yexcr.2020.112294","article-title":"PRC1 catalytic unit RING1B regulates early neural differentiation of human pluripotent stem cells","volume":"396","author":"Desai","year":"2020","journal-title":"Exp. Cell Res."},{"issue":"2\u20133","key":"10.1016\/j.bbrc.2026.153260_bib17","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1387\/ijdb.082690mv","article-title":"Role of polycomb proteins Ring1A and Ring1B in the epigenetic regulation of gene expression","volume":"53","author":"Vidal","year":"2009","journal-title":"Int. J. Dev. Biol."},{"issue":"4","key":"10.1016\/j.bbrc.2026.153260_bib18","first-page":"364","article-title":"EZH2: a pivotal regulator in controlling cell differentiation","volume":"4","author":"Chen","year":"2012","journal-title":"Am. J. Tourism Res."},{"issue":"1","key":"10.1016\/j.bbrc.2026.153260_bib19","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1038\/cr.2011.179","article-title":"Oct4 links multiple epigenetic pathways to the pluripotency network","volume":"22","author":"Ding","year":"2012","journal-title":"Cell Res."},{"issue":"6","key":"10.1016\/j.bbrc.2026.153260_bib20","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1128\/MCB.01105-14","article-title":"Pluripotency transcription factor Oct4 mediates stepwise nucleosome demethylation and depletion","volume":"35","author":"Shakya","year":"2015","journal-title":"Mol. Cell Biol."},{"issue":"18","key":"10.1016\/j.bbrc.2026.153260_bib21","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.1101\/gad.268151.115","article-title":"The E3 ubiquitin ligase activity of RING1B is not essential for early mouse development","volume":"29","author":"llingworth","year":"2015","journal-title":"Genes Dev."},{"issue":"20","key":"10.1016\/j.bbrc.2026.153260_bib22","doi-asserted-by":"crossref","first-page":"4061","DOI":"10.1038\/sj.emboj.7600402","article-title":"Suz12 is essential for mouse development and for EZH2 histone methyltransferase activity","volume":"23","author":"Pasini","year":"2004","journal-title":"EMBO J."},{"issue":"10","key":"10.1016\/j.bbrc.2026.153260_bib23","doi-asserted-by":"crossref","first-page":"3769","DOI":"10.1128\/MCB.01432-06","article-title":"The polycomb group protein Suz12 is required for embryonic stem cell differentiation","volume":"27","author":"Pasini","year":"2007","journal-title":"Mol. Cell Biol."},{"issue":"13","key":"10.1016\/j.bbrc.2026.153260_bib24","doi-asserted-by":"crossref","first-page":"4330","DOI":"10.1128\/MCB.21.13.4330-4336.2001","article-title":"The polycomb-group gene Ezh2 is required for early mouse development","volume":"21","author":"O'Carroll","year":"2001","journal-title":"Mol. Cell Biol."},{"issue":"1","key":"10.1016\/j.bbrc.2026.153260_bib25","doi-asserted-by":"crossref","first-page":"44","DOI":"10.15283\/ijsc22138","article-title":"Embryonic stem cells lacking DNA methyltransferases differentiate into neural stem cells that are defective in self-renewal","volume":"16","author":"Seo","year":"2023","journal-title":"Int. J. Stem Cells"},{"key":"10.1016\/j.bbrc.2026.153260_bib26","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1038\/ng.3258","article-title":"Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells","volume":"47","author":"Liao","year":"2015","journal-title":"Nat. Genet."},{"issue":"9\u201310","key":"10.1016\/j.bbrc.2026.153260_bib27","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1101\/gad.347005.120","article-title":"BAP1 constrains pervasive H2AK119ub1 to control the transcriptional potential of the genome","volume":"35","author":"Fursova","year":"2021","journal-title":"Genes Dev."},{"issue":"2","key":"10.1016\/j.bbrc.2026.153260_bib28","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1083\/jcb.200612127","article-title":"Ring1B is crucial for the regulation of developmental control genes and PRC1 proteins but not X inactivation in embryonic cells","volume":"178","author":"Leeb","year":"2007","journal-title":"J. Cell Biol."},{"issue":"5","key":"10.1016\/j.bbrc.2026.153260_bib29","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0002235","article-title":"Ubiquitin E3 ligase Ring1b\/Rnf2 of polycomb repressive complex 1 contributes to stable maintenance of mouse embryonic stem cells","volume":"3","author":"van der Stoop","year":"2008","journal-title":"PLoS One"}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X26000239?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X26000239?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T21:08:35Z","timestamp":1773954515000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X26000239"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":29,"alternative-id":["S0006291X26000239"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2026.153260","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Knockdown of histone H2A deubiquitinase BAP1 in human embryonic stem cells restricts their differentiation repertoire but is dispensable for maintaining their undifferentiated state","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2026.153260","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"153260"}}