{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T04:47:24Z","timestamp":1782276444469,"version":"3.54.5"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFE0132700"],"award-info":[{"award-number":["2022YFE0132700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["82320108019"],"award-info":[{"award-number":["82320108019"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["82360698"],"award-info":[{"award-number":["82360698"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["42376112"],"award-info":[{"award-number":["42376112"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004607","name":"Natural Science Foundation of Guangxi Province","doi-asserted-by":"publisher","award":["2025GXNSFAA069562"],"award-info":[{"award-number":["2025GXNSFAA069562"]}],"id":[{"id":"10.13039\/501100004607","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004607","name":"Natural Science Foundation of Guangxi Province","doi-asserted-by":"publisher","award":["2024GXNSFDA999003"],"award-info":[{"award-number":["2024GXNSFDA999003"]}],"id":[{"id":"10.13039\/501100004607","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002701","name":"Ministry of Education","doi-asserted-by":"publisher","award":["TMIT 2024Y02"],"award-info":[{"award-number":["TMIT 2024Y02"]}],"id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018593","name":"Bagui Scholars Program of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170534"],"award-info":[{"award-number":["32170534"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2026,4]]},"DOI":"10.1016\/j.bbrc.2026.153416","type":"journal-article","created":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T17:00:05Z","timestamp":1770397205000},"page":"153416","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Efficient hydrazide-mediated total synthesis of M\u00f6bius cyclotides from Viola japonica and elucidation of the antibacterial mechanism of Vija 31"],"prefix":"10.1016","volume":"807","author":[{"given":"Binghui","family":"Ruan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiongwen","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongting","family":"Zhangsun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulan","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6395-4213","authenticated-orcid":false,"given":"Yong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.bbrc.2026.153416_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.phytochem.2021.112749","article-title":"Tropical vibes from Sri Lanka - cyclotides from Viola betonicifolia by transcriptome and mass spectrometry analysis","volume":"187","author":"Rajendran","year":"2021","journal-title":"Phytochemistry"},{"key":"10.1016\/j.bbrc.2026.153416_bib2","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1016\/j.toxicon.2010.02.021","article-title":"Discovery and applications of the plant cyclotides","volume":"56","author":"Craik","year":"2010","journal-title":"Toxicon"},{"key":"10.1016\/j.bbrc.2026.153416_bib3","doi-asserted-by":"crossref","first-page":"5965","DOI":"10.1021\/bi049711q","article-title":"Thermal, chemical, and enzymatic stability of the cyclotide kalata B1: the importance of the cyclic cystine knot","volume":"43","author":"Colgrave","year":"2004","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbrc.2026.153416_bib4","doi-asserted-by":"crossref","first-page":"12375","DOI":"10.1021\/acs.chemrev.9b00402","article-title":"Cyclotides: from structure to function","volume":"119","author":"de Veer","year":"2019","journal-title":"Chem. Rev."},{"key":"10.1016\/j.bbrc.2026.153416_bib5","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S0378-8741(99)00175-0","article-title":"Oldenlandia affinis (R&S) DC. A plant containing uteroactive peptides used in African traditional medicine","volume":"70","author":"Gran","year":"2000","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.bbrc.2026.153416_bib6","article-title":"Enabling efficient folding and high-resolution crystallographic analysis of bracelet cyclotides","volume":"26","author":"Huang","year":"2021","journal-title":"Molecules"},{"key":"10.1016\/j.bbrc.2026.153416_bib7","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.phytochem.2018.04.014","article-title":"PepSAVI-MS reveals anticancer and antifungal cycloviolacins in Viola odorata","volume":"152","author":"Parsley","year":"2018","journal-title":"Phytochemistry"},{"key":"10.1016\/j.bbrc.2026.153416_bib8","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/S0014-5793(01)02456-5","article-title":"Antibiotic activities of peptides, hydrogen peroxide and peroxynitrite in plant defence","volume":"498","author":"Garc\u00eda-Olmedo","year":"2001","journal-title":"FEBS Lett."},{"key":"10.1016\/j.bbrc.2026.153416_bib9","doi-asserted-by":"crossref","first-page":"3290","DOI":"10.1096\/fj.11-184291","article-title":"Plant storage proteins with antimicrobial activity: novel insights into plant defense mechanisms","volume":"25","author":"C\u00e2ndido Ede","year":"2011","journal-title":"FASEB J."},{"key":"10.1016\/j.bbrc.2026.153416_bib10","doi-asserted-by":"crossref","first-page":"1964","DOI":"10.1093\/jac\/dkq220","article-title":"The cyclotide cycloviolacin O2 from Viola odorata has potent bactericidal activity against Gram-negative bacteria","volume":"65","author":"Pr\u00e4nting","year":"2010","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.bbrc.2026.153416_bib11","doi-asserted-by":"crossref","first-page":"2537","DOI":"10.1021\/acs.jnatprod.9b00359","article-title":"Viola \"inconspicua\" no more: an analysis of antibacterial cyclotides","volume":"82","author":"Parsley","year":"2019","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.bbrc.2026.153416_bib12","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1021\/acsinfecdis.4c00957","article-title":"Small and versatile cyclotides as anti-infective agents","volume":"11","author":"C\u00e2ndido","year":"2025","journal-title":"ACS Infect. Dis."},{"key":"10.1016\/j.bbrc.2026.153416_bib13","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1002\/bip.20886","article-title":"Cyclotides as natural anti-HIV agents","volume":"90","author":"Ireland","year":"2008","journal-title":"Biopolymers"},{"key":"10.1016\/j.bbrc.2026.153416_bib14","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/s00018-009-0159-3","article-title":"Cyclotides: macrocyclic peptides with applications in drug design and agriculture","volume":"67","author":"Craik","year":"2010","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.bbrc.2026.153416_bib15","doi-asserted-by":"crossref","first-page":"2152","DOI":"10.1016\/j.drudis.2019.09.010","article-title":"Pharmaceutical applications of cyclotides","volume":"24","author":"Ojeda","year":"2019","journal-title":"Drug Discov. Today"},{"key":"10.1016\/j.bbrc.2026.153416_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.peptides.2020.170430","article-title":"An insight into biological activities of native cyclotides for potential applications in agriculture and pharmaceutics","volume":"135","author":"Grover","year":"2021","journal-title":"Peptides"},{"key":"10.1016\/j.bbrc.2026.153416_bib17","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1016\/j.peptides.2011.06.016","article-title":"Isolation and characterization of cytotoxic cyclotides from Viola philippica","volume":"32","author":"He","year":"2011","journal-title":"Peptides"},{"key":"10.1016\/j.bbrc.2026.153416_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.plaphy.2024.109300","article-title":"Integrative transcriptome and mass spectrometry analysis reveals novel cyclotides with antimicrobial and cytotoxic activities from Viola arcuata","volume":"219","author":"Tang","year":"2025","journal-title":"Plant Physiol. Biochem."},{"key":"10.1016\/j.bbrc.2026.153416_bib19","doi-asserted-by":"crossref","first-page":"9733","DOI":"10.1038\/s41598-024-60246-9","article-title":"Characterization and evaluation of cytotoxic and antimicrobial activities of cyclotides from Viola japonica","volume":"14","author":"Lian","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.bbrc.2026.153416_bib20","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1006\/jmbi.1999.3383","article-title":"Plant cyclotides: a unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif","volume":"294","author":"Craik","year":"1999","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.bbrc.2026.153416_bib21","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1021\/acs.jnatprod.0c01069","article-title":"Cyclotides from Brazilian Palicourea sessilis and their effects on human lymphocytes","volume":"84","author":"Pinto","year":"2021","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.bbrc.2026.153416_bib22","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1002\/psc.2749","article-title":"Intramolecular acyl transfer in peptide and protein ligation and synthesis","volume":"21","author":"Tailhades","year":"2015","journal-title":"J. Pept. Sci."},{"key":"10.1016\/j.bbrc.2026.153416_bib23","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1039\/C9CC07783A","article-title":"On-resin synthesis of cyclic peptides via tandem N-to-S acyl migration and intramolecular thiol additive-free native chemical ligation","volume":"56","author":"Serra","year":"2020","journal-title":"Chem. Commun."},{"key":"10.1016\/j.bbrc.2026.153416_bib24","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1039\/C4CS00246F","article-title":"Peptide stapling techniques based on different macrocyclisation chemistries","volume":"44","author":"Lau","year":"2015","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.bbrc.2026.153416_bib25","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1021\/co300136j","article-title":"Global analysis of peptide cyclization efficiency","volume":"15","author":"Thakkar","year":"2013","journal-title":"ACS Comb. Sci."},{"key":"10.1016\/j.bbrc.2026.153416_bib26","doi-asserted-by":"crossref","first-page":"5538","DOI":"10.1021\/jo500699m","article-title":"Fmoc-based synthesis of disulfide-rich cyclic peptides","volume":"79","author":"Cheneval","year":"2014","journal-title":"J. Org. Chem."},{"key":"10.1016\/j.bbrc.2026.153416_bib27","doi-asserted-by":"crossref","first-page":"3985","DOI":"10.1002\/anie.200600702","article-title":"Kinetically controlled ligation for the convergent chemical synthesis of proteins","volume":"45","author":"Bang","year":"2006","journal-title":"Angew Chem. Int. Ed. Engl."},{"key":"10.1016\/j.bbrc.2026.153416_bib28","doi-asserted-by":"crossref","first-page":"7645","DOI":"10.1002\/anie.201100996","article-title":"Protein chemical synthesis by ligation of peptide hydrazides","volume":"50","author":"Fang","year":"2011","journal-title":"Angew Chem. Int. Ed. Engl."},{"key":"10.1016\/j.bbrc.2026.153416_bib29","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1126\/science.7973629","article-title":"Synthesis of proteins by native chemical ligation","volume":"266","author":"Dawson","year":"1994","journal-title":"Science"},{"key":"10.1016\/j.bbrc.2026.153416_bib30","doi-asserted-by":"crossref","first-page":"10606","DOI":"10.1021\/bi990605b","article-title":"Chemical synthesis and folding pathways of large cyclic polypeptides: studies of the cystine knot polypeptide kalata B1","volume":"38","author":"Daly","year":"1999","journal-title":"Biochemistry"},{"key":"10.1016\/j.bbrc.2026.153416_bib31","doi-asserted-by":"crossref","first-page":"6037","DOI":"10.1002\/anie.201915358","article-title":"Synthesis of disulfide surrogate peptides incorporating large-span surrogate bridges through a native-chemical-ligation-assisted diaminodiacid strategy","volume":"59","author":"Qu","year":"2020","journal-title":"Angew Chem. Int. Ed. Engl."},{"key":"10.1016\/j.bbrc.2026.153416_bib32","doi-asserted-by":"crossref","first-page":"1500","DOI":"10.1039\/C4OB02260B","article-title":"Accelerated Fmoc solid-phase synthesis of peptides with aggregation-disrupting backbones","volume":"13","author":"Huang","year":"2015","journal-title":"Org. Biomol. Chem."},{"key":"10.1016\/j.bbrc.2026.153416_bib33","doi-asserted-by":"crossref","first-page":"16879","DOI":"10.1021\/acs.jmedchem.2c01674","article-title":"Membrane-targeting neolignan-antimicrobial peptide mimic conjugates to combat methicillin-resistant Staphylococcus aureus (MRSA) infections","volume":"65","author":"Yang","year":"2022","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bbrc.2026.153416_bib34","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.actbio.2023.04.030","article-title":"Boosting stability and therapeutic potential of proteolysis-resistant antimicrobial peptides by end-tagging \u03b2-naphthylalanine","volume":"164","author":"He","year":"2023","journal-title":"Acta Biomater."},{"key":"10.1016\/j.bbrc.2026.153416_bib35","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1038\/nature14098","article-title":"A new antibiotic kills pathogens without detectable resistance","volume":"517","author":"Ling","year":"2015","journal-title":"Nature"},{"key":"10.1016\/j.bbrc.2026.153416_bib36","doi-asserted-by":"crossref","first-page":"10523","DOI":"10.1021\/acs.jmedchem.2c00661","article-title":"Lysine-tethered stable bicyclic cationic antimicrobial peptide combats bacterial infection in vivo","volume":"65","author":"He","year":"2022","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bbrc.2026.153416_bib37","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1021\/acsami.9b19927","article-title":"Design of heptad repeat amphiphiles based on database filtering and structure-function relationships to combat drug-resistant fungi and biofilms","volume":"12","author":"Tan","year":"2020","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.bbrc.2026.153416_bib38","article-title":"Alphafold 3.0: the AI protein predictor gets an upgrade","author":"Thompson","year":"2024","journal-title":"Nature"},{"key":"10.1016\/j.bbrc.2026.153416_bib39","doi-asserted-by":"crossref","first-page":"1254","DOI":"10.1021\/acs.jmedchem.2c01396","article-title":"Novel Feleucin-K3-Derived peptides modified with Sulfono-\u03b3-AA building blocks targeting Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus infections","volume":"66","author":"Guo","year":"2023","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.bbrc.2026.153416_bib40","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.bbrc.2026.153416_bib41","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1146\/annurev.bi.39.070170.004205","article-title":"Chemical synthesis of peptides and proteins","volume":"39","author":"Marglin","year":"1970","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.bbrc.2026.153416_bib42","doi-asserted-by":"crossref","first-page":"4801","DOI":"10.1093\/jxb\/erw210","article-title":"Discovery, structure, function, and applications of cyclotides: circular proteins from plants","volume":"67","author":"Weidmann","year":"2016","journal-title":"J. Exp. Bot."},{"key":"10.1016\/j.bbrc.2026.153416_bib43","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1093\/clinids\/10.4.770","article-title":"Roles of porin and beta-lactamase in beta-lactam resistance of Pseudomonas aeruginosa","volume":"10","author":"Hancock","year":"1988","journal-title":"Rev. Infect. Dis."},{"key":"10.1016\/j.bbrc.2026.153416_bib44","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.40171","article-title":"The Acinetobacter baumannii mla system and glycerophospholipid transport to the outer membrane","volume":"8","author":"Kamischke","year":"2019","journal-title":"eLife"},{"key":"10.1016\/j.bbrc.2026.153416_bib45","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1016\/j.phytochem.2007.10.023","article-title":"The alpine violet, Viola biflora, is a rich source of cyclotides with potent cytotoxicity","volume":"69","author":"Herrmann","year":"2008","journal-title":"Phytochemistry"}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X26001804?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X26001804?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T04:08:34Z","timestamp":1782274114000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X26001804"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":45,"alternative-id":["S0006291X26001804"],"URL":"https:\/\/doi.org\/10.1016\/j.bbrc.2026.153416","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Efficient hydrazide-mediated total synthesis of M\u00f6bius cyclotides from Viola japonica and elucidation of the antibacterial mechanism of Vija 31","name":"articletitle","label":"Article Title"},{"value":"Biochemical and Biophysical Research Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.bbrc.2026.153416","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"153416"}}